Restriction map for
pF1KB7283
(Circular) (Six-base) MAPSORT of: pF1KB7283.seq Check: 905 from: 1 to: 5783
pF1KB7283 5783 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 525 525
Size: 5783
AatII G_ACGT'C
Cuts at: 1643 1643
Size: 5783
AccI GT'mk_AC
Cuts at: 2821 2821
Size: 5783
Acc65I G'GTAC_C
Cuts at: 1061 1394 2799 1061
Size: 333 1405 4045
Fragments arranged by size:
4045 1405 333
AclI AA'CG_TT
Cuts at: 5623 5623
Size: 5783
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 785 1585 3608 4040 4393 5055 785
Size: 800 2023 432 353 662 1513
Fragments arranged by size:
2023 1513 800 662 432 353
AflIII A'CryG_T
Cuts at: 2109 3135 4935 5216 2109
Size: 1026 1800 281 2676
Fragments arranged by size:
2676 1800 1026 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 695 2056 695
Size: 1361 4422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2377 2409 2377
Size: 32 5751
AlwNI CAG_nnn'CTG
Cuts at: 528 2707 2749 3088 4526 528
Size: 2179 42 339 1438 1785
Fragments arranged by size:
2179 1785 1438 339 42
ApaI G_GGCC'C
Cuts at: 2620 2620
Size: 5783
ApaLI G'TGCA_C
Cuts at: 4621 4621
Size: 5783
ApoI r'AATT_y
Cuts at: 625 715 1233 1804 2787 5136 5392 625
Size: 90 518 571 983 2349 256 1016
Fragments arranged by size:
2349 1016 983 571 518 256 90
AseI AT'TA_AT
Cuts at: 20 2036 20
Size: 2016 3767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5783
AvaI C'yCGr_G
Cuts at: 255 941 1769 2408 2803 255
Size: 686 828 639 395 3235
Fragments arranged by size:
3235 828 686 639 395
BamHI G'GATC_C
Cuts at: 2808 2808
Size: 5783
BanI G'GyrC_C
Cuts at: 646 1061 1394 2799 3213 3461 3496 646
Size: 415 333 1405 414 248 35 2933
Fragments arranged by size:
2933 1405 415 414 333 248 35
BanII G_rGCy'C
Cuts at: 180 216 611 2008 2620 2797 3827 180
Size: 36 395 1397 612 177 1030 2136
Fragments arranged by size:
2136 1397 1030 612 395 177 36
BbeI G_GCGC'C
Cuts at: 3217 3465 3217
Size: 248 5535
BbvCI CC'TCA_GC
Cuts at: 947 1057 2173 2425 947
Size: 110 1116 252 4305
Fragments arranged by size:
4305 1116 252 110
BciVI GTATCCnnnnn_n'
Cuts at: 763 3681 4737 5783 763
Size: 2918 1056 1046 763
Fragments arranged by size:
2918 1056 1046 763
BclI T'GATC_A
Cuts at: 2339 3303 2339
Size: 964 4819
BfrBI ATG'CAT
Cuts at: 1261 1261
Size: 5783
BglI GCCn_nnn'nGGC
Cuts at: 2030 2030
Size: 5783
BglII A'GATC_T
Cuts at: 2309 3298 5335 2309
Size: 989 2037 2757
Fragments arranged by size:
2757 2037 989
BlpI GC'TnA_GC
Cuts at: 215 2895 215
Size: 2680 3103
Bme1580I G_kGCm'C
Cuts at: 1769 2620 3408 3501 4625 1769
Size: 851 788 93 1124 2927
Fragments arranged by size:
2927 1124 851 788 93
BmrI ACTGGGnnnn_n'
Cuts at: 2995 3076 3411 5555 2995
Size: 81 335 2144 3223
Fragments arranged by size:
3223 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 390 4150 390
Size: 3760 2023
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 550 1558 2003 462
Size: 88 1008 445 4242
Fragments arranged by size:
4242 1008 445 88
Bpu10I CC'TnA_GC
Cuts at: 610 947 1057 2173 2425 610
Size: 337 110 1116 252 3968
Fragments arranged by size:
3968 1116 337 252 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 1633 2959 3838 4552 4850 150
Size: 1483 1326 879 714 298 1083
Fragments arranged by size:
1483 1326 1083 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 266 2266 2557 2760 5487 266
Size: 2000 291 203 2727 562
Fragments arranged by size:
2727 2000 562 291 203
BsaAI yAC'GTr
Cuts at: 3136 3136
Size: 5783
BsaBI GATnn'nnATC
Cuts at: 6 3297 5334 6
Size: 3291 2037 455
Fragments arranged by size:
3291 2037 455
BsaHI Gr'CG_yC
Cuts at: 1640 3214 3462 4164 5660 1640
Size: 1574 248 702 1496 1763
Fragments arranged by size:
1763 1574 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2972 3493 4171 4582 4729 5003 2972
Size: 521 678 411 147 274 3752
Fragments arranged by size:
3752 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 421 451 487 517 2556 2586 421
Size: 30 36 30 2039 30 3618
Fragments arranged by size:
3618 2039 36 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 438 445 523 2561 438
Size: 7 78 2038 3660
Fragments arranged by size:
3660 2038 78 7
BseYI C'CCAG_C
Cuts at: 150 306 1214 1575 1781 1857 1910 1968 2075
Size: 156 908 361 206 76 53 58 107
Cuts at: 2075 2346 2420 2540 3208 3966 4631 5039 150
Size: 271 74 120 668 758 665 408 894
Fragments arranged by size:
908 894 758 668 665 408 361 271
206 156 120 107 76 74 58 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 1735 995
Size: 740 5043
BsiEI CG_ry'CG
Cuts at: 104 1135 3325 3371 4601 104
Size: 1031 2190 46 1230 1286
Fragments arranged by size:
2190 1286 1230 1031 46
BsiHKAI G_wGCw'C
Cuts at: 216 611 2168 2447 2763 2797 3575 3765 4625
Size: 395 1557 279 316 34 778 190 860
Cuts at: 4625 216
Size: 1374
Fragments arranged by size:
1557 1374 860 778 395 316 279 190
34
Bsp1286I G_dGCh'C
Cuts at: 180 216 611 1769 2008 2168 2447 2620 2763
Size: 36 395 1158 239 160 279 173 143
Cuts at: 2763 2797 3408 3501 3575 3765 3827 4625 180
Size: 34 611 93 74 190 62 798 1338
Fragments arranged by size:
1338 1158 798 611 395 279 239 190
173 160 143 93 74 62 36 34
BspHI T'CATG_A
Cuts at: 1808 1892 4215 1808
Size: 84 2323 3376
Fragments arranged by size:
3376 2323 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 349 525 2833 3047 3349 3730 349
Size: 176 2308 214 302 381 2402
Fragments arranged by size:
2402 2308 381 302 214 176
BsrBI CCG'CTC
Cuts at: 50 321 387 4075 4129 5613 5778 50
Size: 271 66 3688 54 1484 165 55
Fragments arranged by size:
3688 1484 271 165 66 55 54
BsrDI GCAATG_nn'
Cuts at: 557 3695 557
Size: 3138 2645
BsrFI r'CCGG_y
Cuts at: 1064 2184 3781 3962 1064
Size: 1120 1597 181 2885
Fragments arranged by size:
2885 1597 1120 181
BssHII G'CGCG_C
Cuts at: 234 234
Size: 5783
BssSI C'ACGA_G
Cuts at: 4054 4762 4054
Size: 708 5075
BstAPI GCAn_nnn'nTGC
Cuts at: 3160 3160
Size: 5783
BstBI TT'CG_AA
Cuts at: 4144 4144
Size: 5783
BstXI CCAn_nnnn'nTGG
Cuts at: 1857 1857
Size: 5783
BstYI r'GATC_y
Cuts at: 1 160 524 2309 2808 3275 3298 3633 3879
Size: 159 364 1785 499 467 23 335 246
Cuts at: 3879 4185 4197 4283 5335 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1785 1052 499 467 449 364 335 306
246 159 86 23 12
BtgI C'CryG_G
Cuts at: 587 962 2597 2734 587
Size: 375 1635 137 3636
Fragments arranged by size:
3636 1635 375 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1512 3733 3914 4077 1512
Size: 2221 181 163 3218
Fragments arranged by size:
3218 2221 181 163
BtsI GCAGTG_nn'
Cuts at: 2650 3026 2650
Size: 376 5407
ClaI AT'CG_AT
Cuts at: 1135 1135
Size: 5783
DraI TTT'AAA
Cuts at: 2782 2782
Size: 5783
DrdI GACnn_nn'nnGTC
Cuts at: 3489 4833 3489
Size: 1344 4439
EaeI y'GGCC_r
Cuts at: 698 1139 1355 2369 2709 3368 3542 3933 3960
Size: 441 216 1014 340 659 174 391 27
Cuts at: 3960 5305 698
Size: 1345 1176
Fragments arranged by size:
1345 1176 1014 659 441 391 340 216
174 27
EagI C'GGCC_G
Cuts at: 3368 3368
Size: 5783
EarI CTCTTCn'nnn_
Cuts at: 296 1021 1600 1658 1719 2173 2455 2555 3806
Size: 725 579 58 61 454 282 100 1251
Cuts at: 3806 4016 296
Size: 210 2063
Fragments arranged by size:
2063 1251 725 579 454 282 210 100
61 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3991 4733 4879 5593 3991
Size: 742 146 714 4181
Fragments arranged by size:
4181 742 714 146
EcoICRI GAG'CTC
Cuts at: 214 609 2795 214
Size: 395 2186 3202
Fragments arranged by size:
3202 2186 395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 550 785 1558 1585 2003 3608 4040 4393
Size: 88 235 773 27 418 1605 432 353
Cuts at: 4393 5055 462
Size: 662 1190
Fragments arranged by size:
1605 1190 773 662 432 418 353 235
88 27
EcoNI CCTnn'n_nnAGG
Cuts at: 133 505 532 133
Size: 372 27 5384
Fragments arranged by size:
5384 372 27
EcoO109I rG'GnC_Cy
Cuts at: 126 194 338 371 2616 2617 2624 2922 126
Size: 68 144 33 2245 1 7 298 2987
Fragments arranged by size:
2987 2245 298 144 68 33 7 1
EcoRI G'AATT_C
Cuts at: 1233 1804 2787 1233
Size: 571 983 4229
Fragments arranged by size:
4229 983 571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 744 3802 744
Size: 3058 2725
FspI TGC'GCA
Cuts at: 3564 3564
Size: 5783
HaeII r_GCGC'y
Cuts at: 3217 3465 4695 5466 3217
Size: 248 1230 771 3534
Fragments arranged by size:
3534 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 421 453 487 519 924 956 2556 2588 3625
Size: 32 34 32 405 32 1600 32 1037
Cuts at: 3625 3655 3657 3687 421
Size: 30 2 30 2517
Fragments arranged by size:
2517 1600 1037 405 34 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 846 2822 846
Size: 1976 3807
HindIII A'AGCT_T
Cuts at: 1651 1651
Size: 5783
HpaI GTT'AAC
Cuts at: 846 846
Size: 5783
KasI G'GCGC_C
Cuts at: 3213 3461 3213
Size: 248 5535
KpnI G_GTAC'C
Cuts at: 1065 1398 2803 1065
Size: 333 1405 4045
Fragments arranged by size:
4045 1405 333
MluI A'CGCG_T
Cuts at: 2109 5216 2109
Size: 3107 2676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1702 2156 2571 4542 4726 1702
Size: 454 415 1971 184 2759
Fragments arranged by size:
2759 1971 454 415 184
MscI TGG'CCA
Cuts at: 700 1141 1357 2371 2711 3544 700
Size: 441 216 1014 340 833 2939
Fragments arranged by size:
2939 1014 833 441 340 216
MslI CAynn'nnrTG
Cuts at: 2038 2200 3772 3899 2038
Size: 162 1572 127 3922
Fragments arranged by size:
3922 1572 162 127
MspA1I CmG'CkG
Cuts at: 1214 1246 1454 1575 1792 2424 2894 3208 3568
Size: 32 208 121 217 632 470 314 360
Cuts at: 3568 4352 4597 1214
Size: 784 245 2400
Fragments arranged by size:
2400 784 632 470 360 314 245 217
208 121 32
NaeI GCC'GGC
Cuts at: 3964 3964
Size: 5783
NarI GG'CG_CC
Cuts at: 3214 3462 3214
Size: 248 5535
NcoI C'CATG_G
Cuts at: 587 962 2597 587
Size: 375 1635 3773
Fragments arranged by size:
3773 1635 375
NdeI CA'TA_TG
Cuts at: 1938 1938
Size: 5783
NgoMIV G'CCGG_C
Cuts at: 3962 3962
Size: 5783
NsiI A_TGCA'T
Cuts at: 1263 1263
Size: 5783
NspI r_CATG'y
Cuts at: 909 1833 2836 3336 4939 909
Size: 924 1003 500 1603 1753
Fragments arranged by size:
1753 1603 1003 924 500
PasI CC'CwG_GG
Cuts at: 758 2620 758
Size: 1862 3921
PciI A'CATG_T
Cuts at: 4935 4935
Size: 5783
PflMI CCAn_nnn'nTGG
Cuts at: 528 707 1052 1558 2598 2707 528
Size: 179 345 506 1040 109 3604
Fragments arranged by size:
3604 1040 506 345 179 109
PfoI T'CCnGG_A
Cuts at: 380 527 380
Size: 147 5636
PmeI GTTT'AAAC
Cuts at: 2782 2782
Size: 5783
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 4229
Size: 32 5751
PpuMI rG'GwC_Cy
Cuts at: 194 338 2624 194
Size: 144 2286 3353
Fragments arranged by size:
3353 2286 144
PspOMI G'GGCC_C
Cuts at: 2616 2616
Size: 5783
PstI C_TGCA'G
Cuts at: 1761 2830 3515 1761
Size: 1069 685 4029
Fragments arranged by size:
4029 1069 685
PvuI CG_AT'CG
Cuts at: 104 1135 104
Size: 1031 4752
PvuII CAG'CTG
Cuts at: 1214 1246 1454 1575 1792 2424 3208 3568 1214
Size: 32 208 121 217 632 784 360 3429
Fragments arranged by size:
3429 784 632 360 217 208 121 32
RsrII CG'GwC_CG
Cuts at: 3978 3978
Size: 5783
SacI G_AGCT'C
Cuts at: 216 611 2797 216
Size: 395 2186 3202
Fragments arranged by size:
3202 2186 395
SalI G'TCGA_C
Cuts at: 2820 2820
Size: 5783
SanDI GG'GwC_CC
Cuts at: 194 194
Size: 5783
SapI GCTCTTCn'nnn_
Cuts at: 296 2173 3806 4016 296
Size: 1877 1633 210 2063
Fragments arranged by size:
2063 1877 1633 210
SbfI CC_TGCA'GG
Cuts at: 2830 2830
Size: 5783
ScaI AGT'ACT
Cuts at: 1006 5300 1006
Size: 4294 1489
SexAI A'CCwGG_T
Cuts at: 116 116
Size: 5783
SfcI C'TryA_G
Cuts at: 33 1110 1757 2826 3511 4479 4670 33
Size: 1077 647 1069 685 968 191 1146
Fragments arranged by size:
1146 1077 1069 968 685 647 191
SfoI GGC'GCC
Cuts at: 3215 3463 3215
Size: 248 5535
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5783
SmaI CCC'GGG
Cuts at: 257 1771 2805 257
Size: 1514 1034 3235
Fragments arranged by size:
3235 1514 1034
SmlI C'TyrA_G
Cuts at: 165 1648 2938 3853 4567 4829 165
Size: 1483 1290 915 714 262 1119
Fragments arranged by size:
1483 1290 1119 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5783
SphI G_CATG'C
Cuts at: 909 2836 3336 909
Size: 1927 500 3356
Fragments arranged by size:
3356 1927 500
SspI AAT'ATT
Cuts at: 869 869
Size: 5783
StuI AGG'CCT
Cuts at: 2024 2336 2024
Size: 312 5471
StyI C'CwwG_G
Cuts at: 470 587 650 962 1152 1986 2555 2597 2917
Size: 117 63 312 190 834 569 42 320
Cuts at: 2917 3271 470
Size: 354 2982
Fragments arranged by size:
2982 834 569 354 320 312 190 117
63 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1565 2554 3366 3499 3958 4167 1565
Size: 989 812 133 459 209 3181
Fragments arranged by size:
3181 989 812 459 209 133
TatI w'GTAC_w
Cuts at: 1004 2063 5298 1004
Size: 1059 3235 1489
Fragments arranged by size:
3235 1489 1059
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1716 1748 5300 5332 1716
Size: 32 3552 32 2167
Fragments arranged by size:
3552 2167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1821 3580 1821
Size: 1759 4024
XbaI T'CTAG_A
Cuts at: 2814 2814
Size: 5783
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1533 1555 3278 1533
Size: 22 1723 4038
Fragments arranged by size:
4038 1723 22
XmaI C'CCGG_G
Cuts at: 255 1769 2803 255
Size: 1514 1034 3235
Fragments arranged by size:
3235 1514 1034
ZraI GAC'GTC
Cuts at: 1641 1641
Size: 5783
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AscI AvrII BaeI
BbsI BcgI BmgBI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI MfeI NheI NotI NruI PacI PmlI PshAI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI