Restriction map for
pF1KB7319
(Circular) (Six-base) MAPSORT of: pF1KB7319.seq Check: 8793 from: 1 to: 5630
pF1KB7319 5630 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 261 1342 2002 261
Size: 1081 660 3889
Fragments arranged by size:
3889 1081 660
AatII G_ACGT'C
Cuts at: 859 859
Size: 5630
AccI GT'mk_AC
Cuts at: 1262 1352 2668 1262
Size: 90 1316 4224
Fragments arranged by size:
4224 1316 90
Acc65I G'GTAC_C
Cuts at: 2646 2646
Size: 5630
AclI AA'CG_TT
Cuts at: 5470 5470
Size: 5630
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1095 1552 1715 3455 3887 4634 4902 1095
Size: 457 163 1740 432 747 268 1823
Fragments arranged by size:
1823 1740 747 457 432 268 163
AflIII A'CryG_T
Cuts at: 2982 4086 5063 2982
Size: 1104 977 3549
Fragments arranged by size:
3549 1104 977
AgeI A'CCGG_T
Cuts at: 4018 4018
Size: 5630
AleI CACnn'nnGTG
Cuts at: 2476 2476
Size: 5630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 388 2305 2377 388
Size: 1917 72 3641
Fragments arranged by size:
3641 1917 72
AlwNI CAG_nnn'CTG
Cuts at: 876 2120 2935 4502 876
Size: 1244 815 1567 2004
Fragments arranged by size:
2004 1567 1244 815
ApaI G_GGCC'C
Cuts at: 116 1135 1912 2141 116
Size: 1019 777 229 3605
Fragments arranged by size:
3605 1019 777 229
ApaLI G'TGCA_C
Cuts at: 4400 4400
Size: 5630
ApoI r'AATT_y
Cuts at: 2634 4983 5239 2634
Size: 2349 256 3025
Fragments arranged by size:
3025 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5630
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
AvaI C'yCGr_G
Cuts at: 1104 2650 1104
Size: 1546 4084
BamHI G'GATC_C
Cuts at: 2655 2655
Size: 5630
BanI G'GyrC_C
Cuts at: 273 991 1066 1601 1675 1797 2646 3060 3308
Size: 718 75 535 74 122 849 414 248
Cuts at: 3308 3343 273
Size: 35 2560
Fragments arranged by size:
2560 849 718 535 414 248 122 75
74 35
BanII G_rGCy'C
Cuts at: 116 1135 1912 2141 2644 3674 116
Size: 1019 777 229 503 1030 2072
Fragments arranged by size:
2072 1030 1019 777 503 229
BbeI G_GCGC'C
Cuts at: 995 3064 3312 995
Size: 2069 248 3313
Fragments arranged by size:
3313 2069 248
BbsI GAAGACnn'nnnn_
Cuts at: 1770 1770
Size: 5630
BbvCI CC'TCA_GC
Cuts at: 1156 1840 2398 1156
Size: 684 558 4388
Fragments arranged by size:
4388 684 558
BciVI GTATCCnnnnn_n'
Cuts at: 3528 4289 5630 3528
Size: 761 1341 3528
Fragments arranged by size:
3528 1341 761
BclI T'GATC_A
Cuts at: 3150 3150
Size: 5630
BglI GCCn_nnn'nGGC
Cuts at: 1064 1316 1673 1822 1064
Size: 252 357 149 4872
Fragments arranged by size:
4872 357 252 149
BglII A'GATC_T
Cuts at: 1376 3145 5182 1376
Size: 1769 2037 1824
Fragments arranged by size:
2037 1824 1769
BlpI GC'TnA_GC
Cuts at: 164 2742 164
Size: 2578 3052
Bme1580I G_kGCm'C
Cuts at: 116 278 1069 1100 1135 1912 2141 3255 3348
Size: 162 791 31 35 777 229 1114 93
Cuts at: 3348 4404 116
Size: 1056 1342
Fragments arranged by size:
1342 1114 1056 791 777 229 162 93
35 31
BmgBI CAC'GTC
Cuts at: 1437 1641 1437
Size: 204 5426
BmrI ACTGGGnnnn_n'
Cuts at: 2130 2842 2923 3258 5402 2130
Size: 712 81 335 2144 2358
Fragments arranged by size:
2358 2144 712 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3997 3997
Size: 5630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1890 1890
Size: 5630
Bpu10I CC'TnA_GC
Cuts at: 1156 1840 2398 1156
Size: 684 558 4388
Fragments arranged by size:
4388 684 558
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 2806 3685 4177 4475 1390
Size: 1416 879 492 298 2545
Fragments arranged by size:
2545 1416 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1744 1992 5334 1744
Size: 248 3342 2040
Fragments arranged by size:
3342 2040 248
BsaAI yAC'GTr
Cuts at: 1971 2983 1971
Size: 1012 4618
BsaBI GATnn'nnATC
Cuts at: 6 3144 5181 6
Size: 3138 2037 455
Fragments arranged by size:
3138 2037 455
BsaHI Gr'CG_yC
Cuts at: 856 992 3061 3309 4011 5507 856
Size: 136 2069 248 702 1496 979
Fragments arranged by size:
2069 1496 979 702 248 136
BsaWI w'CCGG_w
Cuts at: 1475 2819 3340 4018 4292 4439 4850 1475
Size: 1344 521 678 274 147 411 2255
Fragments arranged by size:
2255 1344 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 553
Size: 30 5600
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 123 361 877 1303 1387 2631 123
Size: 238 516 426 84 1244 3122
Fragments arranged by size:
3122 1244 516 426 238 84
BseYI C'CCAG_C
Cuts at: 387 661 1141 1903 3055 3813 4390 4886 387
Size: 274 480 762 1152 758 577 496 1131
Fragments arranged by size:
1152 1131 762 758 577 496 480 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 712 1021 1288 1320 1429 2421 688
Size: 24 309 267 32 109 992 3897
Fragments arranged by size:
3897 992 309 267 109 32 24
BsiEI CG_ry'CG
Cuts at: 104 3172 3218 4426 104
Size: 3068 46 1208 1308
Fragments arranged by size:
3068 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 443 910 2501 2644 3422 3612 4404 443
Size: 467 1591 143 778 190 792 1669
Fragments arranged by size:
1669 1591 792 778 467 190 143
BsmI GAATG_Cn'
Cuts at: 1714 1714
Size: 5630
BsmBI CGTCTCn'nnnn_
Cuts at: 814 2151 2433 814
Size: 1337 282 4011
Fragments arranged by size:
4011 1337 282
Bsp1286I G_dGCh'C
Cuts at: 116 278 443 910 1069 1100 1135 1912 2141
Size: 162 165 467 159 31 35 777 229
Cuts at: 2141 2501 2644 3255 3348 3422 3612 3674 4404
Size: 360 143 611 93 74 190 62 730
Cuts at: 4404 116
Size: 1342
Fragments arranged by size:
1342 777 730 611 467 360 229 190
165 162 159 143 93 74 62 35
31
BspEI T'CCGG_A
Cuts at: 4850 4850
Size: 5630
BspHI T'CATG_A
Cuts at: 548 4806 548
Size: 4258 1372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 200 261 1189 1342 2002 2321 2680 2894 3196
Size: 61 928 153 660 319 359 214 302
Cuts at: 3196 3577 200
Size: 381 2253
Fragments arranged by size:
2253 928 660 381 359 319 302 214
153 61
BsrBI CCG'CTC
Cuts at: 50 3922 3976 5460 5625 50
Size: 3872 54 1484 165 55
Fragments arranged by size:
3872 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1474 3542 1474
Size: 2068 3562
BsrFI r'CCGG_y
Cuts at: 168 435 3628 3809 4018 168
Size: 267 3193 181 209 1780
Fragments arranged by size:
3193 1780 267 209 181
BssHII G'CGCG_C
Cuts at: 913 913
Size: 5630
BssSI C'ACGA_G
Cuts at: 457 3901 4259 457
Size: 3444 358 1828
Fragments arranged by size:
3444 1828 358
BstAPI GCAn_nnn'nTGC
Cuts at: 330 699 1664 3007 330
Size: 369 965 1343 2953
Fragments arranged by size:
2953 1343 965 369
BstBI TT'CG_AA
Cuts at: 3991 3991
Size: 5630
BstEII G'GTnAC_C
Cuts at: 879 2469 879
Size: 1590 4040
BstXI CCAn_nnnn'nTGG
Cuts at: 775 1347 1812 2520 775
Size: 572 465 708 3885
Fragments arranged by size:
3885 708 572 465
BstYI r'GATC_y
Cuts at: 1 1376 2655 3122 3145 3480 3726 4738 4824
Size: 1375 1279 467 23 335 246 1012 86
Cuts at: 4824 4836 5182 1
Size: 12 346 449
Fragments arranged by size:
1375 1279 1012 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 686 2204 107
Size: 579 1518 3533
Fragments arranged by size:
3533 1518 579
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 626 792 1791 3580 3761 3924 626
Size: 166 999 1789 181 163 2332
Fragments arranged by size:
2332 1789 999 181 166 163
BtsI GCAGTG_nn'
Cuts at: 832 1933 2873 832
Size: 1101 940 3589
Fragments arranged by size:
3589 1101 940
DraI TTT'AAA
Cuts at: 2629 2629
Size: 5630
DrdI GACnn_nn'nnGTC
Cuts at: 2488 3336 4194 2488
Size: 848 858 3924
Fragments arranged by size:
3924 858 848
EaeI y'GGCC_r
Cuts at: 557 753 765 1823 1850 2090 2201 2510 3215
Size: 196 12 1058 27 240 111 309 705
Cuts at: 3215 3389 3780 3807 5152 557
Size: 174 391 27 1345 1035
Fragments arranged by size:
1345 1058 1035 705 391 309 240 196
174 111 27 27 12
EagI C'GGCC_G
Cuts at: 3215 3215
Size: 5630
EarI CTCTTCn'nnn_
Cuts at: 226 2560 3653 3863 226
Size: 2334 1093 210 1993
Fragments arranged by size:
2334 1993 1093 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3838 4148 4294 5440 3838
Size: 310 146 1146 4028
Fragments arranged by size:
4028 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2642 2642
Size: 5630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1095 1552 1715 1890 3455 3887 4634 4902 1095
Size: 457 163 175 1565 432 747 268 1823
Fragments arranged by size:
1823 1565 747 457 432 268 175 163
EcoNI CCTnn'n_nnAGG
Cuts at: 322 322
Size: 5630
EcoO109I rG'GnC_Cy
Cuts at: 112 156 1131 1908 1909 2010 2137 2138 2769
Size: 44 975 777 1 101 127 1 631
Cuts at: 2769 112
Size: 2973
Fragments arranged by size:
2973 975 777 631 127 101 44 1
1
EcoRI G'AATT_C
Cuts at: 2634 2634
Size: 5630
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 651 1407 3649 651
Size: 756 2242 2632
Fragments arranged by size:
2632 2242 756
FspI TGC'GCA
Cuts at: 3411 3411
Size: 5630
HaeII r_GCGC'y
Cuts at: 605 668 870 876 995 3064 3312 4334 5313
Size: 63 202 6 119 2069 248 1022 979
Cuts at: 5313 605
Size: 922
Fragments arranged by size:
2069 1022 979 922 248 202 119 63
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1470 1502 3472 3502 3504 3534 1470
Size: 32 1970 30 2 30 3566
Fragments arranged by size:
3566 1970 32 30 30 2
HincII GTy'rAC
Cuts at: 1389 2669 1389
Size: 1280 4350
KasI G'GCGC_C
Cuts at: 991 3060 3308 991
Size: 2069 248 3313
Fragments arranged by size:
3313 2069 248
KpnI G_GTAC'C
Cuts at: 2650 2650
Size: 5630
MfeI C'AATT_G
Cuts at: 2255 2255
Size: 5630
MluI A'CGCG_T
Cuts at: 5063 5063
Size: 5630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 446 4301 4485 446
Size: 3855 184 1591
Fragments arranged by size:
3855 1591 184
MscI TGG'CCA
Cuts at: 559 767 1852 2092 2203 2512 3391 559
Size: 208 1085 240 111 309 879 2798
Fragments arranged by size:
2798 1085 879 309 240 208 111
MslI CAynn'nnrTG
Cuts at: 553 1810 1858 2443 2476 3619 3746 553
Size: 1257 48 585 33 1143 127 2437
Fragments arranged by size:
2437 1257 1143 585 127 48 33
MspA1I CmG'CkG
Cuts at: 696 713 771 1365 1530 1773 2459 2741 3055
Size: 17 58 594 165 243 686 282 314
Cuts at: 3055 3415 4428 4673 696
Size: 360 1013 245 1653
Fragments arranged by size:
1653 1013 686 594 360 314 282 245
243 165 58 17
NaeI GCC'GGC
Cuts at: 170 3811 170
Size: 3641 1989
NarI GG'CG_CC
Cuts at: 992 3061 3309 992
Size: 2069 248 3313
Fragments arranged by size:
3313 2069 248
NcoI C'CATG_G
Cuts at: 107 686 107
Size: 579 5051
NgoMIV G'CCGG_C
Cuts at: 168 3809 168
Size: 3641 1989
NspI r_CATG'y
Cuts at: 2199 2683 3183 4090 2199
Size: 484 500 907 3739
Fragments arranged by size:
3739 907 500 484
PasI CC'CwG_GG
Cuts at: 116 1830 116
Size: 1714 3916
PciI A'CATG_T
Cuts at: 4086 4086
Size: 5630
PflMI CCAn_nnn'nTGG
Cuts at: 876 1141 876
Size: 265 5365
PfoI T'CCnGG_A
Cuts at: 815 1400 1781 815
Size: 585 381 4664
Fragments arranged by size:
4664 585 381
PmeI GTTT'AAAC
Cuts at: 2629 2629
Size: 5630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2403 4769 4801 2371
Size: 32 2366 32 3200
Fragments arranged by size:
3200 2366 32 32
PpuMI rG'GwC_Cy
Cuts at: 156 156
Size: 5630
PspOMI G'GGCC_C
Cuts at: 112 1131 1908 2137 112
Size: 1019 777 229 3605
Fragments arranged by size:
3605 1019 777 229
PstI C_TGCA'G
Cuts at: 272 839 902 1523 2677 3362 272
Size: 567 63 621 1154 685 2540
Fragments arranged by size:
2540 1154 685 621 567 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5630
PvuII CAG'CTG
Cuts at: 696 771 1365 1530 1773 3055 3415 696
Size: 75 594 165 243 1282 360 2911
Fragments arranged by size:
2911 1282 594 360 243 165 75
RsrII CG'GwC_CG
Cuts at: 3825 3825
Size: 5630
SacI G_AGCT'C
Cuts at: 2644 2644
Size: 5630
SalI G'TCGA_C
Cuts at: 2667 2667
Size: 5630
SapI GCTCTTCn'nnn_
Cuts at: 3653 3863 3653
Size: 210 5420
SbfI CC_TGCA'GG
Cuts at: 272 2677 272
Size: 2405 3225
ScaI AGT'ACT
Cuts at: 5147 5147
Size: 5630
SfcI C'TryA_G
Cuts at: 33 268 489 597 835 898 1263 1519 2112
Size: 235 221 108 238 63 365 256 593
Cuts at: 2112 2454 2673 3358 4351 4542 33
Size: 342 219 685 993 191 1121
Fragments arranged by size:
1121 993 685 593 365 342 256 238
235 221 219 191 108 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 1822 1822
Size: 5630
SfoI GGC'GCC
Cuts at: 993 3062 3310 993
Size: 2069 248 3313
Fragments arranged by size:
3313 2069 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
SmaI CCC'GGG
Cuts at: 1106 2652 1106
Size: 1546 4084
SmlI C'TyrA_G
Cuts at: 1369 2785 3700 4192 4454 1369
Size: 1416 915 492 262 2545
Fragments arranged by size:
2545 1416 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5630
SphI G_CATG'C
Cuts at: 2199 2683 3183 2199
Size: 484 500 4646
Fragments arranged by size:
4646 500 484
StuI AGG'CCT
Cuts at: 1717 1717
Size: 5630
StyI C'CwwG_G
Cuts at: 107 686 1982 2764 3118 107
Size: 579 1296 782 354 2619
Fragments arranged by size:
2619 1296 782 579 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 202 675 1815 1902 3213 3346 3805 4014 202
Size: 473 1140 87 1311 133 459 209 1818
Fragments arranged by size:
1818 1311 1140 473 459 209 133 87
TatI w'GTAC_w
Cuts at: 1271 5145 1271
Size: 3874 1756
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5147 5179 5147
Size: 32 5598
Tth111I GACn'n_nGTC
Cuts at: 1030 3427 1030
Size: 2397 3233
XbaI T'CTAG_A
Cuts at: 2661 2661
Size: 5630
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 985 1809 3125 985
Size: 824 1316 3490
Fragments arranged by size:
3490 1316 824
XmaI C'CCGG_G
Cuts at: 1104 2650 1104
Size: 1546 4084
XmnI GAAnn'nnTTC
Cuts at: 1489 1489
Size: 5630
ZraI GAC'GTC
Cuts at: 857 857
Size: 5630
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsrGI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI