Restriction map for
pF1KB7328
(Circular) (Six-base) MAPSORT of: pF1KB7328.seq Check: 5083 from: 1 to: 7190
pF1KB7328 7190 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 329 349 1958 2378 4228 329
Size: 20 1609 420 1850 3291
Fragments arranged by size:
3291 1850 1609 420 20
Acc65I G'GTAC_C
Cuts at: 2480 4206 2480
Size: 1726 5464
AclI AA'CG_TT
Cuts at: 7030 7030
Size: 7190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 1334 2523 3456 4142 5015 5447 6194 6462
Size: 1120 1189 933 686 873 432 747 268
Cuts at: 6462 214
Size: 942
Fragments arranged by size:
1189 1120 942 933 873 747 686 432
268
AfeI AGC'GCT
Cuts at: 2532 2532
Size: 7190
AflIII A'CryG_T
Cuts at: 471 2456 4542 5646 6623 471
Size: 1985 2086 1104 977 1038
Fragments arranged by size:
2086 1985 1104 1038 977
AgeI A'CCGG_T
Cuts at: 5578 5578
Size: 7190
AhdI GACnn_n'nnGTC
Cuts at: 1995 1995
Size: 7190
AleI CACnn'nnGTG
Cuts at: 292 3364 292
Size: 3072 4118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2827 2859 2827
Size: 32 7158
AlwNI CAG_nnn'CTG
Cuts at: 1402 2546 3099 3795 4147 4495 6062 1402
Size: 1144 553 696 352 348 1567 2530
Fragments arranged by size:
2530 1567 1144 696 553 352 348
ApaI G_GGCC'C
Cuts at: 651 890 2597 651
Size: 239 1707 5244
Fragments arranged by size:
5244 1707 239
ApaLI G'TGCA_C
Cuts at: 5960 5960
Size: 7190
ApoI r'AATT_y
Cuts at: 457 1346 2371 3355 4194 6543 6799 457
Size: 889 1025 984 839 2349 256 848
Fragments arranged by size:
2349 1025 984 889 848 839 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7190
AvaI C'yCGr_G
Cuts at: 3911 4210 3911
Size: 299 6891
AvrII C'CTAG_G
Cuts at: 1686 1686
Size: 7190
BamHI G'GATC_C
Cuts at: 2232 3714 3753 4215 2232
Size: 1482 39 462 5207
Fragments arranged by size:
5207 1482 462 39
BanI G'GyrC_C
Cuts at: 2480 4206 4620 4868 4903 2480
Size: 1726 414 248 35 4767
Fragments arranged by size:
4767 1726 414 248 35
BanII G_rGCy'C
Cuts at: 651 890 1669 2284 2578 2597 2794 3035 3918
Size: 239 779 615 294 19 197 241 883
Cuts at: 3918 4204 5234 651
Size: 286 1030 2607
Fragments arranged by size:
2607 1030 883 779 615 294 286 241
239 197 19
BbeI G_GCGC'C
Cuts at: 4624 4872 4624
Size: 248 6942
BbsI GAAGACnn'nnnn_
Cuts at: 202 630 1397 2056 2715 3686 202
Size: 428 767 659 659 971 3706
Fragments arranged by size:
3706 971 767 659 659 428
BciVI GTATCCnnnnn_n'
Cuts at: 5088 5849 7190 5088
Size: 761 1341 5088
Fragments arranged by size:
5088 1341 761
BclI T'GATC_A
Cuts at: 1040 1809 4710 1040
Size: 769 2901 3520
Fragments arranged by size:
3520 2901 769
BglII A'GATC_T
Cuts at: 138 1628 2780 4705 6742 138
Size: 1490 1152 1925 2037 586
Fragments arranged by size:
2037 1925 1490 1152 586
BlpI GC'TnA_GC
Cuts at: 3304 4302 3304
Size: 998 6192
Bme1580I G_kGCm'C
Cuts at: 651 890 2597 4815 4908 5964 651
Size: 239 1707 2218 93 1056 1877
Fragments arranged by size:
2218 1877 1707 1056 239 93
BmgBI CAC'GTC
Cuts at: 2028 4077 2028
Size: 2049 5141
BmrI ACTGGGnnnn_n'
Cuts at: 202 4402 4483 4818 6962 202
Size: 4200 81 335 2144 430
Fragments arranged by size:
4200 2144 430 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 450 588 2500 3937 5557 450
Size: 138 1912 1437 1620 2083
Fragments arranged by size:
2083 1912 1620 1437 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 1834 2309 2495 3817 538
Size: 1296 475 186 1322 3911
Fragments arranged by size:
3911 1322 1296 475 186
Bpu10I CC'TnA_GC
Cuts at: 1861 2056 2393 3376 1861
Size: 195 337 983 5675
Fragments arranged by size:
5675 983 337 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3019 4366 5245 5737 6035 3019
Size: 1347 879 492 298 4174
Fragments arranged by size:
4174 1347 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1874 2563 6894 1874
Size: 689 4331 2170
Fragments arranged by size:
4331 2170 689
BsaAI yAC'GTr
Cuts at: 447 2016 2459 4543 447
Size: 1569 443 2084 3094
Fragments arranged by size:
3094 2084 1569 443
BsaBI GATnn'nnATC
Cuts at: 6 484 4704 6741 6
Size: 478 4220 2037 455
Fragments arranged by size:
4220 2037 478 455
BsaHI Gr'CG_yC
Cuts at: 2009 2705 4621 4869 5571 7067 2009
Size: 696 1916 248 702 1496 2132
Fragments arranged by size:
2132 1916 1496 702 696 248
BsaWI w'CCGG_w
Cuts at: 683 3580 4379 4900 5578 5852 5999 6410 683
Size: 2897 799 521 678 274 147 411 1463
Fragments arranged by size:
2897 1463 799 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3067 3097 3067
Size: 30 7160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1545 1579 2050 2191 2742 3035 3075 3142 3243
Size: 34 471 141 551 293 40 67 101
Cuts at: 3243 1545
Size: 5492
Fragments arranged by size:
5492 551 471 293 141 101 67 40
34
BseYI C'CCAG_C
Cuts at: 187 2553 3451 4615 5373 5950 6446 187
Size: 2366 898 1164 758 577 496 931
Fragments arranged by size:
2366 1164 931 898 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 4008 1579
Size: 2429 4761
BsiEI CG_ry'CG
Cuts at: 104 1968 2003 4732 4778 5986 104
Size: 1864 35 2729 46 1208 1308
Fragments arranged by size:
2729 1864 1308 1208 46 35
BsiHKAI G_wGCw'C
Cuts at: 770 1669 2399 3035 3171 3918 4204 4982 5172
Size: 899 730 636 136 747 286 778 190
Cuts at: 5172 5964 770
Size: 792 1996
Fragments arranged by size:
1996 899 792 778 747 730 636 286
190 136
BsiWI C'GTAC_G
Cuts at: 866 866
Size: 7190
BsmI GAATG_Cn'
Cuts at: 3412 3665 3412
Size: 253 6937
BsmBI CGTCTCn'nnnn_
Cuts at: 1206 3337 1206
Size: 2131 5059
Bsp1286I G_dGCh'C
Cuts at: 651 770 890 1669 2284 2399 2578 2597 2794
Size: 119 120 779 615 115 179 19 197
Cuts at: 2794 3035 3171 3918 4204 4815 4908 4982 5172
Size: 241 136 747 286 611 93 74 190
Cuts at: 5172 5234 5964 651
Size: 62 730 1877
Fragments arranged by size:
1877 779 747 730 615 611 286 241
197 190 179 136 120 119 115 93
74 62 19
BspEI T'CCGG_A
Cuts at: 3580 6410 3580
Size: 2830 4360
BspHI T'CATG_A
Cuts at: 3203 3809 6366 3203
Size: 606 2557 4027
Fragments arranged by size:
4027 2557 606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1036 1988 2219 3780 4240 4454 4756 5137 1036
Size: 952 231 1561 460 214 302 381 3089
Fragments arranged by size:
3089 1561 952 460 381 302 231 214
BsrBI CCG'CTC
Cuts at: 50 1902 3308 5482 5536 7020 7185 50
Size: 1852 1406 2174 54 1484 165 55
Fragments arranged by size:
2174 1852 1484 1406 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1277 1313 2264 5102 1277
Size: 36 951 2838 3365
Fragments arranged by size:
3365 2838 951 36
BsrFI r'CCGG_y
Cuts at: 155 291 311 528 3056 5188 5369 5578 155
Size: 136 20 217 2528 2132 181 209 1767
Fragments arranged by size:
2528 2132 1767 217 209 181 136 20
BsrGI T'GTAC_A
Cuts at: 2669 2669
Size: 7190
BssSI C'ACGA_G
Cuts at: 2929 5461 5819 2929
Size: 2532 358 4300
Fragments arranged by size:
4300 2532 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4567 4567
Size: 7190
BstBI TT'CG_AA
Cuts at: 5551 5551
Size: 7190
BstEII G'GTnAC_C
Cuts at: 2079 3059 3237 2079
Size: 980 178 6032
Fragments arranged by size:
6032 980 178
BstXI CCAn_nnnn'nTGG
Cuts at: 243 2742 2853 243
Size: 2499 111 4580
Fragments arranged by size:
4580 2499 111
BstYI r'GATC_y
Cuts at: 1 138 163 1628 1760 2232 2780 3714 3753
Size: 137 25 1465 132 472 548 934 39
Cuts at: 3753 4215 4682 4705 5040 5286 6298 6384 6396
Size: 462 467 23 335 246 1012 86 12
Cuts at: 6396 6742 1
Size: 346 449
Fragments arranged by size:
1465 1012 934 548 472 467 462 449
346 335 246 137 132 86 39 25
23 12
BtgI C'CryG_G
Cuts at: 107 1232 2003 3365 3456 107
Size: 1125 771 1362 91 3841
Fragments arranged by size:
3841 1362 1125 771 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4023 4171 5140 5321 5484 4023
Size: 148 969 181 163 5729
Fragments arranged by size:
5729 969 181 163 148
BtsI GCAGTG_nn'
Cuts at: 2672 4433 2672
Size: 1761 5429
DraI TTT'AAA
Cuts at: 4189 4189
Size: 7190
DraIII CAC_nnn'GTG
Cuts at: 450 450
Size: 7190
DrdI GACnn_nn'nnGTC
Cuts at: 4896 5754 4896
Size: 858 6332
EaeI y'GGCC_r
Cuts at: 491 677 947 2000 2972 4775 4949 5340 5367
Size: 186 270 1053 972 1803 174 391 27
Cuts at: 5367 6712 491
Size: 1345 969
Fragments arranged by size:
1803 1345 1053 972 969 391 270 186
174 27
EagI C'GGCC_G
Cuts at: 2000 4775 2000
Size: 2775 4415
EarI CTCTTCn'nnn_
Cuts at: 398 2500 2515 3923 5213 5423 398
Size: 2102 15 1408 1290 210 2165
Fragments arranged by size:
2165 2102 1408 1290 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 174 5398 5708 5854 7000 174
Size: 5224 310 146 1146 364
Fragments arranged by size:
5224 1146 364 310 146
EcoICRI GAG'CTC
Cuts at: 1667 3033 3916 4202 1667
Size: 1366 883 286 4655
Fragments arranged by size:
4655 1366 883 286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 538 1334 1834 2309 2495 2523 3456 3817
Size: 324 796 500 475 186 28 933 361
Cuts at: 3817 4142 5015 5447 6194 6462 214
Size: 325 873 432 747 268 942
Fragments arranged by size:
942 933 873 796 747 500 475 432
361 325 324 268 186 28
EcoNI CCTnn'n_nnAGG
Cuts at: 222 1032 1852 222
Size: 810 820 5560
Fragments arranged by size:
5560 820 810
EcoO109I rG'GnC_Cy
Cuts at: 121 421 569 648 886 1857 1905 2594 3096
Size: 300 148 79 238 971 48 689 502
Cuts at: 3096 3431 4329 121
Size: 335 898 2982
Fragments arranged by size:
2982 971 898 689 502 335 300 238
148 79 48
EcoRI G'AATT_C
Cuts at: 4194 4194
Size: 7190
EcoRV GAT'ATC
Cuts at: 908 3660 908
Size: 2752 4438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2046 5209 2046
Size: 3163 4027
FspI TGC'GCA
Cuts at: 4971 4971
Size: 7190
HaeII r_GCGC'y
Cuts at: 700 2190 2534 4624 4872 5894 6873 700
Size: 1490 344 2090 248 1022 979 1017
Fragments arranged by size:
2090 1490 1022 1017 979 344 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 374 406 1205 1237 1981 2013 2560 2592 5032
Size: 32 799 32 744 32 547 32 2440
Cuts at: 5032 5062 5064 5094 374
Size: 30 2 30 2470
Fragments arranged by size:
2470 2440 799 744 547 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 350 4229 350
Size: 3879 3311
HindIII A'AGCT_T
Cuts at: 1605 1605
Size: 7190
KasI G'GCGC_C
Cuts at: 4620 4868 4620
Size: 248 6942
KpnI G_GTAC'C
Cuts at: 2484 4210 2484
Size: 1726 5464
MluI A'CGCG_T
Cuts at: 471 6623 471
Size: 6152 1038
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1988 2546 3546 5861 6045 1988
Size: 558 1000 2315 184 3133
Fragments arranged by size:
3133 2315 1000 558 184
MscI TGG'CCA
Cuts at: 493 949 2974 4951 493
Size: 456 2025 1977 2732
Fragments arranged by size:
2732 2025 1977 456
MslI CAynn'nnrTG
Cuts at: 292 511 1772 2695 2740 3116 3364 3773 3823
Size: 219 1261 923 45 376 248 409 50
Cuts at: 3823 5179 5306 292
Size: 1356 127 2176
Fragments arranged by size:
2176 1356 1261 923 409 376 248 219
127 50 45
MspA1I CmG'CkG
Cuts at: 1701 3303 3897 4301 4615 4975 5988 6233 1701
Size: 1602 594 404 314 360 1013 245 2658
Fragments arranged by size:
2658 1602 1013 594 404 360 314 245
NaeI GCC'GGC
Cuts at: 157 5371 157
Size: 5214 1976
NarI GG'CG_CC
Cuts at: 4621 4869 4621
Size: 248 6942
NcoI C'CATG_G
Cuts at: 107 107
Size: 7190
NdeI CA'TA_TG
Cuts at: 354 354
Size: 7190
NgoMIV G'CCGG_C
Cuts at: 155 5369 155
Size: 5214 1976
NspI r_CATG'y
Cuts at: 2656 4243 4743 5650 2656
Size: 1587 500 907 4196
Fragments arranged by size:
4196 1587 907 500
PasI CC'CwG_GG
Cuts at: 2598 2598
Size: 7190
PciI A'CATG_T
Cuts at: 5646 5646
Size: 7190
PflMI CCAn_nnn'nTGG
Cuts at: 3446 3631 3446
Size: 185 7005
PfoI T'CCnGG_A
Cuts at: 2325 3026 2325
Size: 701 6489
PmeI GTTT'AAAC
Cuts at: 4189 4189
Size: 7190
PmlI CAC'GTG
Cuts at: 447 2016 447
Size: 1569 5621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6329 6361 6329
Size: 32 7158
PpuMI rG'GwC_Cy
Cuts at: 121 421 569 1857 3431 121
Size: 300 148 1288 1574 3880
Fragments arranged by size:
3880 1574 1288 300 148
PshAI GACnn'nnGTC
Cuts at: 2071 2645 2071
Size: 574 6616
PspOMI G'GGCC_C
Cuts at: 647 886 2593 647
Size: 239 1707 5244
Fragments arranged by size:
5244 1707 239
PstI C_TGCA'G
Cuts at: 1706 2903 4237 4922 1706
Size: 1197 1334 685 3974
Fragments arranged by size:
3974 1334 1197 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7190
PvuII CAG'CTG
Cuts at: 1701 3303 3897 4615 4975 1701
Size: 1602 594 718 360 3916
Fragments arranged by size:
3916 1602 718 594 360
RsrII CG'GwC_CG
Cuts at: 5385 5385
Size: 7190
SacI G_AGCT'C
Cuts at: 1669 3035 3918 4204 1669
Size: 1366 883 286 4655
Fragments arranged by size:
4655 1366 883 286
SalI G'TCGA_C
Cuts at: 348 4227 348
Size: 3879 3311
SapI GCTCTTCn'nnn_
Cuts at: 3923 5213 5423 3923
Size: 1290 210 5690
Fragments arranged by size:
5690 1290 210
SbfI CC_TGCA'GG
Cuts at: 4237 4237
Size: 7190
ScaI AGT'ACT
Cuts at: 3264 6707 3264
Size: 3443 3747
SfcI C'TryA_G
Cuts at: 33 1702 2092 2899 2984 4233 4918 5911 6102
Size: 1669 390 807 85 1249 685 993 191
Cuts at: 6102 33
Size: 1121
Fragments arranged by size:
1669 1249 1121 993 807 685 390 191
85
SfoI GGC'GCC
Cuts at: 4622 4870 4622
Size: 248 6942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7190
SgrAI Cr'CCGG_yG
Cuts at: 291 291
Size: 7190
SmaI CCC'GGG
Cuts at: 4212 4212
Size: 7190
SmlI C'TyrA_G
Cuts at: 3034 4345 5260 5752 6014 3034
Size: 1311 915 492 262 4210
Fragments arranged by size:
4210 1311 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7190
SphI G_CATG'C
Cuts at: 4243 4743 4243
Size: 500 6690
StuI AGG'CCT
Cuts at: 433 3278 433
Size: 2845 4345
StyI C'CwwG_G
Cuts at: 107 707 1544 1686 2846 3440 4324 4678 107
Size: 600 837 142 1160 594 884 354 2619
Fragments arranged by size:
2619 1160 884 837 600 594 354 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 103 249 1396 2635 2655 3088 4773 4906 5365
Size: 146 1147 1239 20 433 1685 133 459
Cuts at: 5365 5574 103
Size: 209 1719
Fragments arranged by size:
1719 1685 1239 1147 459 433 209 146
133 20
TatI w'GTAC_w
Cuts at: 2669 3262 6705 2669
Size: 593 3443 3154
Fragments arranged by size:
3443 3154 593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2373 2405 2574 2601 2606 2633 3067 3099 6707
Size: 32 169 27 5 27 434 32 3608
Cuts at: 6707 6739 2373
Size: 32 2824
Fragments arranged by size:
3608 2824 434 169 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 4987 4987
Size: 7190
XbaI T'CTAG_A
Cuts at: 4221 4221
Size: 7190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 754 1305 3096 3963 4685 754
Size: 551 1791 867 722 3259
Fragments arranged by size:
3259 1791 867 722 551
XmaI C'CCGG_G
Cuts at: 4210 4210
Size: 7190
XmnI GAAnn'nnTTC
Cuts at: 3576 3576
Size: 7190
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AlfI AscI BaeI BbvCI BcgI
BfrBI BglI BmtI BssHII BstZ17I Bsu36I ClaI CspCI
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI SwaI XhoI ZraI