Restriction map for
pF1KB7254
(Circular) (Six-base) MAPSORT of: pF1KB7254.seq Check: 2075 from: 1 to: 5045
pF1KB7254 5045 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 303 303
Size: 5045
AatII G_ACGT'C
Cuts at: 1637 1637
Size: 5045
AccI GT'mk_AC
Cuts at: 1043 2083 1043
Size: 1040 4005
Acc65I G'GTAC_C
Cuts at: 2061 2061
Size: 5045
AclI AA'CG_TT
Cuts at: 4885 4885
Size: 5045
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2870 3302 4049 4317 2870
Size: 432 747 268 3598
Fragments arranged by size:
3598 747 432 268
AflII C'TTAA_G
Cuts at: 1000 1000
Size: 5045
AflIII A'CryG_T
Cuts at: 464 1754 2000 2397 3501 4478 464
Size: 1290 246 397 1104 977 1031
Fragments arranged by size:
1290 1104 1031 977 397 246
AgeI A'CCGG_T
Cuts at: 3433 3433
Size: 5045
AleI CACnn'nnGTG
Cuts at: 542 655 2011 542
Size: 113 1356 3576
Fragments arranged by size:
3576 1356 113
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1516 1548 1516
Size: 32 5013
AlwNI CAG_nnn'CTG
Cuts at: 977 1803 2350 3917 977
Size: 826 547 1567 2105
Fragments arranged by size:
2105 1567 826 547
ApaI G_GGCC'C
Cuts at: 1268 1268
Size: 5045
ApaLI G'TGCA_C
Cuts at: 3815 3815
Size: 5045
ApoI r'AATT_y
Cuts at: 388 1011 2049 4398 4654 388
Size: 623 1038 2349 256 779
Fragments arranged by size:
2349 1038 779 623 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5045
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5045
AvaI C'yCGr_G
Cuts at: 1431 2065 1431
Size: 634 4411
BamHI G'GATC_C
Cuts at: 1819 2070 1819
Size: 251 4794
BanI G'GyrC_C
Cuts at: 649 740 919 1946 2061 2475 2723 2758 649
Size: 91 179 1027 115 414 248 35 2936
Fragments arranged by size:
2936 1027 414 248 179 115 91 35
BanII G_rGCy'C
Cuts at: 664 707 1268 1794 2059 3089 664
Size: 43 561 526 265 1030 2620
Fragments arranged by size:
2620 1030 561 526 265 43
BbeI G_GCGC'C
Cuts at: 2479 2727 2479
Size: 248 4797
BbsI GAAGACnn'nnnn_
Cuts at: 284 429 612 284
Size: 145 183 4717
Fragments arranged by size:
4717 183 145
BbvCI CC'TCA_GC
Cuts at: 748 1322 748
Size: 574 4471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5011
BciVI GTATCCnnnnn_n'
Cuts at: 2943 3704 5045 2943
Size: 761 1341 2943
Fragments arranged by size:
2943 1341 761
BclI T'GATC_A
Cuts at: 1034 1160 2565 1034
Size: 126 1405 3514
Fragments arranged by size:
3514 1405 126
BglI GCCn_nnn'nGGC
Cuts at: 205 205
Size: 5045
BglII A'GATC_T
Cuts at: 521 2560 4597 521
Size: 2039 2037 969
Fragments arranged by size:
2039 2037 969
BlpI GC'TnA_GC
Cuts at: 178 663 2157 178
Size: 485 1494 3066
Fragments arranged by size:
3066 1494 485
Bme1580I G_kGCm'C
Cuts at: 1268 1278 2670 2763 3819 1268
Size: 10 1392 93 1056 2494
Fragments arranged by size:
2494 1392 1056 93 10
BmrI ACTGGGnnnn_n'
Cuts at: 2257 2338 2673 4817 2257
Size: 81 335 2144 2485
Fragments arranged by size:
2485 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3412 3412
Size: 5045
Bpu10I CC'TnA_GC
Cuts at: 748 1322 748
Size: 574 4471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 3100 3592 3890 2221
Size: 879 492 298 3376
Fragments arranged by size:
3376 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1786 4749 1786
Size: 2963 2082
BsaAI yAC'GTr
Cuts at: 2398 2398
Size: 5045
BsaBI GATnn'nnATC
Cuts at: 6 2559 4596 6
Size: 2553 2037 455
Fragments arranged by size:
2553 2037 455
BsaHI Gr'CG_yC
Cuts at: 1634 1901 2476 2724 3426 4922 1634
Size: 267 575 248 702 1496 1757
Fragments arranged by size:
1757 1496 702 575 267 248
BsaWI w'CCGG_w
Cuts at: 350 2234 2755 3433 3707 3854 4265 350
Size: 1884 521 678 274 147 411 1130
Fragments arranged by size:
1884 1130 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1516 1546 1516
Size: 30 5015
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 921 1387 1396 1942 921
Size: 466 9 546 4024
Fragments arranged by size:
4024 546 466 9
BseYI C'CCAG_C
Cuts at: 556 712 1267 1520 1663 1935 2470 3228 3805
Size: 156 555 253 143 272 535 758 577
Cuts at: 3805 4301 556
Size: 496 1300
Fragments arranged by size:
1300 758 577 555 535 496 272 253
156 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 520 351
Size: 169 4876
BsiEI CG_ry'CG
Cuts at: 104 115 2587 2633 3841 104
Size: 11 2472 46 1208 1308
Fragments arranged by size:
2472 1308 1208 46 11
BsiHKAI G_wGCw'C
Cuts at: 452 2059 2837 3027 3819 452
Size: 1607 778 190 792 1678
Fragments arranged by size:
1678 1607 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 452 664 707 1268 1278 1794 2059 2670 2763
Size: 212 43 561 10 516 265 611 93
Cuts at: 2763 2837 3027 3089 3819 452
Size: 74 190 62 730 1678
Fragments arranged by size:
1678 730 611 561 516 265 212 190
93 74 62 43 10
BspEI T'CCGG_A
Cuts at: 350 4265 350
Size: 3915 1130
BspHI T'CATG_A
Cuts at: 869 1103 1985 4221 869
Size: 234 882 2236 1693
Fragments arranged by size:
2236 1693 882 234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 303 708 2027 2095 2309 2611 2992 303
Size: 405 1319 68 214 302 381 2356
Fragments arranged by size:
2356 1319 405 381 302 214 68
BsrBI CCG'CTC
Cuts at: 50 3337 3391 4875 5040 50
Size: 3287 54 1484 165 55
Fragments arranged by size:
3287 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2957 2957
Size: 5045
BsrFI r'CCGG_y
Cuts at: 3043 3224 3433 3043
Size: 181 209 4655
Fragments arranged by size:
4655 209 181
BssHII G'CGCG_C
Cuts at: 134 134
Size: 5045
BssSI C'ACGA_G
Cuts at: 3316 3674 3316
Size: 358 4687
BstAPI GCAn_nnn'nTGC
Cuts at: 365 977 2422 365
Size: 612 1445 2988
Fragments arranged by size:
2988 1445 612
BstBI TT'CG_AA
Cuts at: 1098 3406 1098
Size: 2308 2737
BstXI CCAn_nnnn'nTGG
Cuts at: 570 570
Size: 5045
BstYI r'GATC_y
Cuts at: 1 521 1819 2070 2537 2560 2895 3141 4153
Size: 520 1298 251 467 23 335 246 1012
Cuts at: 4153 4239 4251 4597 1
Size: 86 12 346 449
Fragments arranged by size:
1298 1012 520 467 449 346 335 251
246 86 23 12
BtgI C'CryG_G
Cuts at: 1142 1247 1142
Size: 105 4940
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 650 2995 3176 3339 650
Size: 2345 181 163 2356
Fragments arranged by size:
2356 2345 181 163
BtsI GCAGTG_nn'
Cuts at: 1278 2288 1278
Size: 1010 4035
DraI TTT'AAA
Cuts at: 2044 2044
Size: 5045
DraIII CAC_nnn'GTG
Cuts at: 1248 1562 1248
Size: 314 4731
DrdI GACnn_nn'nnGTC
Cuts at: 502 2751 3609 502
Size: 2249 858 1938
Fragments arranged by size:
2249 1938 858
EaeI y'GGCC_r
Cuts at: 112 479 944 1145 1220 1468 1688 1703 2630
Size: 367 465 201 75 248 220 15 927
Cuts at: 2630 2804 3195 3222 4567 112
Size: 174 391 27 1345 590
Fragments arranged by size:
1345 927 590 465 391 367 248 220
201 174 75 27 15
EagI C'GGCC_G
Cuts at: 112 2630 112
Size: 2518 2527
EarI CTCTTCn'nnn_
Cuts at: 1373 3068 3278 1373
Size: 1695 210 3140
Fragments arranged by size:
3140 1695 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3253 3563 3709 4855 3253
Size: 310 146 1146 3443
Fragments arranged by size:
3443 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2057 2057
Size: 5045
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2870 3302 4049 4317 2870
Size: 432 747 268 3598
Fragments arranged by size:
3598 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 168 1264 2184 168
Size: 1096 920 3029
Fragments arranged by size:
3029 1096 920
EcoRI G'AATT_C
Cuts at: 388 2049 388
Size: 1661 3384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3064 3064
Size: 5045
FspI TGC'GCA
Cuts at: 2826 2826
Size: 5045
HaeII r_GCGC'y
Cuts at: 211 534 2479 2727 3749 4728 211
Size: 323 1945 248 1022 979 528
Fragments arranged by size:
1945 1022 979 528 323 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1039 1071 1414 1446 1804 1811 1836 1843 2887
Size: 32 343 32 358 7 25 7 1044
Cuts at: 2887 2917 2919 2949 1039
Size: 30 2 30 3135
Fragments arranged by size:
3135 1044 358 343 32 32 30 30
25 7 7 2
HincII GTy'rAC
Cuts at: 936 2084 936
Size: 1148 3897
KasI G'GCGC_C
Cuts at: 2475 2723 2475
Size: 248 4797
KpnI G_GTAC'C
Cuts at: 2065 2065
Size: 5045
MluI A'CGCG_T
Cuts at: 4478 4478
Size: 5045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1760 3716 3900 1760
Size: 1956 184 2905
Fragments arranged by size:
2905 1956 184
MscI TGG'CCA
Cuts at: 481 946 1147 1222 1690 1705 2806 481
Size: 465 201 75 468 15 1101 2720
Fragments arranged by size:
2720 1101 468 465 201 75 15
MslI CAynn'nnrTG
Cuts at: 463 542 568 655 1588 1729 2011 3034 3161
Size: 79 26 87 933 141 282 1023 127
Cuts at: 3161 463
Size: 2347
Fragments arranged by size:
2347 1023 933 282 141 127 87 79
26
MspA1I CmG'CkG
Cuts at: 577 667 1473 1923 2156 2470 2830 3843 4088
Size: 90 806 450 233 314 360 1013 245
Cuts at: 4088 577
Size: 1534
Fragments arranged by size:
1534 1013 806 450 360 314 245 233
90
NaeI GCC'GGC
Cuts at: 3226 3226
Size: 5045
NarI GG'CG_CC
Cuts at: 2476 2724 2476
Size: 248 4797
NcoI C'CATG_G
Cuts at: 1142 1142
Size: 5045
NgoMIV G'CCGG_C
Cuts at: 3224 3224
Size: 5045
NspI r_CATG'y
Cuts at: 468 1158 2098 2598 3505 468
Size: 690 940 500 907 2008
Fragments arranged by size:
2008 940 907 690 500
PasI CC'CwG_GG
Cuts at: 1620 1787 1620
Size: 167 4878
PciI A'CATG_T
Cuts at: 464 3501 464
Size: 3037 2008
PflMI CCAn_nnn'nTGG
Cuts at: 1562 1562
Size: 5045
PmeI GTTT'AAAC
Cuts at: 2044 2044
Size: 5045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1516 1548 4184 4216 1516
Size: 32 2636 32 2345
Fragments arranged by size:
2636 2345 32 32
PpuMI rG'GwC_Cy
Cuts at: 168 168
Size: 5045
PspOMI G'GGCC_C
Cuts at: 1264 1264
Size: 5045
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1951 1983 1951
Size: 32 5013
PstI C_TGCA'G
Cuts at: 1799 2092 2777 1799
Size: 293 685 4067
Fragments arranged by size:
4067 685 293
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5045
PvuII CAG'CTG
Cuts at: 1923 2470 2830 1923
Size: 547 360 4138
Fragments arranged by size:
4138 547 360
RsrII CG'GwC_CG
Cuts at: 3240 3240
Size: 5045
SacI G_AGCT'C
Cuts at: 2059 2059
Size: 5045
SalI G'TCGA_C
Cuts at: 2082 2082
Size: 5045
SapI GCTCTTCn'nnn_
Cuts at: 3068 3278 3068
Size: 210 4835
SbfI CC_TGCA'GG
Cuts at: 2092 2092
Size: 5045
ScaI AGT'ACT
Cuts at: 4562 4562
Size: 5045
SfcI C'TryA_G
Cuts at: 33 1795 2088 2773 3766 3957 33
Size: 1762 293 685 993 191 1121
Fragments arranged by size:
1762 1121 993 685 293 191
SfoI GGC'GCC
Cuts at: 2477 2725 2477
Size: 248 4797
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5045
SmaI CCC'GGG
Cuts at: 2067 2067
Size: 5045
SmlI C'TyrA_G
Cuts at: 1000 2200 3115 3607 3869 1000
Size: 1200 915 492 262 2176
Fragments arranged by size:
2176 1200 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5045
SphI G_CATG'C
Cuts at: 2098 2598 2098
Size: 500 4545
StyI C'CwwG_G
Cuts at: 512 1142 2179 2533 512
Size: 630 1037 354 3024
Fragments arranged by size:
3024 1037 630 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 597 1768 1934 2628 2761 3220 3429 597
Size: 1171 166 694 133 459 209 2213
Fragments arranged by size:
2213 1171 694 459 209 166 133
TatI w'GTAC_w
Cuts at: 4560 4560
Size: 5045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 903 935 1060 1092 4562 4594 903
Size: 32 125 32 3470 32 1354
Fragments arranged by size:
3470 1354 125 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2842 2842
Size: 5045
XbaI T'CTAG_A
Cuts at: 2076 2076
Size: 5045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 636 1698 1713 2540 636
Size: 1062 15 827 3141
Fragments arranged by size:
3141 1062 827 15
XmaI C'CCGG_G
Cuts at: 2065 2065
Size: 5045
XmnI GAAnn'nnTTC
Cuts at: 1531 1531
Size: 5045
ZraI GAC'GTC
Cuts at: 1635 1635
Size: 5045
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AscI AvrII BaeI BfrBI BmgBI
BmtI BpmI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI