Restriction map for
pF1KB7293
(Circular) (Six-base) MAPSORT of: pF1KB7293.seq Check: 2488 from: 1 to: 6203
pF1KB7293 6203 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 413 2991 413
Size: 2578 3625
AccI GT'mk_AC
Cuts at: 3241 3241
Size: 6203
Acc65I G'GTAC_C
Cuts at: 3219 3219
Size: 6203
AclI AA'CG_TT
Cuts at: 6043 6043
Size: 6203
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1044 1725 4028 4460 4813 5475 1044
Size: 681 2303 432 353 662 1772
Fragments arranged by size:
2303 1772 681 662 432 353
AflIII A'CryG_T
Cuts at: 2450 3555 5355 5636 2450
Size: 1105 1800 281 3017
Fragments arranged by size:
3017 1800 1105 281
AhdI GACnn_n'nnGTC
Cuts at: 3145 3145
Size: 6203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 421 421
Size: 6203
AlwNI CAG_nnn'CTG
Cuts at: 969 2043 2545 3508 4946 969
Size: 1074 502 963 1438 2226
Fragments arranged by size:
2226 1438 1074 963 502
ApaI G_GGCC'C
Cuts at: 745 2060 745
Size: 1315 4888
ApaLI G'TGCA_C
Cuts at: 1395 5041 1395
Size: 3646 2557
ApoI r'AATT_y
Cuts at: 3207 5556 5812 3207
Size: 2349 256 3598
Fragments arranged by size:
3598 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6203
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6203
AvaI C'yCGr_G
Cuts at: 496 2296 2843 3035 3223 496
Size: 1800 547 192 188 3476
Fragments arranged by size:
3476 1800 547 192 188
BamHI G'GATC_C
Cuts at: 3228 3228
Size: 6203
BanI G'GyrC_C
Cuts at: 297 475 712 858 1095 1120 1348 2095 2527
Size: 178 237 146 237 25 228 747 432
Cuts at: 2527 3219 3633 3881 3916 297
Size: 692 414 248 35 2584
Fragments arranged by size:
2584 747 692 432 414 248 237 237
228 178 146 35 25
BanII G_rGCy'C
Cuts at: 200 344 745 760 1622 1682 1948 2060 2174
Size: 144 401 15 862 60 266 112 114
Cuts at: 2174 3217 4247 200
Size: 1043 1030 2156
Fragments arranged by size:
2156 1043 1030 862 401 266 144 114
112 60 15
BbeI G_GCGC'C
Cuts at: 3637 3885 3637
Size: 248 5955
BbsI GAAGACnn'nnnn_
Cuts at: 1536 2018 2378 2626 1536
Size: 482 360 248 5113
Fragments arranged by size:
5113 482 360 248
BbvCI CC'TCA_GC
Cuts at: 1981 2113 1981
Size: 132 6071
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1110 1144 1110
Size: 34 6169
BciVI GTATCCnnnnn_n'
Cuts at: 4101 5157 6203 4101
Size: 1056 1046 4101
Fragments arranged by size:
4101 1056 1046
BclI T'GATC_A
Cuts at: 1475 3723 1475
Size: 2248 3955
BglI GCCn_nnn'nGGC
Cuts at: 1104 1421 1104
Size: 317 5886
BglII A'GATC_T
Cuts at: 2954 3718 5755 2954
Size: 764 2037 3402
Fragments arranged by size:
3402 2037 764
BlpI GC'TnA_GC
Cuts at: 184 1676 2782 2849 3315 184
Size: 1492 1106 67 466 3072
Fragments arranged by size:
3072 1492 1106 466 67
Bme1580I G_kGCm'C
Cuts at: 302 745 861 1399 2060 2098 2195 3091 3828
Size: 443 116 538 661 38 97 896 737
Cuts at: 3828 3921 5045 302
Size: 93 1124 1460
Fragments arranged by size:
1460 1124 896 737 661 538 443 116
97 93 38
BmrI ACTGGGnnnn_n'
Cuts at: 2031 2806 3415 3496 3831 5975 2031
Size: 775 609 81 335 2144 2259
Fragments arranged by size:
2259 2144 775 609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4570 4570
Size: 6203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 2158 3128 3136 829
Size: 1329 970 8 3896
Fragments arranged by size:
3896 1329 970 8
Bpu10I CC'TnA_GC
Cuts at: 1981 2113 2168 1981
Size: 132 55 6016
Fragments arranged by size:
6016 132 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1183 3379 4258 4972 5270 370
Size: 813 2196 879 714 298 1303
Fragments arranged by size:
2196 1303 879 813 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 820 909 996 1776 2299 3112 5907 820
Size: 89 87 780 523 813 2795 1116
Fragments arranged by size:
2795 1116 813 780 523 89 87
BsaAI yAC'GTr
Cuts at: 2136 3556 2136
Size: 1420 4783
BsaBI GATnn'nnATC
Cuts at: 6 628 3717 5754 6
Size: 622 3089 2037 455
Fragments arranged by size:
3089 2037 622 455
BsaHI Gr'CG_yC
Cuts at: 133 143 2267 3634 3882 4584 6080 133
Size: 10 2124 1367 248 702 1496 256
Fragments arranged by size:
2124 1496 1367 702 256 248 10
BsaWI w'CCGG_w
Cuts at: 3392 3913 4591 5002 5149 5423 3392
Size: 521 678 411 147 274 4172
Fragments arranged by size:
4172 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 937 967 1030 1060 3109 3139 361
Size: 30 546 30 63 30 2049 30 3425
Fragments arranged by size:
3425 2049 546 63 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 514 1497 1846 1938 2593 2656 2709 2980 514
Size: 983 349 92 655 63 53 271 3737
Fragments arranged by size:
3737 983 655 349 271 92 63 53
BseYI C'CCAG_C
Cuts at: 232 329 433 518 1343 1400 2493 3091 3628
Size: 97 104 85 825 57 1093 598 537
Cuts at: 3628 4386 5051 5459 232
Size: 758 665 408 976
Fragments arranged by size:
1093 976 825 758 665 598 537 408
104 97 85 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 358 174
Size: 184 6019
BsiEI CG_ry'CG
Cuts at: 104 290 3745 3791 5021 104
Size: 186 3455 46 1230 1286
Fragments arranged by size:
3455 1286 1230 186 46
BsiHKAI G_wGCw'C
Cuts at: 314 1399 1682 1898 2051 2141 2174 2855 3182
Size: 1085 283 216 153 90 33 681 327
Cuts at: 3182 3217 3995 4185 5045 314
Size: 35 778 190 860 1472
Fragments arranged by size:
1472 1085 860 778 681 327 283 216
190 153 90 35 33
BsmBI CGTCTCn'nnnn_
Cuts at: 124 3032 124
Size: 2908 3295
Bsp1286I G_dGCh'C
Cuts at: 200 302 314 344 745 760 861 1399 1622
Size: 102 12 30 401 15 101 538 223
Cuts at: 1622 1682 1898 1948 2051 2060 2098 2141 2174
Size: 60 216 50 103 9 38 43 33
Cuts at: 2174 2195 2855 3091 3182 3217 3828 3921 3995
Size: 21 660 236 91 35 611 93 74
Cuts at: 3995 4185 4247 5045 200
Size: 190 62 798 1358
Fragments arranged by size:
1358 798 660 611 538 401 236 223
216 190 103 102 101 93 91 74
62 60 50 43 38 35 33 30
21 15 12 9
BspHI T'CATG_A
Cuts at: 2426 4635 2426
Size: 2209 3994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 380 413 584 848 1643 2281 2991 3253 3467
Size: 33 171 264 795 638 710 262 214
Cuts at: 3467 3769 4150 380
Size: 302 381 2433
Fragments arranged by size:
2433 795 710 638 381 302 264 262
214 171 33
BsrBI CCG'CTC
Cuts at: 50 4495 4549 6033 6198 50
Size: 4445 54 1484 165 55
Fragments arranged by size:
4445 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 893 2243 4115 893
Size: 1350 1872 2981
Fragments arranged by size:
2981 1872 1350
BsrFI r'CCGG_y
Cuts at: 428 4201 4382 428
Size: 3773 181 2249
Fragments arranged by size:
3773 2249 181
BsrGI T'GTAC_A
Cuts at: 3101 3101
Size: 6203
BssSI C'ACGA_G
Cuts at: 439 4474 5182 439
Size: 4035 708 1460
Fragments arranged by size:
4035 1460 708
BstAPI GCAn_nnn'nTGC
Cuts at: 260 2163 3580 260
Size: 1903 1417 2883
Fragments arranged by size:
2883 1903 1417
BstBI TT'CG_AA
Cuts at: 4564 4564
Size: 6203
BstEII G'GTnAC_C
Cuts at: 648 1192 648
Size: 544 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 792 853 933 1452 1656 1890 2319 2568 792
Size: 61 80 519 204 234 429 249 4427
Fragments arranged by size:
4427 519 429 249 234 204 80 61
BstYI r'GATC_y
Cuts at: 1 2273 2714 2954 3228 3695 3718 4053 4299
Size: 2272 441 240 274 467 23 335 246
Cuts at: 4299 4605 4617 4703 5755 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2272 1052 467 449 441 335 306 274
246 240 86 23 12
BtgI C'CryG_G
Cuts at: 107 791 899 1838 107
Size: 684 108 939 4472
Fragments arranged by size:
4472 939 684 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1635 1656 1863 2166 4153 4334 4497 1635
Size: 21 207 303 1987 181 163 3341
Fragments arranged by size:
3341 1987 303 207 181 163 21
BtsI GCAGTG_nn'
Cuts at: 178 195 3446 178
Size: 17 3251 2935
Fragments arranged by size:
3251 2935 17
ClaI AT'CG_AT
Cuts at: 290 290
Size: 6203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 690 725 690
Size: 35 6168
DraI TTT'AAA
Cuts at: 3202 3202
Size: 6203
DraIII CAC_nnn'GTG
Cuts at: 152 813 1394 1865 2201 152
Size: 661 581 471 336 4154
Fragments arranged by size:
4154 661 581 471 336
DrdI GACnn_nn'nnGTC
Cuts at: 1081 3909 5253 1081
Size: 2828 1344 2031
Fragments arranged by size:
2828 2031 1344
EaeI y'GGCC_r
Cuts at: 800 923 1157 1238 1268 2130 2360 2685 2733
Size: 123 234 81 30 862 230 325 48
Cuts at: 2733 3788 3962 4353 4380 5725 800
Size: 1055 174 391 27 1345 1278
Fragments arranged by size:
1345 1278 1055 862 391 325 234 230
174 123 81 48 30 27
EagI C'GGCC_G
Cuts at: 3788 3788
Size: 6203
EarI CTCTTCn'nnn_
Cuts at: 1903 4226 4436 1903
Size: 2323 210 3670
Fragments arranged by size:
3670 2323 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1087 2059 4411 5153 5299 6013 1087
Size: 972 2352 742 146 714 1277
Fragments arranged by size:
2352 1277 972 742 714 146
EcoICRI GAG'CTC
Cuts at: 1680 2172 3215 1680
Size: 492 1043 4668
Fragments arranged by size:
4668 1043 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1044 1725 2158 3128 3136 4028 4460 4813
Size: 215 681 433 970 8 892 432 353
Cuts at: 4813 5475 829
Size: 662 1557
Fragments arranged by size:
1557 970 892 681 662 433 432 353
215 8
EcoNI CCTnn'n_nnAGG
Cuts at: 388 844 1558 2002 2281 2572 2998 3127 388
Size: 456 714 444 279 291 426 129 3464
Fragments arranged by size:
3464 714 456 444 426 291 279 129
EcoO109I rG'GnC_Cy
Cuts at: 837 1203 1229 1317 2056 2057 2253 2565 3132
Size: 366 26 88 739 1 196 312 567
Cuts at: 3132 3342 837
Size: 210 3698
Fragments arranged by size:
3698 739 567 366 312 210 196 88
26 1
EcoRI G'AATT_C
Cuts at: 3207 3207
Size: 6203
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4222 4222
Size: 6203
FspI TGC'GCA
Cuts at: 268 3984 268
Size: 3716 2487
FspAI rTGC'GCAy
Cuts at: 268 268
Size: 6203
HaeII r_GCGC'y
Cuts at: 165 1819 2522 2552 3637 3885 5115 5886 165
Size: 1654 703 30 1085 248 1230 771 482
Fragments arranged by size:
1654 1230 1085 771 703 482 248 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 361 393 937 969 1030 1062 2760
Size: 32 145 32 544 32 61 32 1698
Cuts at: 2760 2792 3109 3131 3141 3163 4045 4075 4077
Size: 32 317 22 10 22 882 30 2
Cuts at: 4077 4107 184
Size: 30 2280
Fragments arranged by size:
2280 1698 882 544 317 145 61 32
32 32 32 32 30 30 22 22
10 2
HincII GTy'rAC
Cuts at: 2925 3242 2925
Size: 317 5886
KasI G'GCGC_C
Cuts at: 3633 3881 3633
Size: 248 5955
KpnI G_GTAC'C
Cuts at: 3223 3223
Size: 6203
MluI A'CGCG_T
Cuts at: 5636 5636
Size: 6203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1730 2585 2817 4962 5146 1730
Size: 855 232 2145 184 2787
Fragments arranged by size:
2787 2145 855 232 184
MscI TGG'CCA
Cuts at: 802 925 1240 1270 2132 3964 802
Size: 123 315 30 862 1832 3041
Fragments arranged by size:
3041 1832 862 315 123 30
MslI CAynn'nnrTG
Cuts at: 407 898 1450 1654 1837 1888 2320 2431 4192
Size: 491 552 204 183 51 432 111 1761
Cuts at: 4192 4319 407
Size: 127 2291
Fragments arranged by size:
2291 1761 552 491 432 204 183 127
111 51
MspA1I CmG'CkG
Cuts at: 158 211 1412 1569 1745 2497 3314 3628 3988
Size: 53 1201 157 176 752 817 314 360
Cuts at: 3988 4772 5017 158
Size: 784 245 1344
Fragments arranged by size:
1344 1201 817 784 752 360 314 245
176 157 53
NaeI GCC'GGC
Cuts at: 430 4384 430
Size: 3954 2249
NarI GG'CG_CC
Cuts at: 3634 3882 3634
Size: 248 5955
NcoI C'CATG_G
Cuts at: 107 107
Size: 6203
NdeI CA'TA_TG
Cuts at: 306 306
Size: 6203
NgoMIV G'CCGG_C
Cuts at: 428 4382 428
Size: 3954 2249
NspI r_CATG'y
Cuts at: 267 412 549 3256 3756 5359 267
Size: 145 137 2707 500 1603 1111
Fragments arranged by size:
2707 1603 1111 500 145 137
PciI A'CATG_T
Cuts at: 5355 5355
Size: 6203
PflMI CCAn_nnn'nTGG
Cuts at: 751 1092 1247 2043 751
Size: 341 155 796 4911
Fragments arranged by size:
4911 796 341 155
PfoI T'CCnGG_A
Cuts at: 730 845 730
Size: 115 6088
PmeI GTTT'AAAC
Cuts at: 3202 3202
Size: 6203
PmlI CAC'GTG
Cuts at: 2136 2136
Size: 6203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 4649
Size: 32 6171
PpuMI rG'GwC_Cy
Cuts at: 837 1203 1229 1317 2253 3132 837
Size: 366 26 88 936 879 3908
Fragments arranged by size:
3908 936 879 366 88 26
PspOMI G'GGCC_C
Cuts at: 741 2056 741
Size: 1315 4888
PstI C_TGCA'G
Cuts at: 1372 3250 3935 1372
Size: 1878 685 3640
Fragments arranged by size:
3640 1878 685
PvuI CG_AT'CG
Cuts at: 104 290 104
Size: 186 6017
PvuII CAG'CTG
Cuts at: 211 1412 1569 1745 3628 3988 211
Size: 1201 157 176 1883 360 2426
Fragments arranged by size:
2426 1883 1201 360 176 157
RsrII CG'GwC_CG
Cuts at: 4398 4398
Size: 6203
SacI G_AGCT'C
Cuts at: 1682 2174 3217 1682
Size: 492 1043 4668
Fragments arranged by size:
4668 1043 492
SalI G'TCGA_C
Cuts at: 3240 3240
Size: 6203
SanDI GG'GwC_CC
Cuts at: 1229 1229
Size: 6203
SapI GCTCTTCn'nnn_
Cuts at: 1903 4226 4436 1903
Size: 2323 210 3670
Fragments arranged by size:
3670 2323 210
SbfI CC_TGCA'GG
Cuts at: 3250 3250
Size: 6203
ScaI AGT'ACT
Cuts at: 5720 5720
Size: 6203
SexAI A'CCwGG_T
Cuts at: 1861 1861
Size: 6203
SfcI C'TryA_G
Cuts at: 33 1211 1368 3246 3931 4899 5090 33
Size: 1178 157 1878 685 968 191 1146
Fragments arranged by size:
1878 1178 1146 968 685 191 157
SfoI GGC'GCC
Cuts at: 3635 3883 3635
Size: 248 5955
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6203
SmaI CCC'GGG
Cuts at: 3225 3225
Size: 6203
SmlI C'TyrA_G
Cuts at: 385 1198 3358 4273 4987 5249 385
Size: 813 2160 915 714 262 1339
Fragments arranged by size:
2160 1339 915 813 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6203
SphI G_CATG'C
Cuts at: 267 412 549 3256 3756 267
Size: 145 137 2707 500 2714
Fragments arranged by size:
2714 2707 500 145 137
StuI AGG'CCT
Cuts at: 665 1045 2227 2657 665
Size: 380 1182 430 4211
Fragments arranged by size:
4211 1182 430 380
StyI C'CwwG_G
Cuts at: 107 956 1223 2561 2682 3337 3691 107
Size: 849 267 1338 121 655 354 2619
Fragments arranged by size:
2619 1338 849 655 354 267 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 343 630 784 876 897 1414 1766 3786 3919
Size: 287 154 92 21 517 352 2020 133
Cuts at: 3919 4378 4587 343
Size: 459 209 1959
Fragments arranged by size:
2020 1959 517 459 352 287 209 154
133 92 21
TatI w'GTAC_w
Cuts at: 3101 5718 3101
Size: 2617 3586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 5720 5752 2435
Size: 32 3253 32 2886
Fragments arranged by size:
3253 2886 32 32
Tth111I GACn'n_nGTC
Cuts at: 3123 4000 3123
Size: 877 5326
XbaI T'CTAG_A
Cuts at: 3234 3234
Size: 6203
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 810 1008 1167 1263 1937 2319 3698 810
Size: 198 159 96 674 382 1379 3315
Fragments arranged by size:
3315 1379 674 382 198 159 96
XmaI C'CCGG_G
Cuts at: 3223 3223
Size: 6203
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BspEI BssHII
BstZ17I Bsu36I EcoRV FseI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI