Restriction map for
pF1KB7331
(Circular) (Six-base) MAPSORT of: pF1KB7331.seq Check: 9010 from: 1 to: 6095
pF1KB7331 6095 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 411 1484 2551 2859 411
Size: 1073 1067 308 3647
Fragments arranged by size:
3647 1073 1067 308
AccI GT'mk_AC
Cuts at: 938 2939 3133 938
Size: 2001 194 3900
Fragments arranged by size:
3900 2001 194
Acc65I G'GTAC_C
Cuts at: 492 1762 3111 492
Size: 1270 1349 3476
Fragments arranged by size:
3476 1349 1270
AclI AA'CG_TT
Cuts at: 5935 5935
Size: 6095
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 1294 1716 2743 3920 4352 5099 5367 743
Size: 551 422 1027 1177 432 747 268 1471
Fragments arranged by size:
1471 1177 1027 747 551 432 422 268
AflIII A'CryG_T
Cuts at: 2233 3447 4551 5528 2233
Size: 1214 1104 977 2800
Fragments arranged by size:
2800 1214 1104 977
AgeI A'CCGG_T
Cuts at: 4483 4483
Size: 6095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 610 1687 610
Size: 1077 5018
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 254 286 254
Size: 32 6063
AlwNI CAG_nnn'CTG
Cuts at: 647 1003 3400 4967 647
Size: 356 2397 1567 1775
Fragments arranged by size:
2397 1775 1567 356
ApaI G_GGCC'C
Cuts at: 584 2102 2589 584
Size: 1518 487 4090
Fragments arranged by size:
4090 1518 487
ApaLI G'TGCA_C
Cuts at: 2293 4865 2293
Size: 2572 3523
ApoI r'AATT_y
Cuts at: 331 846 3099 5448 5704 331
Size: 515 2253 2349 256 722
Fragments arranged by size:
2349 2253 722 515 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6095
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6095
AvaI C'yCGr_G
Cuts at: 1407 2075 2164 3115 1407
Size: 668 89 951 4387
Fragments arranged by size:
4387 951 668 89
AvrII C'CTAG_G
Cuts at: 603 603
Size: 6095
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1754 1787 1754
Size: 33 6062
BamHI G'GATC_C
Cuts at: 728 2344 3120 728
Size: 1616 776 3703
Fragments arranged by size:
3703 1616 776
BanI G'GyrC_C
Cuts at: 492 1138 1279 1762 2217 2299 2647 2756 2847
Size: 646 141 483 455 82 348 109 91
Cuts at: 2847 3111 3525 3773 3808 492
Size: 264 414 248 35 2779
Fragments arranged by size:
2779 646 483 455 414 348 264 248
141 109 91 82 35
BanII G_rGCy'C
Cuts at: 584 868 1337 1634 1712 1813 1943 2102 2275
Size: 284 469 297 78 101 130 159 173
Cuts at: 2275 2589 3109 4139 584
Size: 314 520 1030 2540
Fragments arranged by size:
2540 1030 520 469 314 297 284 173
159 130 101 78
BbeI G_GCGC'C
Cuts at: 1283 3529 3777 1283
Size: 2246 248 3601
Fragments arranged by size:
3601 2246 248
BbvCI CC'TCA_GC
Cuts at: 370 889 370
Size: 519 5576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1865 1899 90
Size: 34 1741 34 4286
Fragments arranged by size:
4286 1741 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3993 4754 6095 3993
Size: 761 1341 3993
Fragments arranged by size:
3993 1341 761
BclI T'GATC_A
Cuts at: 3615 3615
Size: 6095
BglI GCCn_nnn'nGGC
Cuts at: 863 2561 863
Size: 1698 4397
BglII A'GATC_T
Cuts at: 3610 5647 3610
Size: 2037 4058
BlpI GC'TnA_GC
Cuts at: 1603 3207 1603
Size: 1604 4491
Bme1580I G_kGCm'C
Cuts at: 584 599 1245 1357 2102 2222 2297 2589 2637
Size: 15 646 112 745 120 75 292 48
Cuts at: 2637 2652 2709 2759 3720 3813 4869 584
Size: 15 57 50 961 93 1056 1810
Fragments arranged by size:
1810 1056 961 745 646 292 120 112
93 75 57 50 48 15 15
BmrI ACTGGGnnnn_n'
Cuts at: 501 1842 3307 3388 3723 5867 501
Size: 1341 1465 81 335 2144 729
Fragments arranged by size:
2144 1465 1341 729 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1827 4462 1827
Size: 2635 3460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 546 560 692 1382 1822 2450 2844 2937
Size: 85 14 132 690 440 628 394 93
Cuts at: 2937 2944 461
Size: 7 3612
Fragments arranged by size:
3612 690 628 440 394 132 93 85
14 7
Bpu10I CC'TnA_GC
Cuts at: 251 370 889 1171 2285 251
Size: 119 519 282 1114 4061
Fragments arranged by size:
4061 1114 519 282 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1468 2596 3271 4150 4642 4940 346
Size: 1122 1128 675 879 492 298 1501
Fragments arranged by size:
1501 1128 1122 879 675 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 769 2632 5799 769
Size: 1863 3167 1065
Fragments arranged by size:
3167 1863 1065
BsaAI yAC'GTr
Cuts at: 3448 3448
Size: 6095
BsaBI GATnn'nnATC
Cuts at: 6 727 3609 5646 6
Size: 721 2882 2037 455
Fragments arranged by size:
2882 2037 721 455
BsaHI Gr'CG_yC
Cuts at: 269 1280 2451 3526 3774 4476 5972 269
Size: 1011 1171 1075 248 702 1496 392
Fragments arranged by size:
1496 1171 1075 1011 702 392 248
BsaWI w'CCGG_w
Cuts at: 531 2238 3284 3805 4483 4757 4904 5315 531
Size: 1707 1046 521 678 274 147 411 1311
Fragments arranged by size:
1707 1311 1046 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 526 556 877 907 526
Size: 30 321 30 5714
Fragments arranged by size:
5714 321 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 379 738 787 229
Size: 150 359 49 5537
Fragments arranged by size:
5537 359 150 49
BseYI C'CCAG_C
Cuts at: 583 637 1310 2178 2676 3520 4278 4855 5351
Size: 54 673 868 498 844 758 577 496
Cuts at: 5351 583
Size: 1327
Fragments arranged by size:
1327 868 844 758 673 577 498 496
54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1462 1905 389
Size: 1073 443 4579
Fragments arranged by size:
4579 1073 443
BsiEI CG_ry'CG
Cuts at: 104 1386 1950 3637 3683 4891 104
Size: 1282 564 1687 46 1208 1308
Fragments arranged by size:
1687 1308 1282 1208 564 46
BsiHKAI G_wGCw'C
Cuts at: 1634 2297 2444 3109 3887 4077 4869 1634
Size: 663 147 665 778 190 792 2860
Fragments arranged by size:
2860 792 778 665 663 190 147
BsmBI CGTCTCn'nnnn_
Cuts at: 1398 3071 1398
Size: 1673 4422
Bsp1286I G_dGCh'C
Cuts at: 584 599 868 1245 1337 1357 1634 1712 1813
Size: 15 269 377 92 20 277 78 101
Cuts at: 1813 1943 2102 2222 2275 2297 2444 2589 2637
Size: 130 159 120 53 22 147 145 48
Cuts at: 2637 2652 2709 2759 3109 3720 3813 3887 4077
Size: 15 57 50 350 611 93 74 190
Cuts at: 4077 4139 4869 584
Size: 62 730 1810
Fragments arranged by size:
1810 730 611 377 350 277 269 190
159 147 145 130 120 101 93 92
78 74 62 57 53 50 48 22
20 15 15
BspEI T'CCGG_A
Cuts at: 531 2238 5315 531
Size: 1707 3077 1311
Fragments arranged by size:
3077 1707 1311
BspHI T'CATG_A
Cuts at: 5271 5271
Size: 6095
BspMI ACCTGCnnnn'nnnn_
Cuts at: 411 1484 1592 2399 2551 2859 3145 3359 3661
Size: 1073 108 807 152 308 286 214 302
Cuts at: 3661 4042 411
Size: 381 2464
Fragments arranged by size:
2464 1073 807 381 308 302 286 214
152 108
BsrBI CCG'CTC
Cuts at: 50 4387 4441 5925 6090 50
Size: 4337 54 1484 165 55
Fragments arranged by size:
4337 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1377 1536 1698 2232 2882 4007 1377
Size: 159 162 534 650 1125 3465
Fragments arranged by size:
3465 1125 650 534 162 159
BsrFI r'CCGG_y
Cuts at: 1125 1381 4093 4274 4483 1125
Size: 256 2712 181 209 2737
Fragments arranged by size:
2737 2712 256 209 181
BssSI C'ACGA_G
Cuts at: 2700 4366 4724 2700
Size: 1666 358 4071
Fragments arranged by size:
4071 1666 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2854 3472 2854
Size: 618 5477
BstBI TT'CG_AA
Cuts at: 555 4456 555
Size: 3901 2194
BstXI CCAn_nnnn'nTGG
Cuts at: 2428 2868 2428
Size: 440 5655
BstYI r'GATC_y
Cuts at: 1 728 2252 2344 3058 3120 3587 3610 3945
Size: 727 1524 92 714 62 467 23 335
Cuts at: 3945 4191 5203 5289 5301 5647 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1524 1012 727 714 467 449 346 335
246 92 86 62 23 12
BstZ17I GTA'TAC
Cuts at: 2940 2940
Size: 6095
Bsu36I CC'TnA_GG
Cuts at: 1102 1457 1102
Size: 355 5740
BtgI C'CryG_G
Cuts at: 185 482 688 2129 2303 2477 2622 185
Size: 297 206 1441 174 174 145 3658
Fragments arranged by size:
3658 1441 297 206 174 174 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2261 2929 4045 4226 4389 2261
Size: 668 1116 181 163 3967
Fragments arranged by size:
3967 1116 668 181 163
BtsI GCAGTG_nn'
Cuts at: 379 599 1030 3338 379
Size: 220 431 2308 3136
Fragments arranged by size:
3136 2308 431 220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 888 923 888
Size: 35 6060
DraI TTT'AAA
Cuts at: 3094 3094
Size: 6095
DrdI GACnn_nn'nnGTC
Cuts at: 527 3801 4659 527
Size: 3274 858 1963
Fragments arranged by size:
3274 1963 858
EaeI y'GGCC_r
Cuts at: 437 1040 1383 1933 3680 3854 4245 4272 5617
Size: 603 343 550 1747 174 391 27 1345
Cuts at: 5617 437
Size: 915
Fragments arranged by size:
1747 1345 915 603 550 391 343 174
27
EagI C'GGCC_G
Cuts at: 1383 3680 1383
Size: 2297 3798
EarI CTCTTCn'nnn_
Cuts at: 1979 2263 4118 4328 1979
Size: 284 1855 210 3746
Fragments arranged by size:
3746 1855 284 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4303 4613 4759 5905 4303
Size: 310 146 1146 4493
Fragments arranged by size:
4493 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1632 3107 1632
Size: 1475 4620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 546 560 692 743 1294 1382 1716 1822
Size: 85 14 132 51 551 88 334 106
Cuts at: 1822 2450 2743 2844 2937 2944 3920 4352 5099
Size: 628 293 101 93 7 976 432 747
Cuts at: 5099 5367 461
Size: 268 1189
Fragments arranged by size:
1189 976 747 628 551 432 334 293
268 132 106 101 93 88 85 51
14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 340 1501 1804 340
Size: 1161 303 4631
Fragments arranged by size:
4631 1161 303
EcoO109I rG'GnC_Cy
Cuts at: 177 520 580 667 832 1021 1548 1596 2029
Size: 343 60 87 165 189 527 48 433
Cuts at: 2029 2098 2099 2168 2370 2388 2586 3234 177
Size: 69 1 69 202 18 198 648 3038
Fragments arranged by size:
3038 648 527 433 343 202 198 189
165 87 69 69 60 48 18 1
EcoRI G'AATT_C
Cuts at: 331 3099 331
Size: 2768 3327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4114 4114
Size: 6095
FspI TGC'GCA
Cuts at: 2023 3876 2023
Size: 1853 4242
HaeII r_GCGC'y
Cuts at: 151 1283 3529 3777 4799 5778 151
Size: 1132 2246 248 1022 979 468
Fragments arranged by size:
2246 1132 1022 979 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 526 558 691 723 877 909 2336 2368 2380
Size: 32 133 32 154 32 1427 32 12
Cuts at: 2380 2412 3043 3045 3075 3077 3937 3967 3969
Size: 32 631 2 30 2 860 30 2
Cuts at: 3969 3999 526
Size: 30 2622
Fragments arranged by size:
2622 1427 860 631 154 133 32 32
32 32 32 30 30 30 12 2
2 2
HincII GTy'rAC
Cuts at: 1833 3134 1833
Size: 1301 4794
KasI G'GCGC_C
Cuts at: 1279 3525 3773 1279
Size: 2246 248 3601
Fragments arranged by size:
3601 2246 248
KpnI G_GTAC'C
Cuts at: 496 1766 3115 496
Size: 1270 1349 3476
Fragments arranged by size:
3476 1349 1270
MluI A'CGCG_T
Cuts at: 5528 5528
Size: 6095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2138 3067 4766 4950 2138
Size: 929 1699 184 3283
Fragments arranged by size:
3283 1699 929 184
MscI TGG'CCA
Cuts at: 439 1042 1935 3856 439
Size: 603 893 1921 2678
Fragments arranged by size:
2678 1921 893 603
MslI CAynn'nnrTG
Cuts at: 592 1703 2014 2771 4084 4211 592
Size: 1111 311 757 1313 127 2476
Fragments arranged by size:
2476 1313 1111 757 311 127
MspA1I CmG'CkG
Cuts at: 321 1272 1467 1649 1657 1781 1889 2890 2982
Size: 951 195 182 8 124 108 1001 92
Cuts at: 2982 3206 3520 3880 4893 5138 321
Size: 224 314 360 1013 245 1278
Fragments arranged by size:
1278 1013 1001 951 360 314 245 224
195 182 124 108 92 8
NaeI GCC'GGC
Cuts at: 4276 4276
Size: 6095
NarI GG'CG_CC
Cuts at: 1280 3526 3774 1280
Size: 2246 248 3601
Fragments arranged by size:
3601 2246 248
NcoI C'CATG_G
Cuts at: 482 2129 2622 482
Size: 1647 493 3955
Fragments arranged by size:
3955 1647 493
NgoMIV G'CCGG_C
Cuts at: 4274 4274
Size: 6095
NspI r_CATG'y
Cuts at: 1981 2237 3148 3648 4555 1981
Size: 256 911 500 907 3521
Fragments arranged by size:
3521 911 907 500 256
PasI CC'CwG_GG
Cuts at: 610 711 1231 1357 610
Size: 101 520 126 5348
Fragments arranged by size:
5348 520 126 101
PciI A'CATG_T
Cuts at: 2233 4551 2233
Size: 2318 3777
PflMI CCAn_nnn'nTGG
Cuts at: 1129 1201 2264 2340 2623 1129
Size: 72 1063 76 283 4601
Fragments arranged by size:
4601 1063 283 76 72
PfoI T'CCnGG_A
Cuts at: 669 983 2427 2600 669
Size: 314 1444 173 4164
Fragments arranged by size:
4164 1444 314 173
PmeI GTTT'AAAC
Cuts at: 3094 3094
Size: 6095
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5234 5266 5234
Size: 32 6063
PpuMI rG'GwC_Cy
Cuts at: 520 667 2168 2370 2388 520
Size: 147 1501 202 18 4227
Fragments arranged by size:
4227 1501 202 147 18
PshAI GACnn'nnGTC
Cuts at: 2234 2234
Size: 6095
PspOMI G'GGCC_C
Cuts at: 580 2098 2585 580
Size: 1518 487 4090
Fragments arranged by size:
4090 1518 487
PstI C_TGCA'G
Cuts at: 1662 2981 3142 3827 1662
Size: 1319 161 685 3930
Fragments arranged by size:
3930 1319 685 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6095
PvuII CAG'CTG
Cuts at: 1272 1657 1781 2890 2982 3520 3880 1272
Size: 385 124 1109 92 538 360 3487
Fragments arranged by size:
3487 1109 538 385 360 124 92
RsrII CG'GwC_CG
Cuts at: 4290 4290
Size: 6095
SacI G_AGCT'C
Cuts at: 1634 3109 1634
Size: 1475 4620
SalI G'TCGA_C
Cuts at: 3132 3132
Size: 6095
SanDI GG'GwC_CC
Cuts at: 520 520
Size: 6095
SapI GCTCTTCn'nnn_
Cuts at: 2263 4118 4328 2263
Size: 1855 210 4030
Fragments arranged by size:
4030 1855 210
SbfI CC_TGCA'GG
Cuts at: 3142 3142
Size: 6095
ScaI AGT'ACT
Cuts at: 1638 2092 5612 1638
Size: 454 3520 2121
Fragments arranged by size:
3520 2121 454
SexAI A'CCwGG_T
Cuts at: 2333 2333
Size: 6095
SfcI C'TryA_G
Cuts at: 33 881 1416 1658 2790 2977 3138 3823 4816
Size: 848 535 242 1132 187 161 685 993
Cuts at: 4816 5007 33
Size: 191 1121
Fragments arranged by size:
1132 1121 993 848 685 535 242 191
187 161
SfoI GGC'GCC
Cuts at: 1281 3527 3775 1281
Size: 2246 248 3601
Fragments arranged by size:
3601 2246 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6095
SmaI CCC'GGG
Cuts at: 1409 2077 2166 3117 1409
Size: 668 89 951 4387
Fragments arranged by size:
4387 951 668 89
SmlI C'TyrA_G
Cuts at: 325 1447 2611 3250 4165 4657 4919 325
Size: 1122 1164 639 915 492 262 1501
Fragments arranged by size:
1501 1164 1122 915 639 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6095
SphI G_CATG'C
Cuts at: 3148 3648 3148
Size: 500 5595
StuI AGG'CCT
Cuts at: 1013 1684 1013
Size: 671 5424
StyI C'CwwG_G
Cuts at: 482 512 603 2129 2622 2843 3229 3583 482
Size: 30 91 1526 493 221 386 354 2994
Fragments arranged by size:
2994 1526 493 386 354 221 91 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1102 1244 1324 1761 2690 2799 3678 3811 4270
Size: 142 80 437 929 109 879 133 459
Cuts at: 4270 4479 1102
Size: 209 2718
Fragments arranged by size:
2718 929 879 459 437 209 142 133
109 80
TatI w'GTAC_w
Cuts at: 1636 2090 5610 1636
Size: 454 3520 2121
Fragments arranged by size:
3520 2121 454
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 371 403 405 437 5612 5644 371
Size: 32 2 32 5175 32 822
Fragments arranged by size:
5175 822 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 3892 3892
Size: 6095
XbaI T'CTAG_A
Cuts at: 3126 3126
Size: 6095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 480 667 1258 1755 2323 2475 3590 480
Size: 187 591 497 568 152 1115 2985
Fragments arranged by size:
2985 1115 591 568 497 187 152
XmaI C'CCGG_G
Cuts at: 1407 2075 2164 3115 1407
Size: 668 89 951 4387
Fragments arranged by size:
4387 951 668 89
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI BbsI BfrBI
BmgBI BmtI BsiWI BsmI BsrGI BssHII BstEII ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI