Restriction map for
pF1KB7268
(Circular) (Six-base) MAPSORT of: pF1KB7268.seq Check: 1758 from: 1 to: 4757
pF1KB7268 4757 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 282 282
Size: 4757
AccI GT'mk_AC
Cuts at: 1795 1795
Size: 4757
Acc65I G'GTAC_C
Cuts at: 1773 1773
Size: 4757
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3014 3761 4029 2582
Size: 432 747 268 3310
Fragments arranged by size:
3310 747 432 268
AflIII A'CryG_T
Cuts at: 1632 2109 3213 4190 1632
Size: 477 1104 977 2199
Fragments arranged by size:
2199 1104 977 477
AgeI A'CCGG_T
Cuts at: 1285 3145 1285
Size: 1860 2897
AhdI GACnn_n'nnGTC
Cuts at: 1580 1580
Size: 4757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 155 155
Size: 4757
AlwNI CAG_nnn'CTG
Cuts at: 2062 3629 2062
Size: 1567 3190
ApaLI G'TGCA_C
Cuts at: 3527 3527
Size: 4757
ApoI r'AATT_y
Cuts at: 188 689 773 921 1109 1277 1361 1445 1696
Size: 501 84 148 188 168 84 84 251
Cuts at: 1696 1761 4110 4366 188
Size: 65 2349 256 579
Fragments arranged by size:
2349 579 501 256 251 188 168 148
84 84 84 65
AseI AT'TA_AT
Cuts at: 20 761 20
Size: 741 4016
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 1777 1777
Size: 4757
BamHI G'GATC_C
Cuts at: 1782 1782
Size: 4757
BanI G'GyrC_C
Cuts at: 270 1773 2187 2435 2470 270
Size: 1503 414 248 35 2557
Fragments arranged by size:
2557 1503 414 248 35
BanII G_rGCy'C
Cuts at: 392 1339 1771 2801 392
Size: 947 432 1030 2348
Fragments arranged by size:
2348 1030 947 432
BbeI G_GCGC'C
Cuts at: 2191 2439 2191
Size: 248 4509
BbsI GAAGACnn'nnnn_
Cuts at: 234 1579 234
Size: 1345 3412
BciVI GTATCCnnnnn_n'
Cuts at: 2655 3416 4757 2655
Size: 761 1341 2655
Fragments arranged by size:
2655 1341 761
BclI T'GATC_A
Cuts at: 626 2277 626
Size: 1651 3106
BfrBI ATG'CAT
Cuts at: 667 1135 1617 667
Size: 468 482 3807
Fragments arranged by size:
3807 482 468
BglII A'GATC_T
Cuts at: 2272 4309 2272
Size: 2037 2720
BlpI GC'TnA_GC
Cuts at: 1869 1869
Size: 4757
Bme1580I G_kGCm'C
Cuts at: 2382 2475 3531 2382
Size: 93 1056 3608
Fragments arranged by size:
3608 1056 93
BmgBI CAC'GTC
Cuts at: 300 300
Size: 4757
BmrI ACTGGGnnnn_n'
Cuts at: 343 1969 2050 2385 4529 343
Size: 1626 81 335 2144 571
Fragments arranged by size:
2144 1626 571 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 4757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1391 437
Size: 954 3803
Bpu10I CC'TnA_GC
Cuts at: 117 117
Size: 4757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 1330 1599 1933 2812 3304 3602 559
Size: 771 269 334 879 492 298 1714
Fragments arranged by size:
1714 879 771 492 334 298 269
BsaI GGTCTCn'nnnn_
Cuts at: 304 333 1425 4461 304
Size: 29 1092 3036 600
Fragments arranged by size:
3036 1092 600 29
BsaAI yAC'GTr
Cuts at: 2110 2110
Size: 4757
BsaBI GATnn'nnATC
Cuts at: 6 628 2271 4308 6
Size: 622 1643 2037 455
Fragments arranged by size:
2037 1643 622 455
BsaHI Gr'CG_yC
Cuts at: 2188 2436 3138 4634 2188
Size: 248 702 1496 2311
Fragments arranged by size:
2311 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1285 1946 2467 3145 3419 3566 3977 1285
Size: 661 521 678 274 147 411 2065
Fragments arranged by size:
2065 678 661 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2182 2940 3517 4013 2182
Size: 758 577 496 2926
Fragments arranged by size:
2926 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2299 2345 3553 104
Size: 2195 46 1208 1308
Fragments arranged by size:
2195 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1339 1771 2549 2739 3531 1339
Size: 432 778 190 792 2565
Fragments arranged by size:
2565 792 778 432 190
BsmI GAATG_Cn'
Cuts at: 574 1619 574
Size: 1045 3712
BsmBI CGTCTCn'nnnn_
Cuts at: 290 290
Size: 4757
Bsp1286I G_dGCh'C
Cuts at: 392 1339 1771 2382 2475 2549 2739 2801 3531
Size: 947 432 611 93 74 190 62 730
Cuts at: 3531 392
Size: 1618
Fragments arranged by size:
1618 947 730 611 432 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3977 3977
Size: 4757
BspHI T'CATG_A
Cuts at: 1584 3933 1584
Size: 2349 2408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 282 1807 2021 2323 2704 282
Size: 1525 214 302 381 2335
Fragments arranged by size:
2335 1525 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3049 3103 4587 4752 50
Size: 2999 54 1484 165 55
Fragments arranged by size:
2999 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1500 2669 1500
Size: 1169 3588
BsrFI r'CCGG_y
Cuts at: 1285 2755 2936 3145 1285
Size: 1470 181 209 2897
Fragments arranged by size:
2897 1470 209 181
BsrGI T'GTAC_A
Cuts at: 290 1434 290
Size: 1144 3613
BssSI C'ACGA_G
Cuts at: 3028 3386 3028
Size: 358 4399
BstAPI GCAn_nnn'nTGC
Cuts at: 2134 2134
Size: 4757
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstXI CCAn_nnnn'nTGG
Cuts at: 1723 1723
Size: 4757
BstYI r'GATC_y
Cuts at: 1 1082 1782 2249 2272 2607 2853 3865 3951
Size: 1081 700 467 23 335 246 1012 86
Cuts at: 3951 3963 4309 1
Size: 12 346 449
Fragments arranged by size:
1081 1012 700 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4757
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2707 2888 3051 2707
Size: 181 163 4413
Fragments arranged by size:
4413 181 163
BtsI GCAGTG_nn'
Cuts at: 268 2000 268
Size: 1732 3025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1128 1163 1296 1331 1128
Size: 35 133 35 4554
Fragments arranged by size:
4554 133 35 35
DraI TTT'AAA
Cuts at: 825 1756 825
Size: 931 3826
DrdI GACnn_nn'nnGTC
Cuts at: 2463 3321 2463
Size: 858 3899
EaeI y'GGCC_r
Cuts at: 233 2342 2516 2907 2934 4279 233
Size: 2109 174 391 27 1345 711
Fragments arranged by size:
2109 1345 711 391 174 27
EagI C'GGCC_G
Cuts at: 2342 2342
Size: 4757
EarI CTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2965 3275 3421 4567 2965
Size: 310 146 1146 3155
Fragments arranged by size:
3155 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1337 1769 1337
Size: 432 4325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1391 2582 3014 3761 4029 437
Size: 954 1191 432 747 268 1165
Fragments arranged by size:
1191 1165 954 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 171 1406 1896 171
Size: 1235 490 3032
Fragments arranged by size:
3032 1235 490
EcoRI G'AATT_C
Cuts at: 1109 1277 1361 1445 1761 1109
Size: 168 84 84 316 4105
Fragments arranged by size:
4105 316 168 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4757
FspI TGC'GCA
Cuts at: 2538 2538
Size: 4757
HaeII r_GCGC'y
Cuts at: 2191 2439 3461 4440 2191
Size: 248 1022 979 2508
Fragments arranged by size:
2508 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 349 381 1002 1034 1074 1106 1170
Size: 32 157 32 621 32 40 32 64
Cuts at: 1170 1202 1506 1538 1566 1598 2599 2629 2631
Size: 32 304 32 28 32 1001 30 2
Cuts at: 2631 2661 160
Size: 30 2256
Fragments arranged by size:
2256 1001 621 304 157 64 40 32
32 32 32 32 32 32 30 30
28 2
HincII GTy'rAC
Cuts at: 1796 1796
Size: 4757
KasI G'GCGC_C
Cuts at: 2187 2435 2187
Size: 248 4509
KpnI G_GTAC'C
Cuts at: 1777 1777
Size: 4757
MfeI C'AATT_G
Cuts at: 1510 1510
Size: 4757
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 287 3428 3612 287
Size: 3141 184 1432
Fragments arranged by size:
3141 1432 184
MscI TGG'CCA
Cuts at: 235 2518 235
Size: 2283 2474
MslI CAynn'nnrTG
Cuts at: 482 501 882 950 1118 1286 1454 1470 1721
Size: 19 381 68 168 168 168 16 251
Cuts at: 1721 2746 2873 482
Size: 1025 127 2366
Fragments arranged by size:
2366 1025 381 251 168 168 168 127
68 19 16
MspA1I CmG'CkG
Cuts at: 1868 2182 2542 3555 3800 1868
Size: 314 360 1013 245 2825
Fragments arranged by size:
2825 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 4757
NarI GG'CG_CC
Cuts at: 2188 2436 2188
Size: 248 4509
NcoI C'CATG_G
Cuts at: 107 107
Size: 4757
NdeI CA'TA_TG
Cuts at: 879 1467 1718 879
Size: 588 251 3918
Fragments arranged by size:
3918 588 251
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 4757
NsiI A_TGCA'T
Cuts at: 669 1137 1619 669
Size: 468 482 3807
Fragments arranged by size:
3807 482 468
NspI r_CATG'y
Cuts at: 481 667 1139 1636 1810 2310 3217 481
Size: 186 472 497 174 500 907 2021
Fragments arranged by size:
2021 907 500 497 472 186 174
PciI A'CATG_T
Cuts at: 1632 3213 1632
Size: 1581 3176
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4725
PpuMI rG'GwC_Cy
Cuts at: 171 171
Size: 4757
PstI C_TGCA'G
Cuts at: 1804 2489 1804
Size: 685 4072
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 2182 2542 2182
Size: 360 4397
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 1339 1771 1339
Size: 432 4325
SalI G'TCGA_C
Cuts at: 1794 1794
Size: 4757
SapI GCTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
SbfI CC_TGCA'GG
Cuts at: 1804 1804
Size: 4757
ScaI AGT'ACT
Cuts at: 4274 4274
Size: 4757
SexAI A'CCwGG_T
Cuts at: 323 323
Size: 4757
SfcI C'TryA_G
Cuts at: 33 315 1800 2485 3478 3669 33
Size: 282 1485 685 993 191 1121
Fragments arranged by size:
1485 1121 993 685 282 191
SfoI GGC'GCC
Cuts at: 2189 2437 2189
Size: 248 4509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 1779 1779
Size: 4757
SmlI C'TyrA_G
Cuts at: 538 1345 1578 1912 2827 3319 3581 538
Size: 807 233 334 915 492 262 1714
Fragments arranged by size:
1714 915 807 492 334 262 233
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 667 1139 1810 2310 667
Size: 472 671 500 3114
Fragments arranged by size:
3114 671 500 472
SspI AAT'ATT
Cuts at: 858 858
Size: 4757
StuI AGG'CCT
Cuts at: 422 820 904 988 1072 1324 422
Size: 398 84 84 84 252 3855
Fragments arranged by size:
3855 398 252 84 84 84
StyI C'CwwG_G
Cuts at: 107 207 455 1891 2245 107
Size: 100 248 1436 354 2619
Fragments arranged by size:
2619 1436 354 248 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2340 2473 2932 3141 2340
Size: 133 459 209 3956
Fragments arranged by size:
3956 459 209 133
TatI w'GTAC_w
Cuts at: 290 1098 1434 4272 290
Size: 808 336 2838 775
Fragments arranged by size:
2838 808 775 336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4274 4306 4274
Size: 32 4725
Tth111I GACn'n_nGTC
Cuts at: 2554 2554
Size: 4757
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2252 2252
Size: 4757
XmaI C'CCGG_G
Cuts at: 1777 1777
Size: 4757
XmnI GAAnn'nnTTC
Cuts at: 464 464
Size: 4757
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmtI BsaXI BseRI BsgI
BsiWI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI