Restriction map for
pF1KB7329
(Circular) (Six-base) MAPSORT of: pF1KB7329.seq Check: 7171 from: 1 to: 5813
pF1KB7329 5813 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1601 1601
Size: 5813
AatII G_ACGT'C
Cuts at: 1468 1468
Size: 5813
AccI GT'mk_AC
Cuts at: 787 1645 2851 787
Size: 858 1206 3749
Fragments arranged by size:
3749 1206 858
Acc65I G'GTAC_C
Cuts at: 2625 2829 2625
Size: 204 5609
AclI AA'CG_TT
Cuts at: 5653 5653
Size: 5813
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 470 1330 1372 2053 2605 3638 4070 4817
Size: 104 860 42 681 552 1033 432 747
Cuts at: 4817 5085 366
Size: 268 1094
Fragments arranged by size:
1094 1033 860 747 681 552 432 268
104 42
AflIII A'CryG_T
Cuts at: 3165 4269 5246 3165
Size: 1104 977 3732
Fragments arranged by size:
3732 1104 977
AgeI A'CCGG_T
Cuts at: 4201 4201
Size: 5813
AleI CACnn'nnGTG
Cuts at: 2464 2464
Size: 5813
AlwNI CAG_nnn'CTG
Cuts at: 2470 3118 4685 2470
Size: 648 1567 3598
Fragments arranged by size:
3598 1567 648
ApaI G_GGCC'C
Cuts at: 2698 2698
Size: 5813
ApaLI G'TGCA_C
Cuts at: 4583 4583
Size: 5813
ApoI r'AATT_y
Cuts at: 663 1266 1887 2817 5166 5422 663
Size: 603 621 930 2349 256 1054
Fragments arranged by size:
2349 1054 930 621 603 256
AseI AT'TA_AT
Cuts at: 20 563 20
Size: 543 5270
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5813
AvaI C'yCGr_G
Cuts at: 1926 2273 2833 1926
Size: 347 560 4906
Fragments arranged by size:
4906 560 347
BamHI G'GATC_C
Cuts at: 2838 2838
Size: 5813
BanI G'GyrC_C
Cuts at: 2625 2673 2829 3243 3491 3526 2625
Size: 48 156 414 248 35 4912
Fragments arranged by size:
4912 414 248 156 48 35
BanII G_rGCy'C
Cuts at: 1485 1777 2698 2827 3857 1485
Size: 292 921 129 1030 3441
Fragments arranged by size:
3441 1030 921 292 129
BbeI G_GCGC'C
Cuts at: 3247 3495 3247
Size: 248 5565
BbsI GAAGACnn'nnnn_
Cuts at: 296 304 1318 1468 1824 1910 2448 296
Size: 8 1014 150 356 86 538 3661
Fragments arranged by size:
3661 1014 538 356 150 86 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1360 1394 1360
Size: 34 5779
BciVI GTATCCnnnnn_n'
Cuts at: 3711 4472 5813 3711
Size: 761 1341 3711
Fragments arranged by size:
3711 1341 761
BclI T'GATC_A
Cuts at: 3333 3333
Size: 5813
BfrBI ATG'CAT
Cuts at: 192 947 192
Size: 755 5058
BglII A'GATC_T
Cuts at: 3328 5365 3328
Size: 2037 3776
BlpI GC'TnA_GC
Cuts at: 2925 2925
Size: 5813
Bme1580I G_kGCm'C
Cuts at: 2102 2379 2698 3438 3531 4587 2102
Size: 277 319 740 93 1056 3328
Fragments arranged by size:
3328 1056 740 319 277 93
BmrI ACTGGGnnnn_n'
Cuts at: 2217 2283 3025 3106 3441 5585 2217
Size: 66 742 81 335 2144 2445
Fragments arranged by size:
2445 2144 742 335 81 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1585 4180 1585
Size: 2595 3218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 940 2036 144
Size: 796 1096 3921
Fragments arranged by size:
3921 1096 796
Bpu10I CC'TnA_GC
Cuts at: 1070 1070
Size: 5813
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1257 1474 2617 2989 3868 4360 4658 1257
Size: 217 1143 372 879 492 298 2412
Fragments arranged by size:
2412 1143 879 492 372 298 217
BsaI GGTCTCn'nnnn_
Cuts at: 1670 2328 5517 1670
Size: 658 3189 1966
Fragments arranged by size:
3189 1966 658
BsaAI yAC'GTr
Cuts at: 1300 3166 1300
Size: 1866 3947
BsaBI GATnn'nnATC
Cuts at: 6 3327 5364 6
Size: 3321 2037 455
Fragments arranged by size:
3321 2037 455
BsaHI Gr'CG_yC
Cuts at: 647 1465 3244 3492 4194 5690 647
Size: 818 1779 248 702 1496 770
Fragments arranged by size:
1779 1496 818 770 702 248
BsaWI w'CCGG_w
Cuts at: 2022 3002 3523 4201 4475 4622 5033 2022
Size: 980 521 678 274 147 411 2802
Fragments arranged by size:
2802 980 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1450 1480 2709 2739 1450
Size: 30 1229 30 4524
Fragments arranged by size:
4524 1229 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 362 1465 362
Size: 1103 4710
BseYI C'CCAG_C
Cuts at: 803 2728 2768 3238 3996 4573 5069 803
Size: 1925 40 470 758 577 496 1547
Fragments arranged by size:
1925 1547 758 577 496 470 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1573 2579 2620 1573
Size: 1006 41 4766
Fragments arranged by size:
4766 1006 41
BsiEI CG_ry'CG
Cuts at: 104 2655 3355 3401 4609 104
Size: 2551 700 46 1208 1308
Fragments arranged by size:
2551 1308 1208 700 46
BsiHKAI G_wGCw'C
Cuts at: 968 2827 3605 3795 4587 968
Size: 1859 778 190 792 2194
Fragments arranged by size:
2194 1859 792 778 190
BsmI GAATG_Cn'
Cuts at: 192 1997 192
Size: 1805 4008
BsmBI CGTCTCn'nnnn_
Cuts at: 1492 1492
Size: 5813
Bsp1286I G_dGCh'C
Cuts at: 968 1485 1777 2102 2379 2698 2827 3438 3531
Size: 517 292 325 277 319 129 611 93
Cuts at: 3531 3605 3795 3857 4587 968
Size: 74 190 62 730 2194
Fragments arranged by size:
2194 730 611 517 325 319 292 277
190 129 93 74 62
BspEI T'CCGG_A
Cuts at: 2022 5033 2022
Size: 3011 2802
BspHI T'CATG_A
Cuts at: 2358 4989 2358
Size: 2631 3182
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1601 1707 2863 3077 3379 3760 1601
Size: 106 1156 214 302 381 3654
Fragments arranged by size:
3654 1156 381 302 214 106
BsrBI CCG'CTC
Cuts at: 50 156 2181 2657 4105 4159 5643 5808 50
Size: 106 2025 476 1448 54 1484 165 55
Fragments arranged by size:
2025 1484 1448 476 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 1113 3725 1113
Size: 2612 3201
BsrFI r'CCGG_y
Cuts at: 2650 3811 3992 4201 2650
Size: 1161 181 209 4262
Fragments arranged by size:
4262 1161 209 181
BssSI C'ACGA_G
Cuts at: 4084 4442 4084
Size: 358 5455
BstAPI GCAn_nnn'nTGC
Cuts at: 2284 2470 3190 2284
Size: 186 720 4907
Fragments arranged by size:
4907 720 186
BstBI TT'CG_AA
Cuts at: 4174 4174
Size: 5813
BstXI CCAn_nnnn'nTGG
Cuts at: 2145 2499 2145
Size: 354 5459
BstYI r'GATC_y
Cuts at: 1 321 1149 1176 1565 2019 2507 2538 2546
Size: 320 828 27 389 454 488 31 8
Cuts at: 2546 2838 3305 3328 3663 3909 4921 5007 5019
Size: 292 467 23 335 246 1012 86 12
Cuts at: 5019 5365 1
Size: 346 449
Fragments arranged by size:
1012 828 488 467 454 449 389 346
335 320 292 246 86 31 27 23
12 8
BstZ17I GTA'TAC
Cuts at: 788 788
Size: 5813
BtgI C'CryG_G
Cuts at: 107 882 2102 2579 107
Size: 775 1220 477 3341
Fragments arranged by size:
3341 1220 775 477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3763 3944 4107 3763
Size: 181 163 5469
Fragments arranged by size:
5469 181 163
BtsI GCAGTG_nn'
Cuts at: 1421 1664 2402 2459 2471 2677 3056 1421
Size: 243 738 57 12 206 379 4178
Fragments arranged by size:
4178 738 379 243 206 57 12
DraI TTT'AAA
Cuts at: 1893 2812 1893
Size: 919 4894
DraIII CAC_nnn'GTG
Cuts at: 2673 2673
Size: 5813
DrdI GACnn_nn'nnGTC
Cuts at: 2583 3519 4377 2583
Size: 936 858 4019
Fragments arranged by size:
4019 936 858
EaeI y'GGCC_r
Cuts at: 879 2441 2489 2652 3398 3572 3963 3990 5335
Size: 1562 48 163 746 174 391 27 1345
Cuts at: 5335 879
Size: 1357
Fragments arranged by size:
1562 1357 1345 746 391 174 163 48
27
EagI C'GGCC_G
Cuts at: 2652 3398 2652
Size: 746 5067
EarI CTCTTCn'nnn_
Cuts at: 421 3836 4046 421
Size: 3415 210 2188
Fragments arranged by size:
3415 2188 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2690 4021 4331 4477 5623 2690
Size: 1331 310 146 1146 2880
Fragments arranged by size:
2880 1331 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2825 2825
Size: 5813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 366 470 940 1330 1372 2036 2053 2605
Size: 222 104 470 390 42 664 17 552
Cuts at: 2605 3638 4070 4817 5085 144
Size: 1033 432 747 268 872
Fragments arranged by size:
1033 872 747 664 552 470 432 390
268 222 104 42 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2702 2702
Size: 5813
EcoO109I rG'GnC_Cy
Cuts at: 2694 2695 2753 2952 2694
Size: 1 58 199 5555
Fragments arranged by size:
5555 199 58 1
EcoRI G'AATT_C
Cuts at: 2817 2817
Size: 5813
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1755 3832 1755
Size: 2077 3736
FspI TGC'GCA
Cuts at: 1996 3594 1996
Size: 1598 4215
HaeII r_GCGC'y
Cuts at: 3247 3495 4517 5496 3247
Size: 248 1022 979 3564
Fragments arranged by size:
3564 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 391 423 1327 1359 1450 1482 2340
Size: 32 46 32 904 32 91 32 858
Cuts at: 2340 2372 3655 3685 3687 3717 313
Size: 32 1283 30 2 30 2409
Fragments arranged by size:
2409 1283 904 858 91 46 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1736 2852 1736
Size: 1116 4697
KasI G'GCGC_C
Cuts at: 3243 3491 3243
Size: 248 5565
KpnI G_GTAC'C
Cuts at: 2629 2833 2629
Size: 204 5609
MluI A'CGCG_T
Cuts at: 5246 5246
Size: 5813
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1099 4484 4668 1099
Size: 3385 184 2244
Fragments arranged by size:
3385 2244 184
MscI TGG'CCA
Cuts at: 881 2443 3574 881
Size: 1562 1131 3120
Fragments arranged by size:
3120 1562 1131
MslI CAynn'nnrTG
Cuts at: 197 698 716 1438 2357 2464 3802 3929 197
Size: 501 18 722 919 107 1338 127 2081
Fragments arranged by size:
2081 1338 919 722 501 127 107 18
MspA1I CmG'CkG
Cuts at: 405 738 2422 2924 3238 3598 4611 4856 405
Size: 333 1684 502 314 360 1013 245 1362
Fragments arranged by size:
1684 1362 1013 502 360 333 314 245
NaeI GCC'GGC
Cuts at: 2652 3994 2652
Size: 1342 4471
NarI GG'CG_CC
Cuts at: 3244 3492 3244
Size: 248 5565
NcoI C'CATG_G
Cuts at: 107 882 2579 107
Size: 775 1697 3341
Fragments arranged by size:
3341 1697 775
NdeI CA'TA_TG
Cuts at: 1260 1260
Size: 5813
NgoMIV G'CCGG_C
Cuts at: 2650 3992 2650
Size: 1342 4471
NsiI A_TGCA'T
Cuts at: 194 949 194
Size: 755 5058
NspI r_CATG'y
Cuts at: 1129 2866 3366 4273 1129
Size: 1737 500 907 2669
Fragments arranged by size:
2669 1737 907 500
PasI CC'CwG_GG
Cuts at: 2749 2749
Size: 5813
PciI A'CATG_T
Cuts at: 4269 4269
Size: 5813
PflMI CCAn_nnn'nTGG
Cuts at: 2229 2229
Size: 5813
PfoI T'CCnGG_A
Cuts at: 2042 2549 2042
Size: 507 5306
PmeI GTTT'AAAC
Cuts at: 2812 2812
Size: 5813
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4952 4984 4952
Size: 32 5781
PpuMI rG'GwC_Cy
Cuts at: 2753 2753
Size: 5813
PsiI TTA'TAA
Cuts at: 1540 1540
Size: 5813
PspOMI G'GGCC_C
Cuts at: 2694 2694
Size: 5813
PstI C_TGCA'G
Cuts at: 1097 2466 2618 2684 2860 3545 1097
Size: 1369 152 66 176 685 3365
Fragments arranged by size:
3365 1369 685 176 152 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5813
PvuII CAG'CTG
Cuts at: 405 738 3238 3598 405
Size: 333 2500 360 2620
Fragments arranged by size:
2620 2500 360 333
RsrII CG'GwC_CG
Cuts at: 4008 4008
Size: 5813
SacI G_AGCT'C
Cuts at: 2827 2827
Size: 5813
SalI G'TCGA_C
Cuts at: 2850 2850
Size: 5813
SanDI GG'GwC_CC
Cuts at: 2753 2753
Size: 5813
SapI GCTCTTCn'nnn_
Cuts at: 421 3836 4046 421
Size: 3415 210 2188
Fragments arranged by size:
3415 2188 210
SbfI CC_TGCA'GG
Cuts at: 2860 2860
Size: 5813
ScaI AGT'ACT
Cuts at: 5330 5330
Size: 5813
SexAI A'CCwGG_T
Cuts at: 2669 2669
Size: 5813
SfcI C'TryA_G
Cuts at: 33 493 1093 1320 2462 2614 2680 2856 3541
Size: 460 600 227 1142 152 66 176 685
Cuts at: 3541 4534 4725 33
Size: 993 191 1121
Fragments arranged by size:
1142 1121 993 685 600 460 227 191
176 152 66
SfoI GGC'GCC
Cuts at: 3245 3493 3245
Size: 248 5565
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5813
SmaI CCC'GGG
Cuts at: 2835 2835
Size: 5813
SmlI C'TyrA_G
Cuts at: 1272 1489 2632 2968 3883 4375 4637 1272
Size: 217 1143 336 915 492 262 2448
Fragments arranged by size:
2448 1143 915 492 336 262 217
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5813
SphI G_CATG'C
Cuts at: 2866 3366 2866
Size: 500 5313
SspI AAT'ATT
Cuts at: 1281 1281
Size: 5813
StuI AGG'CCT
Cuts at: 2689 2689
Size: 5813
StyI C'CwwG_G
Cuts at: 107 882 1845 2196 2344 2579 2588 2947 3301
Size: 775 963 351 148 235 9 359 354
Cuts at: 3301 107
Size: 2619
Fragments arranged by size:
2619 963 775 359 354 351 235 148
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 817 1877 2782 3396 3529 3988 4197 817
Size: 1060 905 614 133 459 209 2433
Fragments arranged by size:
2433 1060 905 614 459 209 133
TatI w'GTAC_w
Cuts at: 232 279 2249 5328 232
Size: 47 1970 3079 717
Fragments arranged by size:
3079 1970 717 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 836 868 2657 2689 2709 2741 5330 5362 836
Size: 32 1789 32 20 32 2589 32 1287
Fragments arranged by size:
2589 1789 1287 32 32 32 32 20
Tth111I GACn'n_nGTC
Cuts at: 3610 3610
Size: 5813
XbaI T'CTAG_A
Cuts at: 2844 2844
Size: 5813
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 874 1701 2586 2736 3308 874
Size: 827 885 150 572 3379
Fragments arranged by size:
3379 885 827 572 150
XmaI C'CCGG_G
Cuts at: 2833 2833
Size: 5813
XmnI GAAnn'nnTTC
Cuts at: 1532 1821 2038 1532
Size: 289 217 5307
Fragments arranged by size:
5307 289 217
ZraI GAC'GTC
Cuts at: 1466 1466
Size: 5813
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BglI BmgBI BmtI BsiWI BsrGI BssHII BstEII
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PmlI PshAI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SwaI XhoI