Restriction map for
pF1KB7244
(Circular) (Six-base) MAPSORT of: pF1KB7244.seq Check: 3213 from: 1 to: 4205
pF1KB7244 4205 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 368 368
Size: 4205
AccI GT'mk_AC
Cuts at: 1243 1243
Size: 4205
Acc65I G'GTAC_C
Cuts at: 658 1221 658
Size: 563 3642
AclI AA'CG_TT
Cuts at: 4045 4045
Size: 4205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2462 3209 3477 2030
Size: 432 747 268 2758
Fragments arranged by size:
2758 747 432 268
AflIII A'CryG_T
Cuts at: 1557 2661 3638 1557
Size: 1104 977 2124
Fragments arranged by size:
2124 1104 977
AgeI A'CCGG_T
Cuts at: 2593 2593
Size: 4205
AleI CACnn'nnGTG
Cuts at: 724 724
Size: 4205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 637 637
Size: 4205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 136 168 397 429 103
Size: 32 1 32 229 32 3879
Fragments arranged by size:
3879 229 32 32 32 1
AlwNI CAG_nnn'CTG
Cuts at: 1510 3077 1510
Size: 1567 2638
ApaI G_GGCC'C
Cuts at: 222 222
Size: 4205
ApaLI G'TGCA_C
Cuts at: 532 2975 532
Size: 2443 1762
ApoI r'AATT_y
Cuts at: 1209 3558 3814 1209
Size: 2349 256 1600
Fragments arranged by size:
2349 1600 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 156 221 1225 156
Size: 65 1004 3136
Fragments arranged by size:
3136 1004 65
BamHI G'GATC_C
Cuts at: 1124 1230 1124
Size: 106 4099
BanI G'GyrC_C
Cuts at: 204 234 557 658 700 1084 1221 1635 1883
Size: 30 323 101 42 384 137 414 248
Cuts at: 1883 1918 204
Size: 35 2491
Fragments arranged by size:
2491 414 384 323 248 137 101 42
35 30
BanII G_rGCy'C
Cuts at: 116 222 547 686 947 1081 1219 2249 116
Size: 106 325 139 261 134 138 1030 2072
Fragments arranged by size:
2072 1030 325 261 139 138 134 106
BbeI G_GCGC'C
Cuts at: 208 561 704 1639 1887 208
Size: 353 143 935 248 2526
Fragments arranged by size:
2526 935 353 248 143
BbsI GAAGACnn'nnnn_
Cuts at: 328 480 699 328
Size: 152 219 3834
Fragments arranged by size:
3834 219 152
BciVI GTATCCnnnnn_n'
Cuts at: 2103 2864 4205 2103
Size: 761 1341 2103
Fragments arranged by size:
2103 1341 761
BclI T'GATC_A
Cuts at: 1725 1725
Size: 4205
BglI GCCn_nnn'nGGC
Cuts at: 580 690 580
Size: 110 4095
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 541 1317 541
Size: 776 3429
Bme1580I G_kGCm'C
Cuts at: 222 239 536 1830 1923 2979 222
Size: 17 297 1294 93 1056 1448
Fragments arranged by size:
1448 1294 1056 297 93 17
BmgBI CAC'GTC
Cuts at: 645 645
Size: 4205
BmrI ACTGGGnnnn_n'
Cuts at: 1417 1498 1833 3977 1417
Size: 81 335 2144 1645
Fragments arranged by size:
2144 1645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 966 2572 966
Size: 1606 2599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 191
Size: 4205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1381 2260 2752 3050 1381
Size: 879 492 298 2536
Fragments arranged by size:
2536 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3909 3909
Size: 4205
BsaAI yAC'GTr
Cuts at: 297 1558 297
Size: 1261 2944
BsaBI GATnn'nnATC
Cuts at: 6 1719 3756 6
Size: 1713 2037 455
Fragments arranged by size:
2037 1713 455
BsaHI Gr'CG_yC
Cuts at: 205 365 467 558 701 1100 1636 1884 2586
Size: 160 102 91 143 399 536 248 702
Cuts at: 2586 4082 205
Size: 1496 328
Fragments arranged by size:
1496 702 536 399 328 248 160 143
102 91
BsaWI w'CCGG_w
Cuts at: 1394 1915 2593 2867 3014 3425 1394
Size: 521 678 274 147 411 2174
Fragments arranged by size:
2174 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 136 166 103
Size: 30 3 30 4142
Fragments arranged by size:
4142 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 141 141
Size: 4205
BseYI C'CCAG_C
Cuts at: 123 136 270 378 729 1630 2388 2965 3461
Size: 13 134 108 351 901 758 577 496
Cuts at: 3461 123
Size: 867
Fragments arranged by size:
901 867 758 577 496 351 134 108
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 667
Size: 4205
BsiEI CG_ry'CG
Cuts at: 104 266 725 1747 1793 3001 104
Size: 162 459 1022 46 1208 1308
Fragments arranged by size:
1308 1208 1022 459 162 46
BsiHKAI G_wGCw'C
Cuts at: 536 547 1219 1997 2187 2979 536
Size: 11 672 778 190 792 1762
Fragments arranged by size:
1762 792 778 672 190 11
Bsp1286I G_dGCh'C
Cuts at: 116 222 239 536 547 686 947 1081 1219
Size: 106 17 297 11 139 261 134 138
Cuts at: 1219 1830 1923 1997 2187 2249 2979 116
Size: 611 93 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 297 261 190 139 138
134 106 93 74 62 17 11
BspEI T'CCGG_A
Cuts at: 3425 3425
Size: 4205
BspHI T'CATG_A
Cuts at: 452 503 3381 452
Size: 51 2878 1276
Fragments arranged by size:
2878 1276 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 257 670 779 1255 1469 1771 2152 257
Size: 413 109 476 214 302 381 2310
Fragments arranged by size:
2310 476 413 381 302 214 109
BsrBI CCG'CTC
Cuts at: 50 627 837 2497 2551 4035 4200 50
Size: 577 210 1660 54 1484 165 55
Fragments arranged by size:
1660 1484 577 210 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2117 2117
Size: 4205
BsrFI r'CCGG_y
Cuts at: 201 955 2203 2384 2593 201
Size: 754 1248 181 209 1813
Fragments arranged by size:
1813 1248 754 209 181
BsrGI T'GTAC_A
Cuts at: 371 371
Size: 4205
BssSI C'ACGA_G
Cuts at: 2476 2834 2476
Size: 358 3847
BstAPI GCAn_nnn'nTGC
Cuts at: 1582 1582
Size: 4205
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstXI CCAn_nnnn'nTGG
Cuts at: 1197 1197
Size: 4205
BstYI r'GATC_y
Cuts at: 1 1124 1230 1697 1720 2055 2301 3313 3399
Size: 1123 106 467 23 335 246 1012 86
Cuts at: 3399 3411 3757 1
Size: 12 346 449
Fragments arranged by size:
1123 1012 467 449 346 335 246 106
86 23 12
BtgI C'CryG_G
Cuts at: 107 590 686 926 1103 107
Size: 483 96 240 177 3209
Fragments arranged by size:
3209 483 240 177 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 362 533 1448 362
Size: 171 915 3119
Fragments arranged by size:
3119 915 171
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DrdI GACnn_nn'nnGTC
Cuts at: 1911 2769 1911
Size: 858 3347
EaeI y'GGCC_r
Cuts at: 263 359 395 572 676 722 923 1790 1964
Size: 96 36 177 104 46 201 867 174
Cuts at: 1964 2355 2382 3727 263
Size: 391 27 1345 741
Fragments arranged by size:
1345 867 741 391 201 177 174 104
96 46 36 27
EagI C'GGCC_G
Cuts at: 263 722 1790 263
Size: 459 1068 2678
Fragments arranged by size:
2678 1068 459
EarI CTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 942 1030 2413 2723 2869 4015 942
Size: 88 1383 310 146 1146 1132
Fragments arranged by size:
1383 1146 1132 310 146 88
EcoICRI GAG'CTC
Cuts at: 545 1217 545
Size: 672 3533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 2030 2462 3209 3477 191
Size: 1839 432 747 268 919
Fragments arranged by size:
1839 919 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1072 1072
Size: 4205
EcoO109I rG'GnC_Cy
Cuts at: 218 600 1344 218
Size: 382 744 3079
Fragments arranged by size:
3079 744 382
EcoRI G'AATT_C
Cuts at: 1209 1209
Size: 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 4205
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 208 561 590 704 831 934 1639 1887 2909
Size: 353 29 114 127 103 705 248 1022
Cuts at: 2909 3888 208
Size: 979 525
Fragments arranged by size:
1022 979 705 525 353 248 127 114
103 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2047 2077 2079 2109 2047
Size: 30 2 30 4143
Fragments arranged by size:
4143 30 30 2
HincII GTy'rAC
Cuts at: 471 909 918 1244 471
Size: 438 9 326 3432
Fragments arranged by size:
3432 438 326 9
KasI G'GCGC_C
Cuts at: 204 557 700 1635 1883 204
Size: 353 143 935 248 2526
Fragments arranged by size:
2526 935 353 248 143
KpnI G_GTAC'C
Cuts at: 662 1225 662
Size: 563 3642
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2876 3060 2876
Size: 184 4021
MscI TGG'CCA
Cuts at: 397 574 1966 397
Size: 177 1392 2636
Fragments arranged by size:
2636 1392 177
MslI CAynn'nnrTG
Cuts at: 295 508 724 781 2194 2321 295
Size: 213 216 57 1413 127 2179
Fragments arranged by size:
2179 1413 216 213 127 57
MspA1I CmG'CkG
Cuts at: 140 540 554 592 674 747 998 1316 1630
Size: 400 14 38 82 73 251 318 314
Cuts at: 1630 1990 3003 3248 140
Size: 360 1013 245 1097
Fragments arranged by size:
1097 1013 400 360 318 314 251 245
82 73 38 14
NaeI GCC'GGC
Cuts at: 203 957 2386 203
Size: 754 1429 2022
Fragments arranged by size:
2022 1429 754
NarI GG'CG_CC
Cuts at: 205 558 701 1636 1884 205
Size: 353 143 935 248 2526
Fragments arranged by size:
2526 935 353 248 143
NcoI C'CATG_G
Cuts at: 107 107
Size: 4205
NgoMIV G'CCGG_C
Cuts at: 201 955 2384 201
Size: 754 1429 2022
Fragments arranged by size:
2022 1429 754
NspI r_CATG'y
Cuts at: 378 618 1258 1758 2665 378
Size: 240 640 500 907 1918
Fragments arranged by size:
1918 907 640 500 240
PciI A'CATG_T
Cuts at: 2661 2661
Size: 4205
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 103 135 136 168 3344 3376 103
Size: 32 1 32 3176 32 932
Fragments arranged by size:
3176 932 32 32 32 1
PpuMI rG'GwC_Cy
Cuts at: 600 600
Size: 4205
PspOMI G'GGCC_C
Cuts at: 218 218
Size: 4205
PstI C_TGCA'G
Cuts at: 1252 1937 1252
Size: 685 3520
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 140 674 998 1630 1990 140
Size: 534 324 632 360 2355
Fragments arranged by size:
2355 632 534 360 324
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 547 1219 547
Size: 672 3533
SacII CC_GC'GG
Cuts at: 593 593
Size: 4205
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SfcI C'TryA_G
Cuts at: 33 1248 1933 2926 3117 33
Size: 1215 685 993 191 1121
Fragments arranged by size:
1215 1121 993 685 191
SfoI GGC'GCC
Cuts at: 206 559 702 1637 1885 206
Size: 353 143 935 248 2526
Fragments arranged by size:
2526 935 353 248 143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 158 223 1227 158
Size: 65 1004 3136
Fragments arranged by size:
3136 1004 65
SmlI C'TyrA_G
Cuts at: 1360 2275 2767 3029 1360
Size: 915 492 262 2536
Fragments arranged by size:
2536 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 1258 1758 1258
Size: 500 3705
SrfI GCCC'GGGC
Cuts at: 223 223
Size: 4205
StyI C'CwwG_G
Cuts at: 107 1088 1339 1693 107
Size: 981 251 354 2619
Fragments arranged by size:
2619 981 354 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1788 1921 2380 2589 1788
Size: 133 459 209 3404
Fragments arranged by size:
3404 459 209 133
TatI w'GTAC_w
Cuts at: 371 3720 371
Size: 3349 856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4173
Tth111I GACn'n_nGTC
Cuts at: 466 2002 466
Size: 1536 2669
XbaI T'CTAG_A
Cuts at: 1236 1236
Size: 4205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1700 1700
Size: 4205
XmaI C'CCGG_G
Cuts at: 156 221 1225 156
Size: 65 1004 3136
Fragments arranged by size:
3136 1004 65
ZraI GAC'GTC
Cuts at: 366 366
Size: 4205
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI