Restriction map for
pF1KB7253
(Circular) (Six-base) MAPSORT of: pF1KB7253.seq Check: 6511 from: 1 to: 4370
pF1KB7253 4370 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1408 1408
Size: 4370
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 2195 2627 3374 3642 1004
Size: 1191 432 747 268 1732
Fragments arranged by size:
1732 1191 747 432 268
AflIII A'CryG_T
Cuts at: 1722 2826 3803 1722
Size: 1104 977 2289
Fragments arranged by size:
2289 1104 977
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1071 1071
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 1675 3242 1675
Size: 1567 2803
ApaLI G'TGCA_C
Cuts at: 3140 3140
Size: 4370
ApoI r'AATT_y
Cuts at: 654 665 738 861 1139 1374 3723 3979 654
Size: 11 73 123 278 235 2349 256 1045
Fragments arranged by size:
2349 1045 278 256 235 123 73 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 1390 1390
Size: 4370
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 1386 1800 2048 2083 1386
Size: 414 248 35 3673
Fragments arranged by size:
3673 414 248 35
BanII G_rGCy'C
Cuts at: 1384 2414 1384
Size: 1030 3340
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BbsI GAAGACnn'nnnn_
Cuts at: 443 1106 443
Size: 663 3707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4336
BciVI GTATCCnnnnn_n'
Cuts at: 1139 2268 3029 4370 1139
Size: 1129 761 1341 1139
Fragments arranged by size:
1341 1139 1129 761
BclI T'GATC_A
Cuts at: 1164 1890 1164
Size: 726 3644
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4370
Bme1580I G_kGCm'C
Cuts at: 1995 2088 3144 1995
Size: 93 1056 3221
Fragments arranged by size:
3221 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1117 1582 1663 1998 4142 1117
Size: 465 81 335 2144 1345
Fragments arranged by size:
2144 1345 465 335 81
BmtI G_CTAG'C
Cuts at: 686 686
Size: 4370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 110
Size: 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1546 2425 2917 3215 818
Size: 728 879 492 298 1973
Fragments arranged by size:
1973 879 728 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4074 4074
Size: 4370
BsaAI yAC'GTr
Cuts at: 1723 1723
Size: 4370
BsaBI GATnn'nnATC
Cuts at: 6 1884 3921 6
Size: 1878 2037 455
Fragments arranged by size:
2037 1878 455
BsaHI Gr'CG_yC
Cuts at: 1801 2049 2751 4247 1801
Size: 248 702 1496 1924
Fragments arranged by size:
1924 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3032 3179 3590 1559
Size: 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1795 2553 3130 3626 1795
Size: 758 577 496 2539
Fragments arranged by size:
2539 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1041 1064 1041
Size: 23 4347
BsiEI CG_ry'CG
Cuts at: 104 1912 1958 3166 104
Size: 1808 46 1208 1308
Fragments arranged by size:
1808 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1384 2162 2352 3144 1384
Size: 778 190 792 2610
Fragments arranged by size:
2610 792 778 190
BsmI GAATG_Cn'
Cuts at: 428 684 428
Size: 256 4114
Bsp1286I G_dGCh'C
Cuts at: 1384 1995 2088 2162 2352 2414 3144 1384
Size: 611 93 74 190 62 730 2610
Fragments arranged by size:
2610 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3590 3590
Size: 4370
BspHI T'CATG_A
Cuts at: 3546 3546
Size: 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1420 1634 1936 2317 1420
Size: 214 302 381 3473
Fragments arranged by size:
3473 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2662 2716 4200 4365 50
Size: 2612 54 1484 165 55
Fragments arranged by size:
2612 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2282 2282
Size: 4370
BsrFI r'CCGG_y
Cuts at: 2368 2549 2758 2368
Size: 181 209 3980
Fragments arranged by size:
3980 209 181
BsrGI T'GTAC_A
Cuts at: 195 1144 195
Size: 949 3421
BssSI C'ACGA_G
Cuts at: 2641 2999 2641
Size: 358 4012
BstAPI GCAn_nnn'nTGC
Cuts at: 1051 1747 1051
Size: 696 3674
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstYI r'GATC_y
Cuts at: 1 1214 1395 1862 1885 2220 2466 3478 3564
Size: 1213 181 467 23 335 246 1012 86
Cuts at: 3564 3576 3922 1
Size: 12 346 449
Fragments arranged by size:
1213 1012 467 449 346 335 246 181
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 1355 1362 1613 1355
Size: 7 251 4112
Fragments arranged by size:
4112 251 7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 866 901 866
Size: 35 4335
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 1306 2076 2934 1306
Size: 770 858 2742
Fragments arranged by size:
2742 858 770
EaeI y'GGCC_r
Cuts at: 1104 1955 2129 2520 2547 3892 1104
Size: 851 174 391 27 1345 1582
Fragments arranged by size:
1582 1345 851 391 174 27
EagI C'GGCC_G
Cuts at: 1955 1955
Size: 4370
EarI CTCTTCn'nnn_
Cuts at: 1091 2393 2603 1091
Size: 1302 210 2858
Fragments arranged by size:
2858 1302 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 2888 3034 4180 2578
Size: 310 146 1146 2768
Fragments arranged by size:
2768 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1382 1382
Size: 4370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 1004 2195 2627 3374 3642 110
Size: 894 1191 432 747 268 838
Fragments arranged by size:
1191 894 838 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 244 1509 244
Size: 1265 3105
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 467 2389 467
Size: 1922 2448
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 1804 2052 3074 4053 1804
Size: 248 1022 979 2121
Fragments arranged by size:
2121 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 694 726 2212 2242 2244 2274 694
Size: 32 1486 30 2 30 2790
Fragments arranged by size:
2790 1486 32 30 30 2
HincII GTy'rAC
Cuts at: 615 1409 615
Size: 794 3576
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3041 3225 3041
Size: 184 4186
MscI TGG'CCA
Cuts at: 1106 2131 1106
Size: 1025 3345
MslI CAynn'nnrTG
Cuts at: 158 2359 2486 158
Size: 2201 127 2042
Fragments arranged by size:
2201 2042 127
MspA1I CmG'CkG
Cuts at: 1481 1795 2155 3168 3413 1481
Size: 314 360 1013 245 2438
Fragments arranged by size:
2438 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4370
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4370
NheI G'CTAG_C
Cuts at: 682 682
Size: 4370
NspI r_CATG'y
Cuts at: 621 1423 1923 2830 621
Size: 802 500 907 2161
Fragments arranged by size:
2161 907 802 500
PciI A'CATG_T
Cuts at: 2826 2826
Size: 4370
PflMI CCAn_nnn'nTGG
Cuts at: 306 379 463 306
Size: 73 84 4213
Fragments arranged by size:
4213 84 73
PfoI T'CCnGG_A
Cuts at: 723 723
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 4338
PpuMI rG'GwC_Cy
Cuts at: 244 244
Size: 4370
PsiI TTA'TAA
Cuts at: 590 590
Size: 4370
PstI C_TGCA'G
Cuts at: 1260 1272 1350 1417 2102 1260
Size: 12 78 67 685 3528
Fragments arranged by size:
3528 685 78 67 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 1795 2155 1795
Size: 360 4010
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1384 1384
Size: 4370
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SexAI A'CCwGG_T
Cuts at: 189 189
Size: 4370
SfcI C'TryA_G
Cuts at: 33 408 1256 1268 1346 1413 2098 3091 3282
Size: 375 848 12 78 67 685 993 191
Cuts at: 3282 33
Size: 1121
Fragments arranged by size:
1121 993 848 685 375 191 78 67
12
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 833 1525 2440 2932 3194 833
Size: 692 915 492 262 2009
Fragments arranged by size:
2009 915 692 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
SspI AAT'ATT
Cuts at: 918 918
Size: 4370
StuI AGG'CCT
Cuts at: 1189 1189
Size: 4370
StyI C'CwwG_G
Cuts at: 1504 1858 1504
Size: 354 4016
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 509 1106 1953 2086 2545 2754 509
Size: 597 847 133 459 209 2125
Fragments arranged by size:
2125 847 597 459 209 133
TatI w'GTAC_w
Cuts at: 195 412 1144 1219 3885 195
Size: 217 732 75 2666 680
Fragments arranged by size:
2666 732 680 217 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4338
Tth111I GACn'n_nGTC
Cuts at: 2167 2167
Size: 4370
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1865 1865
Size: 4370
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
XmnI GAAnn'nnTTC
Cuts at: 450 732 450
Size: 282 4088
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI Bpu10I
BsaXI BseRI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NotI NruI NsiI
PacI PasI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI