Restriction map for
pF1KB7296
(Circular) (Six-base) MAPSORT of: pF1KB7296.seq Check: 9787 from: 1 to: 7112
pF1KB7296 7112 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 4150 4150
Size: 7112
Acc65I G'GTAC_C
Cuts at: 3250 4128 3250
Size: 878 6234
AclI AA'CG_TT
Cuts at: 6952 6952
Size: 7112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 293 1721 1874 4937 5369 5722 6384 226
Size: 67 1428 153 3063 432 353 662 954
Fragments arranged by size:
3063 1428 954 662 432 353 153 67
AflIII A'CryG_T
Cuts at: 324 1459 1461 4464 6264 6545 324
Size: 1135 2 3003 1800 281 891
Fragments arranged by size:
3003 1800 1135 891 281 2
AlwNI CAG_nnn'CTG
Cuts at: 123 1846 1921 2065 2080 3340 3589 4417 5855
Size: 1723 75 144 15 1260 249 828 1438
Cuts at: 5855 123
Size: 1380
Fragments arranged by size:
1723 1438 1380 1260 828 249 144 75
15
ApaLI G'TGCA_C
Cuts at: 5950 5950
Size: 7112
ApoI r'AATT_y
Cuts at: 434 740 1435 1898 2054 2254 2637 2742 2804
Size: 306 695 463 156 200 383 105 62
Cuts at: 2804 2900 3118 3348 4116 6465 6721 434
Size: 96 218 230 768 2349 256 825
Fragments arranged by size:
2349 825 768 695 463 383 306 256
230 218 200 156 105 96 62
AseI AT'TA_AT
Cuts at: 20 259 656 1610 2873 20
Size: 239 397 954 1263 4259
Fragments arranged by size:
4259 1263 954 397 239
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7112
AvaI C'yCGr_G
Cuts at: 220 1004 4132 220
Size: 784 3128 3200
Fragments arranged by size:
3200 3128 784
AvrII C'CTAG_G
Cuts at: 459 3658 459
Size: 3199 3913
BamHI G'GATC_C
Cuts at: 3308 4137 3308
Size: 829 6283
BanI G'GyrC_C
Cuts at: 177 3250 4128 4542 4790 4825 177
Size: 3073 878 414 248 35 2464
Fragments arranged by size:
3073 2464 878 414 248 35
BanII G_rGCy'C
Cuts at: 794 3442 4126 5156 794
Size: 2648 684 1030 2750
Fragments arranged by size:
2750 2648 1030 684
BbeI G_GCGC'C
Cuts at: 4546 4794 4546
Size: 248 6864
BbsI GAAGACnn'nnnn_
Cuts at: 965 1217 1308 2549 3582 965
Size: 252 91 1241 1033 4495
Fragments arranged by size:
4495 1241 1033 252 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7078
BciVI GTATCCnnnnn_n'
Cuts at: 5010 6066 7112 5010
Size: 1056 1046 5010
Fragments arranged by size:
5010 1056 1046
BclI T'GATC_A
Cuts at: 4632 4632
Size: 7112
BfrBI ATG'CAT
Cuts at: 2230 2230
Size: 7112
BglI GCCn_nnn'nGGC
Cuts at: 112 162 112
Size: 50 7062
BglII A'GATC_T
Cuts at: 2487 3499 4627 6664 2487
Size: 1012 1128 2037 2935
Fragments arranged by size:
2935 2037 1128 1012
BlpI GC'TnA_GC
Cuts at: 293 4224 293
Size: 3931 3181
Bme1580I G_kGCm'C
Cuts at: 2417 4737 4830 5954 2417
Size: 2320 93 1124 3575
Fragments arranged by size:
3575 2320 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 418 895 2202 4324 4405 4740 6884 418
Size: 477 1307 2122 81 335 2144 646
Fragments arranged by size:
2144 2122 1307 646 477 335 81
BmtI G_CTAG'C
Cuts at: 1559 1559
Size: 7112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1866 2396 5479 1866
Size: 530 3083 3499
Fragments arranged by size:
3499 3083 530
Bpu10I CC'TnA_GC
Cuts at: 2066 3170 2066
Size: 1104 6008
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2136 2743 3435 3771 4288 5167 5881 6179
Size: 133 607 692 336 517 879 714 298
Cuts at: 6179 2003
Size: 2936
Fragments arranged by size:
2936 879 714 692 607 517 336 298
133
BsaI GGTCTCn'nnnn_
Cuts at: 6816 6816
Size: 7112
BsaAI yAC'GTr
Cuts at: 1462 1949 4465 1462
Size: 487 2516 4109
Fragments arranged by size:
4109 2516 487
BsaBI GATnn'nnATC
Cuts at: 6 2231 3428 4626 6663 6
Size: 2225 1197 1198 2037 455
Fragments arranged by size:
2225 2037 1198 1197 455
BsaHI Gr'CG_yC
Cuts at: 4543 4791 5493 6989 4543
Size: 248 702 1496 4666
Fragments arranged by size:
4666 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1745 3707 4301 4822 5500 5911 6058 6332 1745
Size: 1962 594 521 678 411 147 274 2525
Fragments arranged by size:
2525 1962 678 594 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3788 3818 3867 3885 3897 3915 3788
Size: 30 49 18 12 18 6985
Fragments arranged by size:
6985 49 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1813 3548 3875 3878 3893 1813
Size: 1735 327 3 15 5032
Fragments arranged by size:
5032 1735 327 15 3
BseYI C'CCAG_C
Cuts at: 3594 3645 3970 4537 5295 5960 6368 3594
Size: 51 325 567 758 665 408 4338
Fragments arranged by size:
4338 758 665 567 408 325 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 3326 2141
Size: 1185 5927
BsiEI CG_ry'CG
Cuts at: 104 4654 4700 5930 104
Size: 4550 46 1230 1286
Fragments arranged by size:
4550 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2204 4049 4126 4904 5094 5954 2204
Size: 1845 77 778 190 860 3362
Fragments arranged by size:
3362 1845 860 778 190 77
BsmI GAATG_Cn'
Cuts at: 4098 4098
Size: 7112
Bsp1286I G_dGCh'C
Cuts at: 794 2204 2417 3442 4049 4126 4737 4830 4904
Size: 1410 213 1025 607 77 611 93 74
Cuts at: 4904 5094 5156 5954 794
Size: 190 62 798 1952
Fragments arranged by size:
1952 1410 1025 798 611 607 213 190
93 77 74 62
BspHI T'CATG_A
Cuts at: 1029 2820 5544 1029
Size: 1791 2724 2597
Fragments arranged by size:
2724 2597 1791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2441 4162 4376 4678 5059 2441
Size: 1721 214 302 381 4494
Fragments arranged by size:
4494 1721 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5404 5458 6942 7107 50
Size: 5354 54 1484 165 55
Fragments arranged by size:
5354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 608 1566 2288 3462 3849 3965 5024 608
Size: 958 722 1174 387 116 1059 2696
Fragments arranged by size:
2696 1174 1059 958 722 387 116
BsrFI r'CCGG_y
Cuts at: 5110 5291 5110
Size: 181 6931
BsrGI T'GTAC_A
Cuts at: 2502 2502
Size: 7112
BssSI C'ACGA_G
Cuts at: 3755 5383 6091 3755
Size: 1628 708 4776
Fragments arranged by size:
4776 1628 708
BstAPI GCAn_nnn'nTGC
Cuts at: 123 607 4489 123
Size: 484 3882 2746
Fragments arranged by size:
3882 2746 484
BstBI TT'CG_AA
Cuts at: 5473 5473
Size: 7112
BstYI r'GATC_y
Cuts at: 1 1965 2002 2487 3017 3308 3499 4137 4604
Size: 1964 37 485 530 291 191 638 467
Cuts at: 4604 4627 4962 5208 5514 5526 5612 6664 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1964 1052 638 530 485 467 449 335
306 291 246 191 86 37 23 12
Bsu36I CC'TnA_GG
Cuts at: 693 2942 693
Size: 2249 4863
BtgI C'CryG_G
Cuts at: 3489 3837 3489
Size: 348 6764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5062 5243 5406 5062
Size: 181 163 6768
Fragments arranged by size:
6768 181 163
BtsI GCAGTG_nn'
Cuts at: 3420 4355 3420
Size: 935 6177
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4067 4102 4067
Size: 35 7077
DraI TTT'AAA
Cuts at: 533 1226 1661 4111 533
Size: 693 435 2450 3534
Fragments arranged by size:
3534 2450 693 435
DraIII CAC_nnn'GTG
Cuts at: 2606 2884 2606
Size: 278 6834
DrdI GACnn_nn'nnGTC
Cuts at: 4818 6162 4818
Size: 1344 5768
EaeI y'GGCC_r
Cuts at: 4697 4871 5262 5289 6634 4697
Size: 174 391 27 1345 5175
Fragments arranged by size:
5175 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4697 4697
Size: 7112
EarI CTCTTCn'nnn_
Cuts at: 140 245 674 1697 1784 1850 1868 5135 5345
Size: 105 429 1023 87 66 18 3267 210
Cuts at: 5345 140
Size: 1907
Fragments arranged by size:
3267 1907 1023 429 210 105 87 66
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3365 5320 6062 6208 6922 3365
Size: 1955 742 146 714 3555
Fragments arranged by size:
3555 1955 742 714 146
EcoICRI GAG'CTC
Cuts at: 4124 4124
Size: 7112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 293 1721 1874 4937 5369 5722 6384 226
Size: 67 1428 153 3063 432 353 662 954
Fragments arranged by size:
3063 1428 954 662 432 353 153 67
EcoO109I rG'GnC_Cy
Cuts at: 456 2946 4251 456
Size: 2490 1305 3317
Fragments arranged by size:
3317 2490 1305
EcoRI G'AATT_C
Cuts at: 1898 2742 4116 1898
Size: 844 1374 4894
Fragments arranged by size:
4894 1374 844
EcoRV GAT'ATC
Cuts at: 1733 3066 1733
Size: 1333 5779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 428 1471 3521 3973 5131 428
Size: 1043 2050 452 1158 2409
Fragments arranged by size:
2409 2050 1158 1043 452
FspI TGC'GCA
Cuts at: 4893 4893
Size: 7112
HaeII r_GCGC'y
Cuts at: 4546 4794 6024 6795 4546
Size: 248 1230 771 4863
Fragments arranged by size:
4863 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 2820 2852 3697 3729 3769 3801 4954
Size: 32 2424 32 845 32 40 32 1153
Cuts at: 4954 4984 4986 5016 364
Size: 30 2 30 2460
Fragments arranged by size:
2460 2424 1153 845 40 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3368 4151 3368
Size: 783 6329
HpaI GTT'AAC
Cuts at: 3368 3368
Size: 7112
KasI G'GCGC_C
Cuts at: 4542 4790 4542
Size: 248 6864
KpnI G_GTAC'C
Cuts at: 3254 4132 3254
Size: 878 6234
MfeI C'AATT_G
Cuts at: 350 1713 350
Size: 1363 5749
MluI A'CGCG_T
Cuts at: 6545 6545
Size: 7112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2502 3831 4067 5871 6055 2502
Size: 1329 236 1804 184 3559
Fragments arranged by size:
3559 1804 1329 236 184
MscI TGG'CCA
Cuts at: 4873 4873
Size: 7112
MslI CAynn'nnrTG
Cuts at: 641 2210 3842 4070 5101 5228 641
Size: 1569 1632 228 1031 127 2525
Fragments arranged by size:
2525 1632 1569 1031 228 127
MspA1I CmG'CkG
Cuts at: 123 1699 4223 4537 4897 5681 5926 123
Size: 1576 2524 314 360 784 245 1309
Fragments arranged by size:
2524 1576 1309 784 360 314 245
NaeI GCC'GGC
Cuts at: 5293 5293
Size: 7112
NarI GG'CG_CC
Cuts at: 4543 4791 4543
Size: 248 6864
NcoI C'CATG_G
Cuts at: 3489 3837 3489
Size: 348 6764
NdeI CA'TA_TG
Cuts at: 990 1955 990
Size: 965 6147
NgoMIV G'CCGG_C
Cuts at: 5291 5291
Size: 7112
NheI G'CTAG_C
Cuts at: 1555 1555
Size: 7112
NsiI A_TGCA'T
Cuts at: 2232 2232
Size: 7112
NspI r_CATG'y
Cuts at: 1322 1645 1884 4165 4665 6268 1322
Size: 323 239 2281 500 1603 2166
Fragments arranged by size:
2281 2166 1603 500 323 239
PciI A'CATG_T
Cuts at: 6264 6264
Size: 7112
PmeI GTTT'AAAC
Cuts at: 4111 4111
Size: 7112
PmlI CAC'GTG
Cuts at: 1462 1462
Size: 7112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5558 5590 5558
Size: 32 7080
PpuMI rG'GwC_Cy
Cuts at: 456 2946 456
Size: 2490 4622
PsiI TTA'TAA
Cuts at: 467 775 1441 2038 2974 467
Size: 308 666 597 936 4605
Fragments arranged by size:
4605 936 666 597 308
PstI C_TGCA'G
Cuts at: 525 3278 4159 4844 525
Size: 2753 881 685 2793
Fragments arranged by size:
2793 2753 881 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7112
PvuII CAG'CTG
Cuts at: 123 1699 4537 4897 123
Size: 1576 2838 360 2338
Fragments arranged by size:
2838 2338 1576 360
RsrII CG'GwC_CG
Cuts at: 5307 5307
Size: 7112
SacI G_AGCT'C
Cuts at: 4126 4126
Size: 7112
SalI G'TCGA_C
Cuts at: 4149 4149
Size: 7112
SapI GCTCTTCn'nnn_
Cuts at: 245 674 5135 5345 245
Size: 429 4461 210 2012
Fragments arranged by size:
4461 2012 429 210
SbfI CC_TGCA'GG
Cuts at: 4159 4159
Size: 7112
ScaI AGT'ACT
Cuts at: 6629 6629
Size: 7112
SexAI A'CCwGG_T
Cuts at: 479 1020 2349 3246 479
Size: 541 1329 897 4345
Fragments arranged by size:
4345 1329 897 541
SfcI C'TryA_G
Cuts at: 33 521 1783 2093 3274 4155 4840 5808 5999
Size: 488 1262 310 1181 881 685 968 191
Cuts at: 5999 33
Size: 1146
Fragments arranged by size:
1262 1181 1146 968 881 685 488 310
191
SfoI GGC'GCC
Cuts at: 4544 4792 4544
Size: 248 6864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7112
SmaI CCC'GGG
Cuts at: 4134 4134
Size: 7112
SmlI C'TyrA_G
Cuts at: 220 1004 2018 2115 2722 3450 3786 4267 5182
Size: 784 1014 97 607 728 336 481 915
Cuts at: 5182 5896 6158 220
Size: 714 262 1174
Fragments arranged by size:
1174 1014 915 784 728 714 607 481
336 262 97
SnaBI TAC'GTA
Cuts at: 1949 1949
Size: 7112
SpeI A'CTAG_T
Cuts at: 67 939 67
Size: 872 6240
SphI G_CATG'C
Cuts at: 1645 4165 4665 1645
Size: 2520 500 4092
Fragments arranged by size:
4092 2520 500
SspI AAT'ATT
Cuts at: 1392 1863 2361 2851 1392
Size: 471 498 490 5653
Fragments arranged by size:
5653 498 490 471
StyI C'CwwG_G
Cuts at: 459 1889 3286 3489 3658 3837 4246 4600 459
Size: 1430 1397 203 169 179 409 354 2971
Fragments arranged by size:
2971 1430 1397 409 354 203 179 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2462 4070 4695 4828 5287 5496 2462
Size: 1608 625 133 459 209 4078
Fragments arranged by size:
4078 1608 625 459 209 133
TatI w'GTAC_w
Cuts at: 1750 2502 3331 6627 1750
Size: 752 829 3296 2235
Fragments arranged by size:
3296 2235 829 752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 161 193 3788 3820 6629 6661 161
Size: 32 3595 32 2809 32 612
Fragments arranged by size:
3595 2809 612 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2351 4909 2351
Size: 2558 4554
XbaI T'CTAG_A
Cuts at: 4143 4143
Size: 7112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 356 1134 4607 356
Size: 778 3473 2861
Fragments arranged by size:
3473 2861 778
XhoI C'TCGA_G
Cuts at: 220 1004 220
Size: 784 6328
XmaI C'CCGG_G
Cuts at: 4132 4132
Size: 7112
XmnI GAAnn'nnTTC
Cuts at: 306 326 434 1109 306
Size: 20 108 675 6309
Fragments arranged by size:
6309 675 108 20
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PciI PmeI PmlI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI BaeI BbvCI BmgBI BpmI BsiWI
BsmBI BspEI BssHII BstEII BstXI BstZ17I ClaI EcoNI
FseI FspAI HindIII NotI NruI PacI PasI PflMI
PfoI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI StuI SwaI ZraI