Restriction map for
pF1KB7294
(Circular) (Six-base) MAPSORT of: pF1KB7294.seq Check: 7537 from: 1 to: 5420
pF1KB7294 5420 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 568 1504 2143 568
Size: 936 639 3845
Fragments arranged by size:
3845 936 639
AccI GT'mk_AC
Cuts at: 1049 2458 1049
Size: 1409 4011
Acc65I G'GTAC_C
Cuts at: 2436 2436
Size: 5420
AclI AA'CG_TT
Cuts at: 5260 5260
Size: 5420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 2112 3245 3677 4030 4692 441
Size: 1671 1133 432 353 662 1169
Fragments arranged by size:
1671 1169 1133 662 432 353
AflIII A'CryG_T
Cuts at: 2081 2772 4572 4853 2081
Size: 691 1800 281 2648
Fragments arranged by size:
2648 1800 691 281
AhdI GACnn_n'nnGTC
Cuts at: 182 182
Size: 5420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1296 1296
Size: 5420
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 523 555 1312 1344 523
Size: 32 757 32 4599
Fragments arranged by size:
4599 757 32 32
AlwNI CAG_nnn'CTG
Cuts at: 306 570 1227 1239 1383 2116 2135 2725 4163
Size: 264 657 12 144 733 19 590 1438
Cuts at: 4163 306
Size: 1563
Fragments arranged by size:
1563 1438 733 657 590 264 144 19
12
ApaI G_GGCC'C
Cuts at: 343 343
Size: 5420
ApaLI G'TGCA_C
Cuts at: 808 4258 808
Size: 3450 1970
ApoI r'AATT_y
Cuts at: 2424 4773 5029 2424
Size: 2349 256 2815
Fragments arranged by size:
2815 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5420
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
AvaI C'yCGr_G
Cuts at: 1098 1754 2440 1098
Size: 656 686 4078
Fragments arranged by size:
4078 686 656
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2220 2253 2220
Size: 33 5387
BamHI G'GATC_C
Cuts at: 2445 2445
Size: 5420
BanI G'GyrC_C
Cuts at: 2302 2436 2850 3098 3133 2302
Size: 134 414 248 35 4589
Fragments arranged by size:
4589 414 248 134 35
BanII G_rGCy'C
Cuts at: 198 240 280 343 617 645 1076 1270 1574
Size: 42 40 63 274 28 431 194 304
Cuts at: 1574 1935 2434 3464 198
Size: 361 499 1030 2154
Fragments arranged by size:
2154 1030 499 431 361 304 274 194
63 42 40 28
BbeI G_GCGC'C
Cuts at: 2854 3102 2854
Size: 248 5172
BbsI GAAGACnn'nnnn_
Cuts at: 573 573
Size: 5420
BciVI GTATCCnnnnn_n'
Cuts at: 2248 3318 4374 5420 2248
Size: 1070 1056 1046 2248
Fragments arranged by size:
2248 1070 1056 1046
BclI T'GATC_A
Cuts at: 842 2940 842
Size: 2098 3322
BglI GCCn_nnn'nGGC
Cuts at: 745 745
Size: 5420
BglII A'GATC_T
Cuts at: 2935 4972 2935
Size: 2037 3383
BlpI GC'TnA_GC
Cuts at: 2532 2532
Size: 5420
Bme1580I G_kGCm'C
Cuts at: 343 812 1678 3045 3138 4262 343
Size: 469 866 1367 93 1124 1501
Fragments arranged by size:
1501 1367 1124 866 469 93
BmgBI CAC'GTC
Cuts at: 1893 2258 1893
Size: 365 5055
BmrI ACTGGGnnnn_n'
Cuts at: 735 1070 1334 2632 2713 3048 5192 735
Size: 335 264 1298 81 335 2144 963
Fragments arranged by size:
2144 1298 963 335 335 264 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3787 3787
Size: 5420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 430 515 1045 1949 212
Size: 218 85 530 904 3683
Fragments arranged by size:
3683 904 530 218 85
Bpu10I CC'TnA_GC
Cuts at: 1070 2044 1070
Size: 974 4446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 2596 3475 4189 4487 1408
Size: 1188 879 714 298 2341
Fragments arranged by size:
2341 1188 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 491 1021 1642 5124 491
Size: 530 621 3482 787
Fragments arranged by size:
3482 787 621 530
BsaAI yAC'GTr
Cuts at: 444 1140 2019 2773 444
Size: 696 879 754 3091
Fragments arranged by size:
3091 879 754 696
BsaBI GATnn'nnATC
Cuts at: 6 2934 4971 6
Size: 2928 2037 455
Fragments arranged by size:
2928 2037 455
BsaHI Gr'CG_yC
Cuts at: 1553 2851 3099 3801 5297 1553
Size: 1298 248 702 1496 1676
Fragments arranged by size:
1676 1496 1298 702 248
BsaWI w'CCGG_w
Cuts at: 674 1262 2404 2609 3130 3808 4219 4366 4640
Size: 588 1142 205 521 678 411 147 274
Cuts at: 4640 674
Size: 1454
Fragments arranged by size:
1454 1142 678 588 521 411 274 205
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 415 445 523 553 2003 2033 2399 2429 415
Size: 30 78 30 1450 30 366 30 3406
Fragments arranged by size:
3406 1450 366 78 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 423 676 740 1489 1900 1925 418
Size: 5 253 64 749 411 25 3913
Fragments arranged by size:
3913 749 411 253 64 25 5
BseYI C'CCAG_C
Cuts at: 125 218 222 256 378 476 1227 1302 1868
Size: 93 4 34 122 98 751 75 566
Cuts at: 1868 2296 2329 2372 2845 3603 4268 4676 125
Size: 428 33 43 473 758 665 408 869
Fragments arranged by size:
869 758 751 665 566 473 428 408
122 98 93 75 43 34 33 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1516 1524 1581 1638 1672 1899 2041 1030
Size: 486 8 57 57 34 227 142 4409
Fragments arranged by size:
4409 486 227 142 57 57 34 8
BsiEI CG_ry'CG
Cuts at: 104 763 2962 3008 4238 104
Size: 659 2199 46 1230 1286
Fragments arranged by size:
2199 1286 1230 659 46
BsiHKAI G_wGCw'C
Cuts at: 198 280 812 1120 1270 1935 2434 3212 3402
Size: 82 532 308 150 665 499 778 190
Cuts at: 3402 4262 198
Size: 860 1356
Fragments arranged by size:
1356 860 778 665 532 499 308 190
150 82
BsmBI CGTCTCn'nnnn_
Cuts at: 2269 2269
Size: 5420
Bsp1286I G_dGCh'C
Cuts at: 198 240 280 343 617 645 812 1076 1120
Size: 42 40 63 274 28 167 264 44
Cuts at: 1120 1270 1574 1678 1935 2434 3045 3138 3212
Size: 150 304 104 257 499 611 93 74
Cuts at: 3212 3402 3464 4262 198
Size: 190 62 798 1356
Fragments arranged by size:
1356 798 611 499 304 274 264 257
190 167 150 104 93 74 63 62
44 42 40 28
BspEI T'CCGG_A
Cuts at: 2404 2404
Size: 5420
BspHI T'CATG_A
Cuts at: 3852 3852
Size: 5420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 568 774 1343 1504 1660 1694 2143 2470 2684
Size: 206 569 161 156 34 449 327 214
Cuts at: 2684 2986 3367 568
Size: 302 381 2621
Fragments arranged by size:
2621 569 449 381 327 302 214 206
161 156 34
BsrBI CCG'CTC
Cuts at: 50 3712 3766 5250 5415 50
Size: 3662 54 1484 165 55
Fragments arranged by size:
3662 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2193 2324 3332 2193
Size: 131 1008 4281
Fragments arranged by size:
4281 1008 131
BsrFI r'CCGG_y
Cuts at: 758 1760 3418 3599 758
Size: 1002 1658 181 2579
Fragments arranged by size:
2579 1658 1002 181
BssSI C'ACGA_G
Cuts at: 1119 3691 4399 1119
Size: 2572 708 2140
Fragments arranged by size:
2572 2140 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1526 1535 1893 2069 2231 2797 1526
Size: 9 358 176 162 566 4149
Fragments arranged by size:
4149 566 358 176 162 9
BstBI TT'CG_AA
Cuts at: 3781 3781
Size: 5420
BstXI CCAn_nnnn'nTGG
Cuts at: 264 828 912 1298 2358 264
Size: 564 84 386 1060 3326
Fragments arranged by size:
3326 1060 564 386 84
BstYI r'GATC_y
Cuts at: 1 693 1001 1861 2445 2912 2935 3270 3516
Size: 692 308 860 584 467 23 335 246
Cuts at: 3516 3822 3834 3920 4972 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 860 692 584 467 449 335 308
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 242 647 242
Size: 405 5015
BtgI C'CryG_G
Cuts at: 911 974 1164 1600 2172 2267 911
Size: 63 190 436 572 95 4064
Fragments arranged by size:
4064 572 436 190 95 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 216 1216 1626 2083 3370 3551 3714 216
Size: 1000 410 457 1287 181 163 1922
Fragments arranged by size:
1922 1287 1000 457 410 181 163
BtsI GCAGTG_nn'
Cuts at: 501 1771 2151 2663 501
Size: 1270 380 512 3258
Fragments arranged by size:
3258 1270 512 380
DraI TTT'AAA
Cuts at: 2382 2419 2382
Size: 37 5383
DraIII CAC_nnn'GTG
Cuts at: 1606 1606
Size: 5420
DrdI GACnn_nn'nnGTC
Cuts at: 316 1061 3126 4470 316
Size: 745 2065 1344 1266
Fragments arranged by size:
2065 1344 1266 745
EaeI y'GGCC_r
Cuts at: 760 923 1205 3005 3179 3570 3597 4942 760
Size: 163 282 1800 174 391 27 1345 1238
Fragments arranged by size:
1800 1345 1238 391 282 174 163 27
EagI C'GGCC_G
Cuts at: 760 3005 760
Size: 2245 3175
EarI CTCTTCn'nnn_
Cuts at: 1099 1414 3443 3653 1099
Size: 315 2029 210 2866
Fragments arranged by size:
2866 2029 315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 310 778 2044 3628 4370 4516 5230 310
Size: 468 1266 1584 742 146 714 500
Fragments arranged by size:
1584 1266 742 714 500 468 146
EcoICRI GAG'CTC
Cuts at: 196 278 1268 1933 2432 196
Size: 82 990 665 499 3184
Fragments arranged by size:
3184 990 665 499 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 430 441 515 1045 1949 2112 3245 3677
Size: 218 11 74 530 904 163 1133 432
Cuts at: 3677 4030 4692 212
Size: 353 662 940
Fragments arranged by size:
1133 940 904 662 530 432 353 218
163 74 11
EcoNI CCTnn'n_nnAGG
Cuts at: 1940 1940
Size: 5420
EcoO109I rG'GnC_Cy
Cuts at: 176 184 339 652 1106 1947 2293 2559 176
Size: 8 155 313 454 841 346 266 3037
Fragments arranged by size:
3037 841 454 346 313 266 155 8
EcoRI G'AATT_C
Cuts at: 2424 2424
Size: 5420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3439 3439
Size: 5420
FspI TGC'GCA
Cuts at: 3201 3201
Size: 5420
HaeII r_GCGC'y
Cuts at: 126 453 2157 2854 3102 4332 5103 126
Size: 327 1704 697 248 1230 771 443
Fragments arranged by size:
1704 1230 771 697 443 327 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 168 200 1474 1506 3262 3292 3294 3324 168
Size: 32 1274 32 1756 30 2 30 2264
Fragments arranged by size:
2264 1756 1274 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1920 2459 1920
Size: 539 4881
KasI G'GCGC_C
Cuts at: 2850 3098 2850
Size: 248 5172
KpnI G_GTAC'C
Cuts at: 2440 2440
Size: 5420
MluI A'CGCG_T
Cuts at: 4853 4853
Size: 5420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4179 4363 4179
Size: 184 5236
MscI TGG'CCA
Cuts at: 3181 3181
Size: 5420
MslI CAynn'nnrTG
Cuts at: 3409 3536 3409
Size: 127 5293
MspA1I CmG'CkG
Cuts at: 222 306 858 1127 1403 1586 1730 2025 2300
Size: 84 552 269 276 183 144 295 275
Cuts at: 2300 2531 2845 3205 3989 4234 222
Size: 231 314 360 784 245 1408
Fragments arranged by size:
1408 784 552 360 314 295 276 275
269 245 231 183 144 84
NaeI GCC'GGC
Cuts at: 3601 3601
Size: 5420
NarI GG'CG_CC
Cuts at: 2851 3099 2851
Size: 248 5172
NcoI C'CATG_G
Cuts at: 974 974
Size: 5420
NdeI CA'TA_TG
Cuts at: 1150 1150
Size: 5420
NgoMIV G'CCGG_C
Cuts at: 3599 3599
Size: 5420
NspI r_CATG'y
Cuts at: 2085 2473 2973 4576 2085
Size: 388 500 1603 2929
Fragments arranged by size:
2929 1603 500 388
PciI A'CATG_T
Cuts at: 2081 4572 2081
Size: 2491 2929
PflMI CCAn_nnn'nTGG
Cuts at: 624 1227 624
Size: 603 4817
PfoI T'CCnGG_A
Cuts at: 1022 1430 1022
Size: 408 5012
PmeI GTTT'AAAC
Cuts at: 2419 2419
Size: 5420
PmlI CAC'GTG
Cuts at: 2019 2019
Size: 5420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 523 555 3866 3898 523
Size: 32 3311 32 2045
Fragments arranged by size:
3311 2045 32 32
PpuMI rG'GwC_Cy
Cuts at: 176 184 652 1106 1947 2293 176
Size: 8 468 454 841 346 3303
Fragments arranged by size:
3303 841 468 454 346 8
PspOMI G'GGCC_C
Cuts at: 339 339
Size: 5420
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 586 618 586
Size: 32 5388
PstI C_TGCA'G
Cuts at: 1388 2008 2185 2236 2467 3152 1388
Size: 620 177 51 231 685 3656
Fragments arranged by size:
3656 685 620 231 177 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5420
PvuII CAG'CTG
Cuts at: 222 306 858 1403 2025 2845 3205 222
Size: 84 552 545 622 820 360 2437
Fragments arranged by size:
2437 820 622 552 545 360 84
RsrII CG'GwC_CG
Cuts at: 3615 3615
Size: 5420
SacI G_AGCT'C
Cuts at: 198 280 1270 1935 2434 198
Size: 82 990 665 499 3184
Fragments arranged by size:
3184 990 665 499 82
SalI G'TCGA_C
Cuts at: 2457 2457
Size: 5420
SanDI GG'GwC_CC
Cuts at: 176 652 176
Size: 476 4944
SapI GCTCTTCn'nnn_
Cuts at: 3443 3653 3443
Size: 210 5210
SbfI CC_TGCA'GG
Cuts at: 2467 2467
Size: 5420
ScaI AGT'ACT
Cuts at: 4937 4937
Size: 5420
SexAI A'CCwGG_T
Cuts at: 1949 1949
Size: 5420
SfcI C'TryA_G
Cuts at: 33 803 1356 1384 2004 2181 2232 2463 3148
Size: 770 553 28 620 177 51 231 685
Cuts at: 3148 4116 4307 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 770 685 620 553 231 191
177 51 28
SfoI GGC'GCC
Cuts at: 2852 3100 2852
Size: 248 5172
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
SmaI CCC'GGG
Cuts at: 1756 2442 1756
Size: 686 4734
SmlI C'TyrA_G
Cuts at: 1423 2575 3490 4204 4466 1423
Size: 1152 915 714 262 2377
Fragments arranged by size:
2377 1152 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5420
SphI G_CATG'C
Cuts at: 2473 2973 2473
Size: 500 4920
StyI C'CwwG_G
Cuts at: 180 634 974 2554 2908 180
Size: 454 340 1580 354 2692
Fragments arranged by size:
2692 1580 454 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1102 2280 3003 3136 3595 3804 1102
Size: 1178 723 133 459 209 2718
Fragments arranged by size:
2718 1178 723 459 209 133
TatI w'GTAC_w
Cuts at: 1370 4935 1370
Size: 3565 1855
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 447 1270 1302 1750 1782 1824 1856 1882
Size: 32 823 32 448 32 42 32 26
Cuts at: 1882 1914 2003 2035 4937 4969 415
Size: 32 89 32 2902 32 866
Fragments arranged by size:
2902 866 823 448 89 42 32 32
32 32 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 3217 3217
Size: 5420
XbaI T'CTAG_A
Cuts at: 2451 2451
Size: 5420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 187 571 625 2915 187
Size: 384 54 2290 2692
Fragments arranged by size:
2692 2290 384 54
XmaI C'CCGG_G
Cuts at: 1754 2440 1754
Size: 686 4734
XmnI GAAnn'nnTTC
Cuts at: 1456 1456
Size: 5420
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AscI AvrII BbvCI
BcgI BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspXI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI