Restriction map for
pF1KB7292
(Circular) (Six-base) MAPSORT of: pF1KB7292.seq Check: 1748 from: 1 to: 6695
pF1KB7292 6695 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1798 3338 1798
Size: 1540 5155
AccI GT'mk_AC
Cuts at: 2333 3733 2333
Size: 1400 5295
Acc65I G'GTAC_C
Cuts at: 3711 3711
Size: 6695
AclI AA'CG_TT
Cuts at: 768 6535 768
Size: 5767 928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 3213 4520 4952 5699 5967 1589
Size: 1624 1307 432 747 268 2317
Fragments arranged by size:
2317 1624 1307 747 432 268
AfeI AGC'GCT
Cuts at: 214 214
Size: 6695
AflIII A'CryG_T
Cuts at: 359 3434 4047 5151 6128 359
Size: 3075 613 1104 977 926
Fragments arranged by size:
3075 1104 977 926 613
AgeI A'CCGG_T
Cuts at: 521 5083 521
Size: 4562 2133
AhdI GACnn_n'nnGTC
Cuts at: 540 540
Size: 6695
AleI CACnn'nnGTG
Cuts at: 1969 1969
Size: 6695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1009 1471 3604 1009
Size: 462 2133 4100
Fragments arranged by size:
4100 2133 462
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 337 369 3116 3148 337
Size: 32 2747 32 3884
Fragments arranged by size:
3884 2747 32 32
AlwNI CAG_nnn'CTG
Cuts at: 627 3330 3339 4000 5567 627
Size: 2703 9 661 1567 1755
Fragments arranged by size:
2703 1755 1567 661 9
ApaLI G'TGCA_C
Cuts at: 1591 2851 3040 5465 1591
Size: 1260 189 2425 2821
Fragments arranged by size:
2821 2425 1260 189
ApoI r'AATT_y
Cuts at: 3699 6048 6304 3699
Size: 2349 256 4090
Fragments arranged by size:
4090 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6695
AvaI C'yCGr_G
Cuts at: 295 3280 3576 3715 295
Size: 2985 296 139 3275
Fragments arranged by size:
3275 2985 296 139
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2304 2337 3216 3249 2304
Size: 33 879 33 5750
Fragments arranged by size:
5750 879 33 33
BamHI G'GATC_C
Cuts at: 3720 3720
Size: 6695
BanI G'GyrC_C
Cuts at: 1019 2814 2837 3588 3711 4125 4373 4408 1019
Size: 1795 23 751 123 414 248 35 3306
Fragments arranged by size:
3306 1795 751 414 248 123 35 23
BanII G_rGCy'C
Cuts at: 119 255 1847 1997 2223 3709 4739 119
Size: 136 1592 150 226 1486 1030 2075
Fragments arranged by size:
2075 1592 1486 1030 226 150 136
BbeI G_GCGC'C
Cuts at: 1023 4129 4377 1023
Size: 3106 248 3341
Fragments arranged by size:
3341 3106 248
BbsI GAAGACnn'nnnn_
Cuts at: 2866 3476 2866
Size: 610 6085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 185 219 1113 1147 185
Size: 34 894 34 5733
Fragments arranged by size:
5733 894 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3648 4593 5354 6695 3648
Size: 945 761 1341 3648
Fragments arranged by size:
3648 1341 945 761
BclI T'GATC_A
Cuts at: 2228 2279 4215 2228
Size: 51 1936 4708
Fragments arranged by size:
4708 1936 51
BfrBI ATG'CAT
Cuts at: 2682 2682
Size: 6695
BglI GCCn_nnn'nGGC
Cuts at: 1415 1415
Size: 6695
BglII A'GATC_T
Cuts at: 1827 2183 4210 6247 1827
Size: 356 2027 2037 2275
Fragments arranged by size:
2275 2037 2027 356
BlpI GC'TnA_GC
Cuts at: 3352 3807 3352
Size: 455 6240
Bme1580I G_kGCm'C
Cuts at: 296 1595 2041 2441 2516 2819 2855 3044 3593
Size: 1299 446 400 75 303 36 189 549
Cuts at: 3593 4320 4413 5469 296
Size: 727 93 1056 1522
Fragments arranged by size:
1522 1299 1056 727 549 446 400 303
189 93 75 36
BmrI ACTGGGnnnn_n'
Cuts at: 3907 3988 4323 6467 3907
Size: 81 335 2144 4135
Fragments arranged by size:
4135 2144 335 81
BmtI G_CTAG'C
Cuts at: 2506 2506
Size: 6695
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3393 5062 3393
Size: 1669 5026
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 616 1124 1517 1968 2068 3142 3193 3409
Size: 34 508 393 451 100 1074 51 216
Cuts at: 3409 582
Size: 3868
Fragments arranged by size:
3868 1074 508 451 393 216 100 51
34
Bpu10I CC'TnA_GC
Cuts at: 1006 2222 1006
Size: 1216 5479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1885 3871 4750 5242 5540 1885
Size: 1986 879 492 298 3040
Fragments arranged by size:
3040 1986 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3097 3381 6399 3097
Size: 284 3018 3393
Fragments arranged by size:
3393 3018 284
BsaAI yAC'GTr
Cuts at: 1413 2856 3435 4048 1413
Size: 1443 579 613 4060
Fragments arranged by size:
4060 1443 613 579
BsaBI GATnn'nnATC
Cuts at: 6 2614 4209 6246 6
Size: 2608 1595 2037 455
Fragments arranged by size:
2608 2037 1595 455
BsaHI Gr'CG_yC
Cuts at: 1020 1028 3544 4126 4374 5076 6572 1020
Size: 8 2516 582 248 702 1496 1143
Fragments arranged by size:
2516 1496 1143 702 582 248 8
BsaWI w'CCGG_w
Cuts at: 521 3884 4405 5083 5357 5504 5915 521
Size: 3363 521 678 274 147 411 1301
Fragments arranged by size:
3363 1301 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 337 367 1490 1495 1520 1525 2798 2828 3116
Size: 30 1123 5 25 5 1273 30 288
Cuts at: 3116 3146 3421 3451 337
Size: 30 275 30 3581
Fragments arranged by size:
3581 1273 1123 288 275 30 30 30
30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1516 2097 2383 2995 2998 3001 3115 3143 3382
Size: 581 286 612 3 3 114 28 239
Cuts at: 3382 3412 3499 3502 3505 1516
Size: 30 87 3 3 4706
Fragments arranged by size:
4706 612 581 286 239 114 87 30
28 3 3 3 3
BseYI C'CCAG_C
Cuts at: 135 901 2996 3592 4120 4878 5455 5951 135
Size: 766 2095 596 528 758 577 496 879
Fragments arranged by size:
2095 879 766 758 596 577 528 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1272 1698 2172 3606 640
Size: 632 426 474 1434 3729
Fragments arranged by size:
3729 1434 632 474 426
BsiEI CG_ry'CG
Cuts at: 104 284 4237 4283 5491 104
Size: 180 3953 46 1208 1308
Fragments arranged by size:
3953 1308 1208 180 46
BsiHKAI G_wGCw'C
Cuts at: 1595 1997 2855 3044 3358 3446 3620 3709 4487
Size: 402 858 189 314 88 174 89 778
Cuts at: 4487 4677 5469 1595
Size: 190 792 2821
Fragments arranged by size:
2821 858 792 778 402 314 190 189
174 89 88
BsmI GAATG_Cn'
Cuts at: 2766 2766
Size: 6695
BsmBI CGTCTCn'nnnn_
Cuts at: 212 1520 2863 212
Size: 1308 1343 4044
Fragments arranged by size:
4044 1343 1308
Bsp1286I G_dGCh'C
Cuts at: 119 255 296 1595 1847 1997 2041 2223 2441
Size: 136 41 1299 252 150 44 182 218
Cuts at: 2441 2516 2819 2855 3044 3358 3446 3593 3620
Size: 75 303 36 189 314 88 147 27
Cuts at: 3620 3709 4320 4413 4487 4677 4739 5469 119
Size: 89 611 93 74 190 62 730 1345
Fragments arranged by size:
1345 1299 730 611 314 303 252 218
190 189 182 150 147 136 93 89
88 75 74 62 44 41 36 27
BspEI T'CCGG_A
Cuts at: 5915 5915
Size: 6695
BspHI T'CATG_A
Cuts at: 1550 2207 2399 5871 1550
Size: 657 192 3472 2374
Fragments arranged by size:
3472 2374 657 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 635 1798 3338 3506 3745 3959 4261 4642 635
Size: 1163 1540 168 239 214 302 381 2688
Fragments arranged by size:
2688 1540 1163 381 302 239 214 168
BsrBI CCG'CTC
Cuts at: 50 824 2711 3450 4987 5041 6525 6690 50
Size: 774 1887 739 1537 54 1484 165 55
Fragments arranged by size:
1887 1537 1484 774 739 165 55 54
BsrDI GCAATG_nn'
Cuts at: 572 1302 2051 2231 4607 572
Size: 730 749 180 2376 2660
Fragments arranged by size:
2660 2376 749 730 180
BsrFI r'CCGG_y
Cuts at: 521 4693 4874 5083 521
Size: 4172 181 209 2133
Fragments arranged by size:
4172 2133 209 181
BsrGI T'GTAC_A
Cuts at: 698 2480 2612 698
Size: 1782 132 4781
Fragments arranged by size:
4781 1782 132
BssSI C'ACGA_G
Cuts at: 1194 3445 4966 5324 1194
Size: 2251 1521 358 2565
Fragments arranged by size:
2565 2251 1521 358
BstAPI GCAn_nnn'nTGC
Cuts at: 627 1386 1754 2761 3330 3339 4072 627
Size: 759 368 1007 569 9 733 3250
Fragments arranged by size:
3250 1007 759 733 569 368 9
BstBI TT'CG_AA
Cuts at: 5056 5056
Size: 6695
BstEII G'GTnAC_C
Cuts at: 2271 2271
Size: 6695
BstXI CCAn_nnnn'nTGG
Cuts at: 2358 2926 2358
Size: 568 6127
BstYI r'GATC_y
Cuts at: 1 419 1827 2183 3720 4187 4210 4545 4791
Size: 418 1408 356 1537 467 23 335 246
Cuts at: 4791 5803 5889 5901 6247 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1537 1408 1012 467 449 418 356 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 712 712
Size: 6695
BtgI C'CryG_G
Cuts at: 107 446 1364 3032 3386 107
Size: 339 918 1668 354 3416
Fragments arranged by size:
3416 1668 918 354 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1439 1881 2128 2705 4645 4826 4989 1439
Size: 442 247 577 1940 181 163 3145
Fragments arranged by size:
3145 1940 577 442 247 181 163
BtsI GCAGTG_nn'
Cuts at: 420 1686 2567 2655 3938 420
Size: 1266 881 88 1283 3177
Fragments arranged by size:
3177 1283 1266 881 88
DraI TTT'AAA
Cuts at: 1278 3694 1278
Size: 2416 4279
DraIII CAC_nnn'GTG
Cuts at: 3258 3258
Size: 6695
DrdI GACnn_nn'nnGTC
Cuts at: 238 3549 4401 5259 238
Size: 3311 852 858 1674
Fragments arranged by size:
3311 1674 858 852
EaeI y'GGCC_r
Cuts at: 221 281 537 1407 3119 3506 4280 4454 4845
Size: 60 256 870 1712 387 774 174 391
Cuts at: 4845 4872 6217 221
Size: 27 1345 699
Fragments arranged by size:
1712 1345 870 774 699 391 387 256
174 60 27
EagI C'GGCC_G
Cuts at: 281 4280 281
Size: 3999 2696
EarI CTCTTCn'nnn_
Cuts at: 262 447 643 904 3118 3476 3684 4718 4928
Size: 185 196 261 2214 358 208 1034 210
Cuts at: 4928 262
Size: 2029
Fragments arranged by size:
2214 2029 1034 358 261 210 208 196
185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3062 4903 5213 5359 6505 3062
Size: 1841 310 146 1146 3252
Fragments arranged by size:
3252 1841 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1995 3707 1995
Size: 1712 4983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 616 1124 1517 1589 1968 2068 3142 3193
Size: 34 508 393 72 379 100 1074 51
Cuts at: 3193 3213 3409 4520 4952 5699 5967 582
Size: 20 196 1111 432 747 268 1310
Fragments arranged by size:
1310 1111 1074 747 508 432 393 379
268 196 100 72 51 34 20
EcoNI CCTnn'n_nnAGG
Cuts at: 3136 3136
Size: 6695
EcoO109I rG'GnC_Cy
Cuts at: 3268 3495 3834 3268
Size: 227 339 6129
Fragments arranged by size:
6129 339 227
EcoRI G'AATT_C
Cuts at: 3699 3699
Size: 6695
EcoRV GAT'ATC
Cuts at: 1548 1548
Size: 6695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 4714 2353
Size: 2361 4334
FspI TGC'GCA
Cuts at: 931 4476 931
Size: 3545 3150
HaeII r_GCGC'y
Cuts at: 216 1013 1023 2091 4129 4377 5399 6378 216
Size: 797 10 1068 2038 248 1022 979 533
Fragments arranged by size:
2038 1068 1022 979 797 533 248 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 227 259 526 558 953 985 1067 1099 1135
Size: 32 267 32 395 32 82 32 36
Cuts at: 1135 1167 2381 2413 4537 4567 4569 4599 227
Size: 32 1214 32 2124 30 2 30 2323
Fragments arranged by size:
2323 2124 1214 395 267 82 36 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3734 3734
Size: 6695
HindIII A'AGCT_T
Cuts at: 1302 1796 1302
Size: 494 6201
KasI G'GCGC_C
Cuts at: 1019 4125 4373 1019
Size: 3106 248 3341
Fragments arranged by size:
3341 3106 248
KpnI G_GTAC'C
Cuts at: 3715 3715
Size: 6695
MfeI C'AATT_G
Cuts at: 774 774
Size: 6695
MluI A'CGCG_T
Cuts at: 6128 6128
Size: 6695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 1578 2738 5366 5550 344
Size: 1234 1160 2628 184 1489
Fragments arranged by size:
2628 1489 1234 1160 184
MscI TGG'CCA
Cuts at: 223 1409 4456 223
Size: 1186 3047 2462
Fragments arranged by size:
3047 2462 1186
MslI CAynn'nnrTG
Cuts at: 616 991 1852 1969 3037 4684 4811 616
Size: 375 861 117 1068 1647 127 2500
Fragments arranged by size:
2500 1647 1068 861 375 127 117
MspA1I CmG'CkG
Cuts at: 3345 3351 3388 3806 4120 4480 5493 5738 3345
Size: 6 37 418 314 360 1013 245 4302
Fragments arranged by size:
4302 1013 418 360 314 245 37 6
NaeI GCC'GGC
Cuts at: 4876 4876
Size: 6695
NarI GG'CG_CC
Cuts at: 1020 4126 4374 1020
Size: 3106 248 3341
Fragments arranged by size:
3341 3106 248
NcoI C'CATG_G
Cuts at: 107 1364 3032 107
Size: 1257 1668 3770
Fragments arranged by size:
3770 1668 1257
NdeI CA'TA_TG
Cuts at: 2831 2831
Size: 6695
NgoMIV G'CCGG_C
Cuts at: 4874 4874
Size: 6695
NheI G'CTAG_C
Cuts at: 2502 2502
Size: 6695
NsiI A_TGCA'T
Cuts at: 2684 2684
Size: 6695
NspI r_CATG'y
Cuts at: 1389 2598 2682 2686 3748 4248 5155 1389
Size: 1209 84 4 1062 500 907 2929
Fragments arranged by size:
2929 1209 1062 907 500 84 4
PasI CC'CwG_GG
Cuts at: 408 408
Size: 6695
PciI A'CATG_T
Cuts at: 5151 5151
Size: 6695
PflMI CCAn_nnn'nTGG
Cuts at: 342 363 3216 342
Size: 21 2853 3821
Fragments arranged by size:
3821 2853 21
PfoI T'CCnGG_A
Cuts at: 422 1248 1322 1533 3242 3425 422
Size: 826 74 211 1709 183 3692
Fragments arranged by size:
3692 1709 826 211 183 74
PmeI GTTT'AAAC
Cuts at: 3694 3694
Size: 6695
PmlI CAC'GTG
Cuts at: 1413 2856 1413
Size: 1443 5252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 337 369 3116 3148 5834 5866 337
Size: 32 2747 32 2686 32 1166
Fragments arranged by size:
2747 2686 1166 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3495 3495
Size: 6695
PshAI GACnn'nnGTC
Cuts at: 784 784
Size: 6695
PstI C_TGCA'G
Cuts at: 795 1136 1382 1562 3335 3350 3503 3742 4427
Size: 341 246 180 1773 15 153 239 685
Cuts at: 4427 795
Size: 3063
Fragments arranged by size:
3063 1773 685 341 246 239 180 153
15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6695
PvuII CAG'CTG
Cuts at: 3351 4120 4480 3351
Size: 769 360 5566
Fragments arranged by size:
5566 769 360
RsrII CG'GwC_CG
Cuts at: 4890 4890
Size: 6695
SacI G_AGCT'C
Cuts at: 1997 3709 1997
Size: 1712 4983
SacII CC_GC'GG
Cuts at: 3389 3389
Size: 6695
SalI G'TCGA_C
Cuts at: 3732 3732
Size: 6695
SapI GCTCTTCn'nnn_
Cuts at: 643 904 3684 4718 4928 643
Size: 261 2780 1034 210 2410
Fragments arranged by size:
2780 2410 1034 261 210
SbfI CC_TGCA'GG
Cuts at: 3503 3742 3503
Size: 239 6456
ScaI AGT'ACT
Cuts at: 6212 6212
Size: 6695
SexAI A'CCwGG_T
Cuts at: 2799 2799
Size: 6695
SfcI C'TryA_G
Cuts at: 33 605 791 891 1132 1378 1558 3331 3346
Size: 572 186 100 241 246 180 1773 15
Cuts at: 3346 3499 3738 4423 5416 5607 33
Size: 153 239 685 993 191 1121
Fragments arranged by size:
1773 1121 993 685 572 246 241 239
191 186 180 153 100 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 1415 1415
Size: 6695
SfoI GGC'GCC
Cuts at: 1021 4127 4375 1021
Size: 3106 248 3341
Fragments arranged by size:
3341 3106 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6695
SmaI CCC'GGG
Cuts at: 3282 3717 3282
Size: 435 6260
SmlI C'TyrA_G
Cuts at: 1864 3850 4765 5257 5519 1864
Size: 1986 915 492 262 3040
Fragments arranged by size:
3040 1986 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6695
SphI G_CATG'C
Cuts at: 2682 3748 4248 2682
Size: 1066 500 5129
Fragments arranged by size:
5129 1066 500
StuI AGG'CCT
Cuts at: 863 863
Size: 6695
StyI C'CwwG_G
Cuts at: 107 953 1023 1364 2025 2819 2919 3032 3137
Size: 846 70 341 661 794 100 113 105
Cuts at: 3137 3829 4183 107
Size: 692 354 2619
Fragments arranged by size:
2619 846 794 692 661 354 341 113
105 100 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 577 935 2350 4278 4411 4870 5079 577
Size: 358 1415 1928 133 459 209 2193
Fragments arranged by size:
2193 1928 1415 459 358 209 133
TatI w'GTAC_w
Cuts at: 698 1562 1950 2480 2612 6210 698
Size: 864 388 530 132 3598 1183
Fragments arranged by size:
3598 1183 864 530 388 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1185 1217 1490 1522 2798 2830 6212 6244 1185
Size: 32 273 32 1276 32 3382 32 1636
Fragments arranged by size:
3382 1636 1276 273 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 385 760 4492 385
Size: 375 3732 2588
Fragments arranged by size:
3732 2588 375
XbaI T'CTAG_A
Cuts at: 3726 3726
Size: 6695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 405 960 1362 4190 405
Size: 555 402 2828 2910
Fragments arranged by size:
2910 2828 555 402
XmaI C'CCGG_G
Cuts at: 3280 3715 3280
Size: 435 6260
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII ApaI AscI AvrII BbvCI BmgBI BsiWI
BssHII BstZ17I ClaI CspCI FseI FspAI HpaI NotI
NruI PacI PsiI PspOMI PspXI PsrI SanDI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI