Restriction map for
pF1KB7271
(Circular) (Six-base) MAPSORT of: pF1KB7271.seq Check: 4558 from: 1 to: 4598
pF1KB7271 4598 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 160 1214 1636 160
Size: 1054 422 3122
Fragments arranged by size:
3122 1054 422
Acc65I G'GTAC_C
Cuts at: 1614 1614
Size: 4598
AclI AA'CG_TT
Cuts at: 4438 4438
Size: 4598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2855 3602 3870 2423
Size: 432 747 268 3151
Fragments arranged by size:
3151 747 432 268
AflII C'TTAA_G
Cuts at: 1288 1288
Size: 4598
AflIII A'CryG_T
Cuts at: 1950 3054 4031 1950
Size: 1104 977 2517
Fragments arranged by size:
2517 1104 977
AgeI A'CCGG_T
Cuts at: 2986 2986
Size: 4598
AleI CACnn'nnGTG
Cuts at: 307 307
Size: 4598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 335 335
Size: 4598
AlwNI CAG_nnn'CTG
Cuts at: 678 1588 1903 3470 678
Size: 910 315 1567 1806
Fragments arranged by size:
1806 1567 910 315
ApaLI G'TGCA_C
Cuts at: 3368 3368
Size: 4598
ApoI r'AATT_y
Cuts at: 1602 3951 4207 1602
Size: 2349 256 1993
Fragments arranged by size:
2349 1993 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4598
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
AvaI C'yCGr_G
Cuts at: 1557 1618 1557
Size: 61 4537
BamHI G'GATC_C
Cuts at: 1623 1623
Size: 4598
BanI G'GyrC_C
Cuts at: 1478 1614 2028 2276 2311 1478
Size: 136 414 248 35 3765
Fragments arranged by size:
3765 414 248 136 35
BanII G_rGCy'C
Cuts at: 223 391 501 604 1098 1558 1567 1612 2642
Size: 168 110 103 494 460 9 45 1030
Cuts at: 2642 223
Size: 2179
Fragments arranged by size:
2179 1030 494 460 168 110 103 45
9
BbeI G_GCGC'C
Cuts at: 1482 2032 2280 1482
Size: 550 248 3800
Fragments arranged by size:
3800 550 248
BbsI GAAGACnn'nnnn_
Cuts at: 1270 1270
Size: 4598
BciVI GTATCCnnnnn_n'
Cuts at: 2496 3257 4598 2496
Size: 761 1341 2496
Fragments arranged by size:
2496 1341 761
BclI T'GATC_A
Cuts at: 2118 2118
Size: 4598
BglI GCCn_nnn'nGGC
Cuts at: 490 490
Size: 4598
BglII A'GATC_T
Cuts at: 2113 4150 2113
Size: 2037 2561
BlpI GC'TnA_GC
Cuts at: 1710 1710
Size: 4598
Bme1580I G_kGCm'C
Cuts at: 548 1426 2223 2316 3372 548
Size: 878 797 93 1056 1774
Fragments arranged by size:
1774 1056 878 797 93
BmrI ACTGGGnnnn_n'
Cuts at: 602 972 1810 1891 2226 4370 602
Size: 370 838 81 335 2144 830
Fragments arranged by size:
2144 838 830 370 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 4598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 457 617 1007 1157 151
Size: 306 160 390 150 3592
Fragments arranged by size:
3592 390 306 160 150
Bpu10I CC'TnA_GC
Cuts at: 1013 1013
Size: 4598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1774 2653 3145 3443 1774
Size: 879 492 298 2929
Fragments arranged by size:
2929 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 993 4302 993
Size: 3309 1289
BsaAI yAC'GTr
Cuts at: 1951 1951
Size: 4598
BsaBI GATnn'nnATC
Cuts at: 6 2112 4149 6
Size: 2106 2037 455
Fragments arranged by size:
2106 2037 455
BsaHI Gr'CG_yC
Cuts at: 1479 2029 2277 2979 4475 1479
Size: 550 248 702 1496 1602
Fragments arranged by size:
1602 1496 702 550 248
BsaWI w'CCGG_w
Cuts at: 1787 2308 2986 3260 3407 3818 1787
Size: 521 678 274 147 411 2567
Fragments arranged by size:
2567 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1399 1429 1399
Size: 30 4568
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 368 371 368
Size: 3 4595
BseYI C'CCAG_C
Cuts at: 528 547 1337 2023 2781 3358 3854 528
Size: 19 790 686 758 577 496 1272
Fragments arranged by size:
1272 790 758 686 577 496 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 839 1139 839
Size: 300 4298
BsiEI CG_ry'CG
Cuts at: 104 2140 2186 3394 104
Size: 2036 46 1208 1308
Fragments arranged by size:
2036 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 223 501 1612 2390 2580 3372 223
Size: 278 1111 778 190 792 1449
Fragments arranged by size:
1449 1111 792 778 278 190
Bsp1286I G_dGCh'C
Cuts at: 223 391 501 548 604 1098 1426 1558 1567
Size: 168 110 47 56 494 328 132 9
Cuts at: 1567 1612 2223 2316 2390 2580 2642 3372 223
Size: 45 611 93 74 190 62 730 1449
Fragments arranged by size:
1449 730 611 494 328 190 168 132
110 93 74 62 56 47 45 9
BspEI T'CCGG_A
Cuts at: 3818 3818
Size: 4598
BspHI T'CATG_A
Cuts at: 3774 3774
Size: 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 866 1059 1648 1862 2164 2545 866
Size: 193 589 214 302 381 2919
Fragments arranged by size:
2919 589 381 302 214 193
BsrBI CCG'CTC
Cuts at: 50 506 2890 2944 4428 4593 50
Size: 456 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 456 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2510 2510
Size: 4598
BsrFI r'CCGG_y
Cuts at: 641 2596 2777 2986 641
Size: 1955 181 209 2253
Fragments arranged by size:
2253 1955 209 181
BsrGI T'GTAC_A
Cuts at: 454 454
Size: 4598
BssSI C'ACGA_G
Cuts at: 397 2869 3227 397
Size: 2472 358 1768
Fragments arranged by size:
2472 1768 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1975 1975
Size: 4598
BstBI TT'CG_AA
Cuts at: 2959 2959
Size: 4598
BstEII G'GTnAC_C
Cuts at: 247 247
Size: 4598
BstXI CCAn_nnnn'nTGG
Cuts at: 1131 1131
Size: 4598
BstYI r'GATC_y
Cuts at: 1 235 1499 1623 2090 2113 2448 2694 3706
Size: 234 1264 124 467 23 335 246 1012
Cuts at: 3706 3792 3804 4150 1
Size: 86 12 346 449
Fragments arranged by size:
1264 1012 467 449 346 335 246 234
124 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1061 1208 1061
Size: 147 4451
BtgI C'CryG_G
Cuts at: 107 461 107
Size: 354 4244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2548 2729 2892 2548
Size: 181 163 4254
Fragments arranged by size:
4254 181 163
BtsI GCAGTG_nn'
Cuts at: 1458 1841 1458
Size: 383 4215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 753 788 753
Size: 35 4563
DraI TTT'AAA
Cuts at: 1597 1597
Size: 4598
DrdI GACnn_nn'nnGTC
Cuts at: 2304 3162 2304
Size: 858 3740
EaeI y'GGCC_r
Cuts at: 491 2183 2357 2748 2775 4120 491
Size: 1692 174 391 27 1345 969
Fragments arranged by size:
1692 1345 969 391 174 27
EagI C'GGCC_G
Cuts at: 2183 2183
Size: 4598
EarI CTCTTCn'nnn_
Cuts at: 662 2621 2831 662
Size: 1959 210 2429
Fragments arranged by size:
2429 1959 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1094 2806 3116 3262 4408 1094
Size: 1712 310 146 1146 1284
Fragments arranged by size:
1712 1284 1146 310 146
EcoICRI GAG'CTC
Cuts at: 221 499 1610 221
Size: 278 1111 3209
Fragments arranged by size:
3209 1111 278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 457 617 1007 1157 2423 2855 3602 3870
Size: 306 160 390 150 1266 432 747 268
Cuts at: 3870 151
Size: 879
Fragments arranged by size:
1266 879 747 432 390 306 268 160
150
EcoNI CCTnn'n_nnAGG
Cuts at: 1012 1087 1012
Size: 75 4523
EcoO109I rG'GnC_Cy
Cuts at: 1048 1737 1048
Size: 689 3909
EcoRI G'AATT_C
Cuts at: 1602 1602
Size: 4598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 179 691 1287 2617 179
Size: 512 596 1330 2160
Fragments arranged by size:
2160 1330 596 512
FspI TGC'GCA
Cuts at: 1450 2379 1450
Size: 929 3669
HaeII r_GCGC'y
Cuts at: 1030 1482 2032 2280 3302 4281 1030
Size: 452 550 248 1022 979 1347
Fragments arranged by size:
1347 1022 979 550 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 378 410 1363 1395 2440 2470 2472 2502 378
Size: 32 953 32 1045 30 2 30 2474
Fragments arranged by size:
2474 1045 953 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1637 1637
Size: 4598
KasI G'GCGC_C
Cuts at: 1478 2028 2276 1478
Size: 550 248 3800
Fragments arranged by size:
3800 550 248
KpnI G_GTAC'C
Cuts at: 1618 1618
Size: 4598
MluI A'CGCG_T
Cuts at: 4031 4031
Size: 4598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 609 3269 3453 609
Size: 2660 184 1754
Fragments arranged by size:
2660 1754 184
MscI TGG'CCA
Cuts at: 493 2359 493
Size: 1866 2732
MslI CAynn'nnrTG
Cuts at: 307 317 2587 2714 307
Size: 10 2270 127 2191
Fragments arranged by size:
2270 2191 127 10
MspA1I CmG'CkG
Cuts at: 1337 1709 2023 2383 3396 3641 1337
Size: 372 314 360 1013 245 2294
Fragments arranged by size:
2294 1013 372 360 314 245
NaeI GCC'GGC
Cuts at: 643 2779 643
Size: 2136 2462
NarI GG'CG_CC
Cuts at: 1479 2029 2277 1479
Size: 550 248 3800
Fragments arranged by size:
3800 550 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4598
NdeI CA'TA_TG
Cuts at: 115 314 115
Size: 199 4399
NgoMIV G'CCGG_C
Cuts at: 641 2777 641
Size: 2136 2462
NspI r_CATG'y
Cuts at: 455 951 1474 1581 1651 2151 3058 455
Size: 496 523 107 70 500 907 1995
Fragments arranged by size:
1995 907 523 500 496 107 70
PasI CC'CwG_GG
Cuts at: 1089 1089
Size: 4598
PciI A'CATG_T
Cuts at: 3054 3054
Size: 4598
PfoI T'CCnGG_A
Cuts at: 128 128
Size: 4598
PmeI GTTT'AAAC
Cuts at: 1597 1597
Size: 4598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4566
PpuMI rG'GwC_Cy
Cuts at: 1048 1048
Size: 4598
PstI C_TGCA'G
Cuts at: 562 1336 1551 1645 2330 562
Size: 774 215 94 685 2830
Fragments arranged by size:
2830 774 685 215 94
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4598
PvuII CAG'CTG
Cuts at: 1337 2023 2383 1337
Size: 686 360 3552
Fragments arranged by size:
3552 686 360
RsrII CG'GwC_CG
Cuts at: 2793 2793
Size: 4598
SacI G_AGCT'C
Cuts at: 223 501 1612 223
Size: 278 1111 3209
Fragments arranged by size:
3209 1111 278
SalI G'TCGA_C
Cuts at: 1635 1635
Size: 4598
SapI GCTCTTCn'nnn_
Cuts at: 662 2621 2831 662
Size: 1959 210 2429
Fragments arranged by size:
2429 1959 210
SbfI CC_TGCA'GG
Cuts at: 562 1645 562
Size: 1083 3515
ScaI AGT'ACT
Cuts at: 4115 4115
Size: 4598
SfcI C'TryA_G
Cuts at: 33 558 1332 1547 1641 2326 3319 3510 33
Size: 525 774 215 94 685 993 191 1121
Fragments arranged by size:
1121 993 774 685 525 215 191 94
SfoI GGC'GCC
Cuts at: 1480 2030 2278 1480
Size: 550 248 3800
Fragments arranged by size:
3800 550 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
SmaI CCC'GGG
Cuts at: 1559 1620 1559
Size: 61 4537
SmlI C'TyrA_G
Cuts at: 1288 1753 2668 3160 3422 1288
Size: 465 915 492 262 2464
Fragments arranged by size:
2464 915 492 465 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4598
SphI G_CATG'C
Cuts at: 1474 1581 1651 2151 1474
Size: 107 70 500 3921
Fragments arranged by size:
3921 500 107 70
StyI C'CwwG_G
Cuts at: 107 566 1732 2086 107
Size: 459 1166 354 2619
Fragments arranged by size:
2619 1166 459 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1451 2181 2314 2773 2982 1451
Size: 730 133 459 209 3067
Fragments arranged by size:
3067 730 459 209 133
TatI w'GTAC_w
Cuts at: 454 4113 454
Size: 3659 939
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1040 1072 4115 4147 1040
Size: 32 3043 32 1491
Fragments arranged by size:
3043 1491 32 32
Tth111I GACn'n_nGTC
Cuts at: 2395 2395
Size: 4598
XbaI T'CTAG_A
Cuts at: 1629 1629
Size: 4598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 312 2093 312
Size: 1781 2817
XmaI C'CCGG_G
Cuts at: 1557 1618 1557
Size: 61 4537
XmnI GAAnn'nnTTC
Cuts at: 1324 1324
Size: 4598
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BssHII BstZ17I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI