Restriction map for
pF1KB7282
(Circular) (Six-base) MAPSORT of: pF1KB7282.seq Check: 8200 from: 1 to: 5228
pF1KB7282 5228 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 2122 2266 2122
Size: 144 5084
Acc65I G'GTAC_C
Cuts at: 2244 2244
Size: 5228
AclI AA'CG_TT
Cuts at: 192 5068 192
Size: 4876 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3485 3838 4500 3053
Size: 432 353 662 3781
Fragments arranged by size:
3781 662 432 353
AflIII A'CryG_T
Cuts at: 1667 2063 2117 2580 4380 4661 1667
Size: 396 54 463 1800 281 2234
Fragments arranged by size:
2234 1800 463 396 281 54
AleI CACnn'nnGTG
Cuts at: 760 1327 1579 1753 760
Size: 567 252 174 4235
Fragments arranged by size:
4235 567 252 174
AlwNI CAG_nnn'CTG
Cuts at: 1522 2533 3971 1522
Size: 1011 1438 2779
Fragments arranged by size:
2779 1438 1011
ApaLI G'TGCA_C
Cuts at: 4066 4066
Size: 5228
ApoI r'AATT_y
Cuts at: 646 971 2145 2232 4581 4837 646
Size: 325 1174 87 2349 256 1037
Fragments arranged by size:
2349 1174 1037 325 256 87
AseI AT'TA_AT
Cuts at: 20 20
Size: 5228
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
AvaI C'yCGr_G
Cuts at: 463 470 2248 463
Size: 7 1778 3443
Fragments arranged by size:
3443 1778 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1214 1247 1214
Size: 33 5195
BamHI G'GATC_C
Cuts at: 2253 2253
Size: 5228
BanI G'GyrC_C
Cuts at: 2049 2244 2658 2906 2941 2049
Size: 195 414 248 35 4336
Fragments arranged by size:
4336 414 248 195 35
BanII G_rGCy'C
Cuts at: 751 1457 1945 2242 3272 751
Size: 706 488 297 1030 2707
Fragments arranged by size:
2707 1030 706 488 297
BbeI G_GCGC'C
Cuts at: 2662 2910 2662
Size: 248 4980
BbsI GAAGACnn'nnnn_
Cuts at: 465 1067 1124 465
Size: 602 57 4569
Fragments arranged by size:
4569 602 57
BbvCI CC'TCA_GC
Cuts at: 1639 2055 1639
Size: 416 4812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5194
BciVI GTATCCnnnnn_n'
Cuts at: 1215 1775 1807 1889 3126 4182 5228 1215
Size: 560 32 82 1237 1056 1046 1215
Fragments arranged by size:
1237 1215 1056 1046 560 82 32
BclI T'GATC_A
Cuts at: 2748 2748
Size: 5228
BfrBI ATG'CAT
Cuts at: 111 360 111
Size: 249 4979
BglII A'GATC_T
Cuts at: 663 2743 4780 663
Size: 2080 2037 1111
Fragments arranged by size:
2080 2037 1111
BlpI GC'TnA_GC
Cuts at: 2340 2340
Size: 5228
Bme1580I G_kGCm'C
Cuts at: 351 2054 2853 2946 4070 351
Size: 1703 799 93 1124 1509
Fragments arranged by size:
1703 1509 1124 799 93
BmrI ACTGGGnnnn_n'
Cuts at: 754 2020 2440 2521 2856 5000 754
Size: 1266 420 81 335 2144 982
Fragments arranged by size:
2144 1266 982 420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1766 2021 2070 3595 1766
Size: 255 49 1525 3399
Fragments arranged by size:
3399 1525 255 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 322 1086 1544 145
Size: 177 764 458 3829
Fragments arranged by size:
3829 764 458 177
Bpu10I CC'TnA_GC
Cuts at: 1639 2055 1639
Size: 416 4812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2404 3283 3997 4295 2404
Size: 879 714 298 3337
Fragments arranged by size:
3337 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1679 4932 1679
Size: 3253 1975
BsaAI yAC'GTr
Cuts at: 1407 1670 2066 2581 1407
Size: 263 396 515 4054
Fragments arranged by size:
4054 515 396 263
BsaBI GATnn'nnATC
Cuts at: 6 668 2742 4779 6
Size: 662 2074 2037 455
Fragments arranged by size:
2074 2037 662 455
BsaHI Gr'CG_yC
Cuts at: 2659 2907 3609 5105 2659
Size: 248 702 1496 2782
Fragments arranged by size:
2782 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1136 2417 2938 3616 4027 4174 4448 1136
Size: 1281 521 678 411 147 274 1916
Fragments arranged by size:
1916 1281 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1174 1204 1174
Size: 30 5198
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 706 741 1198 319
Size: 387 35 457 4349
Fragments arranged by size:
4349 457 387 35
BseYI C'CCAG_C
Cuts at: 1289 1360 1702 1710 2653 3411 4076 4484 1289
Size: 71 342 8 943 758 665 408 2033
Fragments arranged by size:
2033 943 758 665 408 342 71 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 1414 1931 1055
Size: 359 517 4352
Fragments arranged by size:
4352 517 359
BsiEI CG_ry'CG
Cuts at: 104 2770 2816 4046 104
Size: 2666 46 1230 1286
Fragments arranged by size:
2666 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1136 1412 1457 1945 2162 2242 3020 3210 4070
Size: 276 45 488 217 80 778 190 860
Cuts at: 4070 1136
Size: 2294
Fragments arranged by size:
2294 860 778 488 276 217 190 80
45
BsmI GAATG_Cn'
Cuts at: 2088 2088
Size: 5228
BsmBI CGTCTCn'nnnn_
Cuts at: 565 972 565
Size: 407 4821
Bsp1286I G_dGCh'C
Cuts at: 351 751 1136 1412 1457 1945 2054 2162 2242
Size: 400 385 276 45 488 109 108 80
Cuts at: 2242 2853 2946 3020 3210 3272 4070 351
Size: 611 93 74 190 62 798 1509
Fragments arranged by size:
1509 798 611 488 400 385 276 190
109 108 93 80 74 62 45
BspHI T'CATG_A
Cuts at: 1893 3660 1893
Size: 1767 3461
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2278 2492 2794 3175 2278
Size: 214 302 381 4331
Fragments arranged by size:
4331 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1443 3520 3574 5058 5223 50
Size: 1393 2077 54 1484 165 55
Fragments arranged by size:
2077 1484 1393 165 55 54
BsrDI GCAATG_nn'
Cuts at: 301 500 1661 3140 301
Size: 199 1161 1479 2389
Fragments arranged by size:
2389 1479 1161 199
BsrFI r'CCGG_y
Cuts at: 3226 3407 3226
Size: 181 5047
BsrGI T'GTAC_A
Cuts at: 719 719
Size: 5228
BssSI C'ACGA_G
Cuts at: 3499 4207 3499
Size: 708 4520
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 2605 1570
Size: 1035 4193
BstBI TT'CG_AA
Cuts at: 867 947 3589 867
Size: 80 2642 2506
Fragments arranged by size:
2642 2506 80
BstXI CCAn_nnnn'nTGG
Cuts at: 496 885 1581 496
Size: 389 696 4143
Fragments arranged by size:
4143 696 389
BstYI r'GATC_y
Cuts at: 1 663 2253 2720 2743 3078 3324 3630 3642
Size: 662 1590 467 23 335 246 306 12
Cuts at: 3642 3728 4780 1
Size: 86 1052 449
Fragments arranged by size:
1590 1052 662 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 2123 2123
Size: 5228
Bsu36I CC'TnA_GG
Cuts at: 689 689
Size: 5228
BtgI C'CryG_G
Cuts at: 755 1112 1226 755
Size: 357 114 4757
Fragments arranged by size:
4757 357 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1142 3178 3359 3522 1142
Size: 2036 181 163 2848
Fragments arranged by size:
2848 2036 181 163
BtsI GCAGTG_nn'
Cuts at: 351 512 634 1484 2471 351
Size: 161 122 850 987 3108
Fragments arranged by size:
3108 987 850 161 122
DraI TTT'AAA
Cuts at: 2227 2227
Size: 5228
DrdI GACnn_nn'nnGTC
Cuts at: 2934 4278 2934
Size: 1344 3884
EaeI y'GGCC_r
Cuts at: 2041 2813 2987 3378 3405 4750 2041
Size: 772 174 391 27 1345 2519
Fragments arranged by size:
2519 1345 772 391 174 27
EagI C'GGCC_G
Cuts at: 2813 2813
Size: 5228
EarI CTCTTCn'nnn_
Cuts at: 1496 1605 3251 3461 1496
Size: 109 1646 210 3263
Fragments arranged by size:
3263 1646 210 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3436 4178 4324 5038 3436
Size: 742 146 714 3626
Fragments arranged by size:
3626 742 714 146
EcoICRI GAG'CTC
Cuts at: 1455 1943 2240 1455
Size: 488 297 4443
Fragments arranged by size:
4443 488 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 322 1086 1544 3053 3485 3838 4500 145
Size: 177 764 458 1509 432 353 662 873
Fragments arranged by size:
1509 873 764 662 458 432 353 177
EcoNI CCTnn'n_nnAGG
Cuts at: 688 1740 688
Size: 1052 4176
EcoO109I rG'GnC_Cy
Cuts at: 467 1271 1706 2367 467
Size: 804 435 661 3328
Fragments arranged by size:
3328 804 661 435
EcoRI G'AATT_C
Cuts at: 2145 2232 2145
Size: 87 5141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 5228
FspI TGC'GCA
Cuts at: 3009 3009
Size: 5228
HaeII r_GCGC'y
Cuts at: 1358 2662 2910 4140 4911 1358
Size: 1304 248 1230 771 1675
Fragments arranged by size:
1675 1304 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1174 1206 1490 1522 1863 1895 3070 3100 3102
Size: 32 284 32 341 32 1175 30 2
Cuts at: 3102 3132 1174
Size: 30 3270
Fragments arranged by size:
3270 1175 341 284 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 449 1233 2267 449
Size: 784 1034 3410
Fragments arranged by size:
3410 1034 784
HpaI GTT'AAC
Cuts at: 449 449
Size: 5228
KasI G'GCGC_C
Cuts at: 2658 2906 2658
Size: 248 4980
KpnI G_GTAC'C
Cuts at: 2248 2248
Size: 5228
MluI A'CGCG_T
Cuts at: 4661 4661
Size: 5228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3987 4171 3987
Size: 184 5044
MscI TGG'CCA
Cuts at: 2043 2989 2043
Size: 946 4282
MslI CAynn'nnrTG
Cuts at: 494 505 760 1327 1579 1753 3217 3344 494
Size: 11 255 567 252 174 1464 127 2378
Fragments arranged by size:
2378 1464 567 255 252 174 127 11
MspA1I CmG'CkG
Cuts at: 1238 1714 1731 2039 2339 2653 3013 3797 4042
Size: 476 17 308 300 314 360 784 245
Cuts at: 4042 1238
Size: 2424
Fragments arranged by size:
2424 784 476 360 314 308 300 245
17
NaeI GCC'GGC
Cuts at: 3409 3409
Size: 5228
NarI GG'CG_CC
Cuts at: 2659 2907 2659
Size: 248 4980
NcoI C'CATG_G
Cuts at: 1112 1226 1112
Size: 114 5114
NgoMIV G'CCGG_C
Cuts at: 3407 3407
Size: 5228
NruI TCG'CGA
Cuts at: 1127 1127
Size: 5228
NsiI A_TGCA'T
Cuts at: 113 362 113
Size: 249 4979
NspI r_CATG'y
Cuts at: 2121 2166 2281 2781 4384 2121
Size: 45 115 500 1603 2965
Fragments arranged by size:
2965 1603 500 115 45
PciI A'CATG_T
Cuts at: 2117 4380 2117
Size: 2263 2965
PflMI CCAn_nnn'nTGG
Cuts at: 1056 1179 1056
Size: 123 5105
PfoI T'CCnGG_A
Cuts at: 1334 1975 1334
Size: 641 4587
PmeI GTTT'AAAC
Cuts at: 2227 2227
Size: 5228
PmlI CAC'GTG
Cuts at: 1670 2066 1670
Size: 396 4832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 5196
PpuMI rG'GwC_Cy
Cuts at: 467 1271 1706 467
Size: 804 435 3989
Fragments arranged by size:
3989 804 435
PshAI GACnn'nnGTC
Cuts at: 1269 1269
Size: 5228
PstI C_TGCA'G
Cuts at: 836 1491 2275 2960 836
Size: 655 784 685 3104
Fragments arranged by size:
3104 784 685 655
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5228
PvuII CAG'CTG
Cuts at: 1714 1731 2653 3013 1714
Size: 17 922 360 3929
Fragments arranged by size:
3929 922 360 17
RsrII CG'GwC_CG
Cuts at: 3423 3423
Size: 5228
SacI G_AGCT'C
Cuts at: 1457 1945 2242 1457
Size: 488 297 4443
Fragments arranged by size:
4443 488 297
SalI G'TCGA_C
Cuts at: 2265 2265
Size: 5228
SanDI GG'GwC_CC
Cuts at: 467 1706 467
Size: 1239 3989
SapI GCTCTTCn'nnn_
Cuts at: 3251 3461 3251
Size: 210 5018
SbfI CC_TGCA'GG
Cuts at: 2275 2275
Size: 5228
ScaI AGT'ACT
Cuts at: 2004 4745 2004
Size: 2741 2487
SfcI C'TryA_G
Cuts at: 33 832 1487 1646 2207 2271 2956 3924 4115
Size: 799 655 159 561 64 685 968 191
Cuts at: 4115 33
Size: 1146
Fragments arranged by size:
1146 968 799 685 655 561 191 159
64
SfoI GGC'GCC
Cuts at: 2660 2908 2660
Size: 248 4980
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
SmaI CCC'GGG
Cuts at: 2250 2250
Size: 5228
SmlI C'TyrA_G
Cuts at: 2383 3298 4012 4274 2383
Size: 915 714 262 3337
Fragments arranged by size:
3337 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5228
SphI G_CATG'C
Cuts at: 2281 2781 2281
Size: 500 4728
SspI AAT'ATT
Cuts at: 526 526
Size: 5228
StuI AGG'CCT
Cuts at: 2203 2203
Size: 5228
StyI C'CwwG_G
Cuts at: 1112 1226 2362 2716 1112
Size: 114 1136 354 3624
Fragments arranged by size:
3624 1136 354 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 552 1165 2811 2944 3403 3612 552
Size: 613 1646 133 459 209 2168
Fragments arranged by size:
2168 1646 613 459 209 133
TatI w'GTAC_w
Cuts at: 719 1628 2002 4743 719
Size: 909 374 2741 1204
Fragments arranged by size:
2741 1204 909 374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4745 4777 4745
Size: 32 5196
Tth111I GACn'n_nGTC
Cuts at: 3025 3025
Size: 5228
XbaI T'CTAG_A
Cuts at: 2259 2259
Size: 5228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1977 2723 1977
Size: 746 4482
XmaI C'CCGG_G
Cuts at: 2248 2248
Size: 5228
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BglI BmgBI BmtI BsiWI BspEI
BssHII BstEII ClaI CspCI DraIII EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI PacI PasI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI