Restriction map for
pF1KB7330
(Circular) (Six-base) MAPSORT of: pF1KB7330.seq Check: 4305 from: 1 to: 5729
pF1KB7330 5729 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AatII G_ACGT'C
Cuts at: 1808 1808
Size: 5729
AccI GT'mk_AC
Cuts at: 137 2147 2767 137
Size: 2010 620 3099
Fragments arranged by size:
3099 2010 620
Acc65I G'GTAC_C
Cuts at: 566 2745 566
Size: 2179 3550
AclI AA'CG_TT
Cuts at: 5569 5569
Size: 5729
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 1081 2496 2505 3554 3986 4733 5001 187
Size: 894 1415 9 1049 432 747 268 915
Fragments arranged by size:
1415 1049 915 894 747 432 268 9
AflIII A'CryG_T
Cuts at: 401 2462 3081 4185 5162 401
Size: 2061 619 1104 977 968
Fragments arranged by size:
2061 1104 977 968 619
AgeI A'CCGG_T
Cuts at: 4117 4117
Size: 5729
AhdI GACnn_n'nnGTC
Cuts at: 1842 1842
Size: 5729
AleI CACnn'nnGTG
Cuts at: 912 912
Size: 5729
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 573 1189 573
Size: 616 5113
AlwNI CAG_nnn'CTG
Cuts at: 723 3034 4601 723
Size: 2311 1567 1851
Fragments arranged by size:
2311 1851 1567
ApaI G_GGCC'C
Cuts at: 483 1379 1897 1930 2660 2717 483
Size: 896 518 33 730 57 3495
Fragments arranged by size:
3495 896 730 518 57 33
ApaLI G'TGCA_C
Cuts at: 4499 4499
Size: 5729
ApoI r'AATT_y
Cuts at: 342 2733 5082 5338 342
Size: 2391 2349 256 733
Fragments arranged by size:
2391 2349 733 256
AscI GG'CGCG_CC
Cuts at: 197 197
Size: 5729
AseI AT'TA_AT
Cuts at: 20 20
Size: 5729
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5729
AvaI C'yCGr_G
Cuts at: 147 212 1448 1525 1707 1948 1975 2421 2437
Size: 65 1236 77 182 241 27 446 16
Cuts at: 2437 2749 147
Size: 312 3127
Fragments arranged by size:
3127 1236 446 312 241 182 77 65
27 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2628 2661 2628
Size: 33 5696
BamHI G'GATC_C
Cuts at: 2754 2754
Size: 5729
BanI G'GyrC_C
Cuts at: 295 566 754 1545 1606 2106 2214 2293 2580
Size: 271 188 791 61 500 108 79 287
Cuts at: 2580 2745 3159 3407 3442 295
Size: 165 414 248 35 2582
Fragments arranged by size:
2582 791 500 414 287 271 248 188
165 108 79 61 35
BanII G_rGCy'C
Cuts at: 472 483 775 1379 1897 1930 2660 2717 2743
Size: 11 292 604 518 33 730 57 26
Cuts at: 2743 3773 472
Size: 1030 2428
Fragments arranged by size:
2428 1030 730 604 518 292 57 33
26 11
BbeI G_GCGC'C
Cuts at: 299 2110 2218 2584 3163 3411 299
Size: 1811 108 366 579 248 2617
Fragments arranged by size:
2617 1811 579 366 248 108
BbsI GAAGACnn'nnnn_
Cuts at: 1428 2135 1428
Size: 707 5022
BbvCI CC'TCA_GC
Cuts at: 268 1288 268
Size: 1020 4709
BciVI GTATCCnnnnn_n'
Cuts at: 3627 4388 5729 3627
Size: 761 1341 3627
Fragments arranged by size:
3627 1341 761
BclI T'GATC_A
Cuts at: 923 3249 923
Size: 2326 3403
BglI GCCn_nnn'nGGC
Cuts at: 1451 1451
Size: 5729
BglII A'GATC_T
Cuts at: 3244 5281 3244
Size: 2037 3692
BlpI GC'TnA_GC
Cuts at: 2841 2841
Size: 5729
Bme1580I G_kGCm'C
Cuts at: 483 1217 1379 1550 1584 1897 1930 2660 2717
Size: 734 162 171 34 313 33 730 57
Cuts at: 2717 3354 3447 4503 483
Size: 637 93 1056 1709
Fragments arranged by size:
1709 1056 734 730 637 313 171 162
93 57 34 33
BmgBI CAC'GTC
Cuts at: 1797 2465 2700 1797
Size: 668 235 4826
Fragments arranged by size:
4826 668 235
BmrI ACTGGGnnnn_n'
Cuts at: 2526 2941 3022 3357 5501 2526
Size: 415 81 335 2144 2754
Fragments arranged by size:
2754 2144 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 486 1836 4096 486
Size: 1350 2260 2119
Fragments arranged by size:
2260 2119 1350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 597 1158 1236 1300 1425 1578 2166 2521
Size: 215 561 78 64 125 153 588 355
Cuts at: 2521 382
Size: 3590
Fragments arranged by size:
3590 588 561 355 215 153 125 78
64
Bpu10I CC'TnA_GC
Cuts at: 268 1288 268
Size: 1020 4709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 1282 2905 3784 4276 4574 574
Size: 708 1623 879 492 298 1729
Fragments arranged by size:
1729 1623 879 708 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2062 5433 2062
Size: 3371 2358
BsaAI yAC'GTr
Cuts at: 402 1184 3082 402
Size: 782 1898 3049
Fragments arranged by size:
3049 1898 782
BsaBI GATnn'nnATC
Cuts at: 6 622 2053 3243 5280 6
Size: 616 1431 1190 2037 455
Fragments arranged by size:
2037 1431 1190 616 455
BsaHI Gr'CG_yC
Cuts at: 296 1805 2107 2215 2581 3160 3408 4110 5606
Size: 1509 302 108 366 579 248 702 1496
Cuts at: 5606 296
Size: 419
Fragments arranged by size:
1509 1496 702 579 419 366 302 248
108
BsaWI w'CCGG_w
Cuts at: 698 2918 3439 4117 4391 4538 4949 698
Size: 2220 521 678 274 147 411 1478
Fragments arranged by size:
2220 1478 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1162 1192 1234 1264 2281 2302 2311 2332 2605
Size: 30 42 30 1017 21 9 21 273
Cuts at: 2605 2617 2635 2647 1162
Size: 12 18 12 4244
Fragments arranged by size:
4244 1017 273 42 30 30 21 21
18 12 12 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2509 2509
Size: 5729
BseYI C'CCAG_C
Cuts at: 377 768 1507 1537 1896 1957 2705 3154 3912
Size: 391 739 30 359 61 748 449 758
Cuts at: 3912 4489 4985 377
Size: 577 496 1121
Fragments arranged by size:
1121 758 748 739 577 496 449 391
359 61 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 1159 1389 133
Size: 1026 230 4473
Fragments arranged by size:
4473 1026 230
BsiEI CG_ry'CG
Cuts at: 104 370 1934 2225 2359 2576 3271 3317 4525
Size: 266 1564 291 134 217 695 46 1208
Cuts at: 4525 104
Size: 1308
Fragments arranged by size:
1564 1308 1208 695 291 266 217 134
46
BsiHKAI G_wGCw'C
Cuts at: 472 635 2743 3521 3711 4503 472
Size: 163 2108 778 190 792 1698
Fragments arranged by size:
2108 1698 792 778 190 163
BsmBI CGTCTCn'nnnn_
Cuts at: 1352 1352
Size: 5729
Bsp1286I G_dGCh'C
Cuts at: 472 483 635 775 1217 1379 1550 1584 1897
Size: 11 152 140 442 162 171 34 313
Cuts at: 1897 1930 2660 2717 2743 3354 3447 3521 3711
Size: 33 730 57 26 611 93 74 190
Cuts at: 3711 3773 4503 472
Size: 62 730 1698
Fragments arranged by size:
1698 730 730 611 442 313 190 171
162 152 140 93 74 62 57 34
33 26 11
BspEI T'CCGG_A
Cuts at: 698 4949 698
Size: 4251 1478
BspHI T'CATG_A
Cuts at: 4905 4905
Size: 5729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1181 1983 2456 2779 2993 3295 3676 1181
Size: 802 473 323 214 302 381 3234
Fragments arranged by size:
3234 802 473 381 323 302 214
BsrBI CCG'CTC
Cuts at: 50 152 366 2355 4021 4075 5559 5724 50
Size: 102 214 1989 1666 54 1484 165 55
Fragments arranged by size:
1989 1666 1484 214 165 102 55 54
BsrDI GCAATG_nn'
Cuts at: 644 2606 3641 644
Size: 1962 1035 2732
Fragments arranged by size:
2732 1962 1035
BsrFI r'CCGG_y
Cuts at: 235 563 661 743 1825 2103 2677 3727 3908
Size: 328 98 82 1082 278 574 1050 181
Cuts at: 3908 4117 235
Size: 209 1847
Fragments arranged by size:
1847 1082 1050 574 328 278 209 181
98 82
BsrGI T'GTAC_A
Cuts at: 1220 1220
Size: 5729
BssHII G'CGCG_C
Cuts at: 197 197
Size: 5729
BssSI C'ACGA_G
Cuts at: 427 2350 2491 4000 4358 427
Size: 1923 141 1509 358 1798
Fragments arranged by size:
1923 1798 1509 358 141
BstAPI GCAn_nnn'nTGC
Cuts at: 723 1367 3106 723
Size: 644 1739 3346
Fragments arranged by size:
3346 1739 644
BstBI TT'CG_AA
Cuts at: 4090 4090
Size: 5729
BstEII G'GTnAC_C
Cuts at: 1206 1502 1206
Size: 296 5433
BstXI CCAn_nnnn'nTGG
Cuts at: 1965 1965
Size: 5729
BstYI r'GATC_y
Cuts at: 1 539 2754 3221 3244 3579 3825 4837 4923
Size: 538 2215 467 23 335 246 1012 86
Cuts at: 4923 4935 5281 1
Size: 12 346 449
Fragments arranged by size:
2215 1012 538 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 138 138
Size: 5729
BtgI C'CryG_G
Cuts at: 107 570 2003 2192 2289 2303 2313 2483 2576
Size: 463 1433 189 97 14 10 170 93
Cuts at: 2576 107
Size: 3260
Fragments arranged by size:
3260 1433 463 189 170 97 93 14
10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 621 3679 3860 4023 621
Size: 3058 181 163 2327
Fragments arranged by size:
3058 2327 181 163
BtsI GCAGTG_nn'
Cuts at: 806 2972 806
Size: 2166 3563
DraI TTT'AAA
Cuts at: 2728 2728
Size: 5729
DrdI GACnn_nn'nnGTC
Cuts at: 3435 4293 3435
Size: 858 4871
EaeI y'GGCC_r
Cuts at: 367 656 1827 1931 2222 2573 2672 3314 3488
Size: 289 1171 104 291 351 99 642 174
Cuts at: 3488 3879 3906 5251 367
Size: 391 27 1345 845
Fragments arranged by size:
1345 1171 845 642 391 351 291 289
174 104 99 27
EagI C'GGCC_G
Cuts at: 367 1931 2222 2573 3314 367
Size: 1564 291 351 741 2782
Fragments arranged by size:
2782 1564 741 351 291
EarI CTCTTCn'nnn_
Cuts at: 460 1093 3752 3962 460
Size: 633 2659 210 2227
Fragments arranged by size:
2659 2227 633 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1119 1954 2234 2355 3937 4247 4393 5539 1119
Size: 835 280 121 1582 310 146 1146 1309
Fragments arranged by size:
1582 1309 1146 835 310 280 146 121
EcoICRI GAG'CTC
Cuts at: 470 2741 470
Size: 2271 3458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 382 597 1081 1158 1236 1300 1425 1578
Size: 195 215 484 77 78 64 125 153
Cuts at: 1578 2166 2496 2505 2521 3554 3986 4733 5001
Size: 588 330 9 16 1033 432 747 268
Cuts at: 5001 187
Size: 915
Fragments arranged by size:
1033 915 747 588 484 432 330 268
215 195 153 125 78 77 64 16
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2407 2407
Size: 5729
EcoO109I rG'GnC_Cy
Cuts at: 246 479 480 918 1247 1329 1375 1376 1475
Size: 233 1 438 329 82 46 1 99
Cuts at: 1475 1698 1893 1926 1953 2432 2600 2657 2686
Size: 223 195 33 27 479 168 57 29
Cuts at: 2686 2868 246
Size: 182 3107
Fragments arranged by size:
3107 479 438 329 233 223 195 182
168 99 82 57 46 33 29 27
1 1
EcoRI G'AATT_C
Cuts at: 2733 2733
Size: 5729
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 192 2013 2037 3748 192
Size: 1821 24 1711 2173
Fragments arranged by size:
2173 1821 1711 24
FspI TGC'GCA
Cuts at: 2329 3510 2329
Size: 1181 4548
FspAI rTGC'GCAy
Cuts at: 2329 2329
Size: 5729
HaeII r_GCGC'y
Cuts at: 226 299 1514 2110 2218 2584 3163 3411 4433
Size: 73 1215 596 108 366 579 248 1022
Cuts at: 4433 5412 226
Size: 979 543
Fragments arranged by size:
1215 1022 979 596 579 543 366 248
108 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1162 1194 1828 1860 2302 2334 2605 2617 2637
Size: 32 634 32 442 32 271 12 20
Cuts at: 2637 2649 3571 3601 3603 3633 1162
Size: 12 922 30 2 30 3258
Fragments arranged by size:
3258 922 634 442 271 32 32 32
30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 2148 2768 2148
Size: 620 5109
KasI G'GCGC_C
Cuts at: 295 2106 2214 2580 3159 3407 295
Size: 1811 108 366 579 248 2617
Fragments arranged by size:
2617 1811 579 366 248 108
KpnI G_GTAC'C
Cuts at: 570 2749 570
Size: 2179 3550
MluI A'CGCG_T
Cuts at: 5162 5162
Size: 5729
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2660 4400 4584 2660
Size: 1740 184 3805
Fragments arranged by size:
3805 1740 184
MscI TGG'CCA
Cuts at: 3490 3490
Size: 5729
MslI CAynn'nnrTG
Cuts at: 628 649 912 3718 3845 628
Size: 21 263 2806 127 2512
Fragments arranged by size:
2806 2512 263 127 21
MspA1I CmG'CkG
Cuts at: 1269 1278 2046 2516 2564 2840 3154 3514 4527
Size: 9 768 470 48 276 314 360 1013
Cuts at: 4527 4772 1269
Size: 245 2226
Fragments arranged by size:
2226 1013 768 470 360 314 276 245
48 9
NaeI GCC'GGC
Cuts at: 237 663 1827 2105 3910 237
Size: 426 1164 278 1805 2056
Fragments arranged by size:
2056 1805 1164 426 278
NarI GG'CG_CC
Cuts at: 296 2107 2215 2581 3160 3408 296
Size: 1811 108 366 579 248 2617
Fragments arranged by size:
2617 1811 579 366 248 108
NcoI C'CATG_G
Cuts at: 107 2003 107
Size: 1896 3833
NgoMIV G'CCGG_C
Cuts at: 235 661 1825 2103 3908 235
Size: 426 1164 278 1805 2056
Fragments arranged by size:
2056 1805 1164 426 278
NotI GC'GGCC_GC
Cuts at: 367 367
Size: 5729
NspI r_CATG'y
Cuts at: 1842 2043 2146 2782 3282 4189 1842
Size: 201 103 636 500 907 3382
Fragments arranged by size:
3382 907 636 500 201 103
PasI CC'CwG_GG
Cuts at: 669 669
Size: 5729
PciI A'CATG_T
Cuts at: 4185 4185
Size: 5729
PflMI CCAn_nnn'nTGG
Cuts at: 1556 1884 2258 1556
Size: 328 374 5027
Fragments arranged by size:
5027 374 328
PfoI T'CCnGG_A
Cuts at: 491 491
Size: 5729
PmeI GTTT'AAAC
Cuts at: 2728 2728
Size: 5729
PmlI CAC'GTG
Cuts at: 1184 1184
Size: 5729
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4868 4900 4868
Size: 32 5697
PpuMI rG'GwC_Cy
Cuts at: 1329 1475 1698 1953 2686 1329
Size: 146 223 255 733 4372
Fragments arranged by size:
4372 733 255 223 146
PspOMI G'GGCC_C
Cuts at: 479 1375 1893 1926 2656 2713 479
Size: 896 518 33 730 57 3495
Fragments arranged by size:
3495 896 730 518 57 33
PspXI vC'TCGA_Gb
Cuts at: 1525 1525
Size: 5729
PstI C_TGCA'G
Cuts at: 341 719 1035 1865 2431 2776 3461 341
Size: 378 316 830 566 345 685 2609
Fragments arranged by size:
2609 830 685 566 378 345 316
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5729
PvuII CAG'CTG
Cuts at: 1278 2564 3154 3514 1278
Size: 1286 590 360 3493
Fragments arranged by size:
3493 1286 590 360
RsrII CG'GwC_CG
Cuts at: 3924 3924
Size: 5729
SacI G_AGCT'C
Cuts at: 472 2743 472
Size: 2271 3458
SalI G'TCGA_C
Cuts at: 2146 2766 2146
Size: 620 5109
SanDI GG'GwC_CC
Cuts at: 1475 2686 1475
Size: 1211 4518
SapI GCTCTTCn'nnn_
Cuts at: 460 3752 3962 460
Size: 3292 210 2227
Fragments arranged by size:
3292 2227 210
SbfI CC_TGCA'GG
Cuts at: 1035 2776 1035
Size: 1741 3988
ScaI AGT'ACT
Cuts at: 529 1192 1819 5246 529
Size: 663 627 3427 1012
Fragments arranged by size:
3427 1012 663 627
SfcI C'TryA_G
Cuts at: 33 337 715 1031 1861 2427 2772 3457 4450
Size: 304 378 316 830 566 345 685 993
Cuts at: 4450 4641 33
Size: 191 1121
Fragments arranged by size:
1121 993 830 685 566 378 345 316
304 191
SfoI GGC'GCC
Cuts at: 297 2108 2216 2582 3161 3409 297
Size: 1811 108 366 579 248 2617
Fragments arranged by size:
2617 1811 579 366 248 108
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5729
SgrAI Cr'CCGG_yG
Cuts at: 2677 2677
Size: 5729
SmaI CCC'GGG
Cuts at: 214 1709 2423 2751 214
Size: 1495 714 328 3192
Fragments arranged by size:
3192 1495 714 328
SmlI C'TyrA_G
Cuts at: 589 1297 1525 2884 3799 4291 4553 589
Size: 708 228 1359 915 492 262 1765
Fragments arranged by size:
1765 1359 915 708 492 262 228
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5729
SphI G_CATG'C
Cuts at: 2782 3282 2782
Size: 500 5229
StuI AGG'CCT
Cuts at: 1121 2077 1121
Size: 956 4773
StyI C'CwwG_G
Cuts at: 107 1463 1922 2003 2537 2863 3217 107
Size: 1356 459 81 534 326 354 2619
Fragments arranged by size:
2619 1356 534 459 354 326 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1521 1708 2086 3312 3445 3904 4113 1521
Size: 187 378 1226 133 459 209 3137
Fragments arranged by size:
3137 1226 459 378 209 187 133
TatI w'GTAC_w
Cuts at: 527 857 1190 1220 1817 5244 527
Size: 330 333 30 597 3427 1012
Fragments arranged by size:
3427 1012 597 333 330 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2281 2313 5246 5278 2281
Size: 32 2933 32 2732
Fragments arranged by size:
2933 2732 32 32
Tth111I GACn'n_nGTC
Cuts at: 3526 3526
Size: 5729
XbaI T'CTAG_A
Cuts at: 2760 2760
Size: 5729
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 651 2481 2544 3224 651
Size: 1830 63 680 3156
Fragments arranged by size:
3156 1830 680 63
XhoI C'TCGA_G
Cuts at: 1525 1525
Size: 5729
XmaI C'CCGG_G
Cuts at: 212 1707 2421 2749 212
Size: 1495 714 328 3192
Fragments arranged by size:
3192 1495 714 328
XmnI GAAnn'nnTTC
Cuts at: 607 2206 607
Size: 1599 4130
ZraI GAC'GTC
Cuts at: 1806 1806
Size: 5729
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI AvrII BcgI BfrBI BmtI
BsiWI BsmI Bsu36I ClaI CspCI DraIII EcoRV FseI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PshAI PsiI PsrI SacII SexAI SfiI SnaBI SrfI
SspI SwaI