Restriction map for
pF1KB7289
(Circular) (Six-base) MAPSORT of: pF1KB7289.seq Check: 1363 from: 1 to: 6077
pF1KB7289 6077 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 3115 3115
Size: 6077
Acc65I G'GTAC_C
Cuts at: 3093 3093
Size: 6077
AclI AA'CG_TT
Cuts at: 1472 5917 1472
Size: 4445 1632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 612 736 1162 2525 3902 4334 4687 5349
Size: 92 124 426 1363 1377 432 353 662
Cuts at: 5349 520
Size: 1248
Fragments arranged by size:
1377 1363 1248 662 432 426 353 124
92
AfeI AGC'GCT
Cuts at: 1700 1700
Size: 6077
AflIII A'CryG_T
Cuts at: 2495 3429 5229 5510 2495
Size: 934 1800 281 3062
Fragments arranged by size:
3062 1800 934 281
AleI CACnn'nnGTG
Cuts at: 1418 1735 1418
Size: 317 5760
AlwNI CAG_nnn'CTG
Cuts at: 591 830 1824 2386 2418 3382 4820 591
Size: 239 994 562 32 964 1438 1848
Fragments arranged by size:
1848 1438 994 964 562 239 32
ApaI G_GGCC'C
Cuts at: 808 3025 808
Size: 2217 3860
ApaLI G'TGCA_C
Cuts at: 4915 4915
Size: 6077
ApoI r'AATT_y
Cuts at: 850 3081 5430 5686 850
Size: 2231 2349 256 1241
Fragments arranged by size:
2349 2231 1241 256
AscI GG'CGCG_CC
Cuts at: 2204 2204
Size: 6077
AseI AT'TA_AT
Cuts at: 20 20
Size: 6077
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6077
AvaI C'yCGr_G
Cuts at: 1463 2221 3097 1463
Size: 758 876 4443
Fragments arranged by size:
4443 876 758
BamHI G'GATC_C
Cuts at: 3102 3102
Size: 6077
BanI G'GyrC_C
Cuts at: 1708 2802 3093 3507 3755 3790 1708
Size: 1094 291 414 248 35 3995
Fragments arranged by size:
3995 1094 414 291 248 35
BanII G_rGCy'C
Cuts at: 808 1187 2059 2198 2339 2445 2921 3025 3091
Size: 379 872 139 141 106 476 104 66
Cuts at: 3091 4121 808
Size: 1030 2764
Fragments arranged by size:
2764 1030 872 476 379 141 139 106
104 66
BbeI G_GCGC'C
Cuts at: 3511 3759 3511
Size: 248 5829
BbsI GAAGACnn'nnnn_
Cuts at: 355 461 697 2264 355
Size: 106 236 1567 4168
Fragments arranged by size:
4168 1567 236 106
BbvCI CC'TCA_GC
Cuts at: 844 1252 844
Size: 408 5669
BciVI GTATCCnnnnn_n'
Cuts at: 424 1295 3975 5031 6077 424
Size: 871 2680 1056 1046 424
Fragments arranged by size:
2680 1056 1046 871 424
BclI T'GATC_A
Cuts at: 3597 3597
Size: 6077
BglI GCCn_nnn'nGGC
Cuts at: 745 2343 745
Size: 1598 4479
BglII A'GATC_T
Cuts at: 3592 5629 3592
Size: 2037 4040
BlpI GC'TnA_GC
Cuts at: 1268 1900 2536 3189 1268
Size: 632 636 653 4156
Fragments arranged by size:
4156 653 636 632
Bme1580I G_kGCm'C
Cuts at: 808 2726 2805 3025 3702 3795 4919 808
Size: 1918 79 220 677 93 1124 1966
Fragments arranged by size:
1966 1918 1124 677 220 93 79
BmrI ACTGGGnnnn_n'
Cuts at: 162 3289 3370 3705 5849 162
Size: 3127 81 335 2144 390
Fragments arranged by size:
3127 2144 390 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1002 4444 1002
Size: 3442 2635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 342 525 1035 1373 1978 2195 2716 2989
Size: 125 183 510 338 605 217 521 273
Cuts at: 2989 217
Size: 3305
Fragments arranged by size:
3305 605 521 510 338 273 217 183
125
Bpu10I CC'TnA_GC
Cuts at: 844 1252 2108 2677 844
Size: 408 856 569 4244
Fragments arranged by size:
4244 856 569 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3253 4132 4846 5144 3253
Size: 879 714 298 4186
Fragments arranged by size:
4186 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2884 5781 2884
Size: 2897 3180
BsaAI yAC'GTr
Cuts at: 1939 2658 3430 1939
Size: 719 772 4586
Fragments arranged by size:
4586 772 719
BsaBI GATnn'nnATC
Cuts at: 6 386 1519 3591 5628 6
Size: 380 1133 2072 2037 455
Fragments arranged by size:
2072 2037 1133 455 380
BsaHI Gr'CG_yC
Cuts at: 2684 3508 3756 4458 5954 2684
Size: 824 248 702 1496 2807
Fragments arranged by size:
2807 1496 824 702 248
BsaWI w'CCGG_w
Cuts at: 245 2768 3266 3787 4465 4876 5023 5297 245
Size: 2523 498 521 678 411 147 274 1025
Fragments arranged by size:
2523 1025 678 521 498 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1711 1741 1711
Size: 30 6047
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 974 2713 2977 3013 3061 974
Size: 1739 264 36 48 3990
Fragments arranged by size:
3990 1739 264 48 36
BseYI C'CCAG_C
Cuts at: 571 2052 2185 2433 3025 3502 4260 4925 5333
Size: 1481 133 248 592 477 758 665 408
Cuts at: 5333 571
Size: 1315
Fragments arranged by size:
1481 1315 758 665 592 477 408 248
133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 2593 2761 180
Size: 2413 168 3496
Fragments arranged by size:
3496 2413 168
BsiEI CG_ry'CG
Cuts at: 104 2071 3619 3665 4895 104
Size: 1967 1548 46 1230 1286
Fragments arranged by size:
1967 1548 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2445 3091 3869 4059 4919 2445
Size: 646 778 190 860 3603
Fragments arranged by size:
3603 860 778 646 190
Bsp1286I G_dGCh'C
Cuts at: 808 1187 2059 2198 2339 2445 2726 2805 2921
Size: 379 872 139 141 106 281 79 116
Cuts at: 2921 3025 3091 3702 3795 3869 4059 4121 4919
Size: 104 66 611 93 74 190 62 798
Cuts at: 4919 808
Size: 1966
Fragments arranged by size:
1966 872 798 611 379 281 190 141
139 116 106 104 93 79 74 66
62
BspEI T'CCGG_A
Cuts at: 245 2768 245
Size: 2523 3554
BspHI T'CATG_A
Cuts at: 1502 4509 1502
Size: 3007 3070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2380 2840 3127 3341 3643 4024 2380
Size: 460 287 214 302 381 4433
Fragments arranged by size:
4433 460 381 302 287 214
BsrBI CCG'CTC
Cuts at: 50 2067 4369 4423 5907 6072 50
Size: 2017 2302 54 1484 165 55
Fragments arranged by size:
2302 2017 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 818 1307 3989 818
Size: 489 2682 2906
Fragments arranged by size:
2906 2682 489
BsrFI r'CCGG_y
Cuts at: 2216 4075 4256 2216
Size: 1859 181 4037
Fragments arranged by size:
4037 1859 181
BssHII G'CGCG_C
Cuts at: 2121 2204 2121
Size: 83 5994
BssSI C'ACGA_G
Cuts at: 4348 5056 4348
Size: 708 5369
BstAPI GCAn_nnn'nTGC
Cuts at: 1278 3454 1278
Size: 2176 3901
BstBI TT'CG_AA
Cuts at: 1748 4438 1748
Size: 2690 3387
BstEII G'GTnAC_C
Cuts at: 600 981 600
Size: 381 5696
BstXI CCAn_nnnn'nTGG
Cuts at: 667 1788 1812 667
Size: 1121 24 4932
Fragments arranged by size:
4932 1121 24
BstYI r'GATC_y
Cuts at: 1 461 1661 3102 3569 3592 3927 4173 4479
Size: 460 1200 1441 467 23 335 246 306
Cuts at: 4479 4491 4577 5629 1
Size: 12 86 1052 449
Fragments arranged by size:
1441 1200 1052 467 460 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2837 2837
Size: 6077
BtgI C'CryG_G
Cuts at: 107 368 791 107
Size: 261 423 5393
Fragments arranged by size:
5393 423 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1198 4027 4208 4371 1198
Size: 2829 181 163 2904
Fragments arranged by size:
2904 2829 181 163
BtsI GCAGTG_nn'
Cuts at: 192 786 1073 1094 2424 3320 192
Size: 594 287 21 1330 896 2949
Fragments arranged by size:
2949 1330 896 594 287 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 133 168 978 1013 133
Size: 35 810 35 5197
Fragments arranged by size:
5197 810 35 35
DraI TTT'AAA
Cuts at: 3076 3076
Size: 6077
DrdI GACnn_nn'nnGTC
Cuts at: 3783 5127 3783
Size: 1344 4733
EaeI y'GGCC_r
Cuts at: 294 746 1526 1613 1802 2355 3005 3662 3836
Size: 452 780 87 189 553 650 657 174
Cuts at: 3836 4227 4254 5599 294
Size: 391 27 1345 772
Fragments arranged by size:
1345 780 772 657 650 553 452 391
189 174 87 27
EagI C'GGCC_G
Cuts at: 3662 3662
Size: 6077
EarI CTCTTCn'nnn_
Cuts at: 631 643 1498 2327 2501 2990 4100 4310 631
Size: 12 855 829 174 489 1110 210 2398
Fragments arranged by size:
2398 1110 855 829 489 210 174 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2737 4285 5027 5173 5887 2737
Size: 1548 742 146 714 2927
Fragments arranged by size:
2927 1548 742 714 146
EcoICRI GAG'CTC
Cuts at: 2443 3089 2443
Size: 646 5431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 342 520 525 612 736 1035 1162 1373
Size: 125 178 5 87 124 299 127 211
Cuts at: 1373 1978 2195 2525 2716 2989 3902 4334 4687
Size: 605 217 330 191 273 913 432 353
Cuts at: 4687 5349 217
Size: 662 945
Fragments arranged by size:
945 913 662 605 432 353 330 299
273 217 211 191 178 127 125 124
87 5
EcoNI CCTnn'n_nnAGG
Cuts at: 427 2836 427
Size: 2409 3668
EcoO109I rG'GnC_Cy
Cuts at: 228 259 804 805 1248 2082 3021 3022 3216
Size: 31 545 1 443 834 939 1 194
Cuts at: 3216 228
Size: 3089
Fragments arranged by size:
3089 939 834 545 443 194 31 1
1
EcoRI G'AATT_C
Cuts at: 3081 3081
Size: 6077
EcoRV GAT'ATC
Cuts at: 903 903
Size: 6077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4096 4096
Size: 6077
FseI GG_CCGG'CC
Cuts at: 2220 2220
Size: 6077
FspI TGC'GCA
Cuts at: 3858 3858
Size: 6077
HaeII r_GCGC'y
Cuts at: 635 1046 1702 2097 3511 3759 4989 5760 635
Size: 411 656 395 1414 248 1230 771 952
Fragments arranged by size:
1414 1230 952 771 656 411 395 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2390 2422 2482 2514 2769 2801 3919 3949 3951
Size: 32 60 32 255 32 1118 30 2
Cuts at: 3951 3981 2390
Size: 30 4486
Fragments arranged by size:
4486 1118 255 60 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1470 2028 3116 1470
Size: 558 1088 4431
Fragments arranged by size:
4431 1088 558
KasI G'GCGC_C
Cuts at: 3507 3755 3507
Size: 248 5829
KpnI G_GTAC'C
Cuts at: 3097 3097
Size: 6077
MluI A'CGCG_T
Cuts at: 5510 5510
Size: 6077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2132 2939 4836 5020 2051
Size: 81 807 1897 184 3108
Fragments arranged by size:
3108 1897 807 184 81
MscI TGG'CCA
Cuts at: 296 748 1528 1615 1804 2357 3838 296
Size: 452 780 87 189 553 1481 2535
Fragments arranged by size:
2535 1481 780 553 452 189 87
MslI CAynn'nnrTG
Cuts at: 337 790 859 1418 1534 1564 1615 1735 1786
Size: 453 69 559 116 30 51 120 51
Cuts at: 1786 4066 4193 337
Size: 2280 127 2221
Fragments arranged by size:
2280 2221 559 453 127 120 116 69
51 51 30
MspA1I CmG'CkG
Cuts at: 503 1447 1735 2535 2589 3188 3502 3862 4646
Size: 944 288 800 54 599 314 360 784
Cuts at: 4646 4891 503
Size: 245 1689
Fragments arranged by size:
1689 944 800 784 599 360 314 288
245 54
NaeI GCC'GGC
Cuts at: 2218 4258 2218
Size: 2040 4037
NarI GG'CG_CC
Cuts at: 3508 3756 3508
Size: 248 5829
NcoI C'CATG_G
Cuts at: 107 107
Size: 6077
NdeI CA'TA_TG
Cuts at: 1971 1971
Size: 6077
NgoMIV G'CCGG_C
Cuts at: 2216 4256 2216
Size: 2040 4037
NspI r_CATG'y
Cuts at: 2499 3130 3630 5233 2499
Size: 631 500 1603 3343
Fragments arranged by size:
3343 1603 631 500
PciI A'CATG_T
Cuts at: 2495 5229 2495
Size: 2734 3343
PflMI CCAn_nnn'nTGG
Cuts at: 423 2136 2592 423
Size: 1713 456 3908
Fragments arranged by size:
3908 1713 456
PfoI T'CCnGG_A
Cuts at: 1287 1287
Size: 6077
PmeI GTTT'AAAC
Cuts at: 3076 3076
Size: 6077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4523 4555 4523
Size: 32 6045
PspOMI G'GGCC_C
Cuts at: 804 3021 804
Size: 2217 3860
PstI C_TGCA'G
Cuts at: 587 1829 2081 2093 2391 2820 3124 3809 587
Size: 1242 252 12 298 429 304 685 2855
Fragments arranged by size:
2855 1242 685 429 304 298 252 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6077
PvuII CAG'CTG
Cuts at: 503 1447 1735 2535 2589 3502 3862 503
Size: 944 288 800 54 913 360 2718
Fragments arranged by size:
2718 944 913 800 360 288 54
RsrII CG'GwC_CG
Cuts at: 4272 4272
Size: 6077
SacI G_AGCT'C
Cuts at: 2445 3091 2445
Size: 646 5431
SalI G'TCGA_C
Cuts at: 3114 3114
Size: 6077
SapI GCTCTTCn'nnn_
Cuts at: 2327 4100 4310 2327
Size: 1773 210 4094
Fragments arranged by size:
4094 1773 210
SbfI CC_TGCA'GG
Cuts at: 2391 3124 2391
Size: 733 5344
ScaI AGT'ACT
Cuts at: 1383 5594 1383
Size: 4211 1866
SfcI C'TryA_G
Cuts at: 33 583 1825 2077 2089 2387 2704 2816 3120
Size: 550 1242 252 12 298 317 112 304
Cuts at: 3120 3805 4773 4964 33
Size: 685 968 191 1146
Fragments arranged by size:
1242 1146 968 685 550 317 304 298
252 191 112 12
SfoI GGC'GCC
Cuts at: 3509 3757 3509
Size: 248 5829
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6077
SmaI CCC'GGG
Cuts at: 1465 3099 1465
Size: 1634 4443
SmlI C'TyrA_G
Cuts at: 3232 4147 4861 5123 3232
Size: 915 714 262 4186
Fragments arranged by size:
4186 915 714 262
SpeI A'CTAG_T
Cuts at: 67 612 67
Size: 545 5532
SphI G_CATG'C
Cuts at: 3130 3630 3130
Size: 500 5577
StuI AGG'CCT
Cuts at: 541 2518 2847 541
Size: 1977 329 3771
Fragments arranged by size:
3771 1977 329
StyI C'CwwG_G
Cuts at: 107 749 1884 2637 3211 3565 107
Size: 642 1135 753 574 354 2619
Fragments arranged by size:
2619 1135 753 642 574 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1395 3660 3793 4252 4461 1395
Size: 2265 133 459 209 3011
Fragments arranged by size:
3011 2265 459 209 133
TatI w'GTAC_w
Cuts at: 1381 5592 1381
Size: 4211 1866
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 419 451 1164 1196 1711 1743 5594 5626 419
Size: 32 713 32 515 32 3851 32 870
Fragments arranged by size:
3851 870 713 515 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 517 3874 517
Size: 3357 2720
XbaI T'CTAG_A
Cuts at: 3108 3108
Size: 6077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 366 1119 2484 3572 366
Size: 753 1365 1088 2871
Fragments arranged by size:
2871 1365 1088 753
XmaI C'CCGG_G
Cuts at: 1463 3097 1463
Size: 1634 4443
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AloI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BstZ17I ClaI DraIII FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI