Restriction map for
pF1KB7338
(Circular) (Six-base) MAPSORT of: pF1KB7338.seq Check: 4327 from: 1 to: 6302
pF1KB7338 6302 bp
With 182 enzymes: SgfI *
July 22, 2009 17:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 625 2454 625
Size: 1829 4473
AccI GT'mk_AC
Cuts at: 3340 3340
Size: 6302
Acc65I G'GTAC_C
Cuts at: 3318 3318
Size: 6302
AclI AA'CG_TT
Cuts at: 6142 6142
Size: 6302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 624 1203 4127 4559 5306 5574 624
Size: 579 2924 432 747 268 1352
Fragments arranged by size:
2924 1352 747 579 432 268
AflIII A'CryG_T
Cuts at: 2832 3654 4758 5735 2832
Size: 822 1104 977 3399
Fragments arranged by size:
3399 1104 977 822
AgeI A'CCGG_T
Cuts at: 1560 4690 1560
Size: 3130 3172
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 6270
AlwNI CAG_nnn'CTG
Cuts at: 922 1587 3607 5174 922
Size: 665 2020 1567 2050
Fragments arranged by size:
2050 2020 1567 665
ApaI G_GGCC'C
Cuts at: 2973 2973
Size: 6302
ApaLI G'TGCA_C
Cuts at: 5072 5072
Size: 6302
ApoI r'AATT_y
Cuts at: 968 987 1038 1153 1882 2659 2842 3306 5655
Size: 19 51 115 729 777 183 464 2349
Cuts at: 5655 5911 968
Size: 256 1359
Fragments arranged by size:
2349 1359 777 729 464 256 183 115
51 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 6302
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6302
AvaI C'yCGr_G
Cuts at: 115 134 2651 3322 115
Size: 19 2517 671 3095
Fragments arranged by size:
3095 2517 671 19
BamHI G'GATC_C
Cuts at: 3327 3327
Size: 6302
BanI G'GyrC_C
Cuts at: 147 336 613 1131 1243 3318 3732 3980 4015
Size: 189 277 518 112 2075 414 248 35
Cuts at: 4015 147
Size: 2434
Fragments arranged by size:
2434 2075 518 414 277 248 189 112
35
BanII G_rGCy'C
Cuts at: 1210 2540 2777 2860 2973 3316 4346 1210
Size: 1330 237 83 113 343 1030 3166
Fragments arranged by size:
3166 1330 1030 343 237 113 83
BbeI G_GCGC'C
Cuts at: 151 340 3736 3984 151
Size: 189 3396 248 2469
Fragments arranged by size:
3396 2469 248 189
BbsI GAAGACnn'nnnn_
Cuts at: 1800 2042 1800
Size: 242 6060
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 258 292 2361 2395 258
Size: 34 2069 34 4165
Fragments arranged by size:
4165 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 904 1300 4200 4961 6302 904
Size: 396 2900 761 1341 904
Fragments arranged by size:
2900 1341 904 761 396
BclI T'GATC_A
Cuts at: 791 3822 791
Size: 3031 3271
BglI GCCn_nnn'nGGC
Cuts at: 217 266 217
Size: 49 6253
BglII A'GATC_T
Cuts at: 3817 5854 3817
Size: 2037 4265
BlpI GC'TnA_GC
Cuts at: 2776 3414 2776
Size: 638 5664
Bme1580I G_kGCm'C
Cuts at: 1134 2973 3927 4020 5076 1134
Size: 1839 954 93 1056 2360
Fragments arranged by size:
2360 1839 1056 954 93
BmgBI CAC'GTC
Cuts at: 234 2350 234
Size: 2116 4186
BmrI ACTGGGnnnn_n'
Cuts at: 601 947 2529 3514 3595 3930 6074 601
Size: 346 1582 985 81 335 2144 829
Fragments arranged by size:
2144 1582 985 829 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4669 4669
Size: 6302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 594 866 2647 2968 594
Size: 272 1781 321 3928
Fragments arranged by size:
3928 1781 321 272
Bpu10I CC'TnA_GC
Cuts at: 2771 3198 2771
Size: 427 5875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2374 3478 4357 4849 5147 2374
Size: 1104 879 492 298 3529
Fragments arranged by size:
3529 1104 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 737 1217 1283 6006 737
Size: 480 66 4723 1033
Fragments arranged by size:
4723 1033 480 66
BsaAI yAC'GTr
Cuts at: 3655 3655
Size: 6302
BsaBI GATnn'nnATC
Cuts at: 6 1790 2084 2108 3816 5853 6
Size: 1784 294 24 1708 2037 455
Fragments arranged by size:
2037 1784 1708 455 294 24
BsaHI Gr'CG_yC
Cuts at: 148 308 337 3733 3981 4683 6179 148
Size: 160 29 3396 248 702 1496 271
Fragments arranged by size:
3396 1496 702 271 248 160 29
BsaWI w'CCGG_w
Cuts at: 687 1450 1560 3491 4012 4690 4964 5111 5522
Size: 763 110 1931 521 678 274 147 411
Cuts at: 5522 687
Size: 1467
Fragments arranged by size:
1931 1467 763 678 521 411 274 147
110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 509 539 1251 1281 509
Size: 30 712 30 5530
Fragments arranged by size:
5530 712 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 992 1204 1487 2492 2548 2563 2566 2945 3230
Size: 212 283 1005 56 15 3 379 285
Cuts at: 3230 992
Size: 4064
Fragments arranged by size:
4064 1005 379 285 283 212 56 15
3
BseYI C'CCAG_C
Cuts at: 186 925 1580 3727 4485 5062 5558 186
Size: 739 655 2147 758 577 496 930
Fragments arranged by size:
2147 930 758 739 655 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 1576 2572 2930 444
Size: 1132 996 358 3816
Fragments arranged by size:
3816 1132 996 358
BsiEI CG_ry'CG
Cuts at: 104 182 3844 3890 5098 104
Size: 78 3662 46 1208 1308
Fragments arranged by size:
3662 1308 1208 78 46
BsiHKAI G_wGCw'C
Cuts at: 561 984 1210 1367 2777 3316 4094 4284 5076
Size: 423 226 157 1410 539 778 190 792
Cuts at: 5076 561
Size: 1787
Fragments arranged by size:
1787 1410 792 778 539 423 226 190
157
BsmBI CGTCTCn'nnnn_
Cuts at: 1243 1243
Size: 6302
Bsp1286I G_dGCh'C
Cuts at: 561 984 1134 1210 1367 2540 2777 2860 2973
Size: 423 150 76 157 1173 237 83 113
Cuts at: 2973 3316 3927 4020 4094 4284 4346 5076 561
Size: 343 611 93 74 190 62 730 1787
Fragments arranged by size:
1787 1173 730 611 423 343 237 190
157 150 113 93 83 76 74 62
BspEI T'CCGG_A
Cuts at: 687 1450 5522 687
Size: 763 4072 1467
Fragments arranged by size:
4072 1467 763
BspHI T'CATG_A
Cuts at: 5478 5478
Size: 6302
BspMI ACCTGCnnnn'nnnn_
Cuts at: 625 2454 3352 3566 3868 4249 625
Size: 1829 898 214 302 381 2678
Fragments arranged by size:
2678 1829 898 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4594 4648 6132 6297 50
Size: 4544 54 1484 165 55
Fragments arranged by size:
4544 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 684 815 1316 1700 4214 684
Size: 131 501 384 2514 2772
Fragments arranged by size:
2772 2514 501 384 131
BsrFI r'CCGG_y
Cuts at: 1560 4300 4481 4690 1560
Size: 2740 181 209 3172
Fragments arranged by size:
3172 2740 209 181
BsrGI T'GTAC_A
Cuts at: 251 2829 2878 251
Size: 2578 49 3675
Fragments arranged by size:
3675 2578 49
BssSI C'ACGA_G
Cuts at: 4573 4931 4573
Size: 358 5944
BstAPI GCAn_nnn'nTGC
Cuts at: 3679 3679
Size: 6302
BstBI TT'CG_AA
Cuts at: 4663 4663
Size: 6302
BstXI CCAn_nnnn'nTGG
Cuts at: 2277 2277
Size: 6302
BstYI r'GATC_y
Cuts at: 1 1232 3327 3794 3817 4152 4398 5410 5496
Size: 1231 2095 467 23 335 246 1012 86
Cuts at: 5496 5508 5854 1
Size: 12 346 449
Fragments arranged by size:
2095 1231 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 844 3242 3290 844
Size: 2398 48 3856
Fragments arranged by size:
3856 2398 48
BtgI C'CryG_G
Cuts at: 126 176 287 404 126
Size: 50 111 117 6024
Fragments arranged by size:
6024 117 111 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 4252 4433 4596 1786
Size: 2466 181 163 3492
Fragments arranged by size:
3492 2466 181 163
BtsI GCAGTG_nn'
Cuts at: 427 794 2450 2710 2918 3545 427
Size: 367 1656 260 208 627 3184
Fragments arranged by size:
3184 1656 627 367 260 208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1408 1443 1408
Size: 35 6267
DraI TTT'AAA
Cuts at: 1947 3301 1947
Size: 1354 4948
DrdI GACnn_nn'nnGTC
Cuts at: 4008 4866 4008
Size: 858 5444
EaeI y'GGCC_r
Cuts at: 179 3887 4061 4452 4479 5824 179
Size: 3708 174 391 27 1345 657
Fragments arranged by size:
3708 1345 657 391 174 27
EagI C'GGCC_G
Cuts at: 179 3887 179
Size: 3708 2594
EarI CTCTTCn'nnn_
Cuts at: 1222 1783 2183 2525 2815 4325 4535 1222
Size: 561 400 342 290 1510 210 2989
Fragments arranged by size:
2989 1510 561 400 342 290 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4510 4820 4966 6112 4510
Size: 310 146 1146 4700
Fragments arranged by size:
4700 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1208 2775 3314 1208
Size: 1567 539 4196
Fragments arranged by size:
4196 1567 539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 594 624 866 1203 2647 2968 4127 4559 5306
Size: 30 242 337 1444 321 1159 432 747
Cuts at: 5306 5574 594
Size: 268 1322
Fragments arranged by size:
1444 1322 1159 747 432 337 321 268
242 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1337 1337
Size: 6302
EcoO109I rG'GnC_Cy
Cuts at: 603 760 2695 3441 603
Size: 157 1935 746 3464
Fragments arranged by size:
3464 1935 746 157
EcoRI G'AATT_C
Cuts at: 968 1882 2842 3306 968
Size: 914 960 464 3964
Fragments arranged by size:
3964 960 914 464
EcoRV GAT'ATC
Cuts at: 366 813 840 366
Size: 447 27 5828
Fragments arranged by size:
5828 447 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4321 4321
Size: 6302
FspI TGC'GCA
Cuts at: 419 4083 419
Size: 3664 2638
HaeII r_GCGC'y
Cuts at: 151 340 647 2783 3736 3984 5006 5985 151
Size: 189 307 2136 953 248 1022 979 468
Fragments arranged by size:
2136 1022 979 953 468 307 248 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 776 808 1593 1625 1882 1914 4144
Size: 32 260 32 785 32 257 32 2230
Cuts at: 4144 4174 4176 4206 484
Size: 30 2 30 2580
Fragments arranged by size:
2580 2230 785 260 257 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1098 3341 1098
Size: 2243 4059
HpaI GTT'AAC
Cuts at: 1098 1098
Size: 6302
KasI G'GCGC_C
Cuts at: 147 336 3732 3980 147
Size: 189 3396 248 2469
Fragments arranged by size:
3396 2469 248 189
KpnI G_GTAC'C
Cuts at: 3322 3322
Size: 6302
MluI A'CGCG_T
Cuts at: 5735 5735
Size: 6302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2184 2426 2538 4973 5157 2184
Size: 242 112 2435 184 3329
Fragments arranged by size:
3329 2435 242 184 112
MscI TGG'CCA
Cuts at: 4063 4063
Size: 6302
MslI CAynn'nnrTG
Cuts at: 1327 2020 2579 2964 4291 4418 1327
Size: 693 559 385 1327 127 3211
Fragments arranged by size:
3211 1327 693 559 385 127
MspA1I CmG'CkG
Cuts at: 128 153 178 257 406 3270 3413 3727 4087
Size: 25 25 79 149 2864 143 314 360
Cuts at: 4087 5100 5345 128
Size: 1013 245 1085
Fragments arranged by size:
2864 1085 1013 360 314 245 149 143
79 25 25
NaeI GCC'GGC
Cuts at: 4483 4483
Size: 6302
NarI GG'CG_CC
Cuts at: 148 337 3733 3981 148
Size: 189 3396 248 2469
Fragments arranged by size:
3396 2469 248 189
NdeI CA'TA_TG
Cuts at: 2157 2157
Size: 6302
NgoMIV G'CCGG_C
Cuts at: 4481 4481
Size: 6302
NotI GC'GGCC_GC
Cuts at: 179 179
Size: 6302
NspI r_CATG'y
Cuts at: 1441 2836 3355 3855 4762 1441
Size: 1395 519 500 907 2981
Fragments arranged by size:
2981 1395 907 519 500
PasI CC'CwG_GG
Cuts at: 2325 2325
Size: 6302
PciI A'CATG_T
Cuts at: 2832 4758 2832
Size: 1926 4376
PflMI CCAn_nnn'nTGG
Cuts at: 509 509
Size: 6302
PfoI T'CCnGG_A
Cuts at: 2624 2624
Size: 6302
PmeI GTTT'AAAC
Cuts at: 3301 3301
Size: 6302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5441 5473 5441
Size: 32 6270
PpuMI rG'GwC_Cy
Cuts at: 603 760 2695 603
Size: 157 1935 4210
Fragments arranged by size:
4210 1935 157
PsiI TTA'TAA
Cuts at: 734 1373 734
Size: 639 5663
PspOMI G'GGCC_C
Cuts at: 2969 2969
Size: 6302
PstI C_TGCA'G
Cuts at: 2705 3349 4034 2705
Size: 644 685 4973
Fragments arranged by size:
4973 685 644
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6302
PvuII CAG'CTG
Cuts at: 3270 3727 4087 3270
Size: 457 360 5485
Fragments arranged by size:
5485 457 360
RsrII CG'GwC_CG
Cuts at: 4497 4497
Size: 6302
SacI G_AGCT'C
Cuts at: 1210 2777 3316 1210
Size: 1567 539 4196
Fragments arranged by size:
4196 1567 539
SacII CC_GC'GG
Cuts at: 129 179 407 129
Size: 50 228 6024
Fragments arranged by size:
6024 228 50
SalI G'TCGA_C
Cuts at: 3339 3339
Size: 6302
SapI GCTCTTCn'nnn_
Cuts at: 1783 4325 4535 1783
Size: 2542 210 3550
Fragments arranged by size:
3550 2542 210
SbfI CC_TGCA'GG
Cuts at: 3349 3349
Size: 6302
ScaI AGT'ACT
Cuts at: 5819 5819
Size: 6302
SfcI C'TryA_G
Cuts at: 33 1571 1983 2456 2701 3345 4030 5023 5214
Size: 1538 412 473 245 644 685 993 191
Cuts at: 5214 33
Size: 1121
Fragments arranged by size:
1538 1121 993 685 644 473 412 245
191
SfoI GGC'GCC
Cuts at: 149 338 3734 3982 149
Size: 189 3396 248 2469
Fragments arranged by size:
3396 2469 248 189
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6302
SmaI CCC'GGG
Cuts at: 117 136 2653 3324 117
Size: 19 2517 671 3095
Fragments arranged by size:
3095 2517 671 19
SmlI C'TyrA_G
Cuts at: 2353 3457 4372 4864 5126 2353
Size: 1104 915 492 262 3529
Fragments arranged by size:
3529 1104 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6302
SphI G_CATG'C
Cuts at: 3355 3855 3355
Size: 500 5802
SspI AAT'ATT
Cuts at: 1973 2595 1973
Size: 622 5680
StuI AGG'CCT
Cuts at: 1955 1955
Size: 6302
StyI C'CwwG_G
Cuts at: 263 397 906 2289 3436 3790 263
Size: 134 509 1383 1147 354 2775
Fragments arranged by size:
2775 1383 1147 509 354 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1507 1610 3271 3885 4018 4477 4686 1507
Size: 103 1661 614 133 459 209 3123
Fragments arranged by size:
3123 1661 614 459 209 133 103
TatI w'GTAC_w
Cuts at: 251 2249 2829 2878 5817 251
Size: 1998 580 49 2939 736
Fragments arranged by size:
2939 1998 736 580 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 509 533 541 565 1427 1459 2552 2584 5819
Size: 24 8 24 862 32 1093 32 3235
Cuts at: 5819 5851 509
Size: 32 960
Fragments arranged by size:
3235 1093 960 862 32 32 32 24
24 8
Tth111I GACn'n_nGTC
Cuts at: 4099 4099
Size: 6302
XbaI T'CTAG_A
Cuts at: 3333 3333
Size: 6302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1237 2628 3797 1237
Size: 1391 1169 3742
Fragments arranged by size:
3742 1391 1169
XmaI C'CCGG_G
Cuts at: 115 134 2651 3322 115
Size: 19 2517 671 3095
Fragments arranged by size:
3095 2517 671 19
XmnI GAAnn'nnTTC
Cuts at: 2663 2663
Size: 6302
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmI BssHII BstEII
BstZ17I ClaI DraIII FseI FspAI HindIII MfeI NcoI
NheI NruI NsiI PacI PmlI PshAI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI