Restriction map for
pF1KB7301
(Circular) (Six-base) MAPSORT of: pF1KB7301.seq Check: 8702 from: 1 to: 5666
pF1KB7301 5666 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AatII G_ACGT'C
Cuts at: 1363 1363
Size: 5666
AccI GT'mk_AC
Cuts at: 1421 2704 1421
Size: 1283 4383
Acc65I G'GTAC_C
Cuts at: 2682 2682
Size: 5666
AclI AA'CG_TT
Cuts at: 5506 5506
Size: 5666
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 887 1067 1250 1355 1420 2087 2147 3491 3923
Size: 180 183 105 65 667 60 1344 432
Cuts at: 3923 4276 4938 887
Size: 353 662 1615
Fragments arranged by size:
1615 1344 667 662 432 353 183 180
105 65 60
AflIII A'CryG_T
Cuts at: 1147 3018 4818 5099 1147
Size: 1871 1800 281 1714
Fragments arranged by size:
1871 1800 1714 281
AhdI GACnn_n'nnGTC
Cuts at: 1359 1359
Size: 5666
AlwNI CAG_nnn'CTG
Cuts at: 337 2307 2971 4409 337
Size: 1970 664 1438 1594
Fragments arranged by size:
1970 1594 1438 664
ApaLI G'TGCA_C
Cuts at: 4504 4504
Size: 5666
ApoI r'AATT_y
Cuts at: 541 586 1270 1451 1477 1569 2670 5019 5275
Size: 45 684 181 26 92 1101 2349 256
Cuts at: 5275 541
Size: 932
Fragments arranged by size:
2349 1101 932 684 256 181 92 45
26
AseI AT'TA_AT
Cuts at: 20 2350 20
Size: 2330 3336
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5666
AvaI C'yCGr_G
Cuts at: 165 2686 165
Size: 2521 3145
BamHI G'GATC_C
Cuts at: 2691 2691
Size: 5666
BanI G'GyrC_C
Cuts at: 278 679 2682 3096 3344 3379 278
Size: 401 2003 414 248 35 2565
Fragments arranged by size:
2565 2003 414 401 248 35
BanII G_rGCy'C
Cuts at: 2680 3710 2680
Size: 1030 4636
BbeI G_GCGC'C
Cuts at: 282 3100 3348 282
Size: 2818 248 2600
Fragments arranged by size:
2818 2600 248
BciVI GTATCCnnnnn_n'
Cuts at: 3564 4620 5666 3564
Size: 1056 1046 3564
Fragments arranged by size:
3564 1056 1046
BclI T'GATC_A
Cuts at: 1650 3186 1650
Size: 1536 4130
BglII A'GATC_T
Cuts at: 1503 3181 5218 1503
Size: 1678 2037 1951
Fragments arranged by size:
2037 1951 1678
BlpI GC'TnA_GC
Cuts at: 2778 2778
Size: 5666
Bme1580I G_kGCm'C
Cuts at: 3291 3384 4508 3291
Size: 93 1124 4449
Fragments arranged by size:
4449 1124 93
BmgBI CAC'GTC
Cuts at: 1150 1150
Size: 5666
BmrI ACTGGGnnnn_n'
Cuts at: 1185 2878 2959 3294 5438 1185
Size: 1693 81 335 2144 1413
Fragments arranged by size:
2144 1693 1413 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4033 4033
Size: 5666
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 2174 844
Size: 1330 4336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2497 2842 3721 4435 4733 2497
Size: 345 879 714 298 3430
Fragments arranged by size:
3430 879 714 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 5370 5370
Size: 5666
BsaAI yAC'GTr
Cuts at: 3019 3019
Size: 5666
BsaBI GATnn'nnATC
Cuts at: 6 344 3180 5217 6
Size: 338 2836 2037 455
Fragments arranged by size:
2836 2037 455 338
BsaHI Gr'CG_yC
Cuts at: 279 1360 3097 3345 4047 5543 279
Size: 1081 1737 248 702 1496 402
Fragments arranged by size:
1737 1496 1081 702 402 248
BsaWI w'CCGG_w
Cuts at: 1434 2855 3376 4054 4465 4612 4886 1434
Size: 1421 521 678 411 147 274 2214
Fragments arranged by size:
2214 1421 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1775 1805 1775
Size: 30 5636
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 952 970 1118 1765 1777 2494 952
Size: 18 148 647 12 717 4124
Fragments arranged by size:
4124 717 647 148 18 12
BseYI C'CCAG_C
Cuts at: 3091 3849 4514 4922 3091
Size: 758 665 408 3835
Fragments arranged by size:
3835 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1970 1970
Size: 5666
BsiEI CG_ry'CG
Cuts at: 104 3208 3254 4484 104
Size: 3104 46 1230 1286
Fragments arranged by size:
3104 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2300 2680 3458 3648 4508 2300
Size: 380 778 190 860 3458
Fragments arranged by size:
3458 860 778 380 190
Bsp1286I G_dGCh'C
Cuts at: 2300 2680 3291 3384 3458 3648 3710 4508 2300
Size: 380 611 93 74 190 62 798 3458
Fragments arranged by size:
3458 798 611 380 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1434 1434
Size: 5666
BspHI T'CATG_A
Cuts at: 234 1653 2333 4098 234
Size: 1419 680 1765 1802
Fragments arranged by size:
1802 1765 1419 680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1857 2716 2930 3232 3613 1857
Size: 859 214 302 381 3910
Fragments arranged by size:
3910 859 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3958 4012 5496 5661 50
Size: 3908 54 1484 165 55
Fragments arranged by size:
3908 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1616 2405 3578 1616
Size: 789 1173 3704
Fragments arranged by size:
3704 1173 789
BsrFI r'CCGG_y
Cuts at: 3664 3845 3664
Size: 181 5485
BsrGI T'GTAC_A
Cuts at: 2402 2402
Size: 5666
BssSI C'ACGA_G
Cuts at: 1130 3937 4645 1130
Size: 2807 708 2151
Fragments arranged by size:
2807 2151 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1863 3043 1863
Size: 1180 4486
BstBI TT'CG_AA
Cuts at: 4027 4027
Size: 5666
BstYI r'GATC_y
Cuts at: 1 1503 2436 2691 3158 3181 3516 3762 4068
Size: 1502 933 255 467 23 335 246 306
Cuts at: 4068 4080 4166 5218 1
Size: 12 86 1052 449
Fragments arranged by size:
1502 1052 933 467 449 335 306 255
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1547 1547
Size: 5666
BtgI C'CryG_G
Cuts at: 263 263
Size: 5666
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3616 3797 3960 3616
Size: 181 163 5322
Fragments arranged by size:
5322 181 163
BtsI GCAGTG_nn'
Cuts at: 2909 2909
Size: 5666
ClaI AT'CG_AT
Cuts at: 422 422
Size: 5666
DraI TTT'AAA
Cuts at: 1224 1686 2665 1224
Size: 462 979 4225
Fragments arranged by size:
4225 979 462
DrdI GACnn_nn'nnGTC
Cuts at: 3372 4716 3372
Size: 1344 4322
EaeI y'GGCC_r
Cuts at: 3251 3425 3816 3843 5188 3251
Size: 174 391 27 1345 3729
Fragments arranged by size:
3729 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3251 3251
Size: 5666
EarI CTCTTCn'nnn_
Cuts at: 648 723 929 947 1289 1688 1742 1754 1949
Size: 75 206 18 342 399 54 12 195
Cuts at: 1949 2063 2075 3689 3899 648
Size: 114 12 1614 210 2415
Fragments arranged by size:
2415 1614 399 342 210 206 195 114
75 54 18 12 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3874 4616 4762 5476 3874
Size: 742 146 714 4064
Fragments arranged by size:
4064 742 714 146
EcoICRI GAG'CTC
Cuts at: 2678 2678
Size: 5666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 887 1067 1250 1355 1420 2087 2147 2174
Size: 43 180 183 105 65 667 60 27
Cuts at: 2174 3491 3923 4276 4938 844
Size: 1317 432 353 662 1572
Fragments arranged by size:
1572 1317 667 662 432 353 183 180
105 65 60 43 27
EcoO109I rG'GnC_Cy
Cuts at: 162 2805 162
Size: 2643 3023
EcoRI G'AATT_C
Cuts at: 2670 2670
Size: 5666
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2289 3685 2104
Size: 185 1396 4085
Fragments arranged by size:
4085 1396 185
FspI TGC'GCA
Cuts at: 3447 3447
Size: 5666
HaeII r_GCGC'y
Cuts at: 282 3100 3348 4578 5349 282
Size: 2818 248 1230 771 599
Fragments arranged by size:
2818 1230 771 599 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 853 885 1134 1166 1345 1368 1377 1400 1543
Size: 32 249 32 179 23 9 23 143
Cuts at: 1543 1575 2170 2202 3508 3538 3540 3570 853
Size: 32 595 32 1306 30 2 30 2949
Fragments arranged by size:
2949 1306 595 249 179 143 32 32
32 32 30 30 23 23 9 2
HincII GTy'rAC
Cuts at: 2379 2705 2379
Size: 326 5340
HpaI GTT'AAC
Cuts at: 2379 2379
Size: 5666
KasI G'GCGC_C
Cuts at: 278 3096 3344 278
Size: 2818 248 2600
Fragments arranged by size:
2818 2600 248
KpnI G_GTAC'C
Cuts at: 2686 2686
Size: 5666
MluI A'CGCG_T
Cuts at: 5099 5099
Size: 5666
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4425 4609 4425
Size: 184 5482
MscI TGG'CCA
Cuts at: 3427 3427
Size: 5666
MslI CAynn'nnrTG
Cuts at: 3655 3782 3655
Size: 127 5539
MspA1I CmG'CkG
Cuts at: 141 2777 3091 3451 4235 4480 141
Size: 2636 314 360 784 245 1327
Fragments arranged by size:
2636 1327 784 360 314 245
NaeI GCC'GGC
Cuts at: 3847 3847
Size: 5666
NarI GG'CG_CC
Cuts at: 279 3097 3345 279
Size: 2818 248 2600
Fragments arranged by size:
2818 2600 248
NgoMIV G'CCGG_C
Cuts at: 3845 3845
Size: 5666
NspI r_CATG'y
Cuts at: 1102 2719 3219 4822 1102
Size: 1617 500 1603 1946
Fragments arranged by size:
1946 1617 1603 500
PciI A'CATG_T
Cuts at: 4818 4818
Size: 5666
PmeI GTTT'AAAC
Cuts at: 2665 2665
Size: 5666
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 4112
Size: 32 5634
PpuMI rG'GwC_Cy
Cuts at: 162 162
Size: 5666
PstI C_TGCA'G
Cuts at: 2713 3398 2713
Size: 685 4981
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5666
PvuII CAG'CTG
Cuts at: 3091 3451 3091
Size: 360 5306
RsrII CG'GwC_CG
Cuts at: 3861 3861
Size: 5666
SacI G_AGCT'C
Cuts at: 2680 2680
Size: 5666
SalI G'TCGA_C
Cuts at: 2703 2703
Size: 5666
SapI GCTCTTCn'nnn_
Cuts at: 1289 1688 3689 3899 1289
Size: 399 2001 210 3056
Fragments arranged by size:
3056 2001 399 210
SbfI CC_TGCA'GG
Cuts at: 2713 2713
Size: 5666
ScaI AGT'ACT
Cuts at: 1405 5183 1405
Size: 3778 1888
SfcI C'TryA_G
Cuts at: 33 299 509 635 2709 3394 4362 4553 33
Size: 266 210 126 2074 685 968 191 1146
Fragments arranged by size:
2074 1146 968 685 266 210 191 126
SfoI GGC'GCC
Cuts at: 280 3098 3346 280
Size: 2818 248 2600
Fragments arranged by size:
2818 2600 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5666
SmaI CCC'GGG
Cuts at: 2688 2688
Size: 5666
SmlI C'TyrA_G
Cuts at: 2476 2821 3736 4450 4712 2476
Size: 345 915 714 262 3430
Fragments arranged by size:
3430 915 714 345 262
SpeI A'CTAG_T
Cuts at: 67 1039 67
Size: 972 4694
SphI G_CATG'C
Cuts at: 1102 2719 3219 1102
Size: 1617 500 3549
Fragments arranged by size:
3549 1617 500
SspI AAT'ATT
Cuts at: 1244 1594 1244
Size: 350 5316
StyI C'CwwG_G
Cuts at: 1999 2800 3154 1999
Size: 801 354 4511
Fragments arranged by size:
4511 801 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3249 3382 3841 4050 3249
Size: 133 459 209 4865
Fragments arranged by size:
4865 459 209 133
TatI w'GTAC_w
Cuts at: 1403 2402 5181 1403
Size: 999 2779 1888
Fragments arranged by size:
2779 1888 999
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5183 5215 5183
Size: 32 5634
Tth111I GACn'n_nGTC
Cuts at: 3463 3463
Size: 5666
XbaI T'CTAG_A
Cuts at: 1363 2697 1363
Size: 1334 4332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1352 3161 1352
Size: 1809 3857
XmaI C'CCGG_G
Cuts at: 2686 2686
Size: 5666
XmnI GAAnn'nnTTC
Cuts at: 2272 2272
Size: 5666
ZraI GAC'GTC
Cuts at: 1361 1361
Size: 5666
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI