Restriction map for
pF1KB7261
(Circular) (Six-base) MAPSORT of: pF1KB7261.seq Check: 6531 from: 1 to: 3701
pF1KB7261 3701 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 437 739 437
Size: 302 3399
Acc65I G'GTAC_C
Cuts at: 717 717
Size: 3701
AclI AA'CG_TT
Cuts at: 3541 3541
Size: 3701
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1526 1958 2705 2973 1526
Size: 432 747 268 2254
Fragments arranged by size:
2254 747 432 268
AflIII A'CryG_T
Cuts at: 1053 2157 3134 1053
Size: 1104 977 1620
Fragments arranged by size:
1620 1104 977
AgeI A'CCGG_T
Cuts at: 2089 2089
Size: 3701
AhdI GACnn_n'nnGTC
Cuts at: 444 444
Size: 3701
AleI CACnn'nnGTG
Cuts at: 688 688
Size: 3701
AlwNI CAG_nnn'CTG
Cuts at: 1006 2573 1006
Size: 1567 2134
ApaLI G'TGCA_C
Cuts at: 2471 2471
Size: 3701
ApoI r'AATT_y
Cuts at: 705 3054 3310 705
Size: 2349 256 1096
Fragments arranged by size:
2349 1096 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3701
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
AvaI C'yCGr_G
Cuts at: 331 721 331
Size: 390 3311
BamHI G'GATC_C
Cuts at: 726 726
Size: 3701
BanI G'GyrC_C
Cuts at: 717 1131 1379 1414 717
Size: 414 248 35 3004
Fragments arranged by size:
3004 414 248 35
BanII G_rGCy'C
Cuts at: 303 715 1745 303
Size: 412 1030 2259
Fragments arranged by size:
2259 1030 412
BbeI G_GCGC'C
Cuts at: 1135 1383 1135
Size: 248 3453
BciVI GTATCCnnnnn_n'
Cuts at: 139 1599 2360 3701 139
Size: 1460 761 1341 139
Fragments arranged by size:
1460 1341 761 139
BclI T'GATC_A
Cuts at: 1221 1221
Size: 3701
BglII A'GATC_T
Cuts at: 1216 3253 1216
Size: 2037 1664
BlpI GC'TnA_GC
Cuts at: 813 813
Size: 3701
Bme1580I G_kGCm'C
Cuts at: 332 1326 1419 2475 332
Size: 994 93 1056 1558
Fragments arranged by size:
1558 1056 994 93
BmgBI CAC'GTC
Cuts at: 446 446
Size: 3701
BmrI ACTGGGnnnn_n'
Cuts at: 913 994 1329 3473 913
Size: 81 335 2144 1141
Fragments arranged by size:
2144 1141 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 276 2068 276
Size: 1792 1909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 271 223
Size: 48 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1756 2248 2546 877
Size: 879 492 298 2032
Fragments arranged by size:
2032 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3405 3405
Size: 3701
BsaAI yAC'GTr
Cuts at: 1054 1054
Size: 3701
BsaBI GATnn'nnATC
Cuts at: 6 1215 3252 6
Size: 1209 2037 455
Fragments arranged by size:
2037 1209 455
BsaHI Gr'CG_yC
Cuts at: 1132 1380 2082 3578 1132
Size: 248 702 1496 1255
Fragments arranged by size:
1496 1255 702 248
BsaWI w'CCGG_w
Cuts at: 663 890 1411 2089 2363 2510 2921 663
Size: 227 521 678 274 147 411 1443
Fragments arranged by size:
1443 678 521 411 274 227 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 124 637 124
Size: 513 3188
BseYI C'CCAG_C
Cuts at: 1126 1884 2461 2957 1126
Size: 758 577 496 1870
Fragments arranged by size:
1870 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 477
Size: 3701
BsiEI CG_ry'CG
Cuts at: 104 1243 1289 2497 104
Size: 1139 46 1208 1308
Fragments arranged by size:
1308 1208 1139 46
BsiHKAI G_wGCw'C
Cuts at: 303 715 1493 1683 2475 303
Size: 412 778 190 792 1529
Fragments arranged by size:
1529 792 778 412 190
Bsp1286I G_dGCh'C
Cuts at: 303 332 715 1326 1419 1493 1683 1745 2475
Size: 29 383 611 93 74 190 62 730
Cuts at: 2475 303
Size: 1529
Fragments arranged by size:
1529 730 611 383 190 93 74 62
29
BspEI T'CCGG_A
Cuts at: 663 2921 663
Size: 2258 1443
BspHI T'CATG_A
Cuts at: 2877 2877
Size: 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 751 965 1267 1648 751
Size: 214 302 381 2804
Fragments arranged by size:
2804 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1993 2047 3531 3696 50
Size: 1943 54 1484 165 55
Fragments arranged by size:
1943 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1613 1613
Size: 3701
BsrFI r'CCGG_y
Cuts at: 496 1699 1880 2089 496
Size: 1203 181 209 2108
Fragments arranged by size:
2108 1203 209 181
BssSI C'ACGA_G
Cuts at: 510 1972 2330 510
Size: 1462 358 1881
Fragments arranged by size:
1881 1462 358
BstAPI GCAn_nnn'nTGC
Cuts at: 500 1078 500
Size: 578 3123
BstBI TT'CG_AA
Cuts at: 2062 2062
Size: 3701
BstYI r'GATC_y
Cuts at: 1 179 218 726 1193 1216 1551 1797 2809
Size: 178 39 508 467 23 335 246 1012
Cuts at: 2809 2895 2907 3253 1
Size: 86 12 346 449
Fragments arranged by size:
1012 508 467 449 346 335 246 178
86 39 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1651 1832 1995 1651
Size: 181 163 3357
Fragments arranged by size:
3357 181 163
BtsI GCAGTG_nn'
Cuts at: 522 944 522
Size: 422 3279
DraI TTT'AAA
Cuts at: 700 700
Size: 3701
DrdI GACnn_nn'nnGTC
Cuts at: 1407 2265 1407
Size: 858 2843
EaeI y'GGCC_r
Cuts at: 164 563 1286 1460 1851 1878 3223 164
Size: 399 723 174 391 27 1345 642
Fragments arranged by size:
1345 723 642 399 391 174 27
EagI C'GGCC_G
Cuts at: 1286 1286
Size: 3701
EarI CTCTTCn'nnn_
Cuts at: 355 671 1724 1934 355
Size: 316 1053 210 2122
Fragments arranged by size:
2122 1053 316 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 438 1909 2219 2365 3511 438
Size: 1471 310 146 1146 628
Fragments arranged by size:
1471 1146 628 310 146
EcoICRI GAG'CTC
Cuts at: 301 713 301
Size: 412 3289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 271 1526 1958 2705 2973 223
Size: 48 1255 432 747 268 951
Fragments arranged by size:
1255 951 747 432 268 48
EcoO109I rG'GnC_Cy
Cuts at: 113 234 840 113
Size: 121 606 2974
Fragments arranged by size:
2974 606 121
EcoRI G'AATT_C
Cuts at: 705 705
Size: 3701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1720 1720
Size: 3701
FspI TGC'GCA
Cuts at: 1482 1482
Size: 3701
HaeII r_GCGC'y
Cuts at: 1135 1383 2405 3384 1135
Size: 248 1022 979 1452
Fragments arranged by size:
1452 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 1543 1573 1575 1605 430
Size: 32 1081 30 2 30 2526
Fragments arranged by size:
2526 1081 32 30 30 2
HincII GTy'rAC
Cuts at: 438 740 438
Size: 302 3399
KasI G'GCGC_C
Cuts at: 1131 1379 1131
Size: 248 3453
KpnI G_GTAC'C
Cuts at: 721 721
Size: 3701
MluI A'CGCG_T
Cuts at: 3134 3134
Size: 3701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2372 2556 2372
Size: 184 3517
MscI TGG'CCA
Cuts at: 565 1462 565
Size: 897 2804
MslI CAynn'nnrTG
Cuts at: 688 1690 1817 688
Size: 1002 127 2572
Fragments arranged by size:
2572 1002 127
MspA1I CmG'CkG
Cuts at: 388 812 1126 1486 2499 2744 388
Size: 424 314 360 1013 245 1345
Fragments arranged by size:
1345 1013 424 360 314 245
NaeI GCC'GGC
Cuts at: 1882 1882
Size: 3701
NarI GG'CG_CC
Cuts at: 1132 1380 1132
Size: 248 3453
NgoMIV G'CCGG_C
Cuts at: 1880 1880
Size: 3701
NspI r_CATG'y
Cuts at: 328 754 1254 2161 328
Size: 426 500 907 1868
Fragments arranged by size:
1868 907 500 426
PciI A'CATG_T
Cuts at: 2157 2157
Size: 3701
PflMI CCAn_nnn'nTGG
Cuts at: 162 162
Size: 3701
PfoI T'CCnGG_A
Cuts at: 230 248 230
Size: 18 3683
PmeI GTTT'AAAC
Cuts at: 700 700
Size: 3701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2840 2872 2840
Size: 32 3669
PpuMI rG'GwC_Cy
Cuts at: 113 234 113
Size: 121 3580
PstI C_TGCA'G
Cuts at: 748 1433 748
Size: 685 3016
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3701
PvuII CAG'CTG
Cuts at: 1126 1486 1126
Size: 360 3341
RsrII CG'GwC_CG
Cuts at: 1896 1896
Size: 3701
SacI G_AGCT'C
Cuts at: 303 715 303
Size: 412 3289
SalI G'TCGA_C
Cuts at: 436 738 436
Size: 302 3399
SapI GCTCTTCn'nnn_
Cuts at: 1724 1934 1724
Size: 210 3491
SbfI CC_TGCA'GG
Cuts at: 748 748
Size: 3701
ScaI AGT'ACT
Cuts at: 3218 3218
Size: 3701
SfcI C'TryA_G
Cuts at: 33 744 1429 2422 2613 33
Size: 711 685 993 191 1121
Fragments arranged by size:
1121 993 711 685 191
SfoI GGC'GCC
Cuts at: 1133 1381 1133
Size: 248 3453
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
SmaI CCC'GGG
Cuts at: 723 723
Size: 3701
SmlI C'TyrA_G
Cuts at: 856 1771 2263 2525 856
Size: 915 492 262 2032
Fragments arranged by size:
2032 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3701
SphI G_CATG'C
Cuts at: 754 1254 754
Size: 500 3201
StyI C'CwwG_G
Cuts at: 116 566 835 1189 116
Size: 450 269 354 2628
Fragments arranged by size:
2628 450 354 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1284 1417 1876 2085 1284
Size: 133 459 209 2900
Fragments arranged by size:
2900 459 209 133
TatI w'GTAC_w
Cuts at: 3216 3216
Size: 3701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 3669
Tth111I GACn'n_nGTC
Cuts at: 1498 1498
Size: 3701
XbaI T'CTAG_A
Cuts at: 732 732
Size: 3701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1196 114
Size: 1082 2619
XmaI C'CCGG_G
Cuts at: 721 721
Size: 3701
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI