Restriction map for
pF1KB7316
(Circular) (Six-base) MAPSORT of: pF1KB7316.seq Check: 2400 from: 1 to: 6404
pF1KB7316 6404 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 3442 3442
Size: 6404
Acc65I G'GTAC_C
Cuts at: 3420 3420
Size: 6404
AclI AA'CG_TT
Cuts at: 500 6244 500
Size: 5744 660
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1283 2927 4229 4661 5408 5676 1283
Size: 1644 1302 432 747 268 2011
Fragments arranged by size:
2011 1644 1302 747 432 268
AflIII A'CryG_T
Cuts at: 3756 4860 5837 3756
Size: 1104 977 4323
Fragments arranged by size:
4323 1104 977
AgeI A'CCGG_T
Cuts at: 4792 4792
Size: 6404
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1623 1655 1623
Size: 32 6372
AlwNI CAG_nnn'CTG
Cuts at: 192 2647 2896 2929 3709 5276 192
Size: 2455 249 33 780 1567 1320
Fragments arranged by size:
2455 1567 1320 780 249 33
ApaI G_GGCC'C
Cuts at: 1198 2569 1198
Size: 1371 5033
ApaLI G'TGCA_C
Cuts at: 5174 5174
Size: 6404
ApoI r'AATT_y
Cuts at: 180 404 444 478 534 1572 1758 1938 1982
Size: 224 40 34 56 1038 186 180 44
Cuts at: 1982 2292 2601 3219 3408 5757 6013 180
Size: 310 309 618 189 2349 256 571
Fragments arranged by size:
2349 1038 618 571 310 309 256 224
189 186 180 56 44 40 34
AseI AT'TA_AT
Cuts at: 20 1439 1493 1790 2614 3134 3296 20
Size: 1419 54 297 824 520 162 3128
Fragments arranged by size:
3128 1419 824 520 297 162 54
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6404
AvaI C'yCGr_G
Cuts at: 3424 3424
Size: 6404
BamHI G'GATC_C
Cuts at: 3429 3429
Size: 6404
BanI G'GyrC_C
Cuts at: 3420 3834 4082 4117 3420
Size: 414 248 35 5707
Fragments arranged by size:
5707 414 248 35
BanII G_rGCy'C
Cuts at: 255 659 826 1198 1951 2569 3418 4448 255
Size: 404 167 372 753 618 849 1030 2211
Fragments arranged by size:
2211 1030 849 753 618 404 372 167
BbeI G_GCGC'C
Cuts at: 3838 4086 3838
Size: 248 6156
BbsI GAAGACnn'nnnn_
Cuts at: 2348 2494 3338 2348
Size: 146 844 5414
Fragments arranged by size:
5414 844 146
BbvCI CC'TCA_GC
Cuts at: 1877 1877
Size: 6404
BciVI GTATCCnnnnn_n'
Cuts at: 839 2301 2683 4302 5063 6404 839
Size: 1462 382 1619 761 1341 839
Fragments arranged by size:
1619 1462 1341 839 761 382
BclI T'GATC_A
Cuts at: 3924 3924
Size: 6404
BglII A'GATC_T
Cuts at: 527 3919 5956 527
Size: 3392 2037 975
Fragments arranged by size:
3392 2037 975
BlpI GC'TnA_GC
Cuts at: 2897 3516 2897
Size: 619 5785
Bme1580I G_kGCm'C
Cuts at: 1198 2569 4029 4122 5178 1198
Size: 1371 1460 93 1056 2424
Fragments arranged by size:
2424 1460 1371 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2629 2884 3616 3697 4032 6176 2629
Size: 255 732 81 335 2144 2857
Fragments arranged by size:
2857 2144 732 335 255 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3288 4771 3288
Size: 1483 4921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 735 840 2148 3026 3215 506
Size: 229 105 1308 878 189 3695
Fragments arranged by size:
3695 1308 878 229 189 105
Bpu10I CC'TnA_GC
Cuts at: 1877 1877
Size: 6404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 860 3041 3580 4459 4951 5249 860
Size: 2181 539 879 492 298 2015
Fragments arranged by size:
2181 2015 879 539 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 805 6108 805
Size: 5303 1101
BsaAI yAC'GTr
Cuts at: 123 640 1201 3757 123
Size: 517 561 2556 2770
Fragments arranged by size:
2770 2556 561 517
BsaBI GATnn'nnATC
Cuts at: 6 1365 3918 5955 6
Size: 1359 2553 2037 455
Fragments arranged by size:
2553 2037 1359 455
BsaHI Gr'CG_yC
Cuts at: 3835 4083 4785 6281 3835
Size: 248 702 1496 3958
Fragments arranged by size:
3958 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3593 4114 4792 5066 5213 5624 3593
Size: 521 678 274 147 411 4373
Fragments arranged by size:
4373 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 245 1103 2029 3368 245
Size: 858 926 1339 3281
Fragments arranged by size:
3281 1339 926 858
BseYI C'CCAG_C
Cuts at: 1353 2988 3829 4587 5164 5660 1353
Size: 1635 841 758 577 496 2097
Fragments arranged by size:
2097 1635 841 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2999 2999
Size: 6404
BsiEI CG_ry'CG
Cuts at: 104 2107 3946 3992 5200 104
Size: 2003 1839 46 1208 1308
Fragments arranged by size:
2003 1839 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 659 2790 3418 4196 4386 5178 659
Size: 2131 628 778 190 792 1885
Fragments arranged by size:
2131 1885 792 778 628 190
BsmI GAATG_Cn'
Cuts at: 1344 1344
Size: 6404
BsmBI CGTCTCn'nnnn_
Cuts at: 2105 2105
Size: 6404
Bsp1286I G_dGCh'C
Cuts at: 255 659 826 1198 1951 2569 2790 3418 4029
Size: 404 167 372 753 618 221 628 611
Cuts at: 4029 4122 4196 4386 4448 5178 255
Size: 93 74 190 62 730 1481
Fragments arranged by size:
1481 753 730 628 618 611 404 372
221 190 167 93 74 62
BspEI T'CCGG_A
Cuts at: 5624 5624
Size: 6404
BspHI T'CATG_A
Cuts at: 258 5580 258
Size: 5322 1082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3454 3668 3970 4351 3454
Size: 214 302 381 5507
Fragments arranged by size:
5507 381 302 214
BsrBI CCG'CTC
Cuts at: 50 608 4696 4750 6234 6399 50
Size: 558 4088 54 1484 165 55
Fragments arranged by size:
4088 1484 558 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4316 4316
Size: 6404
BsrFI r'CCGG_y
Cuts at: 4402 4583 4792 4402
Size: 181 209 6014
Fragments arranged by size:
6014 209 181
BssSI C'ACGA_G
Cuts at: 4675 5033 4675
Size: 358 6046
BstAPI GCAn_nnn'nTGC
Cuts at: 3781 3781
Size: 6404
BstBI TT'CG_AA
Cuts at: 4765 4765
Size: 6404
BstXI CCAn_nnnn'nTGG
Cuts at: 985 985
Size: 6404
BstYI r'GATC_y
Cuts at: 1 527 3244 3429 3896 3919 4254 4500 5512
Size: 526 2717 185 467 23 335 246 1012
Cuts at: 5512 5598 5610 5956 1
Size: 86 12 346 449
Fragments arranged by size:
2717 1012 526 467 449 346 335 246
185 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3197 3197
Size: 6404
BtgI C'CryG_G
Cuts at: 330 330
Size: 6404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4354 4535 4698 4354
Size: 181 163 6060
Fragments arranged by size:
6060 181 163
BtsI GCAGTG_nn'
Cuts at: 2648 2790 3647 2648
Size: 142 857 5405
Fragments arranged by size:
5405 857 142
DraI TTT'AAA
Cuts at: 1145 3403 1145
Size: 2258 4146
DraIII CAC_nnn'GTG
Cuts at: 424 424
Size: 6404
DrdI GACnn_nn'nnGTC
Cuts at: 4110 4968 4110
Size: 858 5546
EaeI y'GGCC_r
Cuts at: 954 3989 4163 4554 4581 5926 954
Size: 3035 174 391 27 1345 1432
Fragments arranged by size:
3035 1432 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3989 3989
Size: 6404
EarI CTCTTCn'nnn_
Cuts at: 800 2569 3158 4427 4637 800
Size: 1769 589 1269 210 2567
Fragments arranged by size:
2567 1769 1269 589 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4612 4922 5068 6214 4612
Size: 310 146 1146 4802
Fragments arranged by size:
4802 1146 310 146
EcoICRI GAG'CTC
Cuts at: 657 3416 657
Size: 2759 3645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 735 840 1283 2148 2927 3026 3215 4229
Size: 229 105 443 865 779 99 189 1014
Cuts at: 4229 4661 5408 5676 506
Size: 432 747 268 1234
Fragments arranged by size:
1234 1014 865 779 747 443 432 268
229 189 105 99
EcoNI CCTnn'n_nnAGG
Cuts at: 3095 3095
Size: 6404
EcoO109I rG'GnC_Cy
Cuts at: 365 1720 3543 365
Size: 1355 1823 3226
Fragments arranged by size:
3226 1823 1355
EcoRI G'AATT_C
Cuts at: 3408 3408
Size: 6404
EcoRV GAT'ATC
Cuts at: 1099 2233 1099
Size: 1134 5270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 721 907 1232 4423 721
Size: 186 325 3191 2702
Fragments arranged by size:
3191 2702 325 186
FspI TGC'GCA
Cuts at: 4185 4185
Size: 6404
HaeII r_GCGC'y
Cuts at: 3838 4086 5108 6087 3838
Size: 248 1022 979 4155
Fragments arranged by size:
4155 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3025 3057 3319 3351 4246 4276 4278 4308 3025
Size: 32 262 32 895 30 2 30 5121
Fragments arranged by size:
5121 895 262 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3443 3443
Size: 6404
KasI G'GCGC_C
Cuts at: 3834 4082 3834
Size: 248 6156
KpnI G_GTAC'C
Cuts at: 3424 3424
Size: 6404
MluI A'CGCG_T
Cuts at: 5837 5837
Size: 6404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 365 1144 2087 2637 5075 5259 365
Size: 779 943 550 2438 184 1510
Fragments arranged by size:
2438 1510 943 779 550 184
MscI TGG'CCA
Cuts at: 956 4165 956
Size: 3209 3195
MslI CAynn'nnrTG
Cuts at: 983 2350 2635 4393 4520 983
Size: 1367 285 1758 127 2867
Fragments arranged by size:
2867 1758 1367 285 127
MspA1I CmG'CkG
Cuts at: 1353 1450 1545 3515 3829 4189 5202 5447 1353
Size: 97 95 1970 314 360 1013 245 2310
Fragments arranged by size:
2310 1970 1013 360 314 245 97 95
NaeI GCC'GGC
Cuts at: 4585 4585
Size: 6404
NarI GG'CG_CC
Cuts at: 3835 4083 3835
Size: 248 6156
NgoMIV G'CCGG_C
Cuts at: 4583 4583
Size: 6404
NspI r_CATG'y
Cuts at: 3457 3957 4864 3457
Size: 500 907 4997
Fragments arranged by size:
4997 907 500
PciI A'CATG_T
Cuts at: 4860 4860
Size: 6404
PfoI T'CCnGG_A
Cuts at: 142 1170 142
Size: 1028 5376
PmeI GTTT'AAAC
Cuts at: 3403 3403
Size: 6404
PmlI CAC'GTG
Cuts at: 1201 1201
Size: 6404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5543 5575 5543
Size: 32 6372
PpuMI rG'GwC_Cy
Cuts at: 365 365
Size: 6404
PspOMI G'GGCC_C
Cuts at: 1194 2565 1194
Size: 1371 5033
PstI C_TGCA'G
Cuts at: 2319 3162 3451 4136 2319
Size: 843 289 685 4587
Fragments arranged by size:
4587 843 685 289
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6404
PvuII CAG'CTG
Cuts at: 1450 3829 4189 1450
Size: 2379 360 3665
Fragments arranged by size:
3665 2379 360
RsrII CG'GwC_CG
Cuts at: 4599 4599
Size: 6404
SacI G_AGCT'C
Cuts at: 659 3418 659
Size: 2759 3645
SalI G'TCGA_C
Cuts at: 3441 3441
Size: 6404
SapI GCTCTTCn'nnn_
Cuts at: 4427 4637 4427
Size: 210 6194
SbfI CC_TGCA'GG
Cuts at: 3451 3451
Size: 6404
ScaI AGT'ACT
Cuts at: 5921 5921
Size: 6404
SexAI A'CCwGG_T
Cuts at: 2184 2531 2184
Size: 347 6057
SfcI C'TryA_G
Cuts at: 33 2315 3092 3158 3447 4132 5125 5316 33
Size: 2282 777 66 289 685 993 191 1121
Fragments arranged by size:
2282 1121 993 777 685 289 191 66
SfoI GGC'GCC
Cuts at: 3836 4084 3836
Size: 248 6156
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6404
SmaI CCC'GGG
Cuts at: 3426 3426
Size: 6404
SmlI C'TyrA_G
Cuts at: 875 3056 3559 4474 4966 5228 875
Size: 2181 503 915 492 262 2051
Fragments arranged by size:
2181 2051 915 503 492 262
SnaBI TAC'GTA
Cuts at: 123 640 123
Size: 517 5887
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6404
SphI G_CATG'C
Cuts at: 3457 3957 3457
Size: 500 5904
SspI AAT'ATT
Cuts at: 1017 2469 1017
Size: 1452 4952
StyI C'CwwG_G
Cuts at: 737 3538 3892 737
Size: 2801 354 3249
Fragments arranged by size:
3249 2801 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2093 2976 3987 4120 4579 4788 2093
Size: 883 1011 133 459 209 3709
Fragments arranged by size:
3709 1011 883 459 209 133
TatI w'GTAC_w
Cuts at: 1533 5919 1533
Size: 4386 2018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5921 5953 5921
Size: 32 6372
Tth111I GACn'n_nGTC
Cuts at: 2077 4201 2077
Size: 2124 4280
XbaI T'CTAG_A
Cuts at: 3435 3435
Size: 6404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 756 3899 756
Size: 3143 3261
XmaI C'CCGG_G
Cuts at: 3424 3424
Size: 6404
XmnI GAAnn'nnTTC
Cuts at: 2345 2345
Size: 6404
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SrfI StuI SwaI
XhoI ZraI