Restriction map for
pF1KB7265
(Circular) (Six-base) MAPSORT of: pF1KB7265.seq Check: 3730 from: 1 to: 4169
pF1KB7265 4169 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 780 1128 780
Size: 348 3821
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 778 1185 778
Size: 407 3762
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 772 1076 1994 2426 3173 3441 481
Size: 291 304 918 432 747 268 1209
Fragments arranged by size:
1209 918 747 432 304 291 268
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 818 818
Size: 4169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 115 147 115
Size: 32 4137
AlwNI CAG_nnn'CTG
Cuts at: 675 1474 3041 675
Size: 799 1567 1803
Fragments arranged by size:
1803 1567 799
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 1173 3522 3778 1173
Size: 2349 256 1564
Fragments arranged by size:
2349 1564 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 1189 1189
Size: 4169
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 559 778 822 1185 1599 1847 1882 559
Size: 219 44 363 414 248 35 2846
Fragments arranged by size:
2846 414 363 248 219 44 35
BanII G_rGCy'C
Cuts at: 998 1183 2213 998
Size: 185 1030 2954
Fragments arranged by size:
2954 1030 185
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BbsI GAAGACnn'nnnn_
Cuts at: 668 668
Size: 4169
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BglII A'GATC_T
Cuts at: 632 1684 3721 632
Size: 1052 2037 1080
Fragments arranged by size:
2037 1080 1052
BlpI GC'TnA_GC
Cuts at: 142 613 992 1281 142
Size: 471 379 289 3030
Fragments arranged by size:
3030 471 379 289
Bme1580I G_kGCm'C
Cuts at: 1794 1887 2943 1794
Size: 93 1056 3020
Fragments arranged by size:
3020 1056 93
BmgBI CAC'GTC
Cuts at: 948 948
Size: 4169
BmrI ACTGGGnnnn_n'
Cuts at: 1053 1381 1462 1797 3941 1053
Size: 328 81 335 2144 1281
Fragments arranged by size:
2144 1281 335 328 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1039 532
Size: 507 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 2224 2716 3014 1345
Size: 879 492 298 2500
Fragments arranged by size:
2500 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3873 3873
Size: 4169
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 649 1600 1848 2550 4046 649
Size: 951 248 702 1496 772
Fragments arranged by size:
1496 951 772 702 248
BsaWI w'CCGG_w
Cuts at: 378 1031 1358 1879 2557 2831 2978 3389 378
Size: 653 327 521 678 274 147 411 1158
Fragments arranged by size:
1158 678 653 521 411 327 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 115 145 910 940 115
Size: 30 765 30 3344
Fragments arranged by size:
3344 765 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 754 923 754
Size: 169 4000
BseYI C'CCAG_C
Cuts at: 714 957 1084 1594 2352 2929 3425 714
Size: 243 127 510 758 577 496 1458
Fragments arranged by size:
1458 758 577 510 496 243 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 814
Size: 4169
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 998 1043 1183 1961 2151 2943 998
Size: 45 140 778 190 792 2224
Fragments arranged by size:
2224 792 778 190 140 45
BsmI GAATG_Cn'
Cuts at: 970 970
Size: 4169
Bsp1286I G_dGCh'C
Cuts at: 998 1043 1183 1794 1887 1961 2151 2213 2943
Size: 45 140 611 93 74 190 62 730
Cuts at: 2943 998
Size: 2224
Fragments arranged by size:
2224 730 611 190 140 93 74 62
45
BspEI T'CCGG_A
Cuts at: 378 1031 3389 378
Size: 653 2358 1158
Fragments arranged by size:
2358 1158 653
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 780 1128 1219 1433 1735 2116 780
Size: 348 91 214 302 381 2833
Fragments arranged by size:
2833 381 348 302 214 91
BsrBI CCG'CTC
Cuts at: 50 2461 2515 3999 4164 50
Size: 2411 54 1484 165 55
Fragments arranged by size:
2411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 298 596 2167 2348 2557 298
Size: 298 1571 181 209 1910
Fragments arranged by size:
1910 1571 298 209 181
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1546
Size: 4169
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstYI r'GATC_y
Cuts at: 1 632 1194 1661 1684 2019 2265 3277 3363
Size: 631 562 467 23 335 246 1012 86
Cuts at: 3363 3375 3721 1
Size: 12 346 449
Fragments arranged by size:
1012 631 562 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 576 1412 576
Size: 836 3333
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 867 1754 1928 2319 2346 3691 867
Size: 887 174 391 27 1345 1345
Fragments arranged by size:
1345 1345 887 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 996 1181 996
Size: 185 3984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 532 772 1039 1076 1994 2426 3173 3441
Size: 51 240 267 37 918 432 747 268
Cuts at: 3441 481
Size: 1209
Fragments arranged by size:
1209 918 747 432 268 267 240 51
37
EcoNI CCTnn'n_nnAGG
Cuts at: 400 460 400
Size: 60 4109
EcoO109I rG'GnC_Cy
Cuts at: 1023 1074 1308 1023
Size: 51 234 3884
Fragments arranged by size:
3884 234 51
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
EcoRV GAT'ATC
Cuts at: 690 690
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 590 1603 1851 2873 3852 590
Size: 1013 248 1022 979 907
Fragments arranged by size:
1022 1013 979 907 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 239 271 303 910 942 1045 1077 2011 2041
Size: 32 32 607 32 103 32 934 30
Cuts at: 2041 2043 2073 239
Size: 2 30 2335
Fragments arranged by size:
2335 934 607 103 32 32 32 32
30 30 2
HincII GTy'rAC
Cuts at: 1208 1208
Size: 4169
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 782 1189 782
Size: 407 3762
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 854 2840 3024 854
Size: 1986 184 1999
Fragments arranged by size:
1999 1986 184
MscI TGG'CCA
Cuts at: 869 1930 869
Size: 1061 3108
MslI CAynn'nnrTG
Cuts at: 2158 2285 2158
Size: 127 4042
MspA1I CmG'CkG
Cuts at: 593 1280 1594 1954 2967 3212 593
Size: 687 314 360 1013 245 1550
Fragments arranged by size:
1550 1013 687 360 314 245
NaeI GCC'GGC
Cuts at: 598 2350 598
Size: 1752 2417
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NcoI C'CATG_G
Cuts at: 107 107
Size: 4169
NgoMIV G'CCGG_C
Cuts at: 596 2348 596
Size: 1752 2417
NspI r_CATG'y
Cuts at: 210 1222 1722 2629 210
Size: 1012 500 907 1750
Fragments arranged by size:
1750 1012 907 500
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 115 147 3308 3340 115
Size: 32 3161 32 944
Fragments arranged by size:
3161 944 32 32
PpuMI rG'GwC_Cy
Cuts at: 1023 1074 1023
Size: 51 4118
PstI C_TGCA'G
Cuts at: 1216 1901 1216
Size: 685 3484
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 593 1594 1954 593
Size: 1001 360 2808
Fragments arranged by size:
2808 1001 360
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 998 1183 998
Size: 185 3984
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SanDI GG'GwC_CC
Cuts at: 1074 1074
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 939 1212 1897 2890 3081 33
Size: 906 273 685 993 191 1121
Fragments arranged by size:
1121 993 906 685 273 191
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 1324 2239 2731 2993 1324
Size: 915 492 262 2500
Fragments arranged by size:
2500 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StuI AGG'CCT
Cuts at: 937 937
Size: 4169
StyI C'CwwG_G
Cuts at: 107 185 401 999 1124 1303 1657 107
Size: 78 216 598 125 179 354 2619
Fragments arranged by size:
2619 598 354 216 179 125 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 949 1752 1885 2344 2553 949
Size: 803 133 459 209 2565
Fragments arranged by size:
2565 803 459 209 133
TatI w'GTAC_w
Cuts at: 3684 3684
Size: 4169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 1027 1966 1027
Size: 939 3230
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1664 1664
Size: 4169
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
XmnI GAAnn'nnTTC
Cuts at: 127 634 127
Size: 507 3662
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI