Restriction map for
pF1KB7248
(Circular) (Six-base) MAPSORT of: pF1KB7248.seq Check: 6124 from: 1 to: 4715
pF1KB7248 4715 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1632 1632
Size: 4715
AccI GT'mk_AC
Cuts at: 1753 1753
Size: 4715
Acc65I G'GTAC_C
Cuts at: 1731 1731
Size: 4715
AclI AA'CG_TT
Cuts at: 4555 4555
Size: 4715
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 2540 2972 3719 3987 424
Size: 2116 432 747 268 1152
Fragments arranged by size:
2116 1152 747 432 268
AfeI AGC'GCT
Cuts at: 869 869
Size: 4715
AflIII A'CryG_T
Cuts at: 1034 1265 2067 3171 4148 1034
Size: 231 802 1104 977 1601
Fragments arranged by size:
1601 1104 977 802 231
AgeI A'CCGG_T
Cuts at: 3103 3103
Size: 4715
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 907 939 907
Size: 32 4683
AlwNI CAG_nnn'CTG
Cuts at: 474 752 1678 2020 3587 474
Size: 278 926 342 1567 1602
Fragments arranged by size:
1602 1567 926 342 278
ApaLI G'TGCA_C
Cuts at: 205 3485 205
Size: 3280 1435
ApoI r'AATT_y
Cuts at: 1105 1719 4068 4324 1105
Size: 614 2349 256 1496
Fragments arranged by size:
2349 1496 614 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4715
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
AvaI C'yCGr_G
Cuts at: 131 186 358 1129 1735 131
Size: 55 172 771 606 3111
Fragments arranged by size:
3111 771 606 172 55
BamHI G'GATC_C
Cuts at: 1740 1740
Size: 4715
BanI G'GyrC_C
Cuts at: 180 1731 2145 2393 2428 180
Size: 1551 414 248 35 2467
Fragments arranged by size:
2467 1551 414 248 35
BanII G_rGCy'C
Cuts at: 343 1469 1729 2759 343
Size: 1126 260 1030 2299
Fragments arranged by size:
2299 1126 1030 260
BbeI G_GCGC'C
Cuts at: 184 2149 2397 184
Size: 1965 248 2502
Fragments arranged by size:
2502 1965 248
BbsI GAAGACnn'nnnn_
Cuts at: 1268 1268
Size: 4715
BbvCI CC'TCA_GC
Cuts at: 220 220
Size: 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1591 1625 1591
Size: 34 4681
BciVI GTATCCnnnnn_n'
Cuts at: 2613 3374 4715 2613
Size: 761 1341 2613
Fragments arranged by size:
2613 1341 761
BclI T'GATC_A
Cuts at: 2235 2235
Size: 4715
BglI GCCn_nnn'nGGC
Cuts at: 189 244 189
Size: 55 4660
BglII A'GATC_T
Cuts at: 1158 2230 4267 1158
Size: 1072 2037 1606
Fragments arranged by size:
2037 1606 1072
BlpI GC'TnA_GC
Cuts at: 1827 1827
Size: 4715
Bme1580I G_kGCm'C
Cuts at: 209 2340 2433 3489 209
Size: 2131 93 1056 1435
Fragments arranged by size:
2131 1435 1056 93
BmgBI CAC'GTC
Cuts at: 851 851
Size: 4715
BmrI ACTGGGnnnn_n'
Cuts at: 1927 2008 2343 4487 1927
Size: 81 335 2144 2155
Fragments arranged by size:
2155 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3082 3082
Size: 4715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 809 884 132
Size: 677 75 3963
Fragments arranged by size:
3963 677 75
Bpu10I CC'TnA_GC
Cuts at: 220 220
Size: 4715
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1426 1891 2770 3262 3560 1426
Size: 465 879 492 298 2581
Fragments arranged by size:
2581 879 492 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 1609 4419 1609
Size: 2810 1905
BsaAI yAC'GTr
Cuts at: 2068 2068
Size: 4715
BsaBI GATnn'nnATC
Cuts at: 6 2229 4266 6
Size: 2223 2037 455
Fragments arranged by size:
2223 2037 455
BsaHI Gr'CG_yC
Cuts at: 181 527 2146 2394 3096 4592 181
Size: 346 1619 248 702 1496 304
Fragments arranged by size:
1619 1496 702 346 304 248
BsaWI w'CCGG_w
Cuts at: 1904 2425 3103 3377 3524 3935 1904
Size: 521 678 274 147 411 2684
Fragments arranged by size:
2684 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 782 812 782
Size: 30 4685
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 964 967 1211 1519 964
Size: 3 244 308 4160
Fragments arranged by size:
4160 308 244 3
BseYI C'CCAG_C
Cuts at: 1678 2140 2898 3475 3971 1678
Size: 462 758 577 496 2422
Fragments arranged by size:
2422 758 577 496 462
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1631 1631
Size: 4715
BsiEI CG_ry'CG
Cuts at: 104 1348 2257 2303 3511 104
Size: 1244 909 46 1208 1308
Fragments arranged by size:
1308 1244 1208 909 46
BsiHKAI G_wGCw'C
Cuts at: 209 1469 1729 2507 2697 3489 209
Size: 1260 260 778 190 792 1435
Fragments arranged by size:
1435 1260 792 778 260 190
BsmI GAATG_Cn'
Cuts at: 778 778
Size: 4715
BsmBI CGTCTCn'nnnn_
Cuts at: 675 1084 675
Size: 409 4306
Bsp1286I G_dGCh'C
Cuts at: 209 343 1469 1729 2340 2433 2507 2697 2759
Size: 134 1126 260 611 93 74 190 62
Cuts at: 2759 3489 209
Size: 730 1435
Fragments arranged by size:
1435 1126 730 611 260 190 134 93
74 62
BspEI T'CCGG_A
Cuts at: 3935 3935
Size: 4715
BspHI T'CATG_A
Cuts at: 251 3891 251
Size: 3640 1075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 760 1316 1632 1765 1979 2281 2662 760
Size: 556 316 133 214 302 381 2813
Fragments arranged by size:
2813 556 381 316 302 214 133
BsrBI CCG'CTC
Cuts at: 50 1289 3007 3061 4545 4710 50
Size: 1239 1718 54 1484 165 55
Fragments arranged by size:
1718 1484 1239 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2627 2627
Size: 4715
BsrFI r'CCGG_y
Cuts at: 433 1343 1347 2713 2894 3103 433
Size: 910 4 1366 181 209 2045
Fragments arranged by size:
2045 1366 910 209 181 4
BsrGI T'GTAC_A
Cuts at: 739 935 739
Size: 196 4519
BssSI C'ACGA_G
Cuts at: 208 1117 2986 3344 208
Size: 909 1869 358 1579
Fragments arranged by size:
1869 1579 909 358
BstAPI GCAn_nnn'nTGC
Cuts at: 213 474 2092 213
Size: 261 1618 2836
Fragments arranged by size:
2836 1618 261
BstBI TT'CG_AA
Cuts at: 1601 3076 1601
Size: 1475 3240
BstEII G'GTnAC_C
Cuts at: 1423 1423
Size: 4715
BstXI CCAn_nnnn'nTGG
Cuts at: 1374 1374
Size: 4715
BstYI r'GATC_y
Cuts at: 1 1158 1740 2207 2230 2565 2811 3823 3909
Size: 1157 582 467 23 335 246 1012 86
Cuts at: 3909 3921 4267 1
Size: 12 346 449
Fragments arranged by size:
1157 1012 582 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2665 2846 3009 2665
Size: 181 163 4371
Fragments arranged by size:
4371 181 163
BtsI GCAGTG_nn'
Cuts at: 1958 1958
Size: 4715
DraI TTT'AAA
Cuts at: 1714 1714
Size: 4715
DraIII CAC_nnn'GTG
Cuts at: 425 450 893 425
Size: 25 443 4247
Fragments arranged by size:
4247 443 25
DrdI GACnn_nn'nnGTC
Cuts at: 1076 1411 2421 3279 1076
Size: 335 1010 858 2512
Fragments arranged by size:
2512 1010 858 335
EaeI y'GGCC_r
Cuts at: 452 488 706 1001 1345 2300 2474 2865 2892
Size: 36 218 295 344 955 174 391 27
Cuts at: 2892 4237 452
Size: 1345 930
Fragments arranged by size:
1345 955 930 391 344 295 218 174
36 27
EagI C'GGCC_G
Cuts at: 1345 2300 1345
Size: 955 3760
EarI CTCTTCn'nnn_
Cuts at: 271 952 2738 2948 271
Size: 681 1786 210 2038
Fragments arranged by size:
2038 1786 681 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 207 1078 1698 2923 3233 3379 4525 207
Size: 871 620 1225 310 146 1146 397
Fragments arranged by size:
1225 1146 871 620 397 310 146
EcoICRI GAG'CTC
Cuts at: 1467 1727 1467
Size: 260 4455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 424 809 884 2540 2972 3719 3987 132
Size: 292 385 75 1656 432 747 268 860
Fragments arranged by size:
1656 860 747 432 385 292 268 75
EcoNI CCTnn'n_nnAGG
Cuts at: 232 403 232
Size: 171 4544
EcoO109I rG'GnC_Cy
Cuts at: 749 859 1125 1854 749
Size: 110 266 729 3610
Fragments arranged by size:
3610 729 266 110
EcoRI G'AATT_C
Cuts at: 1719 1719
Size: 4715
EcoRV GAT'ATC
Cuts at: 820 820
Size: 4715
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4715
FseI GG_CCGG'CC
Cuts at: 1351 1351
Size: 4715
FspI TGC'GCA
Cuts at: 418 2496 418
Size: 2078 2637
FspAI rTGC'GCAy
Cuts at: 418 418
Size: 4715
HaeII r_GCGC'y
Cuts at: 184 227 475 796 871 2149 2397 3419 4398
Size: 43 248 321 75 1278 248 1022 979
Cuts at: 4398 184
Size: 501
Fragments arranged by size:
1278 1022 979 501 321 248 248 75
43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 782 814 1156 1188 2557 2587 2589 2619 782
Size: 32 342 32 1369 30 2 30 2878
Fragments arranged by size:
2878 1369 342 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1754 1754
Size: 4715
HindIII A'AGCT_T
Cuts at: 1147 1147
Size: 4715
KasI G'GCGC_C
Cuts at: 180 2145 2393 180
Size: 1965 248 2502
Fragments arranged by size:
2502 1965 248
KpnI G_GTAC'C
Cuts at: 1735 1735
Size: 4715
MluI A'CGCG_T
Cuts at: 4148 4148
Size: 4715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 719 3386 3570 719
Size: 2667 184 1864
Fragments arranged by size:
2667 1864 184
MscI TGG'CCA
Cuts at: 708 2476 708
Size: 1768 2947
MslI CAynn'nnrTG
Cuts at: 535 547 562 849 2704 2831 535
Size: 12 15 287 1855 127 2419
Fragments arranged by size:
2419 1855 287 127 15 12
MspA1I CmG'CkG
Cuts at: 292 1053 1087 1295 1374 1826 2140 2500 3513
Size: 761 34 208 79 452 314 360 1013
Cuts at: 3513 3758 292
Size: 245 1249
Fragments arranged by size:
1249 1013 761 452 360 314 245 208
79 34
NaeI GCC'GGC
Cuts at: 435 1345 1349 2896 435
Size: 910 4 1547 2254
Fragments arranged by size:
2254 1547 910 4
NarI GG'CG_CC
Cuts at: 181 2146 2394 181
Size: 1965 248 2502
Fragments arranged by size:
2502 1965 248
NgoMIV G'CCGG_C
Cuts at: 433 1343 1347 2894 433
Size: 910 4 1547 2254
Fragments arranged by size:
2254 1547 910 4
NspI r_CATG'y
Cuts at: 1269 1768 2268 3175 1269
Size: 499 500 907 2809
Fragments arranged by size:
2809 907 500 499
PasI CC'CwG_GG
Cuts at: 336 1452 336
Size: 1116 3599
PciI A'CATG_T
Cuts at: 1265 3171 1265
Size: 1906 2809
PflMI CCAn_nnn'nTGG
Cuts at: 450 807 845 450
Size: 357 38 4320
Fragments arranged by size:
4320 357 38
PmeI GTTT'AAAC
Cuts at: 1714 1714
Size: 4715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 3854
Size: 32 4683
PpuMI rG'GwC_Cy
Cuts at: 749 859 749
Size: 110 4605
PstI C_TGCA'G
Cuts at: 233 1762 2447 233
Size: 1529 685 2501
Fragments arranged by size:
2501 1529 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4715
PvuII CAG'CTG
Cuts at: 1053 1374 2140 2500 1053
Size: 321 766 360 3268
Fragments arranged by size:
3268 766 360 321
RsrII CG'GwC_CG
Cuts at: 273 2910 273
Size: 2637 2078
SacI G_AGCT'C
Cuts at: 1469 1729 1469
Size: 260 4455
SalI G'TCGA_C
Cuts at: 1752 1752
Size: 4715
SapI GCTCTTCn'nnn_
Cuts at: 271 2738 2948 271
Size: 2467 210 2038
Fragments arranged by size:
2467 2038 210
SbfI CC_TGCA'GG
Cuts at: 1762 1762
Size: 4715
ScaI AGT'ACT
Cuts at: 4232 4232
Size: 4715
SexAI A'CCwGG_T
Cuts at: 771 771
Size: 4715
SfcI C'TryA_G
Cuts at: 33 229 1758 2443 3436 3627 33
Size: 196 1529 685 993 191 1121
Fragments arranged by size:
1529 1121 993 685 196 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 244 244
Size: 4715
SfoI GGC'GCC
Cuts at: 182 2147 2395 182
Size: 1965 248 2502
Fragments arranged by size:
2502 1965 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
SgrAI Cr'CCGG_yG
Cuts at: 433 433
Size: 4715
SmaI CCC'GGG
Cuts at: 133 188 1737 133
Size: 55 1549 3111
Fragments arranged by size:
3111 1549 55
SmlI C'TyrA_G
Cuts at: 1441 1870 2785 3277 3539 1441
Size: 429 915 492 262 2617
Fragments arranged by size:
2617 915 492 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4715
SphI G_CATG'C
Cuts at: 1768 2268 1768
Size: 500 4215
StuI AGG'CCT
Cuts at: 1009 1009
Size: 4715
StyI C'CwwG_G
Cuts at: 1010 1849 2203 1010
Size: 839 354 3522
Fragments arranged by size:
3522 839 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1003 1492 2298 2431 2890 3099 1003
Size: 489 806 133 459 209 2619
Fragments arranged by size:
2619 806 489 459 209 133
TatI w'GTAC_w
Cuts at: 739 935 4230 739
Size: 196 3295 1224
Fragments arranged by size:
3295 1224 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 865 897 4232 4264 865
Size: 32 3335 32 1316
Fragments arranged by size:
3335 1316 32 32
Tth111I GACn'n_nGTC
Cuts at: 549 2512 549
Size: 1963 2752
XbaI T'CTAG_A
Cuts at: 1746 1746
Size: 4715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 303 2210 303
Size: 1907 2808
XmaI C'CCGG_G
Cuts at: 131 186 1735 131
Size: 55 1549 3111
Fragments arranged by size:
3111 1549 55
XmnI GAAnn'nnTTC
Cuts at: 199 199
Size: 4715
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI ApaI AscI AvrII
BaeI BfrBI BmtI BsiWI BssHII BstZ17I Bsu36I BtgI
ClaI CspCI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SnaBI SrfI SspI SwaI
XhoI ZraI