Restriction map for
pF1KB7322
(Circular) (Six-base) MAPSORT of: pF1KB7322.seq Check: 7635 from: 1 to: 10169
pF1KB7322 10169 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2409 3469 2409
Size: 1060 9109
AccI GT'mk_AC
Cuts at: 6706 6770 7207 6706
Size: 64 437 9668
Fragments arranged by size:
9668 437 64
Acc65I G'GTAC_C
Cuts at: 7185 7185
Size: 10169
AclI AA'CG_TT
Cuts at: 1874 4094 6645 10009 1874
Size: 2220 2551 3364 2034
Fragments arranged by size:
3364 2551 2220 2034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 722 1942 2009 2528 3170 3667 3725 3956
Size: 409 1220 67 519 642 497 58 231
Cuts at: 3956 5707 5918 7994 8426 9173 9441 313
Size: 1751 211 2076 432 747 268 1041
Fragments arranged by size:
2076 1751 1220 1041 747 642 519 497
432 409 268 231 211 67 58
AflII C'TTAA_G
Cuts at: 6024 6024
Size: 10169
AflIII A'CryG_T
Cuts at: 1044 1242 7521 8625 9602 1044
Size: 198 6279 1104 977 1611
Fragments arranged by size:
6279 1611 1104 977 198
AgeI A'CCGG_T
Cuts at: 8557 8557
Size: 10169
AleI CACnn'nnGTG
Cuts at: 5359 5359
Size: 10169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3065 3497 6926 3065
Size: 432 3429 6308
Fragments arranged by size:
6308 3429 432
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1859 1891 6630 6662 6663 6695 1859
Size: 32 4739 32 1 32 5333
Fragments arranged by size:
5333 4739 32 32 32 1
AlwNI CAG_nnn'CTG
Cuts at: 580 700 2974 3052 3468 3601 7474 9041 580
Size: 120 2274 78 416 133 3873 1567 1708
Fragments arranged by size:
3873 2274 1708 1567 416 133 120 78
ApaI G_GGCC'C
Cuts at: 193 6113 193
Size: 5920 4249
ApaLI G'TGCA_C
Cuts at: 4622 6548 8939 4622
Size: 1926 2391 5852
Fragments arranged by size:
5852 2391 1926
ApoI r'AATT_y
Cuts at: 1303 3355 4797 5232 5925 5943 6048 6231 7173
Size: 2052 1442 435 693 18 105 183 942
Cuts at: 7173 9522 9778 1303
Size: 2349 256 1694
Fragments arranged by size:
2349 2052 1694 1442 942 693 435 256
183 105 18
AseI AT'TA_AT
Cuts at: 20 1286 4721 20
Size: 1266 3435 5468
Fragments arranged by size:
5468 3435 1266
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10169
AvaI C'yCGr_G
Cuts at: 1631 2867 6537 7189 1631
Size: 1236 3670 652 4611
Fragments arranged by size:
4611 3670 1236 652
BamHI G'GATC_C
Cuts at: 4737 7194 4737
Size: 2457 7712
BanI G'GyrC_C
Cuts at: 436 1477 5190 5292 5799 6420 7125 7185 7599
Size: 1041 3713 102 507 621 705 60 414
Cuts at: 7599 7847 7882 436
Size: 248 35 2723
Fragments arranged by size:
3713 2723 1041 705 621 507 414 248
102 60 35
BanII G_rGCy'C
Cuts at: 166 193 243 2340 3079 3387 5155 6113 7183
Size: 27 50 2097 739 308 1768 958 1070
Cuts at: 7183 8213 166
Size: 1030 2122
Fragments arranged by size:
2122 2097 1768 1070 1030 958 739 308
50 27
BbeI G_GCGC'C
Cuts at: 7603 7851 7603
Size: 248 9921
BbsI GAAGACnn'nnnn_
Cuts at: 1091 2844 3154 5371 1091
Size: 1753 310 2217 5889
Fragments arranged by size:
5889 2217 1753 310
BbvCI CC'TCA_GC
Cuts at: 4791 4791
Size: 10169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 785 819 785
Size: 34 10135
BciVI GTATCCnnnnn_n'
Cuts at: 934 1893 6543 8067 8828 10169 934
Size: 959 4650 1524 761 1341 934
Fragments arranged by size:
4650 1524 1341 959 934 761
BclI T'GATC_A
Cuts at: 4730 7689 4730
Size: 2959 7210
BfrBI ATG'CAT
Cuts at: 975 975
Size: 10169
BglII A'GATC_T
Cuts at: 1971 7684 9721 1971
Size: 5713 2037 2419
Fragments arranged by size:
5713 2419 2037
BlpI GC'TnA_GC
Cuts at: 7281 7281
Size: 10169
Bme1580I G_kGCm'C
Cuts at: 193 1320 1482 4626 6096 6113 6423 6552 7128
Size: 1127 162 3144 1470 17 310 129 576
Cuts at: 7128 7794 7887 8943 193
Size: 666 93 1056 1419
Fragments arranged by size:
3144 1470 1419 1127 1056 666 576 310
162 129 93 17
BmrI ACTGGGnnnn_n'
Cuts at: 2781 4954 5809 7381 7462 7797 9941 2781
Size: 2173 855 1572 81 335 2144 3009
Fragments arranged by size:
3009 2173 2144 1572 855 335 81
BmtI G_CTAG'C
Cuts at: 1072 1583 3433 1072
Size: 511 1850 7808
Fragments arranged by size:
7808 1850 511
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3000 6317 8536 3000
Size: 3317 2219 4633
Fragments arranged by size:
4633 3317 2219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1157 3281 4070 4679 5774 6254 6965 7028 1157
Size: 2124 789 609 1095 480 711 63 4298
Fragments arranged by size:
4298 2124 1095 789 711 609 480 63
Bpu10I CC'TnA_GC
Cuts at: 1326 4484 4791 5177 1326
Size: 3158 307 386 6318
Fragments arranged by size:
6318 3158 386 307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1691 1808 3294 3884 7345 8224 8716 9014 1691
Size: 117 1486 590 3461 879 492 298 2846
Fragments arranged by size:
3461 2846 1486 879 590 492 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 3214 4655 6288 9873 3214
Size: 1441 1633 3585 3510
Fragments arranged by size:
3585 3510 1633 1441
BsaAI yAC'GTr
Cuts at: 3400 6655 7522 3400
Size: 3255 867 6047
Fragments arranged by size:
6047 3255 867
BsaBI GATnn'nnATC
Cuts at: 6 5550 7683 9720 6
Size: 5544 2133 2037 455
Fragments arranged by size:
5544 2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 7600 7848 8550 10046 7600
Size: 248 702 1496 7723
Fragments arranged by size:
7723 1496 702 248
BsaWI w'CCGG_w
Cuts at: 126 338 1174 4664 7358 7879 8557 8831 8978
Size: 212 836 3490 2694 521 678 274 147
Cuts at: 8978 9389 126
Size: 411 906
Fragments arranged by size:
3490 2694 906 836 678 521 411 274
212 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1031 1061 1130 1160 1859 1889 2057 2087 2505
Size: 30 69 30 699 30 168 30 418
Cuts at: 2505 2535 2845 2875 3936 3966 5613 5643 5751
Size: 30 310 30 1061 30 1647 30 108
Cuts at: 5751 5781 6155 6185 6663 6693 1031
Size: 30 374 30 478 30 4507
Fragments arranged by size:
4507 1647 1061 699 478 418 374 310
168 108 69 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 248 502 1589 2330 2684 3005 3297 3509 3751
Size: 254 1087 741 354 321 292 212 242
Cuts at: 3751 4598 4779 5003 6446 248
Size: 847 181 224 1443 3971
Fragments arranged by size:
3971 1443 1087 847 741 354 321 292
254 242 224 212 181
BseYI C'CCAG_C
Cuts at: 355 5504 5855 6595 7594 8352 8929 9425 355
Size: 5149 351 740 999 758 577 496 1099
Fragments arranged by size:
5149 1099 999 758 740 577 496 351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 743 2144 2792 3503 5405 6506 6635 611
Size: 132 1401 648 711 1902 1101 129 4145
Fragments arranged by size:
4145 1902 1401 1101 711 648 132 129
BsiEI CG_ry'CG
Cuts at: 104 7711 7757 8965 104
Size: 7607 46 1208 1308
Fragments arranged by size:
7607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 243 1166 2687 2888 3387 4626 6438 6552 7183
Size: 923 1521 201 499 1239 1812 114 631
Cuts at: 7183 7961 8151 8943 243
Size: 778 190 792 1469
Fragments arranged by size:
1812 1521 1469 1239 923 792 778 631
499 201 190 114
BsmI GAATG_Cn'
Cuts at: 1293 6972 1293
Size: 5679 4490
BsmBI CGTCTCn'nnnn_
Cuts at: 260 260
Size: 10169
Bsp1286I G_dGCh'C
Cuts at: 166 193 243 1166 1320 1482 2340 2687 2888
Size: 27 50 923 154 162 858 347 201
Cuts at: 2888 3079 3387 4626 5155 6096 6113 6423 6438
Size: 191 308 1239 529 941 17 310 15
Cuts at: 6438 6552 7128 7183 7794 7887 7961 8151 8213
Size: 114 576 55 611 93 74 190 62
Cuts at: 8213 8943 166
Size: 730 1392
Fragments arranged by size:
1392 1239 941 923 858 730 611 576
529 347 310 308 201 191 190 162
154 114 93 74 62 55 50 27
17 15
BspEI T'CCGG_A
Cuts at: 9389 9389
Size: 10169
BspHI T'CATG_A
Cuts at: 2601 9345 2601
Size: 6744 3425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 293 2409 3469 4000 5230 5581 6306 7219 7433
Size: 2116 1060 531 1230 351 725 913 214
Cuts at: 7433 7735 8116 293
Size: 302 381 2346
Fragments arranged by size:
2346 2116 1230 1060 913 725 531 381
351 302 214
BsrBI CCG'CTC
Cuts at: 50 8461 8515 9999 10164 50
Size: 8411 54 1484 165 55
Fragments arranged by size:
8411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 977 2468 6606 6857 6977 8081 977
Size: 1491 4138 251 120 1104 3065
Fragments arranged by size:
4138 3065 1491 1104 251 120
BsrFI r'CCGG_y
Cuts at: 8167 8348 8557 8167
Size: 181 209 9779
Fragments arranged by size:
9779 209 181
BsrGI T'GTAC_A
Cuts at: 4423 4423
Size: 10169
BssSI C'ACGA_G
Cuts at: 260 2342 2638 8440 8798 260
Size: 2082 296 5802 358 1631
Fragments arranged by size:
5802 2082 1631 358 296
BstAPI GCAn_nnn'nTGC
Cuts at: 976 7546 976
Size: 6570 3599
BstBI TT'CG_AA
Cuts at: 8530 8530
Size: 10169
BstYI r'GATC_y
Cuts at: 1 609 696 1971 2172 4737 5060 5692 6166
Size: 608 87 1275 201 2565 323 632 474
Cuts at: 6166 6237 7194 7661 7684 8019 8265 9277 9363
Size: 71 957 467 23 335 246 1012 86
Cuts at: 9363 9375 9721 1
Size: 12 346 449
Fragments arranged by size:
2565 1275 1012 957 632 608 474 467
449 346 335 323 246 201 87 86
71 23 12
Bsu36I CC'TnA_GG
Cuts at: 598 2381 6312 598
Size: 1783 3931 4455
Fragments arranged by size:
4455 3931 1783
BtgI C'CryG_G
Cuts at: 107 197 671 2366 5399 7082 107
Size: 90 474 1695 3033 1683 3194
Fragments arranged by size:
3194 3033 1695 1683 474 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4238 8119 8300 8463 4238
Size: 3881 181 163 5944
Fragments arranged by size:
5944 3881 181 163
BtsI GCAGTG_nn'
Cuts at: 1412 2147 2359 2656 3113 4829 5223 5417 7412
Size: 735 212 297 457 1716 394 194 1995
Cuts at: 7412 1412
Size: 4169
Fragments arranged by size:
4169 1995 1716 735 457 394 297 212
194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2699 2734 2801 2836 5057 5092 5546 5581 7090
Size: 35 67 35 2221 35 454 35 1509
Cuts at: 7090 7125 2699
Size: 35 5743
Fragments arranged by size:
5743 2221 1509 454 67 35 35 35
35 35
DraI TTT'AAA
Cuts at: 1308 2330 7168 1308
Size: 1022 4838 4309
Fragments arranged by size:
4838 4309 1022
DraIII CAC_nnn'GTG
Cuts at: 5190 6037 5190
Size: 847 9322
DrdI GACnn_nn'nnGTC
Cuts at: 7875 8733 7875
Size: 858 9311
EaeI y'GGCC_r
Cuts at: 1440 2229 2634 7754 7928 8319 8346 9691 1440
Size: 789 405 5120 174 391 27 1345 1918
Fragments arranged by size:
5120 1918 1345 789 405 391 174 27
EagI C'GGCC_G
Cuts at: 7754 7754
Size: 10169
EarI CTCTTCn'nnn_
Cuts at: 458 479 1583 2426 2445 3808 4334 4575 5000
Size: 21 1104 843 19 1363 526 241 425
Cuts at: 5000 5726 6006 8192 8402 458
Size: 726 280 2186 210 2225
Fragments arranged by size:
2225 2186 1363 1104 843 726 526 425
280 241 210 21 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 220 434 8377 8687 8833 9979 220
Size: 214 7943 310 146 1146 410
Fragments arranged by size:
7943 1146 410 310 214 146
EcoICRI GAG'CTC
Cuts at: 241 3385 7181 241
Size: 3144 3796 3229
Fragments arranged by size:
3796 3229 3144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 722 1157 1942 2009 2528 3170 3281 3667
Size: 409 435 785 67 519 642 111 386
Cuts at: 3667 3725 3956 4070 4679 5707 5774 5918 6254
Size: 58 231 114 609 1028 67 144 336
Cuts at: 6254 6965 7028 7994 8426 9173 9441 313
Size: 711 63 966 432 747 268 1041
Fragments arranged by size:
1041 1028 966 785 747 711 642 609
519 435 432 409 386 336 268 231
144 114 111 67 67 63 58
EcoNI CCTnn'n_nnAGG
Cuts at: 1588 1588
Size: 10169
EcoO109I rG'GnC_Cy
Cuts at: 190 6110 7012 7308 190
Size: 5920 902 296 3051
Fragments arranged by size:
5920 3051 902 296
EcoRI G'AATT_C
Cuts at: 5232 5925 6048 7173 5232
Size: 693 123 1125 8228
Fragments arranged by size:
8228 1125 693 123
EcoRV GAT'ATC
Cuts at: 4565 4565
Size: 10169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 585 1182 2021 3931 5345 8188 585
Size: 597 839 1910 1414 2843 2566
Fragments arranged by size:
2843 2566 1910 1414 839 597
FspI TGC'GCA
Cuts at: 7950 7950
Size: 10169
HaeII r_GCGC'y
Cuts at: 2614 7603 7851 8873 9852 2614
Size: 4989 248 1022 979 2931
Fragments arranged by size:
4989 2931 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 490 522 594 626 1031 1063 1667 1699 2505
Size: 32 72 32 405 32 604 32 806
Cuts at: 2505 2537 3061 3093 3504 3536 3936 3968 4248
Size: 32 524 32 411 32 400 32 280
Cuts at: 4248 4280 4333 4365 4722 4754 5530 5562 5751
Size: 32 53 32 357 32 776 32 189
Cuts at: 5751 5783 6780 6812 8011 8041 8043 8073 490
Size: 32 997 32 1199 30 2 30 2586
Fragments arranged by size:
2586 1199 997 806 776 604 524 411
405 400 357 280 189 72 53 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 719 2309 4469 6188 6488 7208 719
Size: 1590 2160 1719 300 720 3680
Fragments arranged by size:
3680 2160 1719 1590 720 300
HindIII A'AGCT_T
Cuts at: 635 3913 635
Size: 3278 6891
HpaI GTT'AAC
Cuts at: 2309 4469 2309
Size: 2160 8009
KasI G'GCGC_C
Cuts at: 7599 7847 7599
Size: 248 9921
KpnI G_GTAC'C
Cuts at: 7189 7189
Size: 10169
MfeI C'AATT_G
Cuts at: 4714 4714
Size: 10169
MluI A'CGCG_T
Cuts at: 9602 9602
Size: 10169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 722 785 2542 3751 8840 9024 722
Size: 63 1757 1209 5089 184 1867
Fragments arranged by size:
5089 1867 1757 1209 184 63
MscI TGG'CCA
Cuts at: 1442 2231 2636 7930 1442
Size: 789 405 5294 3681
Fragments arranged by size:
5294 3681 789 405
MslI CAynn'nnrTG
Cuts at: 980 1298 1937 2237 2741 2891 3392 3452 5359
Size: 318 639 300 504 150 501 60 1907
Cuts at: 5359 6902 8158 8285 980
Size: 1543 1256 127 2864
Fragments arranged by size:
2864 1907 1543 1256 639 504 501 318
300 150 127 60
MspA1I CmG'CkG
Cuts at: 199 392 1475 2731 3049 4217 4276 6445 7280
Size: 193 1083 1256 318 1168 59 2169 835
Cuts at: 7280 7594 7954 8967 9212 199
Size: 314 360 1013 245 1156
Fragments arranged by size:
2169 1256 1168 1156 1083 1013 835 360
318 314 245 193 59
NaeI GCC'GGC
Cuts at: 8350 8350
Size: 10169
NarI GG'CG_CC
Cuts at: 7600 7848 7600
Size: 248 9921
NcoI C'CATG_G
Cuts at: 107 2366 107
Size: 2259 7910
NdeI CA'TA_TG
Cuts at: 1125 1914 6339 7148 1125
Size: 789 4425 809 4146
Fragments arranged by size:
4425 4146 809 789
NgoMIV G'CCGG_C
Cuts at: 8348 8348
Size: 10169
NheI G'CTAG_C
Cuts at: 1068 1579 3429 1068
Size: 511 1850 7808
Fragments arranged by size:
7808 1850 511
NsiI A_TGCA'T
Cuts at: 977 977
Size: 10169
NspI r_CATG'y
Cuts at: 979 1048 1246 1494 1535 1546 5032 6907 7222
Size: 69 198 248 41 11 3486 1875 315
Cuts at: 7222 7722 8629 979
Size: 500 907 2519
Fragments arranged by size:
3486 2519 1875 907 500 315 248 198
69 41 11
PasI CC'CwG_GG
Cuts at: 6784 7121 6784
Size: 337 9832
PciI A'CATG_T
Cuts at: 1044 1242 8625 1044
Size: 198 7383 2588
Fragments arranged by size:
7383 2588 198
PfoI T'CCnGG_A
Cuts at: 5688 5688
Size: 10169
PmeI GTTT'AAAC
Cuts at: 7168 7168
Size: 10169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1859 1891 6663 6695 9308 9340 1859
Size: 32 4772 32 2613 32 2688
Fragments arranged by size:
4772 2688 2613 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 7012 7012
Size: 10169
PshAI GACnn'nnGTC
Cuts at: 6816 6816
Size: 10169
PspOMI G'GGCC_C
Cuts at: 189 6109 189
Size: 5920 4249
PspXI vC'TCGA_Gb
Cuts at: 2867 2867
Size: 10169
PstI C_TGCA'G
Cuts at: 150 654 1866 4222 6915 7041 7216 7901 150
Size: 504 1212 2356 2693 126 175 685 2418
Fragments arranged by size:
2693 2418 2356 1212 685 504 175 126
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10169
PvuII CAG'CTG
Cuts at: 2731 3049 4217 4276 6445 7594 7954 2731
Size: 318 1168 59 2169 1149 360 4946
Fragments arranged by size:
4946 2169 1168 1149 360 318 59
RsrII CG'GwC_CG
Cuts at: 8364 8364
Size: 10169
SacI G_AGCT'C
Cuts at: 243 3387 7183 243
Size: 3144 3796 3229
Fragments arranged by size:
3796 3229 3144
SacII CC_GC'GG
Cuts at: 200 200
Size: 10169
SalI G'TCGA_C
Cuts at: 7206 7206
Size: 10169
SapI GCTCTTCn'nnn_
Cuts at: 2445 8192 8402 2445
Size: 5747 210 4212
Fragments arranged by size:
5747 4212 210
SbfI CC_TGCA'GG
Cuts at: 6915 7216 6915
Size: 301 9868
ScaI AGT'ACT
Cuts at: 707 9686 707
Size: 8979 1190
SfcI C'TryA_G
Cuts at: 33 146 650 1862 4218 4871 6911 7037 7212
Size: 113 504 1212 2356 653 2040 126 175
Cuts at: 7212 7897 8890 9081 33
Size: 685 993 191 1121
Fragments arranged by size:
2356 2040 1212 1121 993 685 653 504
191 175 126 113
SfoI GGC'GCC
Cuts at: 7601 7849 7601
Size: 248 9921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10169
SmaI CCC'GGG
Cuts at: 7191 7191
Size: 10169
SmlI C'TyrA_G
Cuts at: 1631 1706 1823 2867 3309 3899 6024 7324 8239
Size: 75 117 1044 442 590 2125 1300 915
Cuts at: 8239 8731 8993 1631
Size: 492 262 2807
Fragments arranged by size:
2807 2125 1300 1044 915 590 492 442
262 117 75
SnaBI TAC'GTA
Cuts at: 3400 3400
Size: 10169
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10169
SphI G_CATG'C
Cuts at: 979 7222 7722 979
Size: 6243 500 3426
Fragments arranged by size:
6243 3426 500
SspI AAT'ATT
Cuts at: 2756 2957 2756
Size: 201 9968
StuI AGG'CCT
Cuts at: 4977 5145 4977
Size: 168 10001
StyI C'CwwG_G
Cuts at: 107 2366 2801 2819 3138 5071 6426 7303 7657
Size: 2259 435 18 319 1933 1355 877 354
Cuts at: 7657 107
Size: 2619
Fragments arranged by size:
2619 2259 1933 1355 877 435 354 319
18
SwaI ATTT'AAAT
Cuts at: 2330 2330
Size: 10169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5439 6828 7752 7885 8344 8553 5439
Size: 1389 924 133 459 209 7055
Fragments arranged by size:
7055 1389 924 459 209 133
TatI w'GTAC_w
Cuts at: 705 2038 2106 3678 4423 6028 6054 6865 9684
Size: 1333 68 1572 745 1605 26 811 2819
Cuts at: 9684 705
Size: 1190
Fragments arranged by size:
2819 1605 1572 1333 1190 811 745 68
26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 2406 2438 5112 5144 5318 5350 5613
Size: 32 317 32 2674 32 174 32 263
Cuts at: 5613 5645 9686 9718 2057
Size: 32 4041 32 2508
Fragments arranged by size:
4041 2674 2508 317 263 174 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 723 7966 723
Size: 7243 2926
XbaI T'CTAG_A
Cuts at: 2986 5521 7200 2986
Size: 2535 1679 5955
Fragments arranged by size:
5955 2535 1679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1201 7664 1201
Size: 6463 3706
XhoI C'TCGA_G
Cuts at: 1631 2867 1631
Size: 1236 8933
XmaI C'CCGG_G
Cuts at: 7189 7189
Size: 10169
XmnI GAAnn'nnTTC
Cuts at: 707 4094 4763 707
Size: 3387 669 6113
Fragments arranged by size:
6113 3387 669
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AscI AvrII BaeI BglI BmgBI
BsiWI BssHII BstEII BstXI BstZ17I ClaI FseI FspAI
NotI NruI PacI PflMI PmlI PsiI PsrI SanDI
SexAI SfiI SgrAI SrfI ZraI