Restriction map for
pF1KB7278
(Circular) (Six-base) MAPSORT of: pF1KB7278.seq Check: 7120 from: 1 to: 5180
pF1KB7278 5180 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 211 211
Size: 5180
AccI GT'mk_AC
Cuts at: 292 329 2218 292
Size: 37 1889 3254
Fragments arranged by size:
3254 1889 37
Acc65I G'GTAC_C
Cuts at: 2196 2196
Size: 5180
AclI AA'CG_TT
Cuts at: 594 5020 594
Size: 4426 754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2115 3005 3437 3790 4452 2115
Size: 890 432 353 662 2843
Fragments arranged by size:
2843 890 662 432 353
AfeI AGC'GCT
Cuts at: 660 660
Size: 5180
AflIII A'CryG_T
Cuts at: 2532 4332 4613 2532
Size: 1800 281 3099
Fragments arranged by size:
3099 1800 281
AgeI A'CCGG_T
Cuts at: 1395 1395
Size: 5180
AleI CACnn'nnGTG
Cuts at: 1492 1492
Size: 5180
AlwNI CAG_nnn'CTG
Cuts at: 935 1665 2485 3923 935
Size: 730 820 1438 2192
Fragments arranged by size:
2192 1438 820 730
ApaI G_GGCC'C
Cuts at: 1438 1708 1907 1438
Size: 270 199 4711
Fragments arranged by size:
4711 270 199
ApaLI G'TGCA_C
Cuts at: 1155 4018 1155
Size: 2863 2317
ApoI r'AATT_y
Cuts at: 1051 2184 4533 4789 1051
Size: 1133 2349 256 1442
Fragments arranged by size:
2349 1442 1133 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5180
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5180
AvaI C'yCGr_G
Cuts at: 774 920 1179 1234 1453 1675 2200 774
Size: 146 259 55 219 222 525 3754
Fragments arranged by size:
3754 525 259 222 219 146 55
BamHI G'GATC_C
Cuts at: 2205 2205
Size: 5180
BanI G'GyrC_C
Cuts at: 700 762 1402 1494 1543 1579 2196 2610 2858
Size: 62 640 92 49 36 617 414 248
Cuts at: 2858 2893 700
Size: 35 2987
Fragments arranged by size:
2987 640 617 414 248 92 62 49
36 35
BanII G_rGCy'C
Cuts at: 1438 1708 1828 1864 1907 2075 2194 3224 1438
Size: 270 120 36 43 168 119 1030 3394
Fragments arranged by size:
3394 1030 270 168 120 119 43 36
BbeI G_GCGC'C
Cuts at: 1406 1547 1583 2614 2862 1406
Size: 141 36 1031 248 3724
Fragments arranged by size:
3724 1031 248 141 36
BbvCI CC'TCA_GC
Cuts at: 336 955 336
Size: 619 4561
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1173 1207 1245 1279 1173
Size: 34 38 34 5074
Fragments arranged by size:
5074 38 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 108 3078 4134 5180 108
Size: 2970 1056 1046 108
Fragments arranged by size:
2970 1056 1046 108
BclI T'GATC_A
Cuts at: 2700 2700
Size: 5180
BglI GCCn_nnn'nGGC
Cuts at: 1291 1449 1291
Size: 158 5022
BglII A'GATC_T
Cuts at: 2695 4732 2695
Size: 2037 3143
BlpI GC'TnA_GC
Cuts at: 2292 2292
Size: 5180
Bme1580I G_kGCm'C
Cuts at: 703 1159 1438 1708 1907 2113 2805 2898 4022
Size: 456 279 270 199 206 692 93 1124
Cuts at: 4022 703
Size: 1861
Fragments arranged by size:
1861 1124 692 456 279 270 206 199
93
BmrI ACTGGGnnnn_n'
Cuts at: 2392 2473 2808 4952 2392
Size: 81 335 2144 2620
Fragments arranged by size:
2620 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3547 3547
Size: 5180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1738 2136 1738
Size: 398 4782
Bpu10I CC'TnA_GC
Cuts at: 244 336 955 1817 244
Size: 92 619 862 3607
Fragments arranged by size:
3607 862 619 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 2089 2356 3235 3949 4247 271
Size: 1818 267 879 714 298 1204
Fragments arranged by size:
1818 1204 879 714 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 367 4884 367
Size: 4517 663
BsaAI yAC'GTr
Cuts at: 2533 2533
Size: 5180
BsaBI GATnn'nnATC
Cuts at: 6 2694 4731 6
Size: 2688 2037 455
Fragments arranged by size:
2688 2037 455
BsaHI Gr'CG_yC
Cuts at: 1403 1544 1580 2611 2859 3561 5057 1403
Size: 141 36 1031 248 702 1496 1526
Fragments arranged by size:
1526 1496 1031 702 248 141 36
BsaWI w'CCGG_w
Cuts at: 1395 2369 2890 3568 3979 4126 4400 1395
Size: 974 521 678 411 147 274 2175
Fragments arranged by size:
2175 974 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2134 2164 2134
Size: 30 5150
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 493 1075 1321 1362 2110 493
Size: 582 246 41 748 3563
Fragments arranged by size:
3563 748 582 246 41
BseYI C'CCAG_C
Cuts at: 942 1444 1503 1653 1665 2605 3363 4028 4436
Size: 502 59 150 12 940 758 665 408
Cuts at: 4436 942
Size: 1686
Fragments arranged by size:
1686 940 758 665 502 408 150 59
12
BsiEI CG_ry'CG
Cuts at: 104 1212 1388 1898 2722 2768 3998 104
Size: 1108 176 510 824 46 1230 1286
Fragments arranged by size:
1286 1230 1108 824 510 176 46
BsiHKAI G_wGCw'C
Cuts at: 1159 1864 2194 2972 3162 4022 1159
Size: 705 330 778 190 860 2317
Fragments arranged by size:
2317 860 778 705 330 190
BsmI GAATG_Cn'
Cuts at: 225 589 225
Size: 364 4816
BsmBI CGTCTCn'nnnn_
Cuts at: 1231 1231
Size: 5180
Bsp1286I G_dGCh'C
Cuts at: 703 1159 1438 1708 1828 1864 1907 2075 2113
Size: 456 279 270 120 36 43 168 38
Cuts at: 2113 2194 2805 2898 2972 3162 3224 4022 703
Size: 81 611 93 74 190 62 798 1861
Fragments arranged by size:
1861 798 611 456 279 270 190 168
120 93 81 74 62 43 38 36
BspHI T'CATG_A
Cuts at: 3612 3612
Size: 5180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 211 2230 2444 2746 3127 211
Size: 2019 214 302 381 2264
Fragments arranged by size:
2264 2019 381 302 214
BsrBI CCG'CTC
Cuts at: 50 452 1122 1799 3472 3526 5010 5175 50
Size: 402 670 677 1673 54 1484 165 55
Fragments arranged by size:
1673 1484 677 670 402 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2004 2127 3092 2004
Size: 123 965 4092
Fragments arranged by size:
4092 965 123
BsrFI r'CCGG_y
Cuts at: 796 856 1395 1448 2087 3178 3359 796
Size: 60 539 53 639 1091 181 2617
Fragments arranged by size:
2617 1091 639 539 181 60 53
BssHII G'CGCG_C
Cuts at: 1670 1670
Size: 5180
BssSI C'ACGA_G
Cuts at: 3451 4159 3451
Size: 708 4472
BstAPI GCAn_nnn'nTGC
Cuts at: 935 2557 935
Size: 1622 3558
BstBI TT'CG_AA
Cuts at: 3541 3541
Size: 5180
BstXI CCAn_nnnn'nTGG
Cuts at: 909 1354 909
Size: 445 4735
BstYI r'GATC_y
Cuts at: 1 538 1146 2205 2672 2695 3030 3276 3582
Size: 537 608 1059 467 23 335 246 306
Cuts at: 3582 3594 3680 4732 1
Size: 12 86 1052 449
Fragments arranged by size:
1059 1052 608 537 467 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 711 1025 1382 1423 1730 1763 1880 1898
Size: 604 314 357 41 307 33 117 18
Cuts at: 1898 107
Size: 3389
Fragments arranged by size:
3389 604 357 314 307 117 41 33
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1005 2080 3130 3311 3474 1005
Size: 1075 1050 181 163 2711
Fragments arranged by size:
2711 1075 1050 181 163
BtsI GCAGTG_nn'
Cuts at: 2423 2423
Size: 5180
DraI TTT'AAA
Cuts at: 2179 2179
Size: 5180
DrdI GACnn_nn'nnGTC
Cuts at: 2886 4230 2886
Size: 1344 3836
EaeI y'GGCC_r
Cuts at: 389 899 987 1209 1292 1385 1895 2765 2939
Size: 510 88 222 83 93 510 870 174
Cuts at: 2939 3330 3357 4702 389
Size: 391 27 1345 867
Fragments arranged by size:
1345 870 867 510 510 391 222 174
93 88 83 27
EagI C'GGCC_G
Cuts at: 1209 1385 1895 2765 1209
Size: 176 510 870 3624
Fragments arranged by size:
3624 870 510 176
EarI CTCTTCn'nnn_
Cuts at: 398 1196 1222 3203 3413 398
Size: 798 26 1981 210 2165
Fragments arranged by size:
2165 1981 798 210 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1015 1084 1550 3388 4130 4276 4990 1015
Size: 69 466 1838 742 146 714 1205
Fragments arranged by size:
1838 1205 742 714 466 146 69
EcoICRI GAG'CTC
Cuts at: 1862 2192 1862
Size: 330 4850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1738 2115 2136 3005 3437 3790 4452 1738
Size: 377 21 869 432 353 662 2466
Fragments arranged by size:
2466 869 662 432 377 353 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1303 1303
Size: 5180
EcoO109I rG'GnC_Cy
Cuts at: 778 1434 1705 1787 1903 1904 2319 778
Size: 656 271 82 116 1 415 3639
Fragments arranged by size:
3639 656 415 271 116 82 1
EcoRI G'AATT_C
Cuts at: 2184 2184
Size: 5180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 5180
FspI TGC'GCA
Cuts at: 2961 2961
Size: 5180
HaeII r_GCGC'y
Cuts at: 662 1088 1262 1406 1484 1547 1583 1607 1933
Size: 426 174 144 78 63 36 24 326
Cuts at: 1933 2088 2142 2614 2862 4092 4863 662
Size: 155 54 472 248 1230 771 979
Fragments arranged by size:
1230 979 771 472 426 326 248 174
155 144 78 63 54 36 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3022 3052 3054 3084 3022
Size: 30 2 30 5118
Fragments arranged by size:
5118 30 30 2
HincII GTy'rAC
Cuts at: 2219 2219
Size: 5180
KasI G'GCGC_C
Cuts at: 1402 1543 1579 2610 2858 1402
Size: 141 36 1031 248 3724
Fragments arranged by size:
3724 1031 248 141 36
KpnI G_GTAC'C
Cuts at: 2200 2200
Size: 5180
MluI A'CGCG_T
Cuts at: 4613 4613
Size: 5180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 186 1568 2045 3939 4123 186
Size: 1382 477 1894 184 1243
Fragments arranged by size:
1894 1382 1243 477 184
MscI TGG'CCA
Cuts at: 901 2941 901
Size: 2040 3140
MslI CAynn'nnrTG
Cuts at: 1492 3169 3296 1492
Size: 1677 127 3376
Fragments arranged by size:
3376 1677 127
MspA1I CmG'CkG
Cuts at: 441 638 850 935 962 974 1384 1425 1440
Size: 197 212 85 27 12 410 41 15
Cuts at: 1440 1503 1526 1638 1647 1732 1765 1867 1873
Size: 63 23 112 9 85 33 102 6
Cuts at: 1873 1882 1900 1914 1920 2291 2605 2965 3749
Size: 9 18 14 6 371 314 360 784
Cuts at: 3749 3994 441
Size: 245 1627
Fragments arranged by size:
1627 784 410 371 360 314 245 212
197 112 102 85 85 63 41 33
27 23 18 15 14 12 9 9
6 6
NaeI GCC'GGC
Cuts at: 798 858 1450 2089 3361 798
Size: 60 592 639 1272 2617
Fragments arranged by size:
2617 1272 639 592 60
NarI GG'CG_CC
Cuts at: 1403 1544 1580 2611 2859 1403
Size: 141 36 1031 248 3724
Fragments arranged by size:
3724 1031 248 141 36
NcoI C'CATG_G
Cuts at: 107 107
Size: 5180
NdeI CA'TA_TG
Cuts at: 2036 2036
Size: 5180
NgoMIV G'CCGG_C
Cuts at: 796 856 1448 2087 3359 796
Size: 60 592 639 1272 2617
Fragments arranged by size:
2617 1272 639 592 60
NotI GC'GGCC_GC
Cuts at: 1385 1895 1385
Size: 510 4670
NspI r_CATG'y
Cuts at: 578 2233 2733 4336 578
Size: 1655 500 1603 1422
Fragments arranged by size:
1655 1603 1422 500
PciI A'CATG_T
Cuts at: 4332 4332
Size: 5180
PflMI CCAn_nnn'nTGG
Cuts at: 897 1665 897
Size: 768 4412
PfoI T'CCnGG_A
Cuts at: 1055 1055
Size: 5180
PmeI GTTT'AAAC
Cuts at: 2179 2179
Size: 5180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 5148
PpuMI rG'GwC_Cy
Cuts at: 778 1787 778
Size: 1009 4171
PspOMI G'GGCC_C
Cuts at: 1434 1704 1903 1434
Size: 270 199 4711
Fragments arranged by size:
4711 270 199
PspXI vC'TCGA_Gb
Cuts at: 1453 1675 1453
Size: 222 4958
PstI C_TGCA'G
Cuts at: 1304 1878 1919 2227 2912 1304
Size: 574 41 308 685 3572
Fragments arranged by size:
3572 685 574 308 41
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5180
PvuII CAG'CTG
Cuts at: 441 935 1503 1638 1914 1920 2605 2965 441
Size: 494 568 135 276 6 685 360 2656
Fragments arranged by size:
2656 685 568 494 360 276 135 6
RsrII CG'GwC_CG
Cuts at: 3375 3375
Size: 5180
SacI G_AGCT'C
Cuts at: 1864 2194 1864
Size: 330 4850
SacII CC_GC'GG
Cuts at: 1385 1426 1733 1766 1883 1901 1385
Size: 41 307 33 117 18 4664
Fragments arranged by size:
4664 307 117 41 33 18
SalI G'TCGA_C
Cuts at: 2217 2217
Size: 5180
SanDI GG'GwC_CC
Cuts at: 778 1787 778
Size: 1009 4171
SapI GCTCTTCn'nnn_
Cuts at: 398 3203 3413 398
Size: 2805 210 2165
Fragments arranged by size:
2805 2165 210
SbfI CC_TGCA'GG
Cuts at: 1304 2227 1304
Size: 923 4257
ScaI AGT'ACT
Cuts at: 4697 4697
Size: 5180
SexAI A'CCwGG_T
Cuts at: 2024 2024
Size: 5180
SfcI C'TryA_G
Cuts at: 33 1300 1874 1915 2223 2908 3876 4067 33
Size: 1267 574 41 308 685 968 191 1146
Fragments arranged by size:
1267 1146 968 685 574 308 191 41
SfoI GGC'GCC
Cuts at: 1404 1545 1581 2612 2860 1404
Size: 141 36 1031 248 3724
Fragments arranged by size:
3724 1031 248 141 36
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5180
SgrAI Cr'CCGG_yG
Cuts at: 856 1395 2087 856
Size: 539 692 3949
Fragments arranged by size:
3949 692 539
SmaI CCC'GGG
Cuts at: 776 922 2202 776
Size: 146 1280 3754
Fragments arranged by size:
3754 1280 146
SmlI C'TyrA_G
Cuts at: 286 1453 1675 2104 2335 3250 3964 4226 286
Size: 1167 222 429 231 915 714 262 1240
Fragments arranged by size:
1240 1167 915 714 429 262 231 222
SpeI A'CTAG_T
Cuts at: 67 486 67
Size: 419 4761
SphI G_CATG'C
Cuts at: 2233 2733 2233
Size: 500 4680
StyI C'CwwG_G
Cuts at: 107 152 704 1139 2075 2314 2668 107
Size: 45 552 435 936 239 354 2619
Fragments arranged by size:
2619 936 552 435 354 239 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2763 2896 3355 3564 2763
Size: 133 459 209 4379
Fragments arranged by size:
4379 459 209 133
TatI w'GTAC_w
Cuts at: 4695 4695
Size: 5180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 802 834 1138 1170 2134 2166 4697 4729 802
Size: 32 304 32 964 32 2531 32 1253
Fragments arranged by size:
2531 1253 964 304 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2977 2977
Size: 5180
XbaI T'CTAG_A
Cuts at: 535 2211 535
Size: 1676 3504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1146 2675 1146
Size: 1529 3651
XhoI C'TCGA_G
Cuts at: 1453 1675 1453
Size: 222 4958
XmaI C'CCGG_G
Cuts at: 774 920 2200 774
Size: 146 1280 3754
Fragments arranged by size:
3754 1280 146
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsgI BsiWI BspEI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NruI NsiI PacI
PasI PmlI PshAI PsiI PsrI SfiI SnaBI SrfI
SspI StuI SwaI XmnI ZraI