Restriction map for
pF1KB7287
(Circular) (Six-base) MAPSORT of: pF1KB7287.seq Check: 3862 from: 1 to: 6866
pF1KB7287 6866 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 424 1307 424
Size: 883 5983
AccI GT'mk_AC
Cuts at: 2735 3904 2735
Size: 1169 5697
Acc65I G'GTAC_C
Cuts at: 3882 3882
Size: 6866
AclI AA'CG_TT
Cuts at: 6706 6706
Size: 6866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1922 1985 2698 3547 3854 4691 5123 5476 6138
Size: 63 713 849 307 837 432 353 662
Cuts at: 6138 1922
Size: 2650
Fragments arranged by size:
2650 849 837 713 662 432 353 307
63
AfeI AGC'GCT
Cuts at: 253 622 253
Size: 369 6497
AflIII A'CryG_T
Cuts at: 824 4218 6018 6299 824
Size: 3394 1800 281 1391
Fragments arranged by size:
3394 1800 1391 281
AleI CACnn'nnGTG
Cuts at: 856 1550 2626 856
Size: 694 1076 5096
Fragments arranged by size:
5096 1076 694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1024 1024
Size: 6866
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2224 2256 2224
Size: 32 6834
AlwNI CAG_nnn'CTG
Cuts at: 213 416 879 1410 2862 3820 4171 5609 213
Size: 203 463 531 1452 958 351 1438 1470
Fragments arranged by size:
1470 1452 1438 958 531 463 351 203
ApaI G_GGCC'C
Cuts at: 394 1143 394
Size: 749 6117
ApaLI G'TGCA_C
Cuts at: 5704 5704
Size: 6866
ApoI r'AATT_y
Cuts at: 1831 2040 3870 6219 6475 1831
Size: 209 1830 2349 256 2222
Fragments arranged by size:
2349 2222 1830 256 209
AseI AT'TA_AT
Cuts at: 20 2441 2492 20
Size: 2421 51 4394
Fragments arranged by size:
4394 2421 51
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6866
AvaI C'yCGr_G
Cuts at: 300 393 596 3886 300
Size: 93 203 3290 3280
Fragments arranged by size:
3290 3280 203 93
BamHI G'GATC_C
Cuts at: 1969 3494 3891 1969
Size: 1525 397 4944
Fragments arranged by size:
4944 1525 397
BanI G'GyrC_C
Cuts at: 266 421 1000 2503 3838 3882 4296 4544 4579
Size: 155 579 1503 1335 44 414 248 35
Cuts at: 4579 266
Size: 2553
Fragments arranged by size:
2553 1503 1335 579 414 248 155 44
35
BanII G_rGCy'C
Cuts at: 116 394 905 1143 1433 1529 1930 1981 3828
Size: 278 511 238 290 96 401 51 1847
Cuts at: 3828 3880 4910 116
Size: 52 1030 2072
Fragments arranged by size:
2072 1847 1030 511 401 290 278 238
96 52 51
BbeI G_GCGC'C
Cuts at: 4300 4548 4300
Size: 248 6618
BbsI GAAGACnn'nnnn_
Cuts at: 423 2610 3185 3275 3666 423
Size: 2187 575 90 391 3623
Fragments arranged by size:
3623 2187 575 391 90
BbvCI CC'TCA_GC
Cuts at: 2574 2935 2574
Size: 361 6505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1417 1451 1417
Size: 34 6832
BciVI GTATCCnnnnn_n'
Cuts at: 4764 5820 6866 4764
Size: 1056 1046 4764
Fragments arranged by size:
4764 1056 1046
BclI T'GATC_A
Cuts at: 1265 1574 2696 4386 1265
Size: 309 1122 1690 3745
Fragments arranged by size:
3745 1690 1122 309
BglI GCCn_nnn'nGGC
Cuts at: 137 210 988 137
Size: 73 778 6015
Fragments arranged by size:
6015 778 73
BglII A'GATC_T
Cuts at: 3563 4381 6418 3563
Size: 818 2037 4011
Fragments arranged by size:
4011 2037 818
BlpI GC'TnA_GC
Cuts at: 3978 3978
Size: 6866
Bme1580I G_kGCm'C
Cuts at: 269 394 513 1003 1143 4491 4584 5708 269
Size: 125 119 490 140 3348 93 1124 1427
Fragments arranged by size:
3348 1427 1124 490 140 125 119 93
BmgBI CAC'GTC
Cuts at: 675 675
Size: 6866
BmrI ACTGGGnnnn_n'
Cuts at: 4078 4159 4494 6638 4078
Size: 81 335 2144 4306
Fragments arranged by size:
4306 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5233 5233
Size: 6866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 1216 1754 1995 2184 2227 2884 3842 1180
Size: 36 538 241 189 43 657 958 4204
Fragments arranged by size:
4204 958 657 538 241 189 43 36
Bpu10I CC'TnA_GC
Cuts at: 1403 1523 2574 2935 1403
Size: 120 1051 361 5334
Fragments arranged by size:
5334 1051 361 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 729 4042 4921 5635 5933 724
Size: 5 3313 879 714 298 1657
Fragments arranged by size:
3313 1657 879 714 298 5
BsaI GGTCTCn'nnnn_
Cuts at: 1570 2440 2722 3505 6570 1570
Size: 870 282 783 3065 1866
Fragments arranged by size:
3065 1866 870 783 282
BsaAI yAC'GTr
Cuts at: 827 927 4219 827
Size: 100 3292 3474
Fragments arranged by size:
3474 3292 100
BsaBI GATnn'nnATC
Cuts at: 6 1324 1633 2707 3376 4380 6417 6
Size: 1318 309 1074 669 1004 2037 455
Fragments arranged by size:
2037 1318 1074 1004 669 455 309
BsaHI Gr'CG_yC
Cuts at: 169 4297 4545 5247 6743 169
Size: 4128 248 702 1496 292
Fragments arranged by size:
4128 1496 702 292 248
BsaWI w'CCGG_w
Cuts at: 1937 2420 2960 3368 4055 4576 5254 5665 5812
Size: 483 540 408 687 521 678 411 147
Cuts at: 5812 6086 1937
Size: 274 2717
Fragments arranged by size:
2717 687 678 540 521 483 411 408
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 441 471 802 832 1932 1962 2200 2230 441
Size: 30 331 30 1100 30 238 30 5077
Fragments arranged by size:
5077 1100 331 238 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1920 2926 3034 3523 1920
Size: 1006 108 489 5263
Fragments arranged by size:
5263 1006 489 108
BseYI C'CCAG_C
Cuts at: 387 753 1142 2974 3805 4291 5049 5714 6122
Size: 366 389 1832 831 486 758 665 408
Cuts at: 6122 387
Size: 1131
Fragments arranged by size:
1832 1131 831 758 665 486 408 389
366
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 2344 2669 481
Size: 1863 325 4678
Fragments arranged by size:
4678 1863 325
BsiEI CG_ry'CG
Cuts at: 104 179 205 4408 4454 5684 104
Size: 75 26 4203 46 1230 1286
Fragments arranged by size:
4203 1286 1230 75 46 26
BsiHKAI G_wGCw'C
Cuts at: 116 932 1241 1298 1433 1930 2213 3294 3880
Size: 816 309 57 135 497 283 1081 586
Cuts at: 3880 4658 4848 5708 116
Size: 778 190 860 1274
Fragments arranged by size:
1274 1081 860 816 778 586 497 309
283 190 135 57
BsmI GAATG_Cn'
Cuts at: 3130 3345 3130
Size: 215 6651
Bsp1286I G_dGCh'C
Cuts at: 116 269 394 513 905 932 1003 1143 1241
Size: 153 125 119 392 27 71 140 98
Cuts at: 1241 1298 1433 1529 1930 1981 2213 3294 3828
Size: 57 135 96 401 51 232 1081 534
Cuts at: 3828 3880 4491 4584 4658 4848 4910 5708 116
Size: 52 611 93 74 190 62 798 1274
Fragments arranged by size:
1274 1081 798 611 534 401 392 232
190 153 140 135 125 119 98 96
93 74 71 62 57 52 51 27
BspEI T'CCGG_A
Cuts at: 2420 2960 3368 2420
Size: 540 408 5918
Fragments arranged by size:
5918 540 408
BspHI T'CATG_A
Cuts at: 2699 5298 2699
Size: 2599 4267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 424 839 1005 1307 3916 4130 4432 4813 424
Size: 415 166 302 2609 214 302 381 2477
Fragments arranged by size:
2609 2477 415 381 302 302 214 166
BsrBI CCG'CTC
Cuts at: 50 452 738 972 1221 5158 5212 6696 6861
Size: 402 286 234 249 3937 54 1484 165
Cuts at: 6861 50
Size: 55
Fragments arranged by size:
3937 1484 402 286 249 234 165 55
54
BsrDI GCAATG_nn'
Cuts at: 3149 3268 4778 3149
Size: 119 1510 5237
Fragments arranged by size:
5237 1510 119
BsrFI r'CCGG_y
Cuts at: 178 766 991 3785 4864 5045 178
Size: 588 225 2794 1079 181 1999
Fragments arranged by size:
2794 1999 1079 588 225 181
BssSI C'ACGA_G
Cuts at: 1225 5137 5845 1225
Size: 3912 708 2246
Fragments arranged by size:
3912 2246 708
BstAPI GCAn_nnn'nTGC
Cuts at: 213 4243 213
Size: 4030 2836
BstBI TT'CG_AA
Cuts at: 5227 5227
Size: 6866
BstEII G'GTnAC_C
Cuts at: 849 2640 849
Size: 1791 5075
BstXI CCAn_nnnn'nTGG
Cuts at: 2982 2982
Size: 6866
BstYI r'GATC_y
Cuts at: 1 875 1319 1969 3494 3563 3891 4358 4381
Size: 874 444 650 1525 69 328 467 23
Cuts at: 4381 4716 4962 5268 5280 5366 6418 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1525 1052 874 650 467 449 444 335
328 306 246 86 69 23 12
Bsu36I CC'TnA_GG
Cuts at: 1837 1877 2099 1837
Size: 40 222 6604
Fragments arranged by size:
6604 222 40
BtgI C'CryG_G
Cuts at: 107 728 1812 2636 2850 3384 3686 107
Size: 621 1084 824 214 534 302 3287
Fragments arranged by size:
3287 1084 824 621 534 302 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1315 4816 4997 5160 1315
Size: 3501 181 163 3021
Fragments arranged by size:
3501 3021 181 163
BtsI GCAGTG_nn'
Cuts at: 462 1088 1467 1545 2693 2809 4109 462
Size: 626 379 78 1148 116 1300 3219
Fragments arranged by size:
3219 1300 1148 626 379 116 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2757 2792 2757
Size: 35 6831
DraI TTT'AAA
Cuts at: 2193 3865 2193
Size: 1672 5194
DrdI GACnn_nn'nnGTC
Cuts at: 3798 4572 5916 3798
Size: 774 1344 4748
Fragments arranged by size:
4748 1344 774
EaeI y'GGCC_r
Cuts at: 176 202 860 989 2633 2822 3230 3489 3683
Size: 26 658 129 1644 189 408 259 194
Cuts at: 3683 4451 4625 5016 5043 6388 176
Size: 768 174 391 27 1345 654
Fragments arranged by size:
1644 1345 768 658 654 408 391 259
194 189 174 129 27 26
EagI C'GGCC_G
Cuts at: 176 202 4451 176
Size: 26 4249 2591
Fragments arranged by size:
4249 2591 26
EarI CTCTTCn'nnn_
Cuts at: 937 946 1799 2154 3618 4889 5099 937
Size: 9 853 355 1464 1271 210 2704
Fragments arranged by size:
2704 1464 1271 853 355 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3804 5074 5816 5962 6676 3804
Size: 1270 742 146 714 3994
Fragments arranged by size:
3994 1270 742 714 146
EcoICRI GAG'CTC
Cuts at: 114 1431 1928 3878 114
Size: 1317 497 1950 3102
Fragments arranged by size:
3102 1950 1317 497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 1216 1754 1922 1985 1995 2184 2227 2698
Size: 36 538 168 63 10 189 43 471
Cuts at: 2698 2884 3547 3842 3854 4691 5123 5476 6138
Size: 186 663 295 12 837 432 353 662
Cuts at: 6138 1180
Size: 1908
Fragments arranged by size:
1908 837 663 662 538 471 432 353
295 189 186 168 63 43 36 12
10
EcoNI CCTnn'n_nnAGG
Cuts at: 3001 3001
Size: 6866
EcoO109I rG'GnC_Cy
Cuts at: 4005 4005
Size: 6866
EcoRI G'AATT_C
Cuts at: 1831 3870 1831
Size: 2039 4827
EcoRV GAT'ATC
Cuts at: 1908 2592 1908
Size: 684 6182
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2268 4885 2268
Size: 2617 4249
FseI GG_CCGG'CC
Cuts at: 995 995
Size: 6866
FspI TGC'GCA
Cuts at: 580 4647 580
Size: 4067 2799
HaeII r_GCGC'y
Cuts at: 197 255 373 624 1149 3416 4300 4548 5778
Size: 58 118 251 525 2267 884 248 1230
Cuts at: 5778 6549 197
Size: 771 514
Fragments arranged by size:
2267 1230 884 771 525 514 251 248
118 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 802 834 1681 1713 2153 2185 2581 2613 3076
Size: 32 847 32 440 32 396 32 463
Cuts at: 3076 3108 3548 3580 4708 4738 4740 4770 802
Size: 32 440 32 1128 30 2 30 2898
Fragments arranged by size:
2898 1128 847 463 440 440 396 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1620 3905 1620
Size: 2285 4581
HindIII A'AGCT_T
Cuts at: 1151 2075 1151
Size: 924 5942
KasI G'GCGC_C
Cuts at: 4296 4544 4296
Size: 248 6618
KpnI G_GTAC'C
Cuts at: 3886 3886
Size: 6866
MluI A'CGCG_T
Cuts at: 6299 6299
Size: 6866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2416 3654 5625 5809 2416
Size: 1238 1971 184 3473
Fragments arranged by size:
3473 1971 1238 184
MscI TGG'CCA
Cuts at: 862 2635 2824 4627 862
Size: 1773 189 1803 3101
Fragments arranged by size:
3101 1803 1773 189
MslI CAynn'nnrTG
Cuts at: 856 1096 1334 1550 2626 3226 3232 3287 4855
Size: 240 238 216 1076 600 6 55 1568
Cuts at: 4855 4982 856
Size: 127 2740
Fragments arranged by size:
2740 1568 1076 600 240 238 216 127
55 6
MspA1I CmG'CkG
Cuts at: 210 281 287 354 446 464 470 842 966
Size: 71 6 67 92 18 6 372 124
Cuts at: 966 1108 2578 2862 3688 3809 3977 4291 4651
Size: 142 1470 284 826 121 168 314 360
Cuts at: 4651 5435 5680 210
Size: 784 245 1396
Fragments arranged by size:
1470 1396 826 784 372 360 314 284
245 168 142 124 121 92 71 67
18 6 6
NaeI GCC'GGC
Cuts at: 180 993 5047 180
Size: 813 4054 1999
Fragments arranged by size:
4054 1999 813
NarI GG'CG_CC
Cuts at: 4297 4545 4297
Size: 248 6618
NcoI C'CATG_G
Cuts at: 107 728 1812 2636 2850 107
Size: 621 1084 824 214 4123
Fragments arranged by size:
4123 1084 824 621 214
NgoMIV G'CCGG_C
Cuts at: 178 991 5045 178
Size: 813 4054 1999
Fragments arranged by size:
4054 1999 813
NspI r_CATG'y
Cuts at: 2086 2958 3919 4419 6022 2086
Size: 872 961 500 1603 2930
Fragments arranged by size:
2930 1603 961 872 500
PacI TTA_AT'TAA
Cuts at: 1868 1868
Size: 6866
PasI CC'CwG_GG
Cuts at: 1744 1744
Size: 6866
PciI A'CATG_T
Cuts at: 6018 6018
Size: 6866
PflMI CCAn_nnn'nTGG
Cuts at: 879 1071 3120 879
Size: 192 2049 4625
Fragments arranged by size:
4625 2049 192
PfoI T'CCnGG_A
Cuts at: 434 1157 1256 1972 434
Size: 723 99 716 5328
Fragments arranged by size:
5328 723 716 99
PmeI GTTT'AAAC
Cuts at: 3865 3865
Size: 6866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5312 5344 5312
Size: 32 6834
PsiI TTA'TAA
Cuts at: 2399 2399
Size: 6866
PspOMI G'GGCC_C
Cuts at: 390 1139 390
Size: 749 6117
PstI C_TGCA'G
Cuts at: 836 1016 1304 3913 4598 836
Size: 180 288 2609 685 3104
Fragments arranged by size:
3104 2609 685 288 180
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6866
PvuII CAG'CTG
Cuts at: 354 1108 2578 2862 4291 4651 354
Size: 754 1470 284 1429 360 2569
Fragments arranged by size:
2569 1470 1429 754 360 284
RsrII CG'GwC_CG
Cuts at: 5061 5061
Size: 6866
SacI G_AGCT'C
Cuts at: 116 1433 1930 3880 116
Size: 1317 497 1950 3102
Fragments arranged by size:
3102 1950 1317 497
SacII CC_GC'GG
Cuts at: 3689 3689
Size: 6866
SalI G'TCGA_C
Cuts at: 3903 3903
Size: 6866
SapI GCTCTTCn'nnn_
Cuts at: 937 946 4889 5099 937
Size: 9 3943 210 2704
Fragments arranged by size:
3943 2704 210 9
SbfI CC_TGCA'GG
Cuts at: 3913 3913
Size: 6866
ScaI AGT'ACT
Cuts at: 2611 3676 6383 2611
Size: 1065 2707 3094
Fragments arranged by size:
3094 2707 1065
SexAI A'CCwGG_T
Cuts at: 1064 1064
Size: 6866
SfcI C'TryA_G
Cuts at: 33 546 832 951 1012 1300 3909 4594 5562
Size: 513 286 119 61 288 2609 685 968
Cuts at: 5562 5753 33
Size: 191 1146
Fragments arranged by size:
2609 1146 968 685 513 288 286 191
119 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 988 988
Size: 6866
SfoI GGC'GCC
Cuts at: 4298 4546 4298
Size: 248 6618
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6866
SmaI CCC'GGG
Cuts at: 395 3888 395
Size: 3493 3373
SmlI C'TyrA_G
Cuts at: 708 739 4021 4936 5650 5912 708
Size: 31 3282 915 714 262 1662
Fragments arranged by size:
3282 1662 915 714 262 31
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6866
SphI G_CATG'C
Cuts at: 3919 4419 3919
Size: 500 6366
StuI AGG'CCT
Cuts at: 890 2744 3242 890
Size: 1854 498 4514
Fragments arranged by size:
4514 1854 498
StyI C'CwwG_G
Cuts at: 107 728 1812 2636 2798 2850 4000 4354 107
Size: 621 1084 824 162 52 1150 354 2619
Fragments arranged by size:
2619 1150 1084 824 621 354 162 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 846 886 2491 3023 3197 3819 4449 4582 5041
Size: 40 1605 532 174 622 630 133 459
Cuts at: 5041 5250 846
Size: 209 2462
Fragments arranged by size:
2462 1605 630 622 532 459 209 174
133 40
TatI w'GTAC_w
Cuts at: 836 2609 2867 2898 3674 6381 836
Size: 1773 258 31 776 2707 1321
Fragments arranged by size:
2707 1773 1321 776 258 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 441 473 1932 1964 6383 6415 441
Size: 32 1459 32 4419 32 892
Fragments arranged by size:
4419 1459 892 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1395 4663 1395
Size: 3268 3598
XbaI T'CTAG_A
Cuts at: 3897 3897
Size: 6866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 876 1191 1770 2832 4361 876
Size: 315 579 1062 1529 3381
Fragments arranged by size:
3381 1529 1062 579 315
XmaI C'CCGG_G
Cuts at: 393 3886 393
Size: 3493 3373
XmnI GAAnn'nnTTC
Cuts at: 1831 1831
Size: 6866
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI DraIII
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PmlI PpuMI PshAI PspXI PsrI SanDI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI