Restriction map for
pF1KB7310
(Circular) (Six-base) MAPSORT of: pF1KB7310.seq Check: 2555 from: 1 to: 7937
pF1KB7310 7937 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2212 2485 2634 3501 3679 3870 2212
Size: 273 149 867 178 191 6279
Fragments arranged by size:
6279 867 273 191 178 149
AccI GT'mk_AC
Cuts at: 1211 3464 4975 1211
Size: 2253 1511 4173
Fragments arranged by size:
4173 2253 1511
Acc65I G'GTAC_C
Cuts at: 3160 4953 3160
Size: 1793 6144
AclI AA'CG_TT
Cuts at: 7777 7777
Size: 7937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 720 2599 3911 4141 5762 6194 6941 7209 720
Size: 1879 1312 230 1621 432 747 268 1448
Fragments arranged by size:
1879 1621 1448 1312 747 432 268 230
AfeI AGC'GCT
Cuts at: 4180 4180
Size: 7937
AflIII A'CryG_T
Cuts at: 5289 6393 7370 5289
Size: 1104 977 5856
Fragments arranged by size:
5856 1104 977
AgeI A'CCGG_T
Cuts at: 6325 6325
Size: 7937
AhdI GACnn_n'nnGTC
Cuts at: 2304 2304
Size: 7937
AleI CACnn'nnGTG
Cuts at: 2128 2155 3124 2128
Size: 27 969 6941
Fragments arranged by size:
6941 969 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2173 2564 2920 2990 2173
Size: 391 356 70 7120
Fragments arranged by size:
7120 391 356 70
AlwNI CAG_nnn'CTG
Cuts at: 1112 1233 1318 1998 2500 2644 2859 2931 2962
Size: 121 85 680 502 144 215 72 31
Cuts at: 2962 3273 3529 3729 3769 3954 4242 5242 6809
Size: 311 256 200 40 185 288 1000 1567
Cuts at: 6809 1112
Size: 2240
Fragments arranged by size:
2240 1567 1000 680 502 311 288 256
215 200 185 144 121 85 72 40
31
ApaI G_GGCC'C
Cuts at: 756 836 1658 1816 2085 2424 2463 3209 3248
Size: 80 822 158 269 339 39 746 39
Cuts at: 3248 3520 3892 4524 4777 756
Size: 272 372 632 253 3916
Fragments arranged by size:
3916 822 746 632 372 339 272 269
253 158 80 39 39
ApaLI G'TGCA_C
Cuts at: 6707 6707
Size: 7937
ApoI r'AATT_y
Cuts at: 3910 4941 7290 7546 3910
Size: 1031 2349 256 4301
Fragments arranged by size:
4301 2349 1031 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7937
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7937
AvaI C'yCGr_G
Cuts at: 1163 1370 2252 2409 2838 4256 4318 4957 1163
Size: 207 882 157 429 1418 62 639 4143
Fragments arranged by size:
4143 1418 882 639 429 207 157 62
BamHI G'GATC_C
Cuts at: 1662 1887 4962 1662
Size: 225 3075 4637
Fragments arranged by size:
4637 3075 225
BanI G'GyrC_C
Cuts at: 240 746 1330 1483 2224 2256 2373 2404 2548
Size: 506 584 153 741 32 117 31 144
Cuts at: 2548 2622 2974 3012 3039 3160 3691 4479 4732
Size: 74 352 38 27 121 531 788 253
Cuts at: 4732 4953 5367 5615 5650 240
Size: 221 414 248 35 2527
Fragments arranged by size:
2527 788 741 584 531 506 414 352
253 248 221 153 144 121 117 74
38 35 32 31 27
BanII G_rGCy'C
Cuts at: 424 521 756 836 1052 1084 1299 1649 1658
Size: 97 235 80 216 32 215 350 9
Cuts at: 1658 1768 1816 2085 2269 2424 2463 2488 2947
Size: 110 48 269 184 155 39 25 459
Cuts at: 2947 3055 3209 3217 3248 3520 3892 4273 4379
Size: 108 154 8 31 272 372 381 106
Cuts at: 4379 4524 4777 4881 4951 5981 424
Size: 145 253 104 70 1030 2380
Fragments arranged by size:
2380 1030 459 381 372 350 272 269
253 235 216 215 184 155 154 145
110 108 106 104 97 80 70 48
39 32 31 25 9 8
BbeI G_GCGC'C
Cuts at: 2377 5371 5619 2377
Size: 2994 248 4695
Fragments arranged by size:
4695 2994 248
BbsI GAAGACnn'nnnn_
Cuts at: 4132 4359 4132
Size: 227 7710
BbvCI CC'TCA_GC
Cuts at: 1734 2198 3420 3866 4700 1734
Size: 464 1222 446 834 4971
Fragments arranged by size:
4971 1222 834 464 446
BciVI GTATCCnnnnn_n'
Cuts at: 5835 6596 7937 5835
Size: 761 1341 5835
Fragments arranged by size:
5835 1341 761
BclI T'GATC_A
Cuts at: 5457 5457
Size: 7937
BglI GCCn_nnn'nGGC
Cuts at: 1199 1339 1653 2080 2458 3610 4588 1199
Size: 140 314 427 378 1152 978 4548
Fragments arranged by size:
4548 1152 978 427 378 314 140
BglII A'GATC_T
Cuts at: 5452 7489 5452
Size: 2037 5900
BlpI GC'TnA_GC
Cuts at: 1152 2847 5049 1152
Size: 1695 2202 4040
Fragments arranged by size:
4040 2202 1695
Bme1580I G_kGCm'C
Cuts at: 636 756 836 983 1021 1658 1816 2051 2085
Size: 120 80 147 38 637 158 235 34
Cuts at: 2085 2162 2245 2362 2407 2424 2463 2553 2716
Size: 77 83 117 45 17 39 90 163
Cuts at: 2716 2979 3146 3209 3248 3335 3520 3892 4524
Size: 263 167 63 39 87 185 372 632
Cuts at: 4524 4777 5562 5655 6711 636
Size: 253 785 93 1056 1862
Fragments arranged by size:
1862 1056 785 637 632 372 263 253
235 185 167 163 158 147 120 117
93 90 87 83 80 77 63 45
39 39 38 34 17
BmgBI CAC'GTC
Cuts at: 4452 4452
Size: 7937
BmrI ACTGGGnnnn_n'
Cuts at: 295 615 1281 1355 1771 2255 2802 3329 3736
Size: 320 666 74 416 484 547 527 407
Cuts at: 3736 5149 5230 5565 7709 295
Size: 1413 81 335 2144 523
Fragments arranged by size:
2144 1413 666 547 527 523 484 416
407 335 320 81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 516 6304 516
Size: 5788 2149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 1283 1954 3751 3976 3986 4344 4715 422
Size: 861 671 1797 225 10 358 371 3644
Fragments arranged by size:
3644 1797 861 671 371 358 225 10
Bpu10I CC'TnA_GC
Cuts at: 389 1078 1643 1734 1767 2198 2487 3049 3420
Size: 689 565 91 33 431 289 562 371
Cuts at: 3420 3866 4700 389
Size: 446 834 3626
Fragments arranged by size:
3626 834 689 565 562 446 431 371
289 91 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 3796 5113 5992 6484 6782 1486
Size: 2310 1317 879 492 298 2641
Fragments arranged by size:
2641 2310 1317 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1905 2255 3811 3889 7641 1905
Size: 350 1556 78 3752 2201
Fragments arranged by size:
3752 2201 1556 350 78
BsaAI yAC'GTr
Cuts at: 5290 5290
Size: 7937
BsaBI GATnn'nnATC
Cuts at: 6 5451 7488 6
Size: 5445 2037 455
Fragments arranged by size:
5445 2037 455
BsaHI Gr'CG_yC
Cuts at: 2203 2374 5368 5616 6318 7814 2203
Size: 171 2994 248 702 1496 2326
Fragments arranged by size:
2994 2326 1496 702 248 171
BsaWI w'CCGG_w
Cuts at: 788 5126 5647 6325 6599 6746 7157 788
Size: 4338 521 678 274 147 411 1568
Fragments arranged by size:
4338 1568 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1080 1110 2030 2060 3362 3392 3810 3840 4453
Size: 30 920 30 1302 30 418 30 613
Cuts at: 4453 4483 1080
Size: 30 4534
Fragments arranged by size:
4534 1302 920 613 418 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 505 508 1435 1456 1698 1722 1991 2035 3096
Size: 3 927 21 242 24 269 44 1061
Cuts at: 3096 3408 3883 3971 4225 4479 4697 505
Size: 312 475 88 254 254 218 3745
Fragments arranged by size:
3745 1061 927 475 312 269 254 254
242 218 88 44 24 21 3
BseYI C'CCAG_C
Cuts at: 244 281 1840 1969 2085 2274 2355 2500 2563
Size: 37 1559 129 116 189 81 145 63
Cuts at: 2563 2606 2631 2665 2852 2978 2986 3028 3054
Size: 43 25 34 187 126 8 42 26
Cuts at: 3054 3240 3247 3341 3434 3543 3565 3614 3622
Size: 186 7 94 93 109 22 49 8
Cuts at: 3622 3916 3985 3996 4759 4792 4868 4883 5362
Size: 294 69 11 763 33 76 15 479
Cuts at: 5362 6120 6697 7193 244
Size: 758 577 496 988
Fragments arranged by size:
1559 988 763 758 577 496 479 294
189 187 186 145 129 126 116 109
94 93 81 76 69 63 49 43
42 37 34 33 26 25 22 15
11 8 8 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 2660 2732 2845 2986 3148 3286 3380 3392
Size: 2374 72 113 141 162 138 94 12
Cuts at: 3392 3698 286
Size: 306 4525
Fragments arranged by size:
4525 2374 306 162 141 138 113 94
72 12
BsiEI CG_ry'CG
Cuts at: 104 5479 5525 6733 104
Size: 5375 46 1208 1308
Fragments arranged by size:
5375 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1052 1084 1299 3025 3217 4951 5729 5919 6711
Size: 32 215 1726 192 1734 778 190 792
Cuts at: 6711 1052
Size: 2278
Fragments arranged by size:
2278 1734 1726 792 778 215 192 190
32
BsiWI C'GTAC_G
Cuts at: 3200 3200
Size: 7937
BsmI GAATG_Cn'
Cuts at: 726 3665 726
Size: 2939 4998
BsmBI CGTCTCn'nnnn_
Cuts at: 982 1006 2210 982
Size: 24 1204 6709
Fragments arranged by size:
6709 1204 24
Bsp1286I G_dGCh'C
Cuts at: 424 521 636 756 836 983 1021 1052 1084
Size: 97 115 120 80 147 38 31 32
Cuts at: 1084 1299 1649 1658 1768 1816 2051 2085 2162
Size: 215 350 9 110 48 235 34 77
Cuts at: 2162 2245 2269 2362 2407 2424 2463 2488 2553
Size: 83 24 93 45 17 39 25 65
Cuts at: 2553 2716 2947 2979 3025 3055 3146 3209 3217
Size: 163 231 32 46 30 91 63 8
Cuts at: 3217 3248 3335 3520 3892 4273 4379 4524 4777
Size: 31 87 185 372 381 106 145 253
Cuts at: 4777 4881 4951 5562 5655 5729 5919 5981 6711
Size: 104 70 611 93 74 190 62 730
Cuts at: 6711 424
Size: 1650
Fragments arranged by size:
1650 730 611 381 372 350 253 235
231 215 190 185 163 147 145 120
115 110 106 104 97 93 93 91
87 83 80 77 74 70 65 63
62 48 46 45 39 38 34 32
32 31 31 30 25 24 17 9
8
BspEI T'CCGG_A
Cuts at: 7157 7157
Size: 7937
BspHI T'CATG_A
Cuts at: 351 728 7113 351
Size: 377 6385 1175
Fragments arranged by size:
6385 1175 377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 297 336 2212 2485 2634 3501 3568 3679 3870
Size: 39 1876 273 149 867 67 111 191
Cuts at: 3870 4987 5201 5503 5884 297
Size: 1117 214 302 381 2350
Fragments arranged by size:
2350 1876 1117 867 381 302 273 214
191 149 111 67 39
BsrBI CCG'CTC
Cuts at: 50 393 999 1515 3873 6229 6283 7767 7932
Size: 343 606 516 2358 2356 54 1484 165
Cuts at: 7932 50
Size: 55
Fragments arranged by size:
2358 2356 1484 606 516 343 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1463 2447 5849 1463
Size: 984 3402 3551
Fragments arranged by size:
3551 3402 984
BsrFI r'CCGG_y
Cuts at: 2920 5935 6116 6325 2920
Size: 3015 181 209 4532
Fragments arranged by size:
4532 3015 209 181
BssSI C'ACGA_G
Cuts at: 957 3210 6208 6566 957
Size: 2253 2998 358 2328
Fragments arranged by size:
2998 2328 2253 358
BstAPI GCAn_nnn'nTGC
Cuts at: 346 3150 3273 5314 346
Size: 2804 123 2041 2969
Fragments arranged by size:
2969 2804 2041 123
BstBI TT'CG_AA
Cuts at: 4172 6298 4172
Size: 2126 5811
BstEII G'GTnAC_C
Cuts at: 1518 2121 1518
Size: 603 7334
BstXI CCAn_nnnn'nTGG
Cuts at: 963 1792 3036 3821 4305 963
Size: 829 1244 785 484 4595
Fragments arranged by size:
4595 1244 829 785 484
BstYI r'GATC_y
Cuts at: 1 584 803 1662 1887 4637 4962 5429 5452
Size: 583 219 859 225 2750 325 467 23
Cuts at: 5452 5787 6033 7045 7131 7143 7489 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2750 1012 859 583 467 449 346 335
325 246 225 219 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 4208 4208
Size: 7937
BtgI C'CryG_G
Cuts at: 156 485 2117 3671 3830 3899 4298 4548 4679
Size: 329 1632 1554 159 69 399 250 131
Cuts at: 4679 156
Size: 3414
Fragments arranged by size:
3414 1632 1554 399 329 250 159 131
69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1941 2148 3642 5887 6068 6231 1941
Size: 207 1494 2245 181 163 3647
Fragments arranged by size:
3647 2245 1494 207 181 163
BtsI GCAGTG_nn'
Cuts at: 1019 1620 2477 2744 2929 3295 3533 3850 4044
Size: 601 857 267 185 366 238 317 194
Cuts at: 4044 4828 5180 1019
Size: 784 352 3776
Fragments arranged by size:
3776 857 784 601 366 352 317 267
238 194 185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 428 463 886 921 1476 1511 428
Size: 35 423 35 555 35 6854
Fragments arranged by size:
6854 555 423 35 35 35
DraI TTT'AAA
Cuts at: 4936 4936
Size: 7937
DraIII CAC_nnn'GTG
Cuts at: 303 367 2340 303
Size: 64 1973 5900
Fragments arranged by size:
5900 1973 64
DrdI GACnn_nn'nnGTC
Cuts at: 3937 4150 5643 6501 3937
Size: 213 1493 858 5373
Fragments arranged by size:
5373 1493 858 213
EaeI y'GGCC_r
Cuts at: 358 1340 1394 2190 2885 2922 3473 3902 4244
Size: 982 54 796 695 37 551 429 342
Cuts at: 4244 5522 5696 6087 6114 7459 358
Size: 1278 174 391 27 1345 836
Fragments arranged by size:
1345 1278 982 836 796 695 551 429
391 342 174 54 37 27
EagI C'GGCC_G
Cuts at: 5522 5522
Size: 7937
EarI CTCTTCn'nnn_
Cuts at: 4169 4279 5960 6170 4169
Size: 110 1681 210 5936
Fragments arranged by size:
5936 1681 210 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 464 1217 1686 2043 2051 2778 6145 6455 6601
Size: 753 469 357 8 727 3367 310 146
Cuts at: 6601 7747 464
Size: 1146 654
Fragments arranged by size:
3367 1146 753 727 654 469 357 310
146 8
EcoICRI GAG'CTC
Cuts at: 1050 1082 1297 3215 4949 1050
Size: 32 215 1918 1734 4038
Fragments arranged by size:
4038 1918 1734 215 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 720 1283 1954 2599 3751 3911 3976 3986
Size: 298 563 671 645 1152 160 65 10
Cuts at: 3986 4141 4344 4715 5762 6194 6941 7209 422
Size: 155 203 371 1047 432 747 268 1150
Fragments arranged by size:
1152 1150 1047 747 671 645 563 432
371 298 268 203 160 155 65 10
EcoNI CCTnn'n_nnAGG
Cuts at: 640 2557 640
Size: 1917 6020
EcoO109I rG'GnC_Cy
Cuts at: 125 427 753 832 1144 1303 1554 1654 1655
Size: 302 326 79 312 159 251 100 1
Cuts at: 1655 1813 1945 2081 2082 2420 2421 2459 2460
Size: 158 132 136 1 338 1 38 1
Cuts at: 2460 2829 2871 3093 3244 3373 3447 3516 3633
Size: 369 42 222 151 129 74 69 117
Cuts at: 3633 3677 3888 3889 3951 4152 4323 4521 4773
Size: 44 211 1 62 201 171 198 252
Cuts at: 4773 4774 4852 4906 5076 125
Size: 1 78 54 170 2986
Fragments arranged by size:
2986 369 338 326 312 302 252 251
222 211 201 198 171 170 159 158
151 136 132 129 117 100 79 78
74 69 62 54 44 42 38 1
1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4941 4941
Size: 7937
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4146 5956 4146
Size: 1810 6127
FseI GG_CCGG'CC
Cuts at: 2924 2924
Size: 7937
FspI TGC'GCA
Cuts at: 5718 5718
Size: 7937
HaeII r_GCGC'y
Cuts at: 1225 2377 3572 3884 4182 4518 5371 5619 6641
Size: 1152 1195 312 298 336 853 248 1022
Cuts at: 6641 7620 1225
Size: 979 1542
Fragments arranged by size:
1542 1195 1152 1022 979 853 336 312
298 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1039 1071 1970 2002 2290 2322 3362 3394 3810
Size: 32 899 32 288 32 1040 32 416
Cuts at: 3810 3842 4315 4347 4771 4803 5779 5809 5811
Size: 32 473 32 424 32 976 30 2
Cuts at: 5811 5841 1039
Size: 30 3135
Fragments arranged by size:
3135 1040 976 899 473 424 416 288
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4976 4976
Size: 7937
KasI G'GCGC_C
Cuts at: 2373 5367 5615 2373
Size: 2994 248 4695
Fragments arranged by size:
4695 2994 248
KpnI G_GTAC'C
Cuts at: 3164 4957 3164
Size: 1793 6144
MluI A'CGCG_T
Cuts at: 7370 7370
Size: 7937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 335 595 989 1561 6608 6792 335
Size: 260 394 572 5047 184 1480
Fragments arranged by size:
5047 1480 572 394 260 184
MscI TGG'CCA
Cuts at: 3475 3904 4246 5698 3475
Size: 429 342 1452 5714
Fragments arranged by size:
5714 1452 429 342
MslI CAynn'nnrTG
Cuts at: 172 554 2128 2155 2902 3124 3670 5926 6053
Size: 382 1574 27 747 222 546 2256 127
Cuts at: 6053 172
Size: 2056
Fragments arranged by size:
2256 2056 1574 747 546 382 222 127
27
MspA1I CmG'CkG
Cuts at: 343 1181 1359 1810 1973 2274 2659 2669 2856
Size: 838 178 451 163 301 385 10 187
Cuts at: 2856 3003 3032 3136 3251 3345 3618 4006 4364
Size: 147 29 104 115 94 273 388 358
Cuts at: 4364 4738 4918 5048 5362 5722 6735 6980 343
Size: 374 180 130 314 360 1013 245 1300
Fragments arranged by size:
1300 1013 838 451 388 385 374 360
358 314 301 273 245 187 180 178
163 147 130 115 104 94 29 10
NaeI GCC'GGC
Cuts at: 2922 6118 2922
Size: 3196 4741
NarI GG'CG_CC
Cuts at: 2374 5368 5616 2374
Size: 2994 248 4695
Fragments arranged by size:
4695 2994 248
NcoI C'CATG_G
Cuts at: 2117 3899 4548 2117
Size: 1782 649 5506
Fragments arranged by size:
5506 1782 649
NgoMIV G'CCGG_C
Cuts at: 2920 6116 2920
Size: 3196 4741
NspI r_CATG'y
Cuts at: 171 413 1008 2901 4990 5490 6397 171
Size: 242 595 1893 2089 500 907 1711
Fragments arranged by size:
2089 1893 1711 907 595 500 242
PasI CC'CwG_GG
Cuts at: 828 1033 1651 1834 2454 2529 2553 2644 3156
Size: 205 618 183 620 75 24 91 512
Cuts at: 3156 3943 828
Size: 787 4822
Fragments arranged by size:
4822 787 620 618 512 205 183 91
75 24
PciI A'CATG_T
Cuts at: 6393 6393
Size: 7937
PflMI CCAn_nnn'nTGG
Cuts at: 303 367 861 1834 2644 303
Size: 64 494 973 810 5596
Fragments arranged by size:
5596 973 810 494 64
PfoI T'CCnGG_A
Cuts at: 1299 1299
Size: 7937
PmeI GTTT'AAAC
Cuts at: 4936 4936
Size: 7937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7076 7108 7076
Size: 32 7905
PpuMI rG'GwC_Cy
Cuts at: 427 1144 1303 1554 2871 3093 3373 3677 4152
Size: 717 159 251 1317 222 280 304 475
Cuts at: 4152 4323 4906 427
Size: 171 583 3458
Fragments arranged by size:
3458 1317 717 583 475 304 280 251
222 171 159
PspOMI G'GGCC_C
Cuts at: 752 832 1654 1812 2081 2420 2459 3205 3244
Size: 80 822 158 269 339 39 746 39
Cuts at: 3244 3516 3888 4520 4773 752
Size: 272 372 632 253 3916
Fragments arranged by size:
3916 822 746 632 372 339 272 269
253 158 80 39 39
PstI C_TGCA'G
Cuts at: 1994 2777 3498 4238 4369 4631 4984 5669 1994
Size: 783 721 740 131 262 353 685 4262
Fragments arranged by size:
4262 783 740 721 685 353 262 131
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7937
PvuII CAG'CTG
Cuts at: 343 2856 3003 3345 4006 4364 4918 5362 5722
Size: 2513 147 342 661 358 554 444 360
Cuts at: 5722 343
Size: 2558
Fragments arranged by size:
2558 2513 661 554 444 360 358 342
147
RsrII CG'GwC_CG
Cuts at: 791 6132 791
Size: 5341 2596
SacI G_AGCT'C
Cuts at: 1052 1084 1299 3217 4951 1052
Size: 32 215 1918 1734 4038
Fragments arranged by size:
4038 1918 1734 215 32
SalI G'TCGA_C
Cuts at: 4974 4974
Size: 7937
SapI GCTCTTCn'nnn_
Cuts at: 4169 5960 6170 4169
Size: 1791 210 5936
Fragments arranged by size:
5936 1791 210
SbfI CC_TGCA'GG
Cuts at: 4984 4984
Size: 7937
ScaI AGT'ACT
Cuts at: 7454 7454
Size: 7937
SexAI A'CCwGG_T
Cuts at: 363 2544 363
Size: 2181 5756
SfcI C'TryA_G
Cuts at: 33 1225 1670 1990 2773 3218 3494 3591 4007
Size: 1192 445 320 783 445 276 97 416
Cuts at: 4007 4234 4365 4627 4980 5665 6658 6849 33
Size: 227 131 262 353 685 993 191 1121
Fragments arranged by size:
1192 1121 993 783 685 445 445 416
353 320 276 262 227 191 131 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 2080 3610 2080
Size: 1530 6407
SfoI GGC'GCC
Cuts at: 2375 5369 5617 2375
Size: 2994 248 4695
Fragments arranged by size:
4695 2994 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7937
SmaI CCC'GGG
Cuts at: 2411 2840 4258 4959 2411
Size: 429 1418 701 5389
Fragments arranged by size:
5389 1418 701 429
SmlI C'TyrA_G
Cuts at: 1501 3775 5092 6007 6499 6761 1501
Size: 2274 1317 915 492 262 2677
Fragments arranged by size:
2677 2274 1317 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7937
SphI G_CATG'C
Cuts at: 4990 5490 4990
Size: 500 7437
StuI AGG'CCT
Cuts at: 499 499
Size: 7937
StyI C'CwwG_G
Cuts at: 214 641 836 1091 1334 1862 2117 2651 3512
Size: 427 195 255 243 528 255 534 861
Cuts at: 3512 3608 3884 3899 4548 4616 5071 5425 214
Size: 96 276 15 649 68 455 354 2726
Fragments arranged by size:
2726 861 649 534 528 455 427 354
276 255 255 243 195 96 68 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 258 314 914 941 1537 1915 2266 2590 2694
Size: 56 600 27 596 378 351 324 104
Cuts at: 2694 2766 3042 3426 4806 5520 5653 6112 6321
Size: 72 276 384 1380 714 133 459 209
Cuts at: 6321 258
Size: 1874
Fragments arranged by size:
1874 1380 714 600 596 459 384 378
351 324 276 209 133 104 72 56
27
TatI w'GTAC_w
Cuts at: 2585 7452 2585
Size: 4867 3070
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 363 395 1080 1112 1586 1618 2030 2062 7454
Size: 32 685 32 474 32 412 32 5392
Cuts at: 7454 7486 363
Size: 32 814
Fragments arranged by size:
5392 814 685 474 412 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 621 5734 621
Size: 5113 2824
XbaI T'CTAG_A
Cuts at: 4968 4968
Size: 7937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 300 908 2235 2461 2860 3669 5432 300
Size: 608 1327 226 399 809 1763 2805
Fragments arranged by size:
2805 1763 1327 809 608 399 226
XmaI C'CCGG_G
Cuts at: 2409 2838 4256 4957 2409
Size: 429 1418 701 5389
Fragments arranged by size:
5389 1418 701 429
XmnI GAAnn'nnTTC
Cuts at: 724 724
Size: 7937
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BcgI BfrBI
BmtI BsrGI BssHII BstZ17I ClaI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI