Restriction map for
pF1KB7334
(Circular) (Six-base) MAPSORT of: pF1KB7334.seq Check: 7928 from: 1 to: 7436
pF1KB7334 7436 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 281 281
Size: 7436
AccI GT'mk_AC
Cuts at: 2099 3546 4474 2099
Size: 1447 928 5061
Fragments arranged by size:
5061 1447 928
Acc65I G'GTAC_C
Cuts at: 599 4452 599
Size: 3853 3583
AclI AA'CG_TT
Cuts at: 7276 7276
Size: 7436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1232 2264 2711 3098 3663 3902 5261 5693 6440
Size: 1032 447 387 565 239 1359 432 747
Cuts at: 6440 6708 1232
Size: 268 1960
Fragments arranged by size:
1960 1359 1032 747 565 447 432 387
268 239
AfeI AGC'GCT
Cuts at: 202 202
Size: 7436
AflIII A'CryG_T
Cuts at: 2656 4788 5892 6869 2656
Size: 2132 1104 977 3223
Fragments arranged by size:
3223 2132 1104 977
AgeI A'CCGG_T
Cuts at: 5824 5824
Size: 7436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3607 3607
Size: 7436
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2113 2145 2113
Size: 32 7404
AlwNI CAG_nnn'CTG
Cuts at: 410 3352 4741 6308 410
Size: 2942 1389 1567 1538
Fragments arranged by size:
2942 1567 1538 1389
ApaLI G'TGCA_C
Cuts at: 6206 6206
Size: 7436
ApoI r'AATT_y
Cuts at: 514 1162 1467 1578 2221 2570 2598 3430 4440
Size: 648 305 111 643 349 28 832 1010
Cuts at: 4440 6789 7045 514
Size: 2349 256 905
Fragments arranged by size:
2349 1010 905 832 648 643 349 305
256 111 28
AseI AT'TA_AT
Cuts at: 20 530 20
Size: 510 6926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7436
AvaI C'yCGr_G
Cuts at: 156 779 4456 156
Size: 623 3677 3136
Fragments arranged by size:
3677 3136 623
AvrII C'CTAG_G
Cuts at: 4087 4087
Size: 7436
BamHI G'GATC_C
Cuts at: 4461 4461
Size: 7436
BanI G'GyrC_C
Cuts at: 264 599 4452 4866 5114 5149 264
Size: 335 3853 414 248 35 2551
Fragments arranged by size:
3853 2551 414 335 248 35
BanII G_rGCy'C
Cuts at: 317 4186 4234 4450 5480 317
Size: 3869 48 216 1030 2273
Fragments arranged by size:
3869 2273 1030 216 48
BbeI G_GCGC'C
Cuts at: 268 4870 5118 268
Size: 4602 248 2586
Fragments arranged by size:
4602 2586 248
BbsI GAAGACnn'nnnn_
Cuts at: 2395 3770 3840 2395
Size: 1375 70 5991
Fragments arranged by size:
5991 1375 70
BciVI GTATCCnnnnn_n'
Cuts at: 2845 5334 6095 7436 2845
Size: 2489 761 1341 2845
Fragments arranged by size:
2845 2489 1341 761
BclI T'GATC_A
Cuts at: 380 651 2885 4956 380
Size: 271 2234 2071 2860
Fragments arranged by size:
2860 2234 2071 271
BfrBI ATG'CAT
Cuts at: 677 677
Size: 7436
BglI GCCn_nnn'nGGC
Cuts at: 3576 3576
Size: 7436
BglII A'GATC_T
Cuts at: 1752 2437 4951 6988 1752
Size: 685 2514 2037 2200
Fragments arranged by size:
2514 2200 2037 685
BlpI GC'TnA_GC
Cuts at: 4548 4548
Size: 7436
Bme1580I G_kGCm'C
Cuts at: 169 5061 5154 6210 169
Size: 4892 93 1056 1395
Fragments arranged by size:
4892 1395 1056 93
BmgBI CAC'GTC
Cuts at: 3802 3802
Size: 7436
BmrI ACTGGGnnnn_n'
Cuts at: 4648 4729 5064 7208 4648
Size: 81 335 2144 4876
Fragments arranged by size:
4876 2144 335 81
BmtI G_CTAG'C
Cuts at: 3898 3898
Size: 7436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2298 5803 2298
Size: 3505 3931
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 544 1694 268
Size: 276 1150 6010
Fragments arranged by size:
6010 1150 276
Bpu10I CC'TnA_GC
Cuts at: 242 2816 242
Size: 2574 4862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1633 3437 4612 5491 5983 6281 1633
Size: 1804 1175 879 492 298 2788
Fragments arranged by size:
2788 1804 1175 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 7140 7140
Size: 7436
BsaAI yAC'GTr
Cuts at: 2659 4789 2659
Size: 2130 5306
BsaBI GATnn'nnATC
Cuts at: 6 506 614 2887 4061 4950 6987 6
Size: 500 108 2273 1174 889 2037 455
Fragments arranged by size:
2273 2037 1174 889 500 455 108
BsaHI Gr'CG_yC
Cuts at: 265 278 4867 5115 5817 7313 265
Size: 13 4589 248 702 1496 388
Fragments arranged by size:
4589 1496 702 388 248 13
BsaWI w'CCGG_w
Cuts at: 4010 4130 4625 5146 5824 6098 6245 6656 4010
Size: 120 495 521 678 274 147 411 4790
Fragments arranged by size:
4790 678 521 495 411 274 147 120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3227 3257 3227
Size: 30 7406
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 134 550 820 889 2197 2419 2749 2867 3251
Size: 416 270 69 1308 222 330 118 384
Cuts at: 3251 3254 3652 3664 3979 3982 3985 4042 4045
Size: 3 398 12 315 3 3 57 3
Cuts at: 4045 4381 134
Size: 336 3189
Fragments arranged by size:
3189 1308 416 398 384 336 330 315
270 222 118 69 57 12 3 3
3 3
BseYI C'CCAG_C
Cuts at: 790 4861 5619 6196 6692 790
Size: 4071 758 577 496 1534
Fragments arranged by size:
4071 1534 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 4978 5024 6232 104
Size: 4874 46 1208 1308
Fragments arranged by size:
4874 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 317 4117 4359 4450 5228 5418 6210 317
Size: 3800 242 91 778 190 792 1543
Fragments arranged by size:
3800 1543 792 778 242 190 91
BsmI GAATG_Cn'
Cuts at: 463 2059 463
Size: 1596 5840
BsmBI CGTCTCn'nnnn_
Cuts at: 562 4012 562
Size: 3450 3986
Bsp1286I G_dGCh'C
Cuts at: 169 317 4117 4186 4234 4359 4450 5061 5154
Size: 148 3800 69 48 125 91 611 93
Cuts at: 5154 5228 5418 5480 6210 169
Size: 74 190 62 730 1395
Fragments arranged by size:
3800 1395 730 611 190 148 125 93
91 74 69 62 48
BspEI T'CCGG_A
Cuts at: 6656 6656
Size: 7436
BspHI T'CATG_A
Cuts at: 1413 6612 1413
Size: 5199 2237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 4486 4700 5002 5383 230
Size: 4256 214 302 381 2283
Fragments arranged by size:
4256 2283 381 302 214
BsrBI CCG'CTC
Cuts at: 50 252 5728 5782 7266 7431 50
Size: 202 5476 54 1484 165 55
Fragments arranged by size:
5476 1484 202 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2688 5348 2688
Size: 2660 4776
BsrFI r'CCGG_y
Cuts at: 3371 5434 5615 5824 3371
Size: 2063 181 209 4983
Fragments arranged by size:
4983 2063 209 181
BsrGI T'GTAC_A
Cuts at: 632 632
Size: 7436
BssSI C'ACGA_G
Cuts at: 5707 6065 5707
Size: 358 7078
BstAPI GCAn_nnn'nTGC
Cuts at: 3352 4813 3352
Size: 1461 5975
BstBI TT'CG_AA
Cuts at: 3921 5797 3921
Size: 1876 5560
BstXI CCAn_nnnn'nTGG
Cuts at: 568 2173 568
Size: 1605 5831
BstYI r'GATC_y
Cuts at: 1 1101 1388 1670 1752 2437 3448 3501 4461
Size: 1100 287 282 82 685 1011 53 960
Cuts at: 4461 4928 4951 5286 5532 6544 6630 6642 6988
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6988 1
Size: 449
Fragments arranged by size:
1100 1012 1011 960 685 467 449 346
335 287 282 246 86 82 53 23
12
Bsu36I CC'TnA_GG
Cuts at: 1976 1976
Size: 7436
BtgI C'CryG_G
Cuts at: 107 107
Size: 7436
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5386 5567 5730 5386
Size: 181 163 7092
Fragments arranged by size:
7092 181 163
BtsI GCAGTG_nn'
Cuts at: 1400 2255 2459 2712 4679 1400
Size: 855 204 253 1967 4157
Fragments arranged by size:
4157 1967 855 253 204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2778 2813 2778
Size: 35 7401
DraI TTT'AAA
Cuts at: 4435 4435
Size: 7436
DrdI GACnn_nn'nnGTC
Cuts at: 1300 5142 6000 1300
Size: 3842 858 2736
Fragments arranged by size:
3842 2736 858
EaeI y'GGCC_r
Cuts at: 2079 3246 3568 5021 5195 5586 5613 6958 2079
Size: 1167 322 1453 174 391 27 1345 2557
Fragments arranged by size:
2557 1453 1345 1167 391 322 174 27
EagI C'GGCC_G
Cuts at: 5021 5021
Size: 7436
EarI CTCTTCn'nnn_
Cuts at: 669 780 917 1367 1508 1764 1802 2396 2528
Size: 111 137 450 141 256 38 594 132
Cuts at: 2528 2726 3410 3629 3784 3851 3971 4145 4358
Size: 198 684 219 155 67 120 174 213
Cuts at: 4358 5459 5669 669
Size: 1101 210 2436
Fragments arranged by size:
2436 1101 684 594 450 256 219 213
210 198 174 155 141 137 132 120
111 67 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3494 5644 5954 6100 7246 3494
Size: 2150 310 146 1146 3684
Fragments arranged by size:
3684 2150 1146 310 146
EcoICRI GAG'CTC
Cuts at: 315 4448 315
Size: 4133 3303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 544 1232 1694 2264 2711 3098 3663 3902
Size: 276 688 462 570 447 387 565 239
Cuts at: 3902 5261 5693 6440 6708 268
Size: 1359 432 747 268 996
Fragments arranged by size:
1359 996 747 688 570 565 462 447
432 387 276 268 239
EcoNI CCTnn'n_nnAGG
Cuts at: 2116 2286 2116
Size: 170 7266
EcoO109I rG'GnC_Cy
Cuts at: 1309 4092 4575 1309
Size: 2783 483 4170
Fragments arranged by size:
4170 2783 483
EcoRI G'AATT_C
Cuts at: 3430 4440 3430
Size: 1010 6426
EcoRV GAT'ATC
Cuts at: 2324 3261 2324
Size: 937 6499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4396 5455 4396
Size: 1059 6377
FspI TGC'GCA
Cuts at: 5217 5217
Size: 7436
HaeII r_GCGC'y
Cuts at: 204 268 1842 4870 5118 6140 7119 204
Size: 64 1574 3028 248 1022 979 521
Fragments arranged by size:
3028 1574 1022 979 521 248 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 129 161 1662 1694 2313 2345 2469 2501 2634
Size: 32 1501 32 619 32 124 32 133
Cuts at: 2634 2666 3493 3525 3628 3660 5278 5308 5310
Size: 32 827 32 103 32 1618 30 2
Cuts at: 5310 5340 129
Size: 30 2225
Fragments arranged by size:
2225 1618 1501 827 619 133 124 103
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2540 3143 4475 2540
Size: 603 1332 5501
Fragments arranged by size:
5501 1332 603
KasI G'GCGC_C
Cuts at: 264 4866 5114 264
Size: 4602 248 2586
Fragments arranged by size:
4602 2586 248
KpnI G_GTAC'C
Cuts at: 603 4456 603
Size: 3853 3583
MfeI C'AATT_G
Cuts at: 566 604 3194 566
Size: 38 2590 4808
Fragments arranged by size:
4808 2590 38
MluI A'CGCG_T
Cuts at: 6869 6869
Size: 7436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1357 2311 6107 6291 1357
Size: 954 3796 184 2502
Fragments arranged by size:
3796 2502 954 184
MscI TGG'CCA
Cuts at: 2081 5197 2081
Size: 3116 4320
MslI CAynn'nnrTG
Cuts at: 672 4055 5425 5552 672
Size: 3383 1370 127 2556
Fragments arranged by size:
3383 2556 1370 127
MspA1I CmG'CkG
Cuts at: 127 213 1428 1984 3349 4547 4861 5221 6234
Size: 86 1215 556 1365 1198 314 360 1013
Cuts at: 6234 6479 127
Size: 245 1084
Fragments arranged by size:
1365 1215 1198 1084 1013 556 360 314
245 86
NaeI GCC'GGC
Cuts at: 3373 5617 3373
Size: 2244 5192
NarI GG'CG_CC
Cuts at: 265 4867 5115 265
Size: 4602 248 2586
Fragments arranged by size:
4602 2586 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 7436
NgoMIV G'CCGG_C
Cuts at: 3371 5615 3371
Size: 2244 5192
NheI G'CTAG_C
Cuts at: 3894 3894
Size: 7436
NsiI A_TGCA'T
Cuts at: 679 679
Size: 7436
NspI r_CATG'y
Cuts at: 4489 4989 5896 4489
Size: 500 907 6029
Fragments arranged by size:
6029 907 500
PciI A'CATG_T
Cuts at: 5892 5892
Size: 7436
PmeI GTTT'AAAC
Cuts at: 4435 4435
Size: 7436
PmlI CAC'GTG
Cuts at: 2659 2659
Size: 7436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 220 252 6575 6607 220
Size: 32 6323 32 1049
Fragments arranged by size:
6323 1049 32 32
PpuMI rG'GwC_Cy
Cuts at: 1309 4092 1309
Size: 2783 4653
PstI C_TGCA'G
Cuts at: 263 360 2724 4483 5168 263
Size: 97 2364 1759 685 2531
Fragments arranged by size:
2531 2364 1759 685 97
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7436
PvuII CAG'CTG
Cuts at: 1428 1984 3349 4861 5221 1428
Size: 556 1365 1512 360 3643
Fragments arranged by size:
3643 1512 1365 556 360
RsrII CG'GwC_CG
Cuts at: 5631 5631
Size: 7436
SacI G_AGCT'C
Cuts at: 317 4450 317
Size: 4133 3303
SalI G'TCGA_C
Cuts at: 4473 4473
Size: 7436
SapI GCTCTTCn'nnn_
Cuts at: 669 5459 5669 669
Size: 4790 210 2436
Fragments arranged by size:
4790 2436 210
SbfI CC_TGCA'GG
Cuts at: 4483 4483
Size: 7436
ScaI AGT'ACT
Cuts at: 1947 2070 6953 1947
Size: 123 4883 2430
Fragments arranged by size:
4883 2430 123
SfcI C'TryA_G
Cuts at: 33 259 356 905 1521 2720 3168 4194 4479
Size: 226 97 549 616 1199 448 1026 285
Cuts at: 4479 5164 6157 6348 33
Size: 685 993 191 1121
Fragments arranged by size:
1199 1121 1026 993 685 616 549 448
285 226 191 97
SfoI GGC'GCC
Cuts at: 266 4868 5116 266
Size: 4602 248 2586
Fragments arranged by size:
4602 2586 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7436
SgrAI Cr'CCGG_yG
Cuts at: 3371 3371
Size: 7436
SmaI CCC'GGG
Cuts at: 158 4458 158
Size: 4300 3136
SmlI C'TyrA_G
Cuts at: 1612 3452 4591 5506 5998 6260 1612
Size: 1840 1139 915 492 262 2788
Fragments arranged by size:
2788 1840 1139 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7436
SphI G_CATG'C
Cuts at: 4489 4989 4489
Size: 500 6936
SspI AAT'ATT
Cuts at: 376 525 1176 2674 3619 376
Size: 149 651 1498 945 4193
Fragments arranged by size:
4193 1498 945 651 149
StyI C'CwwG_G
Cuts at: 107 4087 4289 4570 4924 107
Size: 3980 202 281 354 2619
Fragments arranged by size:
3980 2619 354 281 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5019 5152 5611 5820 5019
Size: 133 459 209 6635
Fragments arranged by size:
6635 459 209 133
TatI w'GTAC_w
Cuts at: 593 632 741 1945 2062 2068 3822 6951 593
Size: 39 109 1204 117 6 1754 3129 1078
Fragments arranged by size:
3129 1754 1204 1078 117 109 39 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4125 4157 4323 4355 6953 6985 4125
Size: 32 166 32 2598 32 4576
Fragments arranged by size:
4576 2598 166 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5233 5233
Size: 7436
XbaI T'CTAG_A
Cuts at: 918 1950 2364 2452 4467 918
Size: 1032 414 88 2015 3887
Fragments arranged by size:
3887 2015 1032 414 88
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1122 2466 2900 4931 1122
Size: 1344 434 2031 3627
Fragments arranged by size:
3627 2031 1344 434
XmaI C'CCGG_G
Cuts at: 156 4456 156
Size: 4300 3136
XmnI GAAnn'nnTTC
Cuts at: 1934 3837 1934
Size: 1903 5533
ZraI GAC'GTC
Cuts at: 279 279
Size: 7436
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PciI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI ApaI AscI BaeI BbvCI
BcgI BsgI BsiWI BssHII BstEII BstZ17I ClaI DraIII
FseI FspAI HindIII HpaI NdeI NotI NruI PacI
PasI PflMI PfoI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI StuI SwaI
XhoI