Restriction map for
pF1KB7255
(Circular) (Six-base) MAPSORT of: pF1KB7255.seq Check: 40 from: 1 to: 4046
pF1KB7255 4046 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 835 835
Size: 4046
AccI GT'mk_AC
Cuts at: 314 1084 314
Size: 770 3276
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1030 1871 2303 3050 3318 805
Size: 225 841 432 747 268 1533
Fragments arranged by size:
1533 841 747 432 268 225
AflIII A'CryG_T
Cuts at: 1398 2502 3479 1398
Size: 1104 977 1965
Fragments arranged by size:
1965 1104 977
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AleI CACnn'nnGTG
Cuts at: 130 946 130
Size: 816 3230
AlwNI CAG_nnn'CTG
Cuts at: 1351 2918 1351
Size: 1567 2479
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 206 1050 3399 3655 206
Size: 844 2349 256 597
Fragments arranged by size:
2349 844 597 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 158 191 158
Size: 33 4013
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 1062 1476 1724 1759 1062
Size: 414 248 35 3349
Fragments arranged by size:
3349 414 248 35
BanII G_rGCy'C
Cuts at: 1008 1060 2090 1008
Size: 52 1030 2964
Fragments arranged by size:
2964 1030 52
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BciVI GTATCCnnnnn_n'
Cuts at: 159 1944 2705 4046 159
Size: 1785 761 1341 159
Fragments arranged by size:
1785 1341 761 159
BclI T'GATC_A
Cuts at: 251 1566 251
Size: 1315 2731
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 1158 1158
Size: 4046
Bme1580I G_kGCm'C
Cuts at: 1671 1764 2820 1671
Size: 93 1056 2897
Fragments arranged by size:
2897 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1258 1339 1674 3818 1258
Size: 81 335 2144 1486
Fragments arranged by size:
2144 1486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 4046
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 318 318
Size: 4046
BseYI C'CCAG_C
Cuts at: 404 1471 2229 2806 3302 404
Size: 1067 758 577 496 1148
Fragments arranged by size:
1148 1067 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 813
Size: 4046
BsiEI CG_ry'CG
Cuts at: 104 437 1588 1634 2842 104
Size: 333 1151 46 1208 1308
Fragments arranged by size:
1308 1208 1151 333 46
BsiHKAI G_wGCw'C
Cuts at: 1060 1838 2028 2820 1060
Size: 778 190 792 2286
Fragments arranged by size:
2286 792 778 190
BsmI GAATG_Cn'
Cuts at: 576 576
Size: 4046
Bsp1286I G_dGCh'C
Cuts at: 1008 1060 1671 1764 1838 2028 2090 2820 1008
Size: 52 611 93 74 190 62 730 2234
Fragments arranged by size:
2234 730 611 190 93 74 62 52
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 353 3222 353
Size: 2869 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 517 835 1096 1310 1612 1993 517
Size: 318 261 214 302 381 2570
Fragments arranged by size:
2570 381 318 302 261 214
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1958 1958
Size: 4046
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BsrGI T'GTAC_A
Cuts at: 284 284
Size: 4046
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstYI r'GATC_y
Cuts at: 1 718 1071 1538 1561 1896 2142 3154 3240
Size: 717 353 467 23 335 246 1012 86
Cuts at: 3240 3252 3598 1
Size: 12 346 449
Fragments arranged by size:
1012 717 467 449 353 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 364 982 364
Size: 618 3428
BtgI C'CryG_G
Cuts at: 107 458 944 107
Size: 351 486 3209
Fragments arranged by size:
3209 486 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 842 1289 842
Size: 447 3599
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DraIII CAC_nnn'GTG
Cuts at: 740 740
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 434 479 731 1631 1805 2196 2223 3568 434
Size: 45 252 900 174 391 27 1345 912
Fragments arranged by size:
1345 912 900 391 252 174 45 27
EagI C'GGCC_G
Cuts at: 434 1631 434
Size: 1197 2849
EarI CTCTTCn'nnn_
Cuts at: 717 1006 2069 2279 717
Size: 289 1063 210 2484
Fragments arranged by size:
2484 1063 289 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 805 1030 1871 2303 3050 3318 542
Size: 263 225 841 432 747 268 1270
Fragments arranged by size:
1270 841 747 432 268 263 225
EcoO109I rG'GnC_Cy
Cuts at: 1185 1185
Size: 4046
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 4046
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 1480 1728 2750 3729 1480
Size: 248 1022 979 1797
Fragments arranged by size:
1797 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 515 547 1888 1918 1920 1950 515
Size: 32 1341 30 2 30 2611
Fragments arranged by size:
2611 1341 32 30 30 2
HincII GTy'rAC
Cuts at: 1085 1085
Size: 4046
HindIII A'AGCT_T
Cuts at: 664 664
Size: 4046
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2717 2901 2717
Size: 184 3862
MscI TGG'CCA
Cuts at: 481 733 1807 481
Size: 252 1074 2720
Fragments arranged by size:
2720 1074 252
MslI CAynn'nnrTG
Cuts at: 130 223 352 538 883 946 2035 2162 130
Size: 93 129 186 345 63 1089 127 2014
Fragments arranged by size:
2014 1089 345 186 129 127 93 63
MspA1I CmG'CkG
Cuts at: 1157 1471 1831 2844 3089 1157
Size: 314 360 1013 245 2114
Fragments arranged by size:
2114 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NcoI C'CATG_G
Cuts at: 107 458 107
Size: 351 3695
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NspI r_CATG'y
Cuts at: 516 549 1099 1599 2506 516
Size: 33 550 500 907 2056
Fragments arranged by size:
2056 907 550 500 33
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PstI C_TGCA'G
Cuts at: 849 1093 1778 849
Size: 244 685 3117
Fragments arranged by size:
3117 685 244
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 1471 1831 1471
Size: 360 3686
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 170 416 845 975 1089 1774 2767 2958
Size: 137 246 429 130 114 685 993 191
Cuts at: 2958 33
Size: 1121
Fragments arranged by size:
1121 993 685 429 246 191 137 130
114
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 1201 2116 2608 2870 1201
Size: 915 492 262 2377
Fragments arranged by size:
2377 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
SspI AAT'ATT
Cuts at: 1034 1034
Size: 4046
StuI AGG'CCT
Cuts at: 817 817
Size: 4046
StyI C'CwwG_G
Cuts at: 107 458 800 1180 1534 107
Size: 351 342 380 354 2619
Fragments arranged by size:
2619 380 354 351 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 547 1629 1762 2221 2430 294
Size: 253 1082 133 459 209 1910
Fragments arranged by size:
1910 1082 459 253 209 133
TatI w'GTAC_w
Cuts at: 284 3561 284
Size: 3277 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4014
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1541 1541
Size: 4046
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
XmnI GAAnn'nnTTC
Cuts at: 698 698
Size: 4046
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI