Restriction map for
pF1KB7325
(Circular) (Six-base) MAPSORT of: pF1KB7325.seq Check: 6345 from: 1 to: 8060
pF1KB7325 8060 bp
With 182 enzymes: SgfI *
March 9, 2009 15:47 ..
AccI GT'mk_AC
Cuts at: 995 3776 3873 5098 995
Size: 2781 97 1225 3957
Fragments arranged by size:
3957 2781 1225 97
Acc65I G'GTAC_C
Cuts at: 5076 5076
Size: 8060
AclI AA'CG_TT
Cuts at: 705 7900 705
Size: 7195 865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1784 1793 2348 2774 2871 2924 3283 3664
Size: 748 9 555 426 97 53 359 381
Cuts at: 3664 4649 4663 4765 5885 6317 7064 7332 1036
Size: 985 14 102 1120 432 747 268 1764
Fragments arranged by size:
1764 1120 985 748 747 555 432 426
381 359 268 102 97 53 14 9
AfeI AGC'GCT
Cuts at: 2257 2257
Size: 8060
AflIII A'CryG_T
Cuts at: 3528 5412 6516 7493 3528
Size: 1884 1104 977 4095
Fragments arranged by size:
4095 1884 1104 977
AgeI A'CCGG_T
Cuts at: 249 6448 249
Size: 6199 1861
AleI CACnn'nnGTG
Cuts at: 1253 4456 4912 1253
Size: 3203 456 4401
Fragments arranged by size:
4401 3203 456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1482 1482
Size: 8060
AlwNI CAG_nnn'CTG
Cuts at: 195 588 1018 1335 2371 2559 2582 4575 4926
Size: 393 430 317 1036 188 23 1993 351
Cuts at: 4926 5365 6932 195
Size: 439 1567 1323
Fragments arranged by size:
1993 1567 1323 1036 439 430 393 351
317 188 23
ApaI G_GGCC'C
Cuts at: 129 2129 129
Size: 2000 6060
ApaLI G'TGCA_C
Cuts at: 946 2491 6830 946
Size: 1545 4339 2176
Fragments arranged by size:
4339 2176 1545
ApoI r'AATT_y
Cuts at: 905 1144 3504 3835 3930 4826 4973 5064 7413
Size: 239 2360 331 95 896 147 91 2349
Cuts at: 7413 7669 905
Size: 256 1296
Fragments arranged by size:
2360 2349 1296 896 331 256 239 147
95 91
AseI AT'TA_AT
Cuts at: 20 3937 20
Size: 3917 4143
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8060
AvaI C'yCGr_G
Cuts at: 130 1493 2289 5080 130
Size: 1363 796 2791 3110
Fragments arranged by size:
3110 2791 1363 796
BamHI G'GATC_C
Cuts at: 3464 3749 5085 3464
Size: 285 1336 6439
Fragments arranged by size:
6439 1336 285
BanI G'GyrC_C
Cuts at: 1417 5076 5490 5738 5773 1417
Size: 3659 414 248 35 3704
Fragments arranged by size:
3704 3659 414 248 35
BanII G_rGCy'C
Cuts at: 129 263 570 2129 2296 5074 6104 129
Size: 134 307 1559 167 2778 1030 2085
Fragments arranged by size:
2778 2085 1559 1030 307 167 134
BbeI G_GCGC'C
Cuts at: 5494 5742 5494
Size: 248 7812
BbsI GAAGACnn'nnnn_
Cuts at: 2331 3005 3848 2331
Size: 674 843 6543
Fragments arranged by size:
6543 843 674
BbvCI CC'TCA_GC
Cuts at: 1579 2221 1579
Size: 642 7418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1294 1328 4609 4643 1294
Size: 34 3281 34 4711
Fragments arranged by size:
4711 3281 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5958 6719 8060 5958
Size: 761 1341 5958
Fragments arranged by size:
5958 1341 761
BclI T'GATC_A
Cuts at: 933 4043 5580 933
Size: 3110 1537 3413
Fragments arranged by size:
3413 3110 1537
BglI GCCn_nnn'nGGC
Cuts at: 199 1289 199
Size: 1090 6970
BglII A'GATC_T
Cuts at: 3206 3912 5575 7612 3206
Size: 706 1663 2037 3654
Fragments arranged by size:
3654 2037 1663 706
BlpI GC'TnA_GC
Cuts at: 695 1328 5172 695
Size: 633 3844 3583
Fragments arranged by size:
3844 3583 633
Bme1580I G_kGCm'C
Cuts at: 129 950 2129 2271 2495 5685 5778 6834 129
Size: 821 1179 142 224 3190 93 1056 1355
Fragments arranged by size:
3190 1355 1179 1056 821 224 142 93
BmrI ACTGGGnnnn_n'
Cuts at: 1814 2340 2813 4173 5272 5353 5688 7832 1814
Size: 526 473 1360 1099 81 335 2144 2042
Fragments arranged by size:
2144 2042 1360 1099 526 473 335 81
BmtI G_CTAG'C
Cuts at: 4720 4720
Size: 8060
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6427 6427
Size: 8060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 2280 2604 3598 4172 4561 217
Size: 2063 324 994 574 389 3716
Fragments arranged by size:
3716 2063 994 574 389 324
Bpu10I CC'TnA_GC
Cuts at: 569 1279 1579 2221 569
Size: 710 300 642 6408
Fragments arranged by size:
6408 710 642 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 3079 5236 6115 6607 6905 275
Size: 2804 2157 879 492 298 1430
Fragments arranged by size:
2804 2157 1430 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1266 7764 1266
Size: 6498 1562
BsaAI yAC'GTr
Cuts at: 495 5413 495
Size: 4918 3142
BsaBI GATnn'nnATC
Cuts at: 6 5574 7611 6
Size: 5568 2037 455
Fragments arranged by size:
5568 2037 455
BsaHI Gr'CG_yC
Cuts at: 1554 2107 2336 5491 5739 6441 7937 1554
Size: 553 229 3155 248 702 1496 1677
Fragments arranged by size:
3155 1677 1496 702 553 248 229
BsaWI w'CCGG_w
Cuts at: 153 249 5249 5770 6448 6722 6869 7280 153
Size: 96 5000 521 678 274 147 411 933
Fragments arranged by size:
5000 933 678 521 411 274 147 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1465 1495 4679 4709 4717 4747 1465
Size: 30 3184 30 8 30 4778
Fragments arranged by size:
4778 3184 30 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 589 825 3142 3831 4009 589
Size: 236 2317 689 178 4640
Fragments arranged by size:
4640 2317 689 236 178
BseYI C'CCAG_C
Cuts at: 112 1697 1730 5485 6243 6820 7316 112
Size: 1585 33 3755 758 577 496 856
Fragments arranged by size:
3755 1585 856 758 577 496 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 1256 1339 1948 2020 2135 3136 4090 517
Size: 739 83 609 72 115 1001 954 4487
Fragments arranged by size:
4487 1001 954 739 609 115 83 72
BsiEI CG_ry'CG
Cuts at: 104 141 235 2092 5602 5648 6856 104
Size: 37 94 1857 3510 46 1208 1308
Fragments arranged by size:
3510 1857 1308 1208 94 46 37
BsiHKAI G_wGCw'C
Cuts at: 950 1250 2245 2330 2495 2533 3401 5074 5852
Size: 300 995 85 165 38 868 1673 778
Cuts at: 5852 6042 6834 950
Size: 190 792 2176
Fragments arranged by size:
2176 1673 995 868 792 778 300 190
165 85 38
Bsp1286I G_dGCh'C
Cuts at: 129 263 570 950 1250 2129 2245 2271 2296
Size: 134 307 380 300 879 116 26 25
Cuts at: 2296 2330 2495 2533 3401 5074 5685 5778 5852
Size: 34 165 38 868 1673 611 93 74
Cuts at: 5852 6042 6104 6834 129
Size: 190 62 730 1355
Fragments arranged by size:
1673 1355 879 868 730 611 380 307
300 190 165 134 116 93 74 62
38 34 26 25
BspEI T'CCGG_A
Cuts at: 153 7280 153
Size: 7127 933
BspHI T'CATG_A
Cuts at: 543 4443 7236 543
Size: 3900 2793 1367
Fragments arranged by size:
3900 2793 1367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 1307 3467 4175 5110 5324 5626 6007 236
Size: 1071 2160 708 935 214 302 381 2289
Fragments arranged by size:
2289 2160 1071 935 708 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2079 2088 6352 6406 7890 8055 50
Size: 2029 9 4264 54 1484 165 55
Fragments arranged by size:
4264 2029 1484 165 55 54 9
BsrDI GCAATG_nn'
Cuts at: 1202 2445 5972 1202
Size: 1243 3527 3290
Fragments arranged by size:
3527 3290 1243
BsrFI r'CCGG_y
Cuts at: 249 1811 1988 2091 2163 2284 6058 6239 6448
Size: 1562 177 103 72 121 3774 181 209
Cuts at: 6448 249
Size: 1861
Fragments arranged by size:
3774 1861 1562 209 181 177 121 103
72
BsrGI T'GTAC_A
Cuts at: 5018 5018
Size: 8060
BssSI C'ACGA_G
Cuts at: 181 2083 6331 6689 181
Size: 1902 4248 358 1552
Fragments arranged by size:
4248 1902 1552 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3147 5437 3147
Size: 2290 5770
BstBI TT'CG_AA
Cuts at: 6421 6421
Size: 8060
BstXI CCAn_nnnn'nTGG
Cuts at: 1230 1230
Size: 8060
BstYI r'GATC_y
Cuts at: 1 2880 2948 2973 3206 3464 3749 3912 4839
Size: 2879 68 25 233 258 285 163 927
Cuts at: 4839 5085 5552 5575 5910 6156 7168 7254 7266
Size: 246 467 23 335 246 1012 86 12
Cuts at: 7266 7612 1
Size: 346 449
Fragments arranged by size:
2879 1012 927 467 449 346 335 285
258 246 246 233 163 86 68 25
23 12
Bsu36I CC'TnA_GG
Cuts at: 837 837
Size: 8060
BtgI C'CryG_G
Cuts at: 107 120 2927 4683 107
Size: 13 2807 1756 3484
Fragments arranged by size:
3484 2807 1756 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2315 6010 6191 6354 2315
Size: 3695 181 163 4021
Fragments arranged by size:
4021 3695 181 163
BtsI GCAGTG_nn'
Cuts at: 975 1964 2020 2816 3626 4214 4637 4821 5303
Size: 989 56 796 810 588 423 184 482
Cuts at: 5303 975
Size: 3732
Fragments arranged by size:
3732 989 810 796 588 482 423 184
56
ClaI AT'CG_AT
Cuts at: 3236 3555 3236
Size: 319 7741
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3464 3499 4203 4238 3464
Size: 35 704 35 7286
Fragments arranged by size:
7286 704 35 35
DraI TTT'AAA
Cuts at: 3333 3509 5059 3333
Size: 176 1550 6334
Fragments arranged by size:
6334 1550 176
DrdI GACnn_nn'nnGTC
Cuts at: 2025 5766 6624 2025
Size: 3741 858 3461
Fragments arranged by size:
3741 3461 858
EaeI y'GGCC_r
Cuts at: 138 232 446 1982 2089 2132 2261 4320 5645
Size: 94 214 1536 107 43 129 2059 1325
Cuts at: 5645 5819 6210 6237 7582 138
Size: 174 391 27 1345 616
Fragments arranged by size:
2059 1536 1345 1325 616 391 214 174
129 107 94 43 27
EagI C'GGCC_G
Cuts at: 138 232 2089 5645 138
Size: 94 1857 3556 2553
Fragments arranged by size:
3556 2553 1857 94
EarI CTCTTCn'nnn_
Cuts at: 605 653 665 2521 2890 6083 6293 605
Size: 48 12 1856 369 3193 210 2372
Fragments arranged by size:
3193 2372 1856 369 210 48 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6268 6578 6724 7870 6268
Size: 310 146 1146 6458
Fragments arranged by size:
6458 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5072 5072
Size: 8060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1036 1784 1793 2280 2348 2604 2774 2871
Size: 819 748 9 487 68 256 170 97
Cuts at: 2871 2924 3283 3598 3664 4172 4561 4649 4663
Size: 53 359 315 66 508 389 88 14
Cuts at: 4663 4765 5885 6317 7064 7332 217
Size: 102 1120 432 747 268 945
Fragments arranged by size:
1120 945 819 748 747 508 487 432
389 359 315 268 256 170 102 97
88 68 66 53 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 840 2134 3994 840
Size: 1294 1860 4906
Fragments arranged by size:
4906 1860 1294
EcoO109I rG'GnC_Cy
Cuts at: 125 126 192 753 1047 1291 1380 2126 5199
Size: 1 66 561 294 244 89 746 3073
Cuts at: 5199 125
Size: 2986
Fragments arranged by size:
3073 2986 746 561 294 244 89 66
1
EcoRI G'AATT_C
Cuts at: 1144 5064 1144
Size: 3920 4140
EcoRV GAT'ATC
Cuts at: 4250 4250
Size: 8060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3288 3996 6079 3288
Size: 708 2083 5269
Fragments arranged by size:
5269 2083 708
FspI TGC'GCA
Cuts at: 5841 5841
Size: 8060
HaeII r_GCGC'y
Cuts at: 1455 2259 4755 5494 5742 6764 7743 1455
Size: 804 2496 739 248 1022 979 1772
Fragments arranged by size:
2496 1772 1022 979 804 739 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 918 950 1904 1936 2558 2590 2709
Size: 32 159 32 954 32 622 32 119
Cuts at: 2709 2741 2904 2936 4234 4266 4717 4749 5902
Size: 32 163 32 1298 32 451 32 1153
Cuts at: 5902 5932 5934 5964 727
Size: 30 2 30 2823
Fragments arranged by size:
2823 1298 1153 954 622 451 163 159
119 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2664 3874 5099 2664
Size: 1210 1225 5625
Fragments arranged by size:
5625 1225 1210
HindIII A'AGCT_T
Cuts at: 4613 4613
Size: 8060
KasI G'GCGC_C
Cuts at: 5490 5738 5490
Size: 248 7812
KpnI G_GTAC'C
Cuts at: 5080 5080
Size: 8060
MluI A'CGCG_T
Cuts at: 7493 7493
Size: 8060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 572 1919 3984 6731 6915 572
Size: 1347 2065 2747 184 1717
Fragments arranged by size:
2747 2065 1717 1347 184
MscI TGG'CCA
Cuts at: 448 2134 4322 5821 448
Size: 1686 2188 1499 2687
Fragments arranged by size:
2687 2188 1686 1499
MslI CAynn'nnrTG
Cuts at: 1175 1228 1240 1253 1300 1924 2462 2509 3196
Size: 53 12 13 47 624 538 47 687
Cuts at: 3196 3308 4456 4878 4912 6049 6176 1175
Size: 112 1148 422 34 1137 127 3059
Fragments arranged by size:
3059 1148 1137 687 624 538 422 127
112 53 47 47 34 13 12
MspA1I CmG'CkG
Cuts at: 1636 3431 4150 4923 5171 5485 5845 6858 7103
Size: 1795 719 773 248 314 360 1013 245
Cuts at: 7103 1636
Size: 2593
Fragments arranged by size:
2593 1795 1013 773 719 360 314 248
245
NaeI GCC'GGC
Cuts at: 1990 2093 2165 6241 1990
Size: 103 72 4076 3809
Fragments arranged by size:
4076 3809 103 72
NarI GG'CG_CC
Cuts at: 5491 5739 5491
Size: 248 7812
NcoI C'CATG_G
Cuts at: 107 2927 4683 107
Size: 2820 1756 3484
Fragments arranged by size:
3484 2820 1756
NgoMIV G'CCGG_C
Cuts at: 1988 2091 2163 6239 1988
Size: 103 72 4076 3809
Fragments arranged by size:
4076 3809 103 72
NheI G'CTAG_C
Cuts at: 4716 4716
Size: 8060
NspI r_CATG'y
Cuts at: 3022 3307 5113 5613 6520 3022
Size: 285 1806 500 907 4562
Fragments arranged by size:
4562 1806 907 500 285
PciI A'CATG_T
Cuts at: 6516 6516
Size: 8060
PflMI CCAn_nnn'nTGG
Cuts at: 2877 4101 2877
Size: 1224 6836
PmeI GTTT'AAAC
Cuts at: 5059 5059
Size: 8060
PmlI CAC'GTG
Cuts at: 495 495
Size: 8060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7199 7231 7199
Size: 32 8028
PpuMI rG'GwC_Cy
Cuts at: 753 1047 1380 753
Size: 294 333 7433
Fragments arranged by size:
7433 333 294
PshAI GACnn'nnGTC
Cuts at: 4585 4585
Size: 8060
PsiI TTA'TAA
Cuts at: 1526 1723 4979 1526
Size: 197 3256 4607
Fragments arranged by size:
4607 3256 197
PspOMI G'GGCC_C
Cuts at: 125 2125 125
Size: 2000 6060
PstI C_TGCA'G
Cuts at: 4438 4571 5107 5792 4438
Size: 133 536 685 6706
Fragments arranged by size:
6706 685 536 133
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8060
PvuII CAG'CTG
Cuts at: 1636 4150 4923 5485 5845 1636
Size: 2514 773 562 360 3851
Fragments arranged by size:
3851 2514 773 562 360
RsrII CG'GwC_CG
Cuts at: 247 6255 247
Size: 6008 2052
SacI G_AGCT'C
Cuts at: 5074 5074
Size: 8060
SalI G'TCGA_C
Cuts at: 3872 5097 3872
Size: 1225 6835
SapI GCTCTTCn'nnn_
Cuts at: 605 2521 6083 6293 605
Size: 1916 3562 210 2372
Fragments arranged by size:
3562 2372 1916 210
SbfI CC_TGCA'GG
Cuts at: 5107 5107
Size: 8060
ScaI AGT'ACT
Cuts at: 2518 4432 7577 2518
Size: 1914 3145 3001
Fragments arranged by size:
3145 3001 1914
SfcI C'TryA_G
Cuts at: 33 187 1437 4238 4434 4567 5025 5040 5103
Size: 154 1250 2801 196 133 458 15 63
Cuts at: 5103 5788 6781 6972 33
Size: 685 993 191 1121
Fragments arranged by size:
2801 1250 1121 993 685 458 196 191
154 133 63 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 199 199
Size: 8060
SfoI GGC'GCC
Cuts at: 5492 5740 5492
Size: 248 7812
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8060
SmaI CCC'GGG
Cuts at: 132 2291 5082 132
Size: 2159 2791 3110
Fragments arranged by size:
3110 2791 2159
SmlI C'TyrA_G
Cuts at: 290 3094 5215 6130 6622 6884 290
Size: 2804 2121 915 492 262 1466
Fragments arranged by size:
2804 2121 1466 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8060
SphI G_CATG'C
Cuts at: 3022 5113 5613 3022
Size: 2091 500 5469
Fragments arranged by size:
5469 2091 500
SrfI GCCC'GGGC
Cuts at: 2291 2291
Size: 8060
SspI AAT'ATT
Cuts at: 4859 4859
Size: 8060
StuI AGG'CCT
Cuts at: 296 1343 3184 296
Size: 1047 1841 5172
Fragments arranged by size:
5172 1841 1047
StyI C'CwwG_G
Cuts at: 107 1584 2135 2844 2927 4683 5194 5548 107
Size: 1477 551 709 83 1756 511 354 2619
Fragments arranged by size:
2619 1756 1477 709 551 511 354 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2159 2229 5643 5776 6235 6444 2159
Size: 70 3414 133 459 209 3775
Fragments arranged by size:
3775 3414 459 209 133 70
TatI w'GTAC_w
Cuts at: 2516 3358 4430 4563 5018 7575 2516
Size: 842 1072 133 455 2557 3001
Fragments arranged by size:
3001 2557 1072 842 455 133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4679 4711 7577 7609 4679
Size: 32 2866 32 5130
Fragments arranged by size:
5130 2866 32 32
Tth111I GACn'n_nGTC
Cuts at: 2572 5857 2572
Size: 3285 4775
XbaI T'CTAG_A
Cuts at: 5091 5091
Size: 8060
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3573 5555 3573
Size: 1982 6078
XmaI C'CCGG_G
Cuts at: 130 2289 5080 130
Size: 2159 2791 3110
Fragments arranged by size:
3110 2791 2159
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BfrBI BmgBI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
DraIII FseI FspAI HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PfoI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SwaI XhoI XmnI ZraI