Restriction map for
pF1KB7274
(Circular) (Six-base) MAPSORT of: pF1KB7274.seq Check: 7687 from: 1 to: 5348
pF1KB7274 5348 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 2386 2386
Size: 5348
Acc65I G'GTAC_C
Cuts at: 1034 2364 1034
Size: 1330 4018
AclI AA'CG_TT
Cuts at: 5188 5188
Size: 5348
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 750 998 1597 1850 1976 1994 3173 3605
Size: 85 248 599 253 126 18 1179 432
Cuts at: 3605 4352 4620 665
Size: 747 268 1393
Fragments arranged by size:
1393 1179 747 599 432 268 253 248
126 85 18
AflIII A'CryG_T
Cuts at: 474 1963 2700 3804 4781 474
Size: 1489 737 1104 977 1041
Fragments arranged by size:
1489 1104 1041 977 737
AgeI A'CCGG_T
Cuts at: 3736 3736
Size: 5348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 891 1726 891
Size: 835 4513
AlwNI CAG_nnn'CTG
Cuts at: 1807 2653 4220 1807
Size: 846 1567 2935
Fragments arranged by size:
2935 1567 846
ApaLI G'TGCA_C
Cuts at: 1247 4118 1247
Size: 2871 2477
ApoI r'AATT_y
Cuts at: 395 681 734 919 1157 1333 1402 1851 2316
Size: 286 53 185 238 176 69 449 465
Cuts at: 2316 2352 4701 4957 395
Size: 36 2349 256 786
Fragments arranged by size:
2349 786 465 449 286 256 238 185
176 69 53 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 5348
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5348
AvaI C'yCGr_G
Cuts at: 2368 2368
Size: 5348
BamHI G'GATC_C
Cuts at: 2373 2373
Size: 5348
BanI G'GyrC_C
Cuts at: 1034 2364 2778 3026 3061 1034
Size: 1330 414 248 35 3321
Fragments arranged by size:
3321 1330 414 248 35
BanII G_rGCy'C
Cuts at: 2362 3392 2362
Size: 1030 4318
BbeI G_GCGC'C
Cuts at: 2782 3030 2782
Size: 248 5100
BbsI GAAGACnn'nnnn_
Cuts at: 1741 1741
Size: 5348
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5314
BciVI GTATCCnnnnn_n'
Cuts at: 326 3246 4007 5348 326
Size: 2920 761 1341 326
Fragments arranged by size:
2920 1341 761 326
BclI T'GATC_A
Cuts at: 2868 2868
Size: 5348
BfrBI ATG'CAT
Cuts at: 1566 1566
Size: 5348
BglII A'GATC_T
Cuts at: 1987 2863 4900 1987
Size: 876 2037 2435
Fragments arranged by size:
2435 2037 876
BlpI GC'TnA_GC
Cuts at: 189 2460 189
Size: 2271 3077
Bme1580I G_kGCm'C
Cuts at: 1251 2973 3066 4122 1251
Size: 1722 93 1056 2477
Fragments arranged by size:
2477 1722 1056 93
BmgBI CAC'GTC
Cuts at: 1966 1966
Size: 5348
BmrI ACTGGGnnnn_n'
Cuts at: 1019 1356 2560 2641 2976 5120 1019
Size: 337 1204 81 335 2144 1247
Fragments arranged by size:
2144 1247 1204 337 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3715 3715
Size: 5348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1772 1929 1772
Size: 157 5191
Bpu10I CC'TnA_GC
Cuts at: 1866 1866
Size: 5348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2524 3403 3895 4193 2524
Size: 879 492 298 3679
Fragments arranged by size:
3679 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1947 5052 1947
Size: 3105 2243
BsaAI yAC'GTr
Cuts at: 1085 2701 1085
Size: 1616 3732
BsaBI GATnn'nnATC
Cuts at: 6 653 2862 4899 6
Size: 647 2209 2037 455
Fragments arranged by size:
2209 2037 647 455
BsaHI Gr'CG_yC
Cuts at: 2779 3027 3729 5225 2779
Size: 248 702 1496 2902
Fragments arranged by size:
2902 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2537 3058 3736 4010 4157 4568 2537
Size: 521 678 274 147 411 3317
Fragments arranged by size:
3317 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1138 1138
Size: 5348
BseYI C'CCAG_C
Cuts at: 2773 3531 4108 4604 2773
Size: 758 577 496 3517
Fragments arranged by size:
3517 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2890 2936 4144 104
Size: 2786 46 1208 1308
Fragments arranged by size:
2786 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1251 2362 3140 3330 4122 1251
Size: 1111 778 190 792 2477
Fragments arranged by size:
2477 1111 792 778 190
BsmI GAATG_Cn'
Cuts at: 562 988 1629 1889 562
Size: 426 641 260 4021
Fragments arranged by size:
4021 641 426 260
Bsp1286I G_dGCh'C
Cuts at: 1251 2362 2973 3066 3140 3330 3392 4122 1251
Size: 1111 611 93 74 190 62 730 2477
Fragments arranged by size:
2477 1111 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4568 4568
Size: 5348
BspHI T'CATG_A
Cuts at: 4524 4524
Size: 5348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2398 2612 2914 3295 2398
Size: 214 302 381 4451
Fragments arranged by size:
4451 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3640 3694 5178 5343 50
Size: 3590 54 1484 165 55
Fragments arranged by size:
3590 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1568 3260 1568
Size: 1692 3656
BsrFI r'CCGG_y
Cuts at: 3346 3527 3736 3346
Size: 181 209 4958
Fragments arranged by size:
4958 209 181
BsrGI T'GTAC_A
Cuts at: 912 912
Size: 5348
BssSI C'ACGA_G
Cuts at: 3619 3977 3619
Size: 358 4990
BstAPI GCAn_nnn'nTGC
Cuts at: 2725 2725
Size: 5348
BstBI TT'CG_AA
Cuts at: 3709 3709
Size: 5348
BstEII G'GTnAC_C
Cuts at: 723 723
Size: 5348
BstYI r'GATC_y
Cuts at: 1 409 654 1987 2373 2840 2863 3198 3444
Size: 408 245 1333 386 467 23 335 246
Cuts at: 3444 4456 4542 4554 4900 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1333 1012 467 449 408 386 346 335
246 245 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3298 3479 3642 3298
Size: 181 163 5004
Fragments arranged by size:
5004 181 163
BtsI GCAGTG_nn'
Cuts at: 628 2591 628
Size: 1963 3385
DraI TTT'AAA
Cuts at: 1437 2347 1437
Size: 910 4438
DraIII CAC_nnn'GTG
Cuts at: 1415 1415
Size: 5348
DrdI GACnn_nn'nnGTC
Cuts at: 3054 3912 3054
Size: 858 4490
EaeI y'GGCC_r
Cuts at: 949 2933 3107 3498 3525 4870 949
Size: 1984 174 391 27 1345 1427
Fragments arranged by size:
1984 1427 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2933 2933
Size: 5348
EarI CTCTTCn'nnn_
Cuts at: 260 266 551 2075 2102 3371 3581 260
Size: 6 285 1524 27 1269 210 2027
Fragments arranged by size:
2027 1524 1269 285 210 27 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3556 3866 4012 5158 3556
Size: 310 146 1146 3746
Fragments arranged by size:
3746 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2360 2360
Size: 5348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 750 998 1597 1772 1850 1929 1976 1994
Size: 85 248 599 175 78 79 47 18
Cuts at: 1994 3173 3605 4352 4620 665
Size: 1179 432 747 268 1393
Fragments arranged by size:
1393 1179 747 599 432 268 248 175
85 79 78 47 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2045
Size: 5348
EcoO109I rG'GnC_Cy
Cuts at: 1008 2487 1008
Size: 1479 3869
EcoRI G'AATT_C
Cuts at: 2316 2352 2316
Size: 36 5312
EcoRV GAT'ATC
Cuts at: 2277 2277
Size: 5348
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3367 3367
Size: 5348
FspI TGC'GCA
Cuts at: 3129 3129
Size: 5348
HaeII r_GCGC'y
Cuts at: 2782 3030 4052 5031 2782
Size: 248 1022 979 3099
Fragments arranged by size:
3099 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1474 1506 2322 2354 3190 3220 3222 3252 1474
Size: 32 816 32 836 30 2 30 3570
Fragments arranged by size:
3570 836 816 32 32 30 30 2
HincII GTy'rAC
Cuts at: 773 2387 773
Size: 1614 3734
HindIII A'AGCT_T
Cuts at: 1317 1317
Size: 5348
HpaI GTT'AAC
Cuts at: 773 773
Size: 5348
KasI G'GCGC_C
Cuts at: 2778 3026 2778
Size: 248 5100
KpnI G_GTAC'C
Cuts at: 1038 2368 1038
Size: 1330 4018
MluI A'CGCG_T
Cuts at: 4781 4781
Size: 5348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 366 1293 4019 4203 366
Size: 927 2726 184 1511
Fragments arranged by size:
2726 1511 927 184
MscI TGG'CCA
Cuts at: 951 3109 951
Size: 2158 3190
MslI CAynn'nnrTG
Cuts at: 1708 3337 3464 1708
Size: 1629 127 3592
Fragments arranged by size:
3592 1629 127
MspA1I CmG'CkG
Cuts at: 1792 2227 2459 2773 3133 4146 4391 1792
Size: 435 232 314 360 1013 245 2749
Fragments arranged by size:
2749 1013 435 360 314 245 232
NaeI GCC'GGC
Cuts at: 3529 3529
Size: 5348
NarI GG'CG_CC
Cuts at: 2779 3027 2779
Size: 248 5100
NgoMIV G'CCGG_C
Cuts at: 3527 3527
Size: 5348
NsiI A_TGCA'T
Cuts at: 1568 1568
Size: 5348
NspI r_CATG'y
Cuts at: 478 1570 1707 2401 2901 3808 478
Size: 1092 137 694 500 907 2018
Fragments arranged by size:
2018 1092 907 694 500 137
PciI A'CATG_T
Cuts at: 474 3804 474
Size: 3330 2018
PmeI GTTT'AAAC
Cuts at: 2347 2347
Size: 5348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4487 4519 4487
Size: 32 5316
PpuMI rG'GwC_Cy
Cuts at: 1008 1008
Size: 5348
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1069 1101 1069
Size: 32 5316
PstI C_TGCA'G
Cuts at: 284 635 2395 3080 284
Size: 351 1760 685 2552
Fragments arranged by size:
2552 1760 685 351
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5348
PvuII CAG'CTG
Cuts at: 1792 2773 3133 1792
Size: 981 360 4007
Fragments arranged by size:
4007 981 360
RsrII CG'GwC_CG
Cuts at: 3543 3543
Size: 5348
SacI G_AGCT'C
Cuts at: 2362 2362
Size: 5348
SalI G'TCGA_C
Cuts at: 2385 2385
Size: 5348
SanDI GG'GwC_CC
Cuts at: 1008 1008
Size: 5348
SapI GCTCTTCn'nnn_
Cuts at: 3371 3581 3371
Size: 210 5138
SbfI CC_TGCA'GG
Cuts at: 2395 2395
Size: 5348
ScaI AGT'ACT
Cuts at: 4865 4865
Size: 5348
SexAI A'CCwGG_T
Cuts at: 317 317
Size: 5348
SfcI C'TryA_G
Cuts at: 33 280 631 2042 2391 3076 4069 4260 33
Size: 247 351 1411 349 685 993 191 1121
Fragments arranged by size:
1411 1121 993 685 351 349 247 191
SfoI GGC'GCC
Cuts at: 2780 3028 2780
Size: 248 5100
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5348
SmaI CCC'GGG
Cuts at: 2370 2370
Size: 5348
SmlI C'TyrA_G
Cuts at: 2503 3418 3910 4172 2503
Size: 915 492 262 3679
Fragments arranged by size:
3679 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1723 67
Size: 1656 3692
SphI G_CATG'C
Cuts at: 2401 2901 2401
Size: 500 4848
StyI C'CwwG_G
Cuts at: 200 1012 2482 2836 200
Size: 812 1470 354 2712
Fragments arranged by size:
2712 1470 812 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1266 1392 1407 2931 3064 3523 3732 1266
Size: 126 15 1524 133 459 209 2882
Fragments arranged by size:
2882 1524 459 209 133 126 15
TatI w'GTAC_w
Cuts at: 912 1217 1233 2197 2260 4863 912
Size: 305 16 964 63 2603 1397
Fragments arranged by size:
2603 1397 964 305 63 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4865 4897 4865
Size: 32 5316
Tth111I GACn'n_nGTC
Cuts at: 3145 3145
Size: 5348
XbaI T'CTAG_A
Cuts at: 2379 2379
Size: 5348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2843 2843
Size: 5348
XmaI C'CCGG_G
Cuts at: 2368 2368
Size: 5348
XmnI GAAnn'nnTTC
Cuts at: 1403 1889 2216 1403
Size: 486 327 4535
Fragments arranged by size:
4535 486 327
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmtI BsaXI BsgI
BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI FseI FspAI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI