Restriction map for
pF1KB7269
(Circular) (Six-base) MAPSORT of: pF1KB7269.seq Check: 5655 from: 1 to: 4406
pF1KB7269 4406 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 443 4246 443
Size: 3803 603
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 416 499 1196 2231 2663 3410 3678 177
Size: 239 83 697 1035 432 747 268 905
Fragments arranged by size:
1035 905 747 697 432 268 239 83
AflIII A'CryG_T
Cuts at: 1758 2862 3839 1758
Size: 1104 977 2325
Fragments arranged by size:
2325 1104 977
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1070 1102 1070
Size: 32 4374
AlwNI CAG_nnn'CTG
Cuts at: 1711 3278 1711
Size: 1567 2839
ApaI G_GGCC'C
Cuts at: 301 701 301
Size: 400 4006
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 932 1272 1410 3759 4015 932
Size: 340 138 2349 256 1323
Fragments arranged by size:
2349 1323 340 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 1426 1426
Size: 4406
BamHI G'GATC_C
Cuts at: 764 1198 1431 764
Size: 434 233 3739
Fragments arranged by size:
3739 434 233
BanI G'GyrC_C
Cuts at: 1422 1836 2084 2119 1422
Size: 414 248 35 3709
Fragments arranged by size:
3709 414 248 35
BanII G_rGCy'C
Cuts at: 301 701 1420 2450 301
Size: 400 719 1030 2257
Fragments arranged by size:
2257 1030 719 400
BbeI G_GCGC'C
Cuts at: 1840 2088 1840
Size: 248 4158
BbsI GAAGACnn'nnnn_
Cuts at: 1326 1326
Size: 4406
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 1926 1926
Size: 4406
BglII A'GATC_T
Cuts at: 1921 3958 1921
Size: 2037 2369
BlpI GC'TnA_GC
Cuts at: 1518 1518
Size: 4406
Bme1580I G_kGCm'C
Cuts at: 301 701 2031 2124 3180 301
Size: 400 1330 93 1056 1527
Fragments arranged by size:
1527 1330 1056 400 93
BmrI ACTGGGnnnn_n'
Cuts at: 614 950 1034 1152 1363 1618 1699 2034 4178
Size: 336 84 118 211 255 81 335 2144
Cuts at: 4178 614
Size: 842
Fragments arranged by size:
2144 842 336 335 255 211 118 84
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 883 2773 451
Size: 432 1890 2084
Fragments arranged by size:
2084 1890 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 295 794 1154 1177 206
Size: 89 499 360 23 3435
Fragments arranged by size:
3435 499 360 89 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1582 2461 2953 3251 1582
Size: 879 492 298 2737
Fragments arranged by size:
2737 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 1759 1759
Size: 4406
BsaBI GATnn'nnATC
Cuts at: 6 1920 3957 6
Size: 1914 2037 455
Fragments arranged by size:
2037 1914 455
BsaHI Gr'CG_yC
Cuts at: 1837 2085 2787 4283 1837
Size: 248 702 1496 1960
Fragments arranged by size:
1960 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1595 2116 2794 3068 3215 3626 1595
Size: 521 678 274 147 411 2375
Fragments arranged by size:
2375 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 212 1155 1386 212
Size: 943 231 3232
Fragments arranged by size:
3232 943 231
BseYI C'CCAG_C
Cuts at: 1831 2589 3166 3662 1831
Size: 758 577 496 2575
Fragments arranged by size:
2575 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1165 1256 1420 2198 2388 3180 1165
Size: 91 164 778 190 792 2391
Fragments arranged by size:
2391 792 778 190 164 91
BsmI GAATG_Cn'
Cuts at: 422 422
Size: 4406
Bsp1286I G_dGCh'C
Cuts at: 301 701 1165 1256 1420 2031 2124 2198 2388
Size: 400 464 91 164 611 93 74 190
Cuts at: 2388 2450 3180 301
Size: 62 730 1527
Fragments arranged by size:
1527 730 611 464 400 190 164 93
91 74 62
BspEI T'CCGG_A
Cuts at: 3626 3626
Size: 4406
BspHI T'CATG_A
Cuts at: 3582 3582
Size: 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 264 842 1456 1670 1972 2353 264
Size: 578 614 214 302 381 2317
Fragments arranged by size:
2317 614 578 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2698 2752 4236 4401 50
Size: 2648 54 1484 165 55
Fragments arranged by size:
2648 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2318 2318
Size: 4406
BsrFI r'CCGG_y
Cuts at: 2404 2585 2794 2404
Size: 181 209 4016
Fragments arranged by size:
4016 209 181
BsrGI T'GTAC_A
Cuts at: 669 669
Size: 4406
BssSI C'ACGA_G
Cuts at: 2677 3035 2677
Size: 358 4048
BstAPI GCAn_nnn'nTGC
Cuts at: 417 1783 417
Size: 1366 3040
BstBI TT'CG_AA
Cuts at: 2767 2767
Size: 4406
BstXI CCAn_nnnn'nTGG
Cuts at: 308 308
Size: 4406
BstYI r'GATC_y
Cuts at: 1 303 764 1198 1431 1898 1921 2256 2502
Size: 302 461 434 233 467 23 335 246
Cuts at: 2502 3514 3600 3612 3958 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 461 449 434 346 335 302
246 233 86 23 12
BtgI C'CryG_G
Cuts at: 292 292
Size: 4406
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2356 2537 2700 2356
Size: 181 163 4062
Fragments arranged by size:
4062 181 163
BtsI GCAGTG_nn'
Cuts at: 1649 1649
Size: 4406
DraI TTT'AAA
Cuts at: 465 1405 465
Size: 940 3466
DraIII CAC_nnn'GTG
Cuts at: 1110 1110
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 1991 2165 2556 2583 3928 1991
Size: 174 391 27 1345 2469
Fragments arranged by size:
2469 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 115 2614 2924 3070 4216 115
Size: 2499 310 146 1146 305
Fragments arranged by size:
2499 1146 310 305 146
EcoICRI GAG'CTC
Cuts at: 1418 1418
Size: 4406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 206 295 416 499 794 1154 1177 1196
Size: 29 89 121 83 295 360 23 19
Cuts at: 1196 2231 2663 3410 3678 177
Size: 1035 432 747 268 905
Fragments arranged by size:
1035 905 747 432 360 295 268 121
89 83 29 23 19
EcoO109I rG'GnC_Cy
Cuts at: 297 697 698 1131 1545 297
Size: 400 1 433 414 3158
Fragments arranged by size:
3158 433 414 400 1
EcoRI G'AATT_C
Cuts at: 932 1410 932
Size: 478 3928
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4406
FspI TGC'GCA
Cuts at: 2187 2187
Size: 4406
HaeII r_GCGC'y
Cuts at: 1840 2088 3110 4089 1840
Size: 248 1022 979 2157
Fragments arranged by size:
2157 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 477 509 741 773 2248 2278 2280 2310 477
Size: 32 232 32 1475 30 2 30 2573
Fragments arranged by size:
2573 1475 232 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1445 1445
Size: 4406
HindIII A'AGCT_T
Cuts at: 577 641 893 577
Size: 64 252 4090
Fragments arranged by size:
4090 252 64
KasI G'GCGC_C
Cuts at: 1836 2084 1836
Size: 248 4158
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 868 3077 3261 868
Size: 2209 184 2013
Fragments arranged by size:
2209 2013 184
MscI TGG'CCA
Cuts at: 2167 2167
Size: 4406
MslI CAynn'nnrTG
Cuts at: 2395 2522 2395
Size: 127 4279
MspA1I CmG'CkG
Cuts at: 1517 1831 2191 3204 3449 1517
Size: 314 360 1013 245 2474
Fragments arranged by size:
2474 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2587 2587
Size: 4406
NarI GG'CG_CC
Cuts at: 1837 2085 1837
Size: 248 4158
NcoI C'CATG_G
Cuts at: 292 292
Size: 4406
NgoMIV G'CCGG_C
Cuts at: 2585 2585
Size: 4406
NspI r_CATG'y
Cuts at: 1249 1459 1959 2866 1249
Size: 210 500 907 2789
Fragments arranged by size:
2789 907 500 210
PasI CC'CwG_GG
Cuts at: 308 308
Size: 4406
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PfoI T'CCnGG_A
Cuts at: 352 352
Size: 4406
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 4374
PpuMI rG'GwC_Cy
Cuts at: 1131 1131
Size: 4406
PsiI TTA'TAA
Cuts at: 379 379
Size: 4406
PspOMI G'GGCC_C
Cuts at: 297 697 297
Size: 400 4006
PstI C_TGCA'G
Cuts at: 839 1453 2138 839
Size: 614 685 3107
Fragments arranged by size:
3107 685 614
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 1831 2191 1831
Size: 360 4046
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1420 1420
Size: 4406
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SfcI C'TryA_G
Cuts at: 33 835 1449 2134 3127 3318 33
Size: 802 614 685 993 191 1121
Fragments arranged by size:
1121 993 802 685 614 191
SfoI GGC'GCC
Cuts at: 1838 2086 1838
Size: 248 4158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 1428 1428
Size: 4406
SmlI C'TyrA_G
Cuts at: 1561 2476 2968 3230 1561
Size: 915 492 262 2737
Fragments arranged by size:
2737 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StyI C'CwwG_G
Cuts at: 177 292 1134 1540 1894 177
Size: 115 842 406 354 2689
Fragments arranged by size:
2689 842 406 354 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1000 1989 2122 2581 2790 1000
Size: 989 133 459 209 2616
Fragments arranged by size:
2616 989 459 209 133
TatI w'GTAC_w
Cuts at: 669 3921 669
Size: 3252 1154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4374
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1901 1901
Size: 4406
XmaI C'CCGG_G
Cuts at: 1426 1426
Size: 4406
XmnI GAAnn'nnTTC
Cuts at: 559 895 1063 1276 1305 559
Size: 336 168 213 29 3660
Fragments arranged by size:
3660 336 213 168 29
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI