Restriction map for
pF1KB7259
(Circular) (Six-base) MAPSORT of: pF1KB7259.seq Check: 7023 from: 1 to: 4871
pF1KB7259 4871 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 414 414
Size: 4871
AccI GT'mk_AC
Cuts at: 702 1781 1909 702
Size: 1079 128 3664
Fragments arranged by size:
3664 1079 128
Acc65I G'GTAC_C
Cuts at: 1887 1887
Size: 4871
AclI AA'CG_TT
Cuts at: 4711 4711
Size: 4871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1247 2696 3128 3875 4143 1247
Size: 1449 432 747 268 1975
Fragments arranged by size:
1975 1449 747 432 268
AfeI AGC'GCT
Cuts at: 175 193 175
Size: 18 4853
AflII C'TTAA_G
Cuts at: 945 1194 945
Size: 249 4622
AflIII A'CryG_T
Cuts at: 2223 3327 4304 2223
Size: 1104 977 2790
Fragments arranged by size:
2790 1104 977
AgeI A'CCGG_T
Cuts at: 3259 3259
Size: 4871
AhdI GACnn_n'nnGTC
Cuts at: 915 1014 915
Size: 99 4772
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 247 279 247
Size: 32 4839
AlwNI CAG_nnn'CTG
Cuts at: 2176 3743 2176
Size: 1567 3304
ApaI G_GGCC'C
Cuts at: 325 325
Size: 4871
ApaLI G'TGCA_C
Cuts at: 129 433 3641 129
Size: 304 3208 1359
Fragments arranged by size:
3208 1359 304
ApoI r'AATT_y
Cuts at: 343 1224 1690 1875 4224 4480 343
Size: 881 466 185 2349 256 734
Fragments arranged by size:
2349 881 734 466 256 185
AseI AT'TA_AT
Cuts at: 20 1571 20
Size: 1551 3320
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
AvaI C'yCGr_G
Cuts at: 157 855 1891 157
Size: 698 1036 3137
Fragments arranged by size:
3137 1036 698
BamHI G'GATC_C
Cuts at: 1896 1896
Size: 4871
BanI G'GyrC_C
Cuts at: 846 1067 1309 1669 1887 2301 2549 2584 846
Size: 221 242 360 218 414 248 35 3133
Fragments arranged by size:
3133 414 360 248 242 221 218 35
BanII G_rGCy'C
Cuts at: 325 840 1300 1885 2915 325
Size: 515 460 585 1030 2281
Fragments arranged by size:
2281 1030 585 515 460
BbeI G_GCGC'C
Cuts at: 1071 2305 2553 1071
Size: 1234 248 3389
Fragments arranged by size:
3389 1234 248
BbsI GAAGACnn'nnnn_
Cuts at: 690 911 1283 1616 1813 690
Size: 221 372 333 197 3748
Fragments arranged by size:
3748 372 333 221 197
BbvCI CC'TCA_GC
Cuts at: 292 292
Size: 4871
BciVI GTATCCnnnnn_n'
Cuts at: 2769 3530 4871 2769
Size: 761 1341 2769
Fragments arranged by size:
2769 1341 761
BclI T'GATC_A
Cuts at: 2391 2391
Size: 4871
BfrBI ATG'CAT
Cuts at: 1501 1501
Size: 4871
BglII A'GATC_T
Cuts at: 1428 2386 4423 1428
Size: 958 2037 1876
Fragments arranged by size:
2037 1876 958
BlpI GC'TnA_GC
Cuts at: 1983 1983
Size: 4871
Bme1580I G_kGCm'C
Cuts at: 133 325 437 1292 1674 2496 2589 3645 133
Size: 192 112 855 382 822 93 1056 1359
Fragments arranged by size:
1359 1056 855 822 382 192 112 93
BmrI ACTGGGnnnn_n'
Cuts at: 1411 2083 2164 2499 4643 1411
Size: 672 81 335 2144 1639
Fragments arranged by size:
2144 1639 672 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 516 3238 516
Size: 2722 2149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 490 783 900 1538 1804 249
Size: 241 293 117 638 266 3316
Fragments arranged by size:
3316 638 293 266 241 117
Bpu10I CC'TnA_GC
Cuts at: 292 839 1043 1388 1660 292
Size: 547 204 345 272 3503
Fragments arranged by size:
3503 547 345 272 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1359 1417 2047 2926 3418 3716 1359
Size: 58 630 879 492 298 2514
Fragments arranged by size:
2514 879 630 492 298 58
BsaI GGTCTCn'nnnn_
Cuts at: 4575 4575
Size: 4871
BsaAI yAC'GTr
Cuts at: 1679 2224 1679
Size: 545 4326
BsaBI GATnn'nnATC
Cuts at: 6 971 2385 4422 6
Size: 965 1414 2037 455
Fragments arranged by size:
2037 1414 965 455
BsaHI Gr'CG_yC
Cuts at: 1068 2302 2550 3252 4748 1068
Size: 1234 248 702 1496 1191
Fragments arranged by size:
1496 1234 1191 702 248
BsaWI w'CCGG_w
Cuts at: 2060 2581 3259 3533 3680 4091 2060
Size: 521 678 274 147 411 2840
Fragments arranged by size:
2840 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 247 277 496 526 1014 1044 1802 1832 247
Size: 30 219 30 488 30 758 30 3286
Fragments arranged by size:
3286 758 488 219 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 504 558 1090 1451 1651 504
Size: 54 532 361 200 3724
Fragments arranged by size:
3724 532 361 200 54
BseYI C'CCAG_C
Cuts at: 325 2296 3054 3631 4127 325
Size: 1971 758 577 496 1069
Fragments arranged by size:
1971 1069 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 500 673 323
Size: 177 173 4521
Fragments arranged by size:
4521 177 173
BsiEI CG_ry'CG
Cuts at: 104 2413 2459 3667 104
Size: 2309 46 1208 1308
Fragments arranged by size:
2309 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 133 437 840 1643 1828 1885 2663 2853 3645
Size: 304 403 803 185 57 778 190 792
Cuts at: 3645 133
Size: 1359
Fragments arranged by size:
1359 803 792 778 403 304 190 185
57
BsmI GAATG_Cn'
Cuts at: 1117 1117
Size: 4871
Bsp1286I G_dGCh'C
Cuts at: 133 325 437 840 1292 1300 1643 1674 1828
Size: 192 112 403 452 8 343 31 154
Cuts at: 1828 1885 2496 2589 2663 2853 2915 3645 133
Size: 57 611 93 74 190 62 730 1359
Fragments arranged by size:
1359 730 611 452 403 343 192 190
154 112 93 74 62 57 31 8
BspEI T'CCGG_A
Cuts at: 4091 4091
Size: 4871
BspHI T'CATG_A
Cuts at: 4047 4047
Size: 4871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 414 1226 1921 2135 2437 2818 414
Size: 812 695 214 302 381 2467
Fragments arranged by size:
2467 812 695 381 302 214
BsrBI CCG'CTC
Cuts at: 50 224 792 3163 3217 4701 4866 50
Size: 174 568 2371 54 1484 165 55
Fragments arranged by size:
2371 1484 568 174 165 55 54
BsrDI GCAATG_nn'
Cuts at: 928 2783 928
Size: 1855 3016
BsrFI r'CCGG_y
Cuts at: 133 2869 3050 3259 133
Size: 2736 181 209 1745
Fragments arranged by size:
2736 1745 209 181
BssSI C'ACGA_G
Cuts at: 3142 3500 3142
Size: 358 4513
BstAPI GCAn_nnn'nTGC
Cuts at: 420 2248 420
Size: 1828 3043
BstBI TT'CG_AA
Cuts at: 3232 3232
Size: 4871
BstXI CCAn_nnnn'nTGG
Cuts at: 807 832 807
Size: 25 4846
BstYI r'GATC_y
Cuts at: 1 1428 1896 2363 2386 2721 2967 3979 4065
Size: 1427 468 467 23 335 246 1012 86
Cuts at: 4065 4077 4423 1
Size: 12 346 449
Fragments arranged by size:
1427 1012 468 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 863 863
Size: 4871
BtgI C'CryG_G
Cuts at: 488 825 488
Size: 337 4534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2821 3002 3165 2821
Size: 181 163 4527
Fragments arranged by size:
4527 181 163
BtsI GCAGTG_nn'
Cuts at: 131 311 837 1329 1671 2114 131
Size: 180 526 492 342 443 2888
Fragments arranged by size:
2888 526 492 443 342 180
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1060 1095 1060
Size: 35 4836
DraI TTT'AAA
Cuts at: 1870 1870
Size: 4871
DraIII CAC_nnn'GTG
Cuts at: 1093 1093
Size: 4871
DrdI GACnn_nn'nnGTC
Cuts at: 2577 3435 2577
Size: 858 4013
EaeI y'GGCC_r
Cuts at: 401 462 1453 2456 2630 3021 3048 4393 401
Size: 61 991 1003 174 391 27 1345 879
Fragments arranged by size:
1345 1003 991 879 391 174 61 27
EagI C'GGCC_G
Cuts at: 2456 2456
Size: 4871
EarI CTCTTCn'nnn_
Cuts at: 646 2894 3104 646
Size: 2248 210 2413
Fragments arranged by size:
2413 2248 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3079 3389 3535 4681 3079
Size: 310 146 1146 3269
Fragments arranged by size:
3269 1146 310 146
EcoICRI GAG'CTC
Cuts at: 838 1883 838
Size: 1045 3826
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 490 783 900 1247 1538 1804 2696 3128
Size: 241 293 117 347 291 266 892 432
Cuts at: 3128 3875 4143 249
Size: 747 268 977
Fragments arranged by size:
977 892 747 432 347 293 291 268
266 241 117
EcoO109I rG'GnC_Cy
Cuts at: 322 1514 2010 322
Size: 1192 496 3183
Fragments arranged by size:
3183 1192 496
EcoRI G'AATT_C
Cuts at: 1875 1875
Size: 4871
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 4871
FspI TGC'GCA
Cuts at: 2652 2652
Size: 4871
HaeII r_GCGC'y
Cuts at: 177 195 1071 2305 2553 3575 4554 177
Size: 18 876 1234 248 1022 979 494
Fragments arranged by size:
1234 1022 979 876 494 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 709 741 835 867 901 933 1000 1032 1800
Size: 32 94 32 34 32 67 32 768
Cuts at: 1800 1832 2713 2743 2745 2775 709
Size: 32 881 30 2 30 2805
Fragments arranged by size:
2805 881 768 94 67 34 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 777 1910 777
Size: 1133 3738
KasI G'GCGC_C
Cuts at: 1067 2301 2549 1067
Size: 1234 248 3389
Fragments arranged by size:
3389 1234 248
KpnI G_GTAC'C
Cuts at: 1891 1891
Size: 4871
MluI A'CGCG_T
Cuts at: 4304 4304
Size: 4871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 692 1043 3542 3726 692
Size: 351 2499 184 1837
Fragments arranged by size:
2499 1837 351 184
MscI TGG'CCA
Cuts at: 403 464 2632 403
Size: 61 2168 2642
Fragments arranged by size:
2642 2168 61
MslI CAynn'nnrTG
Cuts at: 280 830 1354 1630 2860 2987 280
Size: 550 524 276 1230 127 2164
Fragments arranged by size:
2164 1230 550 524 276 127
MspA1I CmG'CkG
Cuts at: 1982 2296 2656 3669 3914 1982
Size: 314 360 1013 245 2939
Fragments arranged by size:
2939 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3052 3052
Size: 4871
NarI GG'CG_CC
Cuts at: 1068 2302 2550 1068
Size: 1234 248 3389
Fragments arranged by size:
3389 1234 248
NcoI C'CATG_G
Cuts at: 488 825 488
Size: 337 4534
NgoMIV G'CCGG_C
Cuts at: 3050 3050
Size: 4871
NsiI A_TGCA'T
Cuts at: 1503 1503
Size: 4871
NspI r_CATG'y
Cuts at: 319 1501 1924 2424 3331 319
Size: 1182 423 500 907 1859
Fragments arranged by size:
1859 1182 907 500 423
PasI CC'CwG_GG
Cuts at: 905 905
Size: 4871
PciI A'CATG_T
Cuts at: 3327 3327
Size: 4871
PfoI T'CCnGG_A
Cuts at: 1490 1490
Size: 4871
PmeI GTTT'AAAC
Cuts at: 1870 1870
Size: 4871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 247 279 4010 4042 247
Size: 32 3731 32 1076
Fragments arranged by size:
3731 1076 32 32
PpuMI rG'GwC_Cy
Cuts at: 1514 1514
Size: 4871
PspOMI G'GGCC_C
Cuts at: 321 321
Size: 4871
PspXI vC'TCGA_Gb
Cuts at: 855 855
Size: 4871
PstI C_TGCA'G
Cuts at: 524 788 1336 1918 2603 524
Size: 264 548 582 685 2792
Fragments arranged by size:
2792 685 582 548 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4871
PvuII CAG'CTG
Cuts at: 2296 2656 2296
Size: 360 4511
RsrII CG'GwC_CG
Cuts at: 3066 3066
Size: 4871
SacI G_AGCT'C
Cuts at: 840 1885 840
Size: 1045 3826
SalI G'TCGA_C
Cuts at: 1908 1908
Size: 4871
SapI GCTCTTCn'nnn_
Cuts at: 2894 3104 2894
Size: 210 4661
SbfI CC_TGCA'GG
Cuts at: 1918 1918
Size: 4871
ScaI AGT'ACT
Cuts at: 247 4388 247
Size: 4141 730
SexAI A'CCwGG_T
Cuts at: 395 395
Size: 4871
SfcI C'TryA_G
Cuts at: 33 169 249 520 784 1332 1914 2599 3592
Size: 136 80 271 264 548 582 685 993
Cuts at: 3592 3783 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 582 548 271 264 191
136 80
SfoI GGC'GCC
Cuts at: 1069 2303 2551 1069
Size: 1234 248 3389
Fragments arranged by size:
3389 1234 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
SmaI CCC'GGG
Cuts at: 1893 1893
Size: 4871
SmlI C'TyrA_G
Cuts at: 855 945 1194 1374 1432 2026 2941 3433 3695
Size: 90 249 180 58 594 915 492 262
Cuts at: 3695 855
Size: 2031
Fragments arranged by size:
2031 915 594 492 262 249 180 90
58
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4871
SphI G_CATG'C
Cuts at: 1924 2424 1924
Size: 500 4371
StuI AGG'CCT
Cuts at: 861 861
Size: 4871
StyI C'CwwG_G
Cuts at: 459 488 825 2005 2359 459
Size: 29 337 1180 354 2971
Fragments arranged by size:
2971 1180 354 337 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 420 918 2454 2587 3046 3255 420
Size: 498 1536 133 459 209 2036
Fragments arranged by size:
2036 1536 498 459 209 133
TatI w'GTAC_w
Cuts at: 245 1084 4386 245
Size: 839 3302 730
Fragments arranged by size:
3302 839 730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1014 1046 1107 1139 1802 1834 4388 4420 1014
Size: 32 61 32 663 32 2554 32 1465
Fragments arranged by size:
2554 1465 663 61 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1786 2668 1786
Size: 882 3989
XbaI T'CTAG_A
Cuts at: 1902 1902
Size: 4871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 472 2366 472
Size: 1894 2977
XhoI C'TCGA_G
Cuts at: 855 855
Size: 4871
XmaI C'CCGG_G
Cuts at: 1891 1891
Size: 4871
XmnI GAAnn'nnTTC
Cuts at: 355 355
Size: 4871
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AleI AlfI AscI AvrII BaeI BcgI BglI
BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PflMI PmlI PshAI
PsiI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI