Restriction map for
pF1KB7324
(Circular) (Six-base) MAPSORT of: pF1KB7324.seq Check: 5115 from: 1 to: 6950
pF1KB7324 6950 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2589 3473 3509 2589
Size: 884 36 6030
Fragments arranged by size:
6030 884 36
AccI GT'mk_AC
Cuts at: 3988 3988
Size: 6950
Acc65I G'GTAC_C
Cuts at: 3966 3966
Size: 6950
AclI AA'CG_TT
Cuts at: 6790 6790
Size: 6950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 1236 2061 2296 3108 3499 4775 5207 5954
Size: 879 825 235 812 391 1276 432 747
Cuts at: 5954 6222 357
Size: 268 1085
Fragments arranged by size:
1276 1085 879 825 812 747 432 391
268 235
AfeI AGC'GCT
Cuts at: 1736 3779 1736
Size: 2043 4907
AflIII A'CryG_T
Cuts at: 2333 3200 4302 5406 6383 2333
Size: 867 1102 1104 977 2900
Fragments arranged by size:
2900 1104 1102 977 867
AgeI A'CCGG_T
Cuts at: 5338 5338
Size: 6950
AleI CACnn'nnGTG
Cuts at: 427 2836 2857 427
Size: 2409 21 4520
Fragments arranged by size:
4520 2409 21
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2551 2875 2551
Size: 324 6626
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 457 489 1652 1684 1873 1905 1934 1966 1995
Size: 32 1163 32 189 32 29 32 29
Cuts at: 1995 2027 457
Size: 32 5380
Fragments arranged by size:
5380 1163 189 32 32 32 32 32
29 29
AlwNI CAG_nnn'CTG
Cuts at: 1173 1475 1576 2274 2661 3822 4255 5822 1173
Size: 302 101 698 387 1161 433 1567 2301
Fragments arranged by size:
2301 1567 1161 698 433 387 302 101
ApaI G_GGCC'C
Cuts at: 247 821 1504 2245 3346 3788 247
Size: 574 683 741 1101 442 3409
Fragments arranged by size:
3409 1101 741 683 574 442
ApaLI G'TGCA_C
Cuts at: 197 2337 2389 3460 5720 197
Size: 2140 52 1071 2260 1427
Fragments arranged by size:
2260 2140 1427 1071 52
ApoI r'AATT_y
Cuts at: 2439 3954 6303 6559 2439
Size: 1515 2349 256 2830
Fragments arranged by size:
2830 2349 1515 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6950
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6950
AvaI C'yCGr_G
Cuts at: 266 425 788 948 1145 1758 2238 2908 2935
Size: 159 363 160 197 613 480 670 27
Cuts at: 2935 3565 3715 3781 3970 266
Size: 630 150 66 189 3246
Fragments arranged by size:
3246 670 630 613 480 363 197 189
160 159 150 66 27
AvrII C'CTAG_G
Cuts at: 582 582
Size: 6950
BamHI G'GATC_C
Cuts at: 2951 3975 2951
Size: 1024 5926
BanI G'GyrC_C
Cuts at: 682 757 904 1149 1457 2794 2851 2870 2962
Size: 75 147 245 308 1337 57 19 92
Cuts at: 2962 2989 3139 3478 3509 3548 3889 3966 4380
Size: 27 150 339 31 39 341 77 414
Cuts at: 4380 4628 4663 682
Size: 248 35 2969
Fragments arranged by size:
2969 1337 414 341 339 308 248 245
150 147 92 77 75 57 39 35
31 27 19
BanII G_rGCy'C
Cuts at: 247 356 515 787 821 949 1036 1114 1504
Size: 109 159 272 34 128 87 78 390
Cuts at: 1504 1871 2237 2245 2786 3346 3566 3736 3788
Size: 367 366 8 541 560 220 170 52
Cuts at: 3788 3964 4994 247
Size: 176 1030 2203
Fragments arranged by size:
2203 1030 560 541 390 367 366 272
220 176 170 159 128 109 87 78
52 34 8
BbeI G_GCGC'C
Cuts at: 2798 2874 2993 3143 3482 4384 4632 2798
Size: 76 119 150 339 902 248 5116
Fragments arranged by size:
5116 902 339 248 150 119 76
BbsI GAAGACnn'nnnn_
Cuts at: 363 2284 3114 363
Size: 1921 830 4199
Fragments arranged by size:
4199 1921 830
BbvCI CC'TCA_GC
Cuts at: 1894 1955 2016 1894
Size: 61 61 6828
Fragments arranged by size:
6828 61 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6916
BciVI GTATCCnnnnn_n'
Cuts at: 1675 4848 5609 6950 1675
Size: 3173 761 1341 1675
Fragments arranged by size:
3173 1675 1341 761
BclI T'GATC_A
Cuts at: 4470 4470
Size: 6950
BglI GCCn_nnn'nGGC
Cuts at: 212 791 883 1489 1508 2420 3073 3082 3137
Size: 579 92 606 19 912 653 9 55
Cuts at: 3137 3268 3518 3599 212
Size: 131 250 81 3563
Fragments arranged by size:
3563 912 653 606 579 250 131 92
81 55 19 9
BglII A'GATC_T
Cuts at: 4465 6502 4465
Size: 2037 4913
BlpI GC'TnA_GC
Cuts at: 1525 4062 1525
Size: 2537 4413
Bme1580I G_kGCm'C
Cuts at: 201 247 762 821 1152 1504 2245 2341 2393
Size: 46 515 59 331 352 741 96 52
Cuts at: 2393 2854 3346 3464 3491 3788 4575 4668 5724
Size: 461 492 118 27 297 787 93 1056
Cuts at: 5724 201
Size: 1427
Fragments arranged by size:
1427 1056 787 741 515 492 461 352
331 297 118 96 93 59 52 46
27
BmgBI CAC'GTC
Cuts at: 3201 3201
Size: 6950
BmrI ACTGGGnnnn_n'
Cuts at: 3319 4162 4243 4578 6722 3319
Size: 843 81 335 2144 3547
Fragments arranged by size:
3547 2144 843 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 416 5317 416
Size: 4901 2049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 819 865 915 1059 1122 769
Size: 50 46 50 144 63 6597
Fragments arranged by size:
6597 144 63 50 50 46
Bpu10I CC'TnA_GC
Cuts at: 1894 1955 2016 2665 1894
Size: 61 61 649 6179
Fragments arranged by size:
6179 649 61 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4126 5005 5497 5795 4126
Size: 879 492 298 5281
Fragments arranged by size:
5281 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1342 6654 1342
Size: 5312 1638
BsaAI yAC'GTr
Cuts at: 1704 2859 3071 3459 4303 1704
Size: 1155 212 388 844 4351
Fragments arranged by size:
4351 1155 844 388 212
BsaBI GATnn'nnATC
Cuts at: 6 4464 6501 6
Size: 4458 2037 455
Fragments arranged by size:
4458 2037 455
BsaHI Gr'CG_yC
Cuts at: 558 891 2795 2871 2990 3140 3242 3260 3479
Size: 333 1904 76 119 150 102 18 219
Cuts at: 3479 4381 4629 5331 6827 558
Size: 902 248 702 1496 681
Fragments arranged by size:
1904 1496 902 702 681 333 248 219
150 119 102 76 18
BsaWI w'CCGG_w
Cuts at: 4139 4660 5338 5612 5759 6170 4139
Size: 521 678 274 147 411 4919
Fragments arranged by size:
4919 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1557 1587 1652 1682 3553 3583 1557
Size: 30 65 30 1871 30 4924
Fragments arranged by size:
4924 1871 65 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 1012 1324 1552 2926 3487 3574 523
Size: 489 312 228 1374 561 87 3899
Fragments arranged by size:
3899 1374 561 489 312 228 87
BseYI C'CCAG_C
Cuts at: 253 514 694 753 770 840 1328 1773 2785
Size: 261 180 59 17 70 488 445 1012
Cuts at: 2785 2808 3276 3552 3634 3710 3838 4375 5133
Size: 23 468 276 82 76 128 537 758
Cuts at: 5133 5710 6206 253
Size: 577 496 997
Fragments arranged by size:
1012 997 758 577 537 496 488 468
445 276 261 180 128 82 76 70
59 23 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 840 2019 2302 2521 2881 3280 3487 3663
Size: 295 1179 283 219 360 399 207 176
Cuts at: 3663 545
Size: 3832
Fragments arranged by size:
3832 1179 399 360 295 283 219 207
176
BsiEI CG_ry'CG
Cuts at: 104 344 522 827 2345 2946 4492 4538 5746
Size: 240 178 305 1518 601 1546 46 1208
Cuts at: 5746 104
Size: 1308
Fragments arranged by size:
1546 1518 1308 1208 601 305 240 178
46
BsiHKAI G_wGCw'C
Cuts at: 201 1180 2145 2237 2288 2341 2393 3014 3173
Size: 979 965 92 51 53 52 621 159
Cuts at: 3173 3464 3964 4742 4932 5724 201
Size: 291 500 778 190 792 1427
Fragments arranged by size:
1427 979 965 792 778 621 500 291
190 159 92 53 52 51
BsmI GAATG_Cn'
Cuts at: 2089 2089
Size: 6950
BsmBI CGTCTCn'nnnn_
Cuts at: 423 423
Size: 6950
Bsp1286I G_dGCh'C
Cuts at: 201 247 356 515 762 787 821 949 1036
Size: 46 109 159 247 25 34 128 87
Cuts at: 1036 1114 1152 1180 1504 1871 2145 2237 2245
Size: 78 38 28 324 367 274 92 8
Cuts at: 2245 2288 2341 2393 2786 2854 3014 3173 3346
Size: 43 53 52 393 68 160 159 173
Cuts at: 3346 3464 3491 3566 3736 3788 3964 4575 4668
Size: 118 27 75 170 52 176 611 93
Cuts at: 4668 4742 4932 4994 5724 201
Size: 74 190 62 730 1427
Fragments arranged by size:
1427 730 611 393 367 324 274 247
190 176 173 170 160 159 159 128
118 109 93 92 87 78 75 74
68 62 53 52 52 46 43 38
34 28 27 25 8
BspEI T'CCGG_A
Cuts at: 6170 6170
Size: 6950
BspHI T'CATG_A
Cuts at: 6126 6126
Size: 6950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 506 2589 2876 3371 3473 3509 4000 4214 4516
Size: 2083 287 495 102 36 491 214 302
Cuts at: 4516 4897 506
Size: 381 2559
Fragments arranged by size:
2559 2083 495 491 381 302 287 214
102 36
BsrBI CCG'CTC
Cuts at: 50 5242 5296 6780 6945 50
Size: 5192 54 1484 165 55
Fragments arranged by size:
5192 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4862 4862
Size: 6950
BsrFI r'CCGG_y
Cuts at: 284 2423 2479 3399 3545 3901 4948 5129 5338
Size: 2139 56 920 146 356 1047 181 209
Cuts at: 5338 284
Size: 1896
Fragments arranged by size:
2139 1896 1047 920 356 209 181 146
56
BssHII G'CGCG_C
Cuts at: 3033 3033
Size: 6950
BssSI C'ACGA_G
Cuts at: 780 1195 5221 5579 780
Size: 415 4026 358 2151
Fragments arranged by size:
4026 2151 415 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1576 2661 2980 4327 1576
Size: 1085 319 1347 4199
Fragments arranged by size:
4199 1347 1085 319
BstBI TT'CG_AA
Cuts at: 5311 5311
Size: 6950
BstEII G'GTnAC_C
Cuts at: 1852 2601 2829 3705 1852
Size: 749 228 876 5097
Fragments arranged by size:
5097 876 749 228
BstXI CCAn_nnnn'nTGG
Cuts at: 429 939 1360 1810 3642 429
Size: 510 421 450 1832 3737
Fragments arranged by size:
3737 1832 510 450 421
BstYI r'GATC_y
Cuts at: 1 2271 2951 3975 4442 4465 4800 5046 6058
Size: 2270 680 1024 467 23 335 246 1012
Cuts at: 6058 6144 6156 6502 1
Size: 86 12 346 449
Fragments arranged by size:
2270 1024 1012 680 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1535 2217 1535
Size: 682 6268
BtgI C'CryG_G
Cuts at: 117 821 860 1353 2154 2324 2946 3319 3701
Size: 704 39 493 801 170 622 373 382
Cuts at: 3701 117
Size: 3366
Fragments arranged by size:
3366 801 704 622 493 382 373 170
39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4900 5081 5244 4900
Size: 181 163 6606
Fragments arranged by size:
6606 181 163
BtsI GCAGTG_nn'
Cuts at: 201 2126 3180 3214 3426 3729 4193 201
Size: 1925 1054 34 212 303 464 2958
Fragments arranged by size:
2958 1925 1054 464 303 212 34
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2192 2227 3370 3405 2192
Size: 35 1143 35 5737
Fragments arranged by size:
5737 1143 35 35
DraI TTT'AAA
Cuts at: 3949 3949
Size: 6950
DraIII CAC_nnn'GTG
Cuts at: 2325 3410 2325
Size: 1085 5865
DrdI GACnn_nn'nnGTC
Cuts at: 225 4656 5514 225
Size: 4431 858 1661
Fragments arranged by size:
4431 1661 858
EaeI y'GGCC_r
Cuts at: 341 519 824 1450 1481 1593 1800 2342 2943
Size: 178 305 626 31 112 207 542 601
Cuts at: 2943 2956 3065 3401 3789 4535 4709 5100 5127
Size: 13 109 336 388 746 174 391 27
Cuts at: 5127 6472 341
Size: 1345 819
Fragments arranged by size:
1345 819 746 626 601 542 391 388
336 305 207 178 174 112 109 31
27 13
EagI C'GGCC_G
Cuts at: 341 519 824 2342 2943 4535 341
Size: 178 305 1518 601 1592 2756
Fragments arranged by size:
2756 1592 1518 601 305 178
EarI CTCTTCn'nnn_
Cuts at: 407 1630 2099 3169 4973 5183 407
Size: 1223 469 1070 1804 210 2174
Fragments arranged by size:
2174 1804 1223 1070 469 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1660 3460 5158 5468 5614 6760 1660
Size: 1800 1698 310 146 1146 1850
Fragments arranged by size:
1850 1800 1698 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2235 3962 2235
Size: 1727 5223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 769 819 865 915 1059 1122 1236 2061
Size: 412 50 46 50 144 63 114 825
Cuts at: 2061 2296 3108 3499 4775 5207 5954 6222 357
Size: 235 812 391 1276 432 747 268 1085
Fragments arranged by size:
1276 1085 825 812 747 432 412 391
268 235 144 114 63 50 50 46
EcoNI CCTnn'n_nnAGG
Cuts at: 1089 1452 1534 2515 3469 1089
Size: 363 82 981 954 4570
Fragments arranged by size:
4570 981 954 363 82
EcoO109I rG'GnC_Cy
Cuts at: 243 297 481 1094 1425 1472 1584 1755 1764
Size: 54 184 613 331 47 112 171 9
Cuts at: 1764 2181 2613 2691 3343 3919 4089 243
Size: 417 432 78 652 576 170 3104
Fragments arranged by size:
3104 652 613 576 432 417 331 184
171 170 112 78 54 47 9
EcoRI G'AATT_C
Cuts at: 3954 3954
Size: 6950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4969 4969
Size: 6950
FspI TGC'GCA
Cuts at: 2899 4731 2899
Size: 1832 5118
HaeII r_GCGC'y
Cuts at: 1134 1713 1738 2402 2798 2874 2993 3088 3143
Size: 579 25 664 396 76 119 95 55
Cuts at: 3143 3482 3781 4384 4632 5654 6633 1134
Size: 339 299 603 248 1022 979 1451
Fragments arranged by size:
1451 1022 979 664 603 579 396 339
299 248 119 95 76 55 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1303 1335 2256 2288 4792 4822 4824 4854 1303
Size: 32 921 32 2504 30 2 30 3399
Fragments arranged by size:
3399 2504 921 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3989 3989
Size: 6950
KasI G'GCGC_C
Cuts at: 2794 2870 2989 3139 3478 4380 4628 2794
Size: 76 119 150 339 902 248 5116
Fragments arranged by size:
5116 902 339 248 150 119 76
KpnI G_GTAC'C
Cuts at: 3970 3970
Size: 6950
MluI A'CGCG_T
Cuts at: 2333 6383 2333
Size: 4050 2900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1883 1944 2005 5621 5805 1883
Size: 61 61 3616 184 3028
Fragments arranged by size:
3616 3028 184 61 61
MscI TGG'CCA
Cuts at: 4711 4711
Size: 6950
MslI CAynn'nnrTG
Cuts at: 363 427 1808 2836 2857 4939 5066 363
Size: 64 1381 1028 21 2082 127 2247
Fragments arranged by size:
2247 2082 1381 1028 127 64 21
MspA1I CmG'CkG
Cuts at: 253 518 541 1332 1517 1773 1788 2085 2247
Size: 265 23 791 185 256 15 297 162
Cuts at: 2247 2684 2865 2919 3030 3128 3749 4061 4375
Size: 437 181 54 111 98 621 312 314
Cuts at: 4375 4735 5748 5993 253
Size: 360 1013 245 1210
Fragments arranged by size:
1210 1013 791 621 437 360 314 312
297 265 256 245 185 181 162 111
98 54 23 15
NaeI GCC'GGC
Cuts at: 2425 2481 3547 5131 2425
Size: 56 1066 1584 4244
Fragments arranged by size:
4244 1584 1066 56
NarI GG'CG_CC
Cuts at: 2795 2871 2990 3140 3479 4381 4629 2795
Size: 76 119 150 339 902 248 5116
Fragments arranged by size:
5116 902 339 248 150 119 76
NcoI C'CATG_G
Cuts at: 860 3701 860
Size: 2841 4109
NgoMIV G'CCGG_C
Cuts at: 2423 2479 3545 5129 2423
Size: 56 1066 1584 4244
Fragments arranged by size:
4244 1584 1066 56
NruI TCG'CGA
Cuts at: 1271 1271
Size: 6950
NspI r_CATG'y
Cuts at: 3224 4003 4503 5410 3224
Size: 779 500 907 4764
Fragments arranged by size:
4764 907 779 500
PasI CC'CwG_GG
Cuts at: 762 896 2305 2878 3156 3684 762
Size: 134 1409 573 278 528 4028
Fragments arranged by size:
4028 1409 573 528 278 134
PciI A'CATG_T
Cuts at: 5406 5406
Size: 6950
PflMI CCAn_nnn'nTGG
Cuts at: 253 2523 2564 3111 253
Size: 2270 41 547 4092
Fragments arranged by size:
4092 2270 547 41
PfoI T'CCnGG_A
Cuts at: 318 714 1036 3110 318
Size: 396 322 2074 4158
Fragments arranged by size:
4158 2074 396 322
PmeI GTTT'AAAC
Cuts at: 3949 3949
Size: 6950
PmlI CAC'GTG
Cuts at: 3071 3459 3071
Size: 388 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1652 1684 2998 3030 6089 6121 1652
Size: 32 1314 32 3059 32 2481
Fragments arranged by size:
3059 2481 1314 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 481 1094 1584 1755 1764 2691 3919 481
Size: 613 490 171 9 927 1228 3512
Fragments arranged by size:
3512 1228 927 613 490 171 9
PspOMI G'GGCC_C
Cuts at: 243 817 1500 2241 3342 3784 243
Size: 574 683 741 1101 442 3409
Fragments arranged by size:
3409 1101 741 683 574 442
PstI C_TGCA'G
Cuts at: 1516 1772 1913 1974 2035 2136 3005 3368 3470
Size: 256 141 61 61 101 869 363 102
Cuts at: 3470 3997 4682 1516
Size: 527 685 3784
Fragments arranged by size:
3784 869 685 527 363 256 141 102
101 61 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6950
PvuII CAG'CTG
Cuts at: 541 1773 2085 2865 3030 3128 3749 4375 4735
Size: 1232 312 780 165 98 621 626 360
Cuts at: 4735 541
Size: 2756
Fragments arranged by size:
2756 1232 780 626 621 360 312 165
98
RsrII CG'GwC_CG
Cuts at: 5145 5145
Size: 6950
SacI G_AGCT'C
Cuts at: 2237 3964 2237
Size: 1727 5223
SalI G'TCGA_C
Cuts at: 3987 3987
Size: 6950
SanDI GG'GwC_CC
Cuts at: 1584 1755 2691 3919 1584
Size: 171 936 1228 4615
Fragments arranged by size:
4615 1228 936 171
SapI GCTCTTCn'nnn_
Cuts at: 407 1630 4973 5183 407
Size: 1223 3343 210 2174
Fragments arranged by size:
3343 2174 1223 210
SbfI CC_TGCA'GG
Cuts at: 3368 3470 3997 3368
Size: 102 527 6321
Fragments arranged by size:
6321 527 102
ScaI AGT'ACT
Cuts at: 6467 6467
Size: 6950
SfcI C'TryA_G
Cuts at: 33 1512 1768 1909 1970 2031 2132 3001 3364
Size: 1479 256 141 61 61 101 869 363
Cuts at: 3364 3466 3744 3993 4678 5671 5862 33
Size: 102 278 249 685 993 191 1121
Fragments arranged by size:
1479 1121 993 869 685 363 278 256
249 191 141 102 101 61 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 883 1489 3073 883
Size: 606 1584 4760
Fragments arranged by size:
4760 1584 606
SfoI GGC'GCC
Cuts at: 2796 2872 2991 3141 3480 4382 4630 2796
Size: 76 119 150 339 902 248 5116
Fragments arranged by size:
5116 902 339 248 150 119 76
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6950
SgrAI Cr'CCGG_yG
Cuts at: 2479 2479
Size: 6950
SmaI CCC'GGG
Cuts at: 268 790 1147 1760 3972 268
Size: 522 357 613 2212 3246
Fragments arranged by size:
3246 2212 613 522 357
SmlI C'TyrA_G
Cuts at: 4105 5020 5512 5774 4105
Size: 915 492 262 5281
Fragments arranged by size:
5281 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6950
SphI G_CATG'C
Cuts at: 3224 4003 4503 3224
Size: 779 500 5671
Fragments arranged by size:
5671 779 500
StuI AGG'CCT
Cuts at: 1289 1342 1447 1492 3878 1289
Size: 53 105 45 2386 4361
Fragments arranged by size:
4361 2386 105 53 45
StyI C'CwwG_G
Cuts at: 582 860 1262 1304 1359 1484 3701 3857 4084
Size: 278 402 42 55 125 2217 156 227
Cuts at: 4084 4438 582
Size: 354 3094
Fragments arranged by size:
3094 2217 402 354 278 227 156 125
55 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 689 745 1066 2891 3350 3566 3697 3724 4533
Size: 56 321 1825 459 216 131 27 809
Cuts at: 4533 4666 5125 5334 689
Size: 133 459 209 2305
Fragments arranged by size:
2305 1825 809 459 459 321 216 209
133 131 56 27
TatI w'GTAC_w
Cuts at: 1369 2840 6465 1369
Size: 1471 3625 1854
Fragments arranged by size:
3625 1854 1471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1820 1852 2135 2167 2193 2225 3152 3184 3571
Size: 32 283 32 26 32 927 32 387
Cuts at: 3571 3603 6467 6499 1820
Size: 32 2864 32 2271
Fragments arranged by size:
2864 2271 927 387 283 32 32 32
32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 3118 3816 4747 3118
Size: 698 931 5321
Fragments arranged by size:
5321 931 698
XbaI T'CTAG_A
Cuts at: 3981 3981
Size: 6950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2823 4445 2823
Size: 1622 5328
XmaI C'CCGG_G
Cuts at: 266 788 1145 1758 3970 266
Size: 522 357 613 2212 3246
Fragments arranged by size:
3246 2212 613 522 357
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI BaeI BfrBI BmtI BsiWI
BsrGI BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PshAI PsiI
PspXI PsrI SacII SexAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI