Restriction map for
pF1KB7275
(Circular) (Six-base) MAPSORT of: pF1KB7275.seq Check: 5168 from: 1 to: 5738
pF1KB7275 5738 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1087 1087
Size: 5738
AccI GT'mk_AC
Cuts at: 2776 2776
Size: 5738
Acc65I G'GTAC_C
Cuts at: 2250 2754 2250
Size: 504 5234
AclI AA'CG_TT
Cuts at: 5578 5578
Size: 5738
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 1649 1756 2585 3563 3995 4348 5010 1195
Size: 454 107 829 978 432 353 662 1923
Fragments arranged by size:
1923 978 829 662 454 432 353 107
AflII C'TTAA_G
Cuts at: 160 160
Size: 5738
AflIII A'CryG_T
Cuts at: 673 1298 3090 4890 5171 673
Size: 625 1792 1800 281 1240
Fragments arranged by size:
1800 1792 1240 625 281
AleI CACnn'nnGTG
Cuts at: 373 1196 373
Size: 823 4915
AlwNI CAG_nnn'CTG
Cuts at: 1509 3043 4481 1509
Size: 1534 1438 2766
Fragments arranged by size:
2766 1534 1438
ApaLI G'TGCA_C
Cuts at: 4576 4576
Size: 5738
ApoI r'AATT_y
Cuts at: 406 680 813 1185 1926 2266 2687 2742 5091
Size: 274 133 372 741 340 421 55 2349
Cuts at: 5091 5347 406
Size: 256 797
Fragments arranged by size:
2349 797 741 421 372 340 274 256
133 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 5738
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5738
AvaI C'yCGr_G
Cuts at: 2618 2758 2618
Size: 140 5598
AvrII C'CTAG_G
Cuts at: 1945 1945
Size: 5738
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2242 2275 2242
Size: 33 5705
BamHI G'GATC_C
Cuts at: 2763 2763
Size: 5738
BanI G'GyrC_C
Cuts at: 1740 2250 2754 3168 3416 3451 1740
Size: 510 504 414 248 35 4027
Fragments arranged by size:
4027 510 504 414 248 35
BanII G_rGCy'C
Cuts at: 1332 2752 3782 1332
Size: 1420 1030 3288
Fragments arranged by size:
3288 1420 1030
BbeI G_GCGC'C
Cuts at: 3172 3420 3172
Size: 248 5490
BbsI GAAGACnn'nnnn_
Cuts at: 371 1630 371
Size: 1259 4479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 329 363 1294 1328 329
Size: 34 931 34 4739
Fragments arranged by size:
4739 931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 633 719 767 3636 4692 5738 633
Size: 86 48 2869 1056 1046 633
Fragments arranged by size:
2869 1056 1046 633 86 48
BclI T'GATC_A
Cuts at: 206 1404 3258 206
Size: 1198 1854 2686
Fragments arranged by size:
2686 1854 1198
BfrBI ATG'CAT
Cuts at: 289 289
Size: 5738
BglII A'GATC_T
Cuts at: 3253 5290 3253
Size: 2037 3701
BlpI GC'TnA_GC
Cuts at: 2850 2850
Size: 5738
Bme1580I G_kGCm'C
Cuts at: 565 3363 3456 4580 565
Size: 2798 93 1124 1723
Fragments arranged by size:
2798 1723 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1604 2049 2654 2950 3031 3366 5510 1604
Size: 445 605 296 81 335 2144 1832
Fragments arranged by size:
2144 1832 605 445 335 296 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4105 4105
Size: 5738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 2375 325
Size: 2050 3688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2914 3793 4507 4805 2914
Size: 879 714 298 3847
Fragments arranged by size:
3847 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 415 5442 415
Size: 5027 711
BsaAI yAC'GTr
Cuts at: 3091 3091
Size: 5738
BsaBI GATnn'nnATC
Cuts at: 6 800 3252 5289 6
Size: 794 2452 2037 455
Fragments arranged by size:
2452 2037 794 455
BsaHI Gr'CG_yC
Cuts at: 3169 3417 4119 5615 3169
Size: 248 702 1496 3292
Fragments arranged by size:
3292 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2487 2927 3448 4126 4537 4684 4958 2487
Size: 440 521 678 411 147 274 3267
Fragments arranged by size:
3267 678 521 440 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2324 2354 2324
Size: 30 5708
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1559 1783 1559
Size: 224 5514
BseYI C'CCAG_C
Cuts at: 353 548 3163 3921 4586 4994 353
Size: 195 2615 758 665 408 1097
Fragments arranged by size:
2615 1097 758 665 408 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 2692 1435
Size: 1257 4481
BsiEI CG_ry'CG
Cuts at: 104 3280 3326 4556 104
Size: 3176 46 1230 1286
Fragments arranged by size:
3176 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1332 2752 3530 3720 4580 1332
Size: 1420 778 190 860 2490
Fragments arranged by size:
2490 1420 860 778 190
BsmI GAATG_Cn'
Cuts at: 1685 1685
Size: 5738
Bsp1286I G_dGCh'C
Cuts at: 565 1332 2752 3363 3456 3530 3720 3782 4580
Size: 767 1420 611 93 74 190 62 798
Cuts at: 4580 565
Size: 1723
Fragments arranged by size:
1723 1420 798 767 611 190 93 74
62
BspHI T'CATG_A
Cuts at: 1344 4170 1344
Size: 2826 2912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 587 1037 1087 2788 3002 3304 3685 587
Size: 450 50 1701 214 302 381 2640
Fragments arranged by size:
2640 1701 450 381 302 214 50
BsrBI CCG'CTC
Cuts at: 50 4030 4084 5568 5733 50
Size: 3980 54 1484 165 55
Fragments arranged by size:
3980 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 890 1807 2612 3650 890
Size: 917 805 1038 2978
Fragments arranged by size:
2978 1038 917 805
BsrFI r'CCGG_y
Cuts at: 314 3736 3917 314
Size: 3422 181 2135
Fragments arranged by size:
3422 2135 181
BssSI C'ACGA_G
Cuts at: 4009 4717 4009
Size: 708 5030
BstAPI GCAn_nnn'nTGC
Cuts at: 3115 3115
Size: 5738
BstBI TT'CG_AA
Cuts at: 4099 4099
Size: 5738
BstXI CCAn_nnnn'nTGG
Cuts at: 854 854
Size: 5738
BstYI r'GATC_y
Cuts at: 1 2763 3230 3253 3588 3834 4140 4152 4238
Size: 2762 467 23 335 246 306 12 86
Cuts at: 4238 5290 1
Size: 1052 449
Fragments arranged by size:
2762 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1224 1554 1713 2175 107
Size: 1117 330 159 462 3670
Fragments arranged by size:
3670 1117 462 330 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2054 3688 3869 4032 2054
Size: 1634 181 163 3760
Fragments arranged by size:
3760 1634 181 163
BtsI GCAGTG_nn'
Cuts at: 2981 2981
Size: 5738
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 853 888 853
Size: 35 5703
DraI TTT'AAA
Cuts at: 899 1617 2737 899
Size: 718 1120 3900
Fragments arranged by size:
3900 1120 718
DrdI GACnn_nn'nnGTC
Cuts at: 3444 4788 3444
Size: 1344 4394
EaeI y'GGCC_r
Cuts at: 2376 3323 3497 3888 3915 5260 2376
Size: 947 174 391 27 1345 2854
Fragments arranged by size:
2854 1345 947 391 174 27
EagI C'GGCC_G
Cuts at: 3323 3323
Size: 5738
EarI CTCTTCn'nnn_
Cuts at: 989 3761 3971 989
Size: 2772 210 2756
Fragments arranged by size:
2772 2756 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3946 4688 4834 5548 3946
Size: 742 146 714 4136
Fragments arranged by size:
4136 742 714 146
EcoICRI GAG'CTC
Cuts at: 1330 2750 1330
Size: 1420 4318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1195 1649 1756 2375 2585 3563 3995 4348
Size: 870 454 107 619 210 978 432 353
Cuts at: 4348 5010 325
Size: 662 1053
Fragments arranged by size:
1053 978 870 662 619 454 432 353
210 107
EcoO109I rG'GnC_Cy
Cuts at: 1309 2877 1309
Size: 1568 4170
EcoRI G'AATT_C
Cuts at: 680 2266 2742 680
Size: 1586 476 3676
Fragments arranged by size:
3676 1586 476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 297 3757 297
Size: 3460 2278
FspI TGC'GCA
Cuts at: 3519 3519
Size: 5738
HaeII r_GCGC'y
Cuts at: 3172 3420 4650 5421 3172
Size: 248 1230 771 3489
Fragments arranged by size:
3489 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1759 1791 1872 1904 3580 3610 3612 3642 1759
Size: 32 81 32 1676 30 2 30 3855
Fragments arranged by size:
3855 1676 81 32 32 30 30 2
HincII GTy'rAC
Cuts at: 122 2777 122
Size: 2655 3083
KasI G'GCGC_C
Cuts at: 3168 3416 3168
Size: 248 5490
KpnI G_GTAC'C
Cuts at: 2254 2758 2254
Size: 504 5234
MluI A'CGCG_T
Cuts at: 5171 5171
Size: 5738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4497 4681 4497
Size: 184 5554
MscI TGG'CCA
Cuts at: 2378 3499 2378
Size: 1121 4617
MslI CAynn'nnrTG
Cuts at: 373 964 1097 1196 2384 3727 3854 373
Size: 591 133 99 1188 1343 127 2257
Fragments arranged by size:
2257 1343 1188 591 133 127 99
MspA1I CmG'CkG
Cuts at: 266 1182 1863 2849 3163 3523 4307 4552 266
Size: 916 681 986 314 360 784 245 1452
Fragments arranged by size:
1452 986 916 784 681 360 314 245
NaeI GCC'GGC
Cuts at: 3919 3919
Size: 5738
NarI GG'CG_CC
Cuts at: 3169 3417 3169
Size: 248 5490
NcoI C'CATG_G
Cuts at: 107 1224 1554 1713 107
Size: 1117 330 159 4132
Fragments arranged by size:
4132 1117 330 159
NgoMIV G'CCGG_C
Cuts at: 3917 3917
Size: 5738
NsiI A_TGCA'T
Cuts at: 291 291
Size: 5738
NspI r_CATG'y
Cuts at: 332 677 969 1096 1302 2317 2791 3291 4894
Size: 345 292 127 206 1015 474 500 1603
Cuts at: 4894 332
Size: 1176
Fragments arranged by size:
1603 1176 1015 500 474 345 292 206
127
PasI CC'CwG_GG
Cuts at: 2500 2500
Size: 5738
PciI A'CATG_T
Cuts at: 673 1298 4890 673
Size: 625 3592 1521
Fragments arranged by size:
3592 1521 625
PflMI CCAn_nnn'nTGG
Cuts at: 1230 1705 1230
Size: 475 5263
PmeI GTTT'AAAC
Cuts at: 2737 2737
Size: 5738
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 4184
Size: 32 5706
PshAI GACnn'nnGTC
Cuts at: 1973 1973
Size: 5738
PsiI TTA'TAA
Cuts at: 1562 2308 2366 1562
Size: 746 58 4934
Fragments arranged by size:
4934 746 58
PstI C_TGCA'G
Cuts at: 271 1958 2785 3470 271
Size: 1687 827 685 2539
Fragments arranged by size:
2539 1687 827 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5738
PvuII CAG'CTG
Cuts at: 266 1863 3163 3523 266
Size: 1597 1300 360 2481
Fragments arranged by size:
2481 1597 1300 360
RsrII CG'GwC_CG
Cuts at: 3933 3933
Size: 5738
SacI G_AGCT'C
Cuts at: 1332 2752 1332
Size: 1420 4318
SalI G'TCGA_C
Cuts at: 2775 2775
Size: 5738
SapI GCTCTTCn'nnn_
Cuts at: 3761 3971 3761
Size: 210 5528
SbfI CC_TGCA'GG
Cuts at: 2785 2785
Size: 5738
ScaI AGT'ACT
Cuts at: 1857 5255 1857
Size: 3398 2340
SfcI C'TryA_G
Cuts at: 33 267 1954 2781 3466 4434 4625 33
Size: 234 1687 827 685 968 191 1146
Fragments arranged by size:
1687 1146 968 827 685 234 191
SfoI GGC'GCC
Cuts at: 3170 3418 3170
Size: 248 5490
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5738
SmaI CCC'GGG
Cuts at: 2620 2760 2620
Size: 140 5598
SmlI C'TyrA_G
Cuts at: 160 2893 3808 4522 4784 160
Size: 2733 915 714 262 1114
Fragments arranged by size:
2733 1114 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5738
SphI G_CATG'C
Cuts at: 2791 3291 2791
Size: 500 5238
SspI AAT'ATT
Cuts at: 625 1650 2357 625
Size: 1025 707 4006
Fragments arranged by size:
4006 1025 707
StuI AGG'CCT
Cuts at: 227 1172 1944 227
Size: 945 772 4021
Fragments arranged by size:
4021 945 772
StyI C'CwwG_G
Cuts at: 107 507 1224 1554 1713 1945 2872 3226 107
Size: 400 717 330 159 232 927 354 2619
Fragments arranged by size:
2619 927 717 400 354 330 232 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 353 1909 2188 2492 3321 3454 3913 4122 353
Size: 1556 279 304 829 133 459 209 1969
Fragments arranged by size:
1969 1556 829 459 304 279 209 133
TatI w'GTAC_w
Cuts at: 1855 5253 1855
Size: 3398 2340
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 917 949 1489 1521 2324 2356 2412 2444 5255
Size: 32 540 32 803 32 56 32 2811
Cuts at: 5255 5287 917
Size: 32 1368
Fragments arranged by size:
2811 1368 803 540 56 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3535 3535
Size: 5738
XbaI T'CTAG_A
Cuts at: 2769 2769
Size: 5738
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2582 3233 2582
Size: 651 5087
XmaI C'CCGG_G
Cuts at: 2618 2758 2618
Size: 140 5598
XmnI GAAnn'nnTTC
Cuts at: 684 684
Size: 5738
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AlfI AloI ApaI AscI
BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI