Restriction map for
pF1KB7272
(Circular) (Six-base) MAPSORT of: pF1KB7272.seq Check: 2397 from: 1 to: 4439
pF1KB7272 4439 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 461 461
Size: 4439
AccI GT'mk_AC
Cuts at: 1477 1477
Size: 4439
Acc65I G'GTAC_C
Cuts at: 1455 1455
Size: 4439
AclI AA'CG_TT
Cuts at: 4279 4279
Size: 4439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 766 899 2264 2696 3049 3711 397
Size: 369 133 1365 432 353 662 1125
Fragments arranged by size:
1365 1125 662 432 369 353 133
AflIII A'CryG_T
Cuts at: 1791 3591 3872 1791
Size: 1800 281 2358
Fragments arranged by size:
2358 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 469 469
Size: 4439
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 721 753 721
Size: 32 4407
AlwNI CAG_nnn'CTG
Cuts at: 240 320 673 1083 1744 3182 240
Size: 80 353 410 661 1438 1497
Fragments arranged by size:
1497 1438 661 410 353 80
ApaI G_GGCC'C
Cuts at: 205 653 205
Size: 448 3991
ApaLI G'TGCA_C
Cuts at: 1360 3277 1360
Size: 1917 2522
ApoI r'AATT_y
Cuts at: 510 1073 1443 3792 4048 510
Size: 563 370 2349 256 901
Fragments arranged by size:
2349 901 563 370 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
AvaI C'yCGr_G
Cuts at: 1367 1459 1367
Size: 92 4347
BamHI G'GATC_C
Cuts at: 1464 1464
Size: 4439
BanI G'GyrC_C
Cuts at: 173 1455 1869 2117 2152 173
Size: 1282 414 248 35 2460
Fragments arranged by size:
2460 1282 414 248 35
BanII G_rGCy'C
Cuts at: 205 653 765 953 1453 2483 205
Size: 448 112 188 500 1030 2161
Fragments arranged by size:
2161 1030 500 448 188 112
BbeI G_GCGC'C
Cuts at: 1873 2121 1873
Size: 248 4191
BbvCI CC'TCA_GC
Cuts at: 1354 1354
Size: 4439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 612 646 90
Size: 34 488 34 3883
Fragments arranged by size:
3883 488 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2337 3393 4439 2337
Size: 1056 1046 2337
Fragments arranged by size:
2337 1056 1046
BclI T'GATC_A
Cuts at: 1959 1959
Size: 4439
BglI GCCn_nnn'nGGC
Cuts at: 112 142 112
Size: 30 4409
BglII A'GATC_T
Cuts at: 1954 3991 1954
Size: 2037 2402
BlpI GC'TnA_GC
Cuts at: 241 1551 241
Size: 1310 3129
Bme1580I G_kGCm'C
Cuts at: 205 653 1364 2064 2157 3281 205
Size: 448 711 700 93 1124 1363
Fragments arranged by size:
1363 1124 711 700 448 93
BmrI ACTGGGnnnn_n'
Cuts at: 249 504 1651 1732 2067 4211 249
Size: 255 1147 81 335 2144 477
Fragments arranged by size:
2144 1147 477 335 255 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1061 1203 1061
Size: 142 4297
Bpu10I CC'TnA_GC
Cuts at: 674 1354 674
Size: 680 3759
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 2494 3208 3506 1615
Size: 879 714 298 2548
Fragments arranged by size:
2548 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1051 4143 1051
Size: 3092 1347
BsaAI yAC'GTr
Cuts at: 1792 1792
Size: 4439
BsaBI GATnn'nnATC
Cuts at: 6 1953 3990 6
Size: 1947 2037 455
Fragments arranged by size:
2037 1947 455
BsaHI Gr'CG_yC
Cuts at: 1870 2118 2820 4316 1870
Size: 248 702 1496 1993
Fragments arranged by size:
1993 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1628 2149 2827 3238 3385 3659 1628
Size: 521 678 411 147 274 2408
Fragments arranged by size:
2408 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 721 751 721
Size: 30 4409
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1147 1264 1147
Size: 117 4322
BseYI C'CCAG_C
Cuts at: 1326 1864 2622 3287 3695 1326
Size: 538 758 665 408 2070
Fragments arranged by size:
2070 758 665 538 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 693 693
Size: 4439
BsiEI CG_ry'CG
Cuts at: 104 137 264 1981 2027 3257 104
Size: 33 127 1717 46 1230 1286
Fragments arranged by size:
1717 1286 1230 127 46 33
BsiHKAI G_wGCw'C
Cuts at: 247 765 1364 1453 2231 2421 3281 247
Size: 518 599 89 778 190 860 1405
Fragments arranged by size:
1405 860 778 599 518 190 89
BsmBI CGTCTCn'nnnn_
Cuts at: 255 284 255
Size: 29 4410
Bsp1286I G_dGCh'C
Cuts at: 205 247 653 765 953 1364 1453 2064 2157
Size: 42 406 112 188 411 89 611 93
Cuts at: 2157 2231 2421 2483 3281 205
Size: 74 190 62 798 1363
Fragments arranged by size:
1363 798 611 411 406 190 188 112
93 89 74 62 42
BspHI T'CATG_A
Cuts at: 2871 2871
Size: 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 812 1489 1703 2005 2386 461
Size: 351 677 214 302 381 2514
Fragments arranged by size:
2514 677 381 351 302 214
BsrBI CCG'CTC
Cuts at: 50 2731 2785 4269 4434 50
Size: 2681 54 1484 165 55
Fragments arranged by size:
2681 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2351 2351
Size: 4439
BsrFI r'CCGG_y
Cuts at: 111 170 2437 2618 111
Size: 59 2267 181 1932
Fragments arranged by size:
2267 1932 181 59
BssSI C'ACGA_G
Cuts at: 769 975 2710 3418 769
Size: 206 1735 708 1790
Fragments arranged by size:
1790 1735 708 206
BstAPI GCAn_nnn'nTGC
Cuts at: 1816 1816
Size: 4439
BstBI TT'CG_AA
Cuts at: 508 2800 508
Size: 2292 2147
BstXI CCAn_nnnn'nTGG
Cuts at: 936 1039 936
Size: 103 4336
BstYI r'GATC_y
Cuts at: 1 1464 1931 1954 2289 2535 2841 2853 2939
Size: 1463 467 23 335 246 306 12 86
Cuts at: 2939 3991 1
Size: 1052 449
Fragments arranged by size:
1463 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1263 1263
Size: 4439
BtgI C'CryG_G
Cuts at: 140 210 536 551 1161 1289 140
Size: 70 326 15 610 128 3290
Fragments arranged by size:
3290 610 326 128 70 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2389 2570 2733 2389
Size: 181 163 4095
Fragments arranged by size:
4095 181 163
BtsI GCAGTG_nn'
Cuts at: 1183 1284 1682 1183
Size: 101 398 3940
Fragments arranged by size:
3940 398 101
DraI TTT'AAA
Cuts at: 1438 1438
Size: 4439
DraIII CAC_nnn'GTG
Cuts at: 402 1157 402
Size: 755 3684
DrdI GACnn_nn'nnGTC
Cuts at: 2145 3489 2145
Size: 1344 3095
EaeI y'GGCC_r
Cuts at: 134 570 2024 2198 2589 2616 3961 134
Size: 436 1454 174 391 27 1345 612
Fragments arranged by size:
1454 1345 612 436 391 174 27
EagI C'GGCC_G
Cuts at: 134 2024 134
Size: 1890 2549
EarI CTCTTCn'nnn_
Cuts at: 1241 2462 2672 1241
Size: 1221 210 3008
Fragments arranged by size:
3008 1221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2647 3389 3535 4249 2647
Size: 742 146 714 2837
Fragments arranged by size:
2837 742 714 146
EcoICRI GAG'CTC
Cuts at: 763 1451 763
Size: 688 3751
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 766 899 1061 1203 2264 2696 3049 3711
Size: 369 133 162 142 1061 432 353 662
Cuts at: 3711 397
Size: 1125
Fragments arranged by size:
1125 1061 662 432 369 353 162 142
133
EcoO109I rG'GnC_Cy
Cuts at: 202 366 650 1109 1578 202
Size: 164 284 459 469 3063
Fragments arranged by size:
3063 469 459 284 164
EcoRI G'AATT_C
Cuts at: 1073 1443 1073
Size: 370 4069
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4439
FspI TGC'GCA
Cuts at: 2220 2220
Size: 4439
HaeII r_GCGC'y
Cuts at: 1873 2121 3351 4122 1873
Size: 248 1230 771 2190
Fragments arranged by size:
2190 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 559 591 2281 2311 2313 2343 559
Size: 32 1690 30 2 30 2655
Fragments arranged by size:
2655 1690 32 30 30 2
HincII GTy'rAC
Cuts at: 1478 1478
Size: 4439
HindIII A'AGCT_T
Cuts at: 1000 1000
Size: 4439
KasI G'GCGC_C
Cuts at: 1869 2117 1869
Size: 248 4191
KpnI G_GTAC'C
Cuts at: 1459 1459
Size: 4439
MluI A'CGCG_T
Cuts at: 3872 3872
Size: 4439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 480 830 863 3198 3382 480
Size: 350 33 2335 184 1537
Fragments arranged by size:
2335 1537 350 184 33
MscI TGG'CCA
Cuts at: 572 2200 572
Size: 1628 2811
MslI CAynn'nnrTG
Cuts at: 588 773 2428 2555 588
Size: 185 1655 127 2472
Fragments arranged by size:
2472 1655 185 127
MspA1I CmG'CkG
Cuts at: 142 1550 1864 2224 3008 3253 142
Size: 1408 314 360 784 245 1328
Fragments arranged by size:
1408 1328 784 360 314 245
NaeI GCC'GGC
Cuts at: 113 172 2620 113
Size: 59 2448 1932
Fragments arranged by size:
2448 1932 59
NarI GG'CG_CC
Cuts at: 1870 2118 1870
Size: 248 4191
NcoI C'CATG_G
Cuts at: 551 551
Size: 4439
NdeI CA'TA_TG
Cuts at: 1429 1429
Size: 4439
NgoMIV G'CCGG_C
Cuts at: 111 170 2618 111
Size: 59 2448 1932
Fragments arranged by size:
2448 1932 59
NotI GC'GGCC_GC
Cuts at: 134 134
Size: 4439
NspI r_CATG'y
Cuts at: 1492 1992 3595 1492
Size: 500 1603 2336
Fragments arranged by size:
2336 1603 500
PasI CC'CwG_GG
Cuts at: 861 861
Size: 4439
PciI A'CATG_T
Cuts at: 3591 3591
Size: 4439
PflMI CCAn_nnn'nTGG
Cuts at: 351 351
Size: 4439
PfoI T'CCnGG_A
Cuts at: 1219 1219
Size: 4439
PmeI GTTT'AAAC
Cuts at: 1438 1438
Size: 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 721 753 2885 2917 721
Size: 32 2132 32 2243
Fragments arranged by size:
2243 2132 32 32
PpuMI rG'GwC_Cy
Cuts at: 366 366
Size: 4439
PspOMI G'GGCC_C
Cuts at: 201 649 201
Size: 448 3991
PstI C_TGCA'G
Cuts at: 1486 2171 1486
Size: 685 3754
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4439
PvuII CAG'CTG
Cuts at: 1864 2224 1864
Size: 360 4079
RsrII CG'GwC_CG
Cuts at: 2634 2634
Size: 4439
SacI G_AGCT'C
Cuts at: 765 1453 765
Size: 688 3751
SacII CC_GC'GG
Cuts at: 143 143
Size: 4439
SalI G'TCGA_C
Cuts at: 1476 1476
Size: 4439
SapI GCTCTTCn'nnn_
Cuts at: 2462 2672 2462
Size: 210 4229
SbfI CC_TGCA'GG
Cuts at: 1486 1486
Size: 4439
ScaI AGT'ACT
Cuts at: 274 3956 274
Size: 3682 757
SfcI C'TryA_G
Cuts at: 33 1482 2167 3135 3326 33
Size: 1449 685 968 191 1146
Fragments arranged by size:
1449 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1871 2119 1871
Size: 248 4191
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
SmaI CCC'GGG
Cuts at: 1461 1461
Size: 4439
SmlI C'TyrA_G
Cuts at: 1594 2509 3223 3485 1594
Size: 915 714 262 2548
Fragments arranged by size:
2548 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4439
SphI G_CATG'C
Cuts at: 1492 1992 1492
Size: 500 3939
StyI C'CwwG_G
Cuts at: 362 551 816 1573 1927 362
Size: 189 265 757 354 2874
Fragments arranged by size:
2874 757 354 265 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2022 2155 2614 2823 2022
Size: 133 459 209 3638
Fragments arranged by size:
3638 459 209 133
TatI w'GTAC_w
Cuts at: 272 3954 272
Size: 3682 757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1215 1247 3956 3988 1215
Size: 32 2709 32 1666
Fragments arranged by size:
2709 1666 32 32
Tth111I GACn'n_nGTC
Cuts at: 2236 2236
Size: 4439
XbaI T'CTAG_A
Cuts at: 1470 1470
Size: 4439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 594 1934 594
Size: 1340 3099
XmaI C'CCGG_G
Cuts at: 1459 1459
Size: 4439
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI BspEI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NheI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI