Restriction map for
pF1KB7298
(Circular) (Six-base) MAPSORT of: pF1KB7298.seq Check: 616 from: 1 to: 6164
pF1KB7298 6164 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 236 3202 236
Size: 2966 3198
Acc65I G'GTAC_C
Cuts at: 3180 3180
Size: 6164
AclI AA'CG_TT
Cuts at: 6004 6004
Size: 6164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2990 3989 4421 4774 5436 1135
Size: 1855 999 432 353 662 1863
Fragments arranged by size:
1863 1855 999 662 432 353
AflIII A'CryG_T
Cuts at: 695 1439 3149 3516 5316 5597 695
Size: 744 1710 367 1800 281 1262
Fragments arranged by size:
1800 1710 1262 744 367 281
AleI CACnn'nnGTG
Cuts at: 964 1423 964
Size: 459 5705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 211 211
Size: 6164
AlwNI CAG_nnn'CTG
Cuts at: 354 1158 1796 2076 3013 3064 3469 4907 354
Size: 804 638 280 937 51 405 1438 1611
Fragments arranged by size:
1611 1438 937 804 638 405 280 51
ApaI G_GGCC'C
Cuts at: 1809 1809
Size: 6164
ApaLI G'TGCA_C
Cuts at: 1105 1609 5002 1105
Size: 504 3393 2267
Fragments arranged by size:
3393 2267 504
ApoI r'AATT_y
Cuts at: 598 3168 5517 5773 598
Size: 2570 2349 256 989
Fragments arranged by size:
2570 2349 989 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6164
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
AvaI C'yCGr_G
Cuts at: 721 858 2150 2369 3184 721
Size: 137 1292 219 815 3701
Fragments arranged by size:
3701 1292 815 219 137
BamHI G'GATC_C
Cuts at: 3189 3189
Size: 6164
BanI G'GyrC_C
Cuts at: 431 1259 1335 1495 2679 3180 3594 3842 3877
Size: 828 76 160 1184 501 414 248 35
Cuts at: 3877 431
Size: 2718
Fragments arranged by size:
2718 1184 828 501 414 248 160 76
35
BanII G_rGCy'C
Cuts at: 263 1809 2182 2377 3178 4208 263
Size: 1546 373 195 801 1030 2219
Fragments arranged by size:
2219 1546 1030 801 373 195
BbeI G_GCGC'C
Cuts at: 1263 1499 2683 3598 3846 1263
Size: 236 1184 915 248 3581
Fragments arranged by size:
3581 1184 915 248 236
BbsI GAAGACnn'nnnn_
Cuts at: 301 621 1123 301
Size: 320 502 5342
Fragments arranged by size:
5342 502 320
BbvCI CC'TCA_GC
Cuts at: 2096 2197 2353 2531 2621 2726 2902 2096
Size: 101 156 178 90 105 176 5358
Fragments arranged by size:
5358 178 176 156 105 101 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1030 1064 1732 1766 90
Size: 34 906 34 668 34 4488
Fragments arranged by size:
4488 906 668 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2949 4062 5118 6164 2949
Size: 1113 1056 1046 2949
Fragments arranged by size:
2949 1113 1056 1046
BclI T'GATC_A
Cuts at: 3684 3684
Size: 6164
BglI GCCn_nnn'nGGC
Cuts at: 656 665 674 1879 2137 2331 2393 2722 656
Size: 9 9 1205 258 194 62 329 4098
Fragments arranged by size:
4098 1205 329 258 194 62 9 9
BglII A'GATC_T
Cuts at: 1466 2765 3679 5716 1466
Size: 1299 914 2037 1914
Fragments arranged by size:
2037 1914 1299 914
BlpI GC'TnA_GC
Cuts at: 420 2077 2797 3276 420
Size: 1657 720 479 3308
Fragments arranged by size:
3308 1657 720 479
Bme1580I G_kGCm'C
Cuts at: 217 722 1109 1340 1613 1809 3789 3882 5006
Size: 505 387 231 273 196 1980 93 1124
Cuts at: 5006 217
Size: 1375
Fragments arranged by size:
1980 1375 1124 505 387 273 231 196
93
BmrI ACTGGGnnnn_n'
Cuts at: 966 2560 3376 3457 3792 5936 966
Size: 1594 816 81 335 2144 1194
Fragments arranged by size:
2144 1594 1194 816 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1185 4531 1185
Size: 3346 2818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1180 1351 2935 3068 388
Size: 792 171 1584 133 3484
Fragments arranged by size:
3484 1584 792 171 133
Bpu10I CC'TnA_GC
Cuts at: 1979 2096 2197 2353 2531 2621 2726 2902 1979
Size: 117 101 156 178 90 105 176 5241
Fragments arranged by size:
5241 178 176 156 117 105 101 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1576 1615 1930 3048 3141 3340 4219 4933 5231
Size: 39 315 1118 93 199 879 714 298
Cuts at: 5231 1576
Size: 2509
Fragments arranged by size:
2509 1118 879 714 315 298 199 93
39
BsaI GGTCTCn'nnnn_
Cuts at: 322 755 3024 5868 322
Size: 433 2269 2844 618
Fragments arranged by size:
2844 2269 618 433
BsaAI yAC'GTr
Cuts at: 1664 3517 1664
Size: 1853 4311
BsaBI GATnn'nnATC
Cuts at: 6 451 3678 5715 6
Size: 445 3227 2037 455
Fragments arranged by size:
3227 2037 455 445
BsaHI Gr'CG_yC
Cuts at: 1260 1496 1688 2680 3595 3843 4545 6041 1260
Size: 236 192 992 915 248 702 1496 1383
Fragments arranged by size:
1496 1383 992 915 702 248 236 192
BsaWI w'CCGG_w
Cuts at: 2568 3353 3874 4552 4963 5110 5384 2568
Size: 785 521 678 411 147 274 3348
Fragments arranged by size:
3348 785 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 763 793 1984 2014 2321 2351 2470
Size: 30 180 30 1191 30 307 30 119
Cuts at: 2470 2500 2933 2963 553
Size: 30 433 30 3754
Fragments arranged by size:
3754 1191 433 307 180 119 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 751 754 986 2244 2247 2413 2418 2743 751
Size: 3 232 1258 3 166 5 325 4172
Fragments arranged by size:
4172 1258 325 232 166 5 3 3
BseYI C'CCAG_C
Cuts at: 2228 2358 2701 2841 2859 2944 2982 3589 4347
Size: 130 343 140 18 85 38 607 758
Cuts at: 4347 5012 5420 2228
Size: 665 408 2972
Fragments arranged by size:
2972 758 665 607 408 343 140 130
85 38 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1197 1464 1801 340
Size: 857 267 337 4703
Fragments arranged by size:
4703 857 337 267
BsiEI CG_ry'CG
Cuts at: 104 276 1627 3706 3752 4982 104
Size: 172 1351 2079 46 1230 1286
Fragments arranged by size:
2079 1351 1286 1230 172 46
BsiHKAI G_wGCw'C
Cuts at: 263 386 1109 1193 1613 1919 2512 3178 3956
Size: 123 723 84 420 306 593 666 778
Cuts at: 3956 4146 5006 263
Size: 190 860 1421
Fragments arranged by size:
1421 860 778 723 666 593 420 306
190 123 84
BsmBI CGTCTCn'nnnn_
Cuts at: 783 2779 783
Size: 1996 4168
Bsp1286I G_dGCh'C
Cuts at: 217 263 386 722 1109 1193 1340 1613 1809
Size: 46 123 336 387 84 147 273 196
Cuts at: 1809 1919 2182 2377 2512 3178 3789 3882 3956
Size: 110 263 195 135 666 611 93 74
Cuts at: 3956 4146 4208 5006 217
Size: 190 62 798 1375
Fragments arranged by size:
1375 798 666 611 387 336 273 263
196 195 190 147 135 123 110 93
84 74 62 46
BspEI T'CCGG_A
Cuts at: 2568 2568
Size: 6164
BspHI T'CATG_A
Cuts at: 116 4596 116
Size: 4480 1684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 307 1076 2177 3214 3428 3730 4111 307
Size: 769 1101 1037 214 302 381 2360
Fragments arranged by size:
2360 1101 1037 769 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1164 1562 1629 2237 2672 4456 4510 5994
Size: 1114 398 67 608 435 1784 54 1484
Cuts at: 5994 6159 50
Size: 165 55
Fragments arranged by size:
1784 1484 1114 608 435 398 165 67
55 54
BsrDI GCAATG_nn'
Cuts at: 1733 4076 1733
Size: 2343 3821
BsrFI r'CCGG_y
Cuts at: 111 646 2750 4162 4343 111
Size: 535 2104 1412 181 1932
Fragments arranged by size:
2104 1932 1412 535 181
BssSI C'ACGA_G
Cuts at: 4435 5143 4435
Size: 708 5456
BstAPI GCAn_nnn'nTGC
Cuts at: 354 1617 1641 1796 3541 354
Size: 1263 24 155 1745 2977
Fragments arranged by size:
2977 1745 1263 155 24
BstBI TT'CG_AA
Cuts at: 4525 4525
Size: 6164
BstEII G'GTnAC_C
Cuts at: 831 831
Size: 6164
BstYI r'GATC_y
Cuts at: 1 1466 1757 2765 3189 3656 3679 4014 4260
Size: 1465 291 1008 424 467 23 335 246
Cuts at: 4260 4566 4578 4664 5716 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1465 1052 1008 467 449 424 335 306
291 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2104 2104
Size: 6164
BtgI C'CryG_G
Cuts at: 546 740 914 1621 1863 2999 546
Size: 194 174 707 242 1136 3711
Fragments arranged by size:
3711 1136 707 242 194 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 144 894 1371 4114 4295 4458 144
Size: 750 477 2743 181 163 1850
Fragments arranged by size:
2743 1850 750 477 181 163
BtsI GCAGTG_nn'
Cuts at: 1185 2976 3407 1185
Size: 1791 431 3942
Fragments arranged by size:
3942 1791 431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 153 188 153
Size: 35 6129
DraI TTT'AAA
Cuts at: 3163 3163
Size: 6164
DraIII CAC_nnn'GTG
Cuts at: 1512 1512
Size: 6164
DrdI GACnn_nn'nnGTC
Cuts at: 3870 5214 3870
Size: 1344 4820
EaeI y'GGCC_r
Cuts at: 200 648 657 978 1022 1624 1937 2469 2714
Size: 448 9 321 44 602 313 532 245
Cuts at: 2714 3749 3923 4314 4341 5686 200
Size: 1035 174 391 27 1345 678
Fragments arranged by size:
1345 1035 678 602 532 448 391 321
313 245 174 44 27 9
EagI C'GGCC_G
Cuts at: 1624 3749 1624
Size: 2125 4039
EarI CTCTTCn'nnn_
Cuts at: 931 4187 4397 931
Size: 3256 210 2698
Fragments arranged by size:
3256 2698 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4372 5114 5260 5974 4372
Size: 742 146 714 4562
Fragments arranged by size:
4562 742 714 146
EcoICRI GAG'CTC
Cuts at: 261 3176 261
Size: 2915 3249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1135 1180 1351 2935 2990 3068 3989 4421
Size: 747 45 171 1584 55 78 921 432
Cuts at: 4421 4774 5436 388
Size: 353 662 1116
Fragments arranged by size:
1584 1116 921 747 662 432 353 171
78 55 45
EcoNI CCTnn'n_nnAGG
Cuts at: 1948 2173 2830 1948
Size: 225 657 5282
Fragments arranged by size:
5282 657 225
EcoO109I rG'GnC_Cy
Cuts at: 1587 1806 1956 2100 2154 2324 2625 2666 2845
Size: 219 150 144 54 170 301 41 179
Cuts at: 2845 3076 3303 1587
Size: 231 227 4448
Fragments arranged by size:
4448 301 231 227 219 179 170 150
144 54 41
EcoRI G'AATT_C
Cuts at: 3168 3168
Size: 6164
EcoRV GAT'ATC
Cuts at: 504 939 1974 504
Size: 435 1035 4694
Fragments arranged by size:
4694 1035 435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4183 4183
Size: 6164
FspI TGC'GCA
Cuts at: 3945 3945
Size: 6164
HaeII r_GCGC'y
Cuts at: 1263 1382 1499 2683 3598 3846 5076 5847 1263
Size: 119 117 1184 915 248 1230 771 1580
Fragments arranged by size:
1580 1230 1184 915 771 248 119 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1958 1990 2097 2129 2321 2353 2757 2789 4006
Size: 32 107 32 192 32 404 32 1217
Cuts at: 4006 4036 4038 4068 1958
Size: 30 2 30 4054
Fragments arranged by size:
4054 1217 404 192 107 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1674 3203 1674
Size: 1529 4635
KasI G'GCGC_C
Cuts at: 1259 1495 2679 3594 3842 1259
Size: 236 1184 915 248 3581
Fragments arranged by size:
3581 1184 915 248 236
KpnI G_GTAC'C
Cuts at: 3184 3184
Size: 6164
MluI A'CGCG_T
Cuts at: 5597 5597
Size: 6164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 269 4923 5107 269
Size: 4654 184 1326
Fragments arranged by size:
4654 1326 184
MscI TGG'CCA
Cuts at: 202 1024 1939 2471 2716 3925 202
Size: 822 915 532 245 1209 2441
Fragments arranged by size:
2441 1209 915 822 532 245
MslI CAynn'nnrTG
Cuts at: 913 964 1090 1423 1606 1738 1889 4153 4280
Size: 51 126 333 183 132 151 2264 127
Cuts at: 4280 913
Size: 2797
Fragments arranged by size:
2797 2264 333 183 151 132 127 126
51
MspA1I CmG'CkG
Cuts at: 305 548 1287 1623 1679 1767 2076 2620 2901
Size: 243 739 336 56 88 309 544 281
Cuts at: 2901 3275 3589 3949 4733 4978 305
Size: 374 314 360 784 245 1491
Fragments arranged by size:
1491 784 739 544 374 360 336 314
309 281 245 243 88 56
NaeI GCC'GGC
Cuts at: 2752 4345 2752
Size: 1593 4571
NarI GG'CG_CC
Cuts at: 1260 1496 2680 3595 3843 1260
Size: 236 1184 915 248 3581
Fragments arranged by size:
3581 1184 915 248 236
NcoI C'CATG_G
Cuts at: 2999 2999
Size: 6164
NgoMIV G'CCGG_C
Cuts at: 2750 4343 2750
Size: 1593 4571
NotI GC'GGCC_GC
Cuts at: 1624 1624
Size: 6164
NruI TCG'CGA
Cuts at: 888 888
Size: 6164
NspI r_CATG'y
Cuts at: 1551 1644 3217 3717 5320 1551
Size: 93 1573 500 1603 2395
Fragments arranged by size:
2395 1603 1573 500 93
PciI A'CATG_T
Cuts at: 5316 5316
Size: 6164
PflMI CCAn_nnn'nTGG
Cuts at: 529 1020 1233 529
Size: 491 213 5460
Fragments arranged by size:
5460 491 213
PfoI T'CCnGG_A
Cuts at: 149 437 2951 3020 149
Size: 288 2514 69 3293
Fragments arranged by size:
3293 2514 288 69
PmeI GTTT'AAAC
Cuts at: 3163 3163
Size: 6164
PmlI CAC'GTG
Cuts at: 1664 1664
Size: 6164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 4610
Size: 32 6132
PpuMI rG'GwC_Cy
Cuts at: 1587 1956 2100 2154 2845 3076 1587
Size: 369 144 54 691 231 4675
Fragments arranged by size:
4675 691 369 231 144 54
PshAI GACnn'nnGTC
Cuts at: 2576 2576
Size: 6164
PspOMI G'GGCC_C
Cuts at: 1805 1805
Size: 6164
PstI C_TGCA'G
Cuts at: 304 350 1154 1766 1880 2072 2834 3211 3896
Size: 46 804 612 114 192 762 377 685
Cuts at: 3896 304
Size: 2572
Fragments arranged by size:
2572 804 762 685 612 377 192 114
46
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6164
PvuII CAG'CTG
Cuts at: 305 1767 2076 2620 2901 3589 3949 305
Size: 1462 309 544 281 688 360 2520
Fragments arranged by size:
2520 1462 688 544 360 309 281
RsrII CG'GwC_CG
Cuts at: 4359 4359
Size: 6164
SacI G_AGCT'C
Cuts at: 263 3178 263
Size: 2915 3249
SacII CC_GC'GG
Cuts at: 549 1624 549
Size: 1075 5089
SalI G'TCGA_C
Cuts at: 3201 3201
Size: 6164
SanDI GG'GwC_CC
Cuts at: 2845 3076 2845
Size: 231 5933
SapI GCTCTTCn'nnn_
Cuts at: 931 4187 4397 931
Size: 3256 210 2698
Fragments arranged by size:
3256 2698 210
SbfI CC_TGCA'GG
Cuts at: 1880 3211 1880
Size: 1331 4833
ScaI AGT'ACT
Cuts at: 5681 5681
Size: 6164
SexAI A'CCwGG_T
Cuts at: 1027 1027
Size: 6164
SfcI C'TryA_G
Cuts at: 33 300 346 771 1150 1762 1876 2068 2687
Size: 267 46 425 379 612 114 192 619
Cuts at: 2687 2830 3207 3892 4860 5051 33
Size: 143 377 685 968 191 1146
Fragments arranged by size:
1146 968 685 619 612 425 379 377
267 192 191 143 114 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 656 665 2331 656
Size: 9 1666 4489
Fragments arranged by size:
4489 1666 9
SfoI GGC'GCC
Cuts at: 1261 1497 2681 3596 3844 1261
Size: 236 1184 915 248 3581
Fragments arranged by size:
3581 1184 915 248 236
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
SmaI CCC'GGG
Cuts at: 2152 3186 2152
Size: 1034 5130
SmlI C'TyrA_G
Cuts at: 1591 1630 1909 3027 3120 3319 4234 4948 5210
Size: 39 279 1118 93 199 915 714 262
Cuts at: 5210 1591
Size: 2545
Fragments arranged by size:
2545 1118 915 714 279 262 199 93
39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6164
SphI G_CATG'C
Cuts at: 1644 3217 3717 1644
Size: 1573 500 4091
Fragments arranged by size:
4091 1573 500
StuI AGG'CCT
Cuts at: 2652 3097 2652
Size: 445 5719
StyI C'CwwG_G
Cuts at: 537 573 782 2662 2999 3298 3652 537
Size: 36 209 1880 337 299 354 3049
Fragments arranged by size:
3049 1880 354 337 299 209 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 593 1197 2873 2958 3747 3880 4339 4548 593
Size: 604 1676 85 789 133 459 209 2209
Fragments arranged by size:
2209 1676 789 604 459 209 133 85
TatI w'GTAC_w
Cuts at: 725 1397 1550 5679 725
Size: 672 153 4129 1210
Fragments arranged by size:
4129 1210 672 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 553 585 2470 2502 2933 2965 5681 5713 553
Size: 32 1885 32 431 32 2716 32 1004
Fragments arranged by size:
2716 1885 1004 431 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3961 3961
Size: 6164
XbaI T'CTAG_A
Cuts at: 3195 3195
Size: 6164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2709 3659 2709
Size: 950 5214
XmaI C'CCGG_G
Cuts at: 2150 3184 2150
Size: 1034 5130
XmnI GAAnn'nnTTC
Cuts at: 1468 1468
Size: 6164
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NruI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstXI BstZ17I ClaI FseI FspAI HindIII HpaI
MfeI NdeI NheI NsiI PacI PasI PsiI PspXI
PsrI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI