Restriction map for
pF1KB7245
(Circular) (Six-base) MAPSORT of: pF1KB7245.seq Check: 3646 from: 1 to: 4034
pF1KB7245 4034 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 832 832
Size: 4034
AccI GT'mk_AC
Cuts at: 209 1072 209
Size: 863 3171
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 3874 3874
Size: 4034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 1859 2291 3038 3306 314
Size: 1545 432 747 268 1042
Fragments arranged by size:
1545 1042 747 432 268
AflIII A'CryG_T
Cuts at: 1386 2490 3467 1386
Size: 1104 977 1953
Fragments arranged by size:
1953 1104 977
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AleI CACnn'nnGTG
Cuts at: 553 553
Size: 4034
AlwNI CAG_nnn'CTG
Cuts at: 337 1339 2906 337
Size: 1002 1567 1465
Fragments arranged by size:
1567 1465 1002
ApaLI G'TGCA_C
Cuts at: 2804 2804
Size: 4034
ApoI r'AATT_y
Cuts at: 1038 3387 3643 1038
Size: 2349 256 1429
Fragments arranged by size:
2349 1429 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 167 1054 167
Size: 887 3147
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 116 149 116
Size: 33 4001
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 433 1050 1464 1712 1747 433
Size: 617 414 248 35 2720
Fragments arranged by size:
2720 617 414 248 35
BanII G_rGCy'C
Cuts at: 158 176 1048 2078 158
Size: 18 872 1030 2114
Fragments arranged by size:
2114 1030 872 18
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BbsI GAAGACnn'nnnn_
Cuts at: 138 435 138
Size: 297 3737
BbvCI CC'TCA_GC
Cuts at: 781 781
Size: 4034
BciVI GTATCCnnnnn_n'
Cuts at: 1932 2693 4034 1932
Size: 761 1341 1932
Fragments arranged by size:
1932 1341 761
BclI T'GATC_A
Cuts at: 1554 1554
Size: 4034
BglII A'GATC_T
Cuts at: 407 704 1549 3586 407
Size: 297 845 2037 855
Fragments arranged by size:
2037 855 845 297
BlpI GC'TnA_GC
Cuts at: 1146 1146
Size: 4034
Bme1580I G_kGCm'C
Cuts at: 1659 1752 2808 1659
Size: 93 1056 2885
Fragments arranged by size:
2885 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1246 1327 1662 3806 1246
Size: 81 335 2144 1474
Fragments arranged by size:
2144 1474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 712 160
Size: 552 3482
Bpu10I CC'TnA_GC
Cuts at: 781 781
Size: 4034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1210 2089 2581 2879 685
Size: 525 879 492 298 1840
Fragments arranged by size:
1840 879 525 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3738 3738
Size: 4034
BsaAI yAC'GTr
Cuts at: 128 1387 128
Size: 1259 2775
BsaBI GATnn'nnATC
Cuts at: 6 1548 3585 6
Size: 1542 2037 455
Fragments arranged by size:
2037 1542 455
BsaHI Gr'CG_yC
Cuts at: 1465 1713 2415 3911 1465
Size: 248 702 1496 1588
Fragments arranged by size:
1588 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1223 1744 2422 2696 2843 3254 1223
Size: 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 551 581 551
Size: 30 4004
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 736 352
Size: 384 3650
BseYI C'CCAG_C
Cuts at: 266 1459 2217 2794 3290 266
Size: 1193 758 577 496 1010
Fragments arranged by size:
1193 1010 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 657 810 657
Size: 153 3881
BsiEI CG_ry'CG
Cuts at: 104 1576 1622 2830 104
Size: 1472 46 1208 1308
Fragments arranged by size:
1472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 176 1048 1826 2016 2808 176
Size: 872 778 190 792 1402
Fragments arranged by size:
1402 872 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 158 176 1048 1659 1752 1826 2016 2078 2808
Size: 18 872 611 93 74 190 62 730
Cuts at: 2808 158
Size: 1384
Fragments arranged by size:
1384 872 730 611 190 93 74 62
18
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 512 938 3210 512
Size: 426 2272 1336
Fragments arranged by size:
2272 1336 426
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1084 1298 1600 1981 832
Size: 252 214 302 381 2885
Fragments arranged by size:
2885 381 302 252 214
BsrBI CCG'CTC
Cuts at: 50 2326 2380 3864 4029 50
Size: 2276 54 1484 165 55
Fragments arranged by size:
2276 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 476 1946 476
Size: 1470 2564
BsrFI r'CCGG_y
Cuts at: 1019 2032 2213 2422 1019
Size: 1013 181 209 2631
Fragments arranged by size:
2631 1013 209 181
BssSI C'ACGA_G
Cuts at: 2305 2663 2305
Size: 358 3676
BstAPI GCAn_nnn'nTGC
Cuts at: 1411 1411
Size: 4034
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstXI CCAn_nnnn'nTGG
Cuts at: 225 555 627 225
Size: 330 72 3632
Fragments arranged by size:
3632 330 72
BstYI r'GATC_y
Cuts at: 1 407 704 1059 1526 1549 1884 2130 3142
Size: 406 297 355 467 23 335 246 1012
Cuts at: 3142 3228 3240 3586 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 406 355 346 335 297
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 973 973
Size: 4034
BtgI C'CryG_G
Cuts at: 107 437 107
Size: 330 3704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1984 2165 2328 1984
Size: 181 163 3690
Fragments arranged by size:
3690 181 163
BtsI GCAGTG_nn'
Cuts at: 335 601 685 1004 1277 335
Size: 266 84 319 273 3092
Fragments arranged by size:
3092 319 273 266 84
DraI TTT'AAA
Cuts at: 1033 1033
Size: 4034
DrdI GACnn_nn'nnGTC
Cuts at: 1740 2598 1740
Size: 858 3176
EaeI y'GGCC_r
Cuts at: 927 1619 1793 2184 2211 3556 927
Size: 692 174 391 27 1345 1405
Fragments arranged by size:
1405 1345 692 391 174 27
EagI C'GGCC_G
Cuts at: 1619 1619
Size: 4034
EarI CTCTTCn'nnn_
Cuts at: 333 2057 2267 333
Size: 1724 210 2100
Fragments arranged by size:
2100 1724 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2242 2552 2698 3844 2242
Size: 310 146 1146 2432
Fragments arranged by size:
2432 1146 310 146
EcoICRI GAG'CTC
Cuts at: 174 1046 174
Size: 872 3162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 314 712 1859 2291 3038 3306 160
Size: 154 398 1147 432 747 268 888
Fragments arranged by size:
1147 888 747 432 398 268 154
EcoO109I rG'GnC_Cy
Cuts at: 1173 1173
Size: 4034
EcoRI G'AATT_C
Cuts at: 1038 1038
Size: 4034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 4034
FspI TGC'GCA
Cuts at: 1815 1815
Size: 4034
HaeII r_GCGC'y
Cuts at: 1468 1716 2738 3717 1468
Size: 248 1022 979 1785
Fragments arranged by size:
1785 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 551 583 774 806 1876 1906 1908
Size: 32 170 32 191 32 1070 30 2
Cuts at: 1908 1938 349
Size: 30 2445
Fragments arranged by size:
2445 1070 191 170 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1073 1073
Size: 4034
HindIII A'AGCT_T
Cuts at: 913 913
Size: 4034
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 734 2705 2889 734
Size: 1971 184 1879
Fragments arranged by size:
1971 1879 184
MscI TGG'CCA
Cuts at: 1795 1795
Size: 4034
MslI CAynn'nnrTG
Cuts at: 223 250 280 511 553 772 841 937 2023
Size: 27 30 231 42 219 69 96 1086
Cuts at: 2023 2150 223
Size: 127 2107
Fragments arranged by size:
2107 1086 231 219 127 96 69 42
30 27
MspA1I CmG'CkG
Cuts at: 585 1145 1459 1819 2832 3077 585
Size: 560 314 360 1013 245 1542
Fragments arranged by size:
1542 1013 560 360 314 245
NaeI GCC'GGC
Cuts at: 2215 2215
Size: 4034
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NcoI C'CATG_G
Cuts at: 107 437 107
Size: 330 3704
NgoMIV G'CCGG_C
Cuts at: 2213 2213
Size: 4034
NspI r_CATG'y
Cuts at: 990 1087 1587 2494 990
Size: 97 500 907 2530
Fragments arranged by size:
2530 907 500 97
PciI A'CATG_T
Cuts at: 2490 2490
Size: 4034
PflMI CCAn_nnn'nTGG
Cuts at: 426 426
Size: 4034
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PmlI CAC'GTG
Cuts at: 128 128
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PstI C_TGCA'G
Cuts at: 1081 1766 1081
Size: 685 3349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 585 1459 1819 585
Size: 874 360 2800
Fragments arranged by size:
2800 874 360
RsrII CG'GwC_CG
Cuts at: 2229 2229
Size: 4034
SacI G_AGCT'C
Cuts at: 176 1048 176
Size: 872 3162
SalI G'TCGA_C
Cuts at: 1071 1071
Size: 4034
SapI GCTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 659 3551 659
Size: 2892 1142
SfcI C'TryA_G
Cuts at: 33 1077 1762 2755 2946 33
Size: 1044 685 993 191 1121
Fragments arranged by size:
1121 1044 993 685 191
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SmaI CCC'GGG
Cuts at: 1056 1056
Size: 4034
SmlI C'TyrA_G
Cuts at: 700 1189 2104 2596 2858 700
Size: 489 915 492 262 1876
Fragments arranged by size:
1876 915 492 489 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
StyI C'CwwG_G
Cuts at: 107 437 1168 1522 107
Size: 330 731 354 2619
Fragments arranged by size:
2619 731 354 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 519 543 847 1617 1750 2209 2418 294
Size: 225 24 304 770 133 459 209 1910
Fragments arranged by size:
1910 770 459 304 225 209 133 24
TatI w'GTAC_w
Cuts at: 284 657 3549 284
Size: 373 2892 769
Fragments arranged by size:
2892 769 373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4002
Tth111I GACn'n_nGTC
Cuts at: 142 1831 142
Size: 1689 2345
XbaI T'CTAG_A
Cuts at: 1065 1065
Size: 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 351 1529 351
Size: 1178 2856
XmaI C'CCGG_G
Cuts at: 1054 1054
Size: 4034
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI