Restriction map for
pF1KB7341
(Circular) (Six-base) MAPSORT of: pF1KB7341.seq Check: 2526 from: 1 to: 8645
pF1KB7341 8645 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 995 3600 3647 4454 5375 5683 995
Size: 2605 47 807 921 308 3957
Fragments arranged by size:
3957 2605 921 807 308 47
Acc65I G'GTAC_C
Cuts at: 5661 5661
Size: 8645
AclI AA'CG_TT
Cuts at: 8485 8485
Size: 8645
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 887 1292 1540 1871 2165 2291 2401 2948 3275
Size: 405 248 331 294 126 110 547 327
Cuts at: 3275 3412 3998 4544 6470 6902 7649 7917 887
Size: 137 586 546 1926 432 747 268 1615
Fragments arranged by size:
1926 1615 747 586 547 546 432 405
331 327 294 268 248 137 126 110
AflII C'TTAA_G
Cuts at: 2289 4501 2289
Size: 2212 6433
AflIII A'CryG_T
Cuts at: 2721 4449 5072 5997 7101 8078 2721
Size: 1728 623 925 1104 977 3288
Fragments arranged by size:
3288 1728 1104 977 925 623
AgeI A'CCGG_T
Cuts at: 7033 7033
Size: 8645
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4498 4530 4498
Size: 32 8613
AlwNI CAG_nnn'CTG
Cuts at: 379 1075 3390 4478 4708 5950 7517 379
Size: 696 2315 1088 230 1242 1567 1507
Fragments arranged by size:
2315 1567 1507 1242 1088 696 230
ApaLI G'TGCA_C
Cuts at: 7415 7415
Size: 8645
ApoI r'AATT_y
Cuts at: 666 786 913 1043 1130 1452 2080 2791 2851
Size: 120 127 130 87 322 628 711 60
Cuts at: 2851 2865 2920 2977 3120 3605 3900 4227 4421
Size: 14 55 57 143 485 295 327 194
Cuts at: 4421 4579 4600 5046 5301 5585 5649 7998 8254
Size: 158 21 446 255 284 64 2349 256
Cuts at: 8254 666
Size: 1057
Fragments arranged by size:
2349 1057 711 628 485 446 327 322
295 284 256 255 194 158 143 130
127 120 87 64 60 57 55 21
14
AseI AT'TA_AT
Cuts at: 20 2038 20
Size: 2018 6627
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8645
AvaI C'yCGr_G
Cuts at: 3426 5665 3426
Size: 2239 6406
BamHI G'GATC_C
Cuts at: 5670 5670
Size: 8645
BanI G'GyrC_C
Cuts at: 5661 6075 6323 6358 5661
Size: 414 248 35 7948
Fragments arranged by size:
7948 414 248 35
BanII G_rGCy'C
Cuts at: 2068 5659 6689 2068
Size: 3591 1030 4024
Fragments arranged by size:
4024 3591 1030
BbeI G_GCGC'C
Cuts at: 6079 6327 6079
Size: 248 8397
BbsI GAAGACnn'nnnn_
Cuts at: 256 337 542 1364 3178 3263 4532 256
Size: 81 205 822 1814 85 1269 4369
Fragments arranged by size:
4369 1814 1269 822 205 85 81
BciVI GTATCCnnnnn_n'
Cuts at: 1471 4306 6543 7304 8645 1471
Size: 2835 2237 761 1341 1471
Fragments arranged by size:
2835 2237 1471 1341 761
BclI T'GATC_A
Cuts at: 4866 6165 4866
Size: 1299 7346
BglI GCCn_nnn'nGGC
Cuts at: 130 130
Size: 8645
BglII A'GATC_T
Cuts at: 750 1894 2131 2820 6160 8197 750
Size: 1144 237 689 3340 2037 1198
Fragments arranged by size:
3340 2037 1198 1144 689 237
BlpI GC'TnA_GC
Cuts at: 5757 5757
Size: 8645
Bme1580I G_kGCm'C
Cuts at: 3546 6270 6363 7419 3546
Size: 2724 93 1056 4772
Fragments arranged by size:
4772 2724 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 870 1580 5857 5938 6273 8417 870
Size: 710 4277 81 335 2144 1098
Fragments arranged by size:
4277 2144 1098 710 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5471 7012 5471
Size: 1541 7104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3088 3187 5243 3088
Size: 99 2056 6490
Fragments arranged by size:
6490 2056 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2971 4660 5209 5821 6700 7192 7490 2971
Size: 1689 549 612 879 492 298 4126
Fragments arranged by size:
4126 1689 879 612 549 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4480 8349 4480
Size: 3869 4776
BsaAI yAC'GTr
Cuts at: 5998 5998
Size: 8645
BsaBI GATnn'nnATC
Cuts at: 6 4835 6159 8196 6
Size: 4829 1324 2037 455
Fragments arranged by size:
4829 2037 1324 455
BsaHI Gr'CG_yC
Cuts at: 6076 6324 7026 8522 6076
Size: 248 702 1496 6199
Fragments arranged by size:
6199 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4924 5834 6355 7033 7307 7454 7865 4924
Size: 910 521 678 274 147 411 5704
Fragments arranged by size:
5704 910 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4319 4349 4319
Size: 30 8615
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2289 4036 2289
Size: 1747 6898
BseYI C'CCAG_C
Cuts at: 1049 3587 3985 4051 6070 6828 7405 7901 1049
Size: 2538 398 66 2019 758 577 496 1793
Fragments arranged by size:
2538 2019 1793 758 577 496 398 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4742 5554 4742
Size: 812 7833
BsiEI CG_ry'CG
Cuts at: 104 6187 6233 7441 104
Size: 6083 46 1208 1308
Fragments arranged by size:
6083 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3029 3801 5659 6437 6627 7419 3029
Size: 772 1858 778 190 792 4255
Fragments arranged by size:
4255 1858 792 778 772 190
BsmI GAATG_Cn'
Cuts at: 3582 3875 4068 3582
Size: 293 193 8159
Fragments arranged by size:
8159 293 193
BsmBI CGTCTCn'nnnn_
Cuts at: 4922 4922
Size: 8645
Bsp1286I G_dGCh'C
Cuts at: 2068 3029 3546 3801 5659 6270 6363 6437 6627
Size: 961 517 255 1858 611 93 74 190
Cuts at: 6627 6689 7419 2068
Size: 62 730 3294
Fragments arranged by size:
3294 1858 961 730 611 517 255 190
93 74 62
BspEI T'CCGG_A
Cuts at: 7865 7865
Size: 8645
BspHI T'CATG_A
Cuts at: 2903 7821 2903
Size: 4918 3727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 220 1044 5507 5695 5909 6211 6592 220
Size: 824 4463 188 214 302 381 2273
Fragments arranged by size:
4463 2273 824 381 302 214 188
BsrBI CCG'CTC
Cuts at: 50 6937 6991 8475 8640 50
Size: 6887 54 1484 165 55
Fragments arranged by size:
6887 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 6557 6557
Size: 8645
BsrFI r'CCGG_y
Cuts at: 6643 6824 7033 6643
Size: 181 209 8255
Fragments arranged by size:
8255 209 181
BsrGI T'GTAC_A
Cuts at: 1956 2684 4383 1956
Size: 728 1699 6218
Fragments arranged by size:
6218 1699 728
BssSI C'ACGA_G
Cuts at: 6916 7274 6916
Size: 358 8287
BstAPI GCAn_nnn'nTGC
Cuts at: 1345 3535 6022 1345
Size: 2190 2487 3968
Fragments arranged by size:
3968 2487 2190
BstBI TT'CG_AA
Cuts at: 5511 7006 5511
Size: 1495 7150
BstXI CCAn_nnnn'nTGG
Cuts at: 3553 3595 3816 3553
Size: 42 221 8382
Fragments arranged by size:
8382 221 42
BstYI r'GATC_y
Cuts at: 1 750 1894 2131 2820 5325 5670 6137 6160
Size: 749 1144 237 689 2505 345 467 23
Cuts at: 6160 6495 6741 7753 7839 7851 8197 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2505 1144 1012 749 689 467 449 346
345 335 246 237 86 23 12
BstZ17I GTA'TAC
Cuts at: 5376 5376
Size: 8645
BtgI C'CryG_G
Cuts at: 107 125 107
Size: 18 8627
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6595 6776 6939 6595
Size: 181 163 8301
Fragments arranged by size:
8301 181 163
BtsI GCAGTG_nn'
Cuts at: 2699 3536 3600 5888 2699
Size: 837 64 2288 5456
Fragments arranged by size:
5456 2288 837 64
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3494 3529 3494
Size: 35 8610
DraI TTT'AAA
Cuts at: 548 920 2387 2796 3444 3831 4544 5045 5644
Size: 372 1467 409 648 387 713 501 599
Cuts at: 5644 548
Size: 3549
Fragments arranged by size:
3549 1467 713 648 599 501 409 387
372
DrdI GACnn_nn'nnGTC
Cuts at: 1003 6351 7209 1003
Size: 5348 858 2439
Fragments arranged by size:
5348 2439 858
EaeI y'GGCC_r
Cuts at: 122 6230 6404 6795 6822 8167 122
Size: 6108 174 391 27 1345 600
Fragments arranged by size:
6108 1345 600 391 174 27
EagI C'GGCC_G
Cuts at: 6230 6230
Size: 8645
EarI CTCTTCn'nnn_
Cuts at: 941 1416 3219 3357 3461 3588 5316 5410 5462
Size: 475 1803 138 104 127 1728 94 52
Cuts at: 5462 6668 6878 941
Size: 1206 210 2708
Fragments arranged by size:
2708 1803 1728 1206 475 210 138 127
104 94 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 134 1091 6853 7163 7309 8455 134
Size: 957 5762 310 146 1146 324
Fragments arranged by size:
5762 1146 957 324 310 146
EcoICRI GAG'CTC
Cuts at: 5657 5657
Size: 8645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 887 1292 1540 1871 2165 2291 2401 2948 3088
Size: 405 248 331 294 126 110 547 140
Cuts at: 3088 3187 3275 3412 3998 4544 5243 6470 6902
Size: 99 88 137 586 546 699 1227 432
Cuts at: 6902 7649 7917 887
Size: 747 268 1615
Fragments arranged by size:
1615 1227 747 699 586 547 546 432
405 331 294 268 248 140 137 126
110 99 88
EcoNI CCTnn'n_nnAGG
Cuts at: 689 1756 689
Size: 1067 7578
EcoO109I rG'GnC_Cy
Cuts at: 117 5784 117
Size: 5667 2978
EcoRI G'AATT_C
Cuts at: 2865 4600 5301 5585 5649 2865
Size: 1735 701 284 64 5861
Fragments arranged by size:
5861 1735 701 284 64
EcoRV GAT'ATC
Cuts at: 4284 5423 4284
Size: 1139 7506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 590 1718 2037 2210 2631 3280 6664 590
Size: 1128 319 173 421 649 3384 2571
Fragments arranged by size:
3384 2571 1128 649 421 319 173
FspI TGC'GCA
Cuts at: 6426 6426
Size: 8645
HaeII r_GCGC'y
Cuts at: 2298 6079 6327 7349 8328 2298
Size: 3781 248 1022 979 2615
Fragments arranged by size:
3781 2615 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 4088 4120 5059 5091 5444 5476 6487
Size: 32 3866 32 939 32 353 32 1011
Cuts at: 6487 6517 6519 6549 190
Size: 30 2 30 2286
Fragments arranged by size:
3866 2286 1011 939 353 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 384 640 996 1886 1972 4265 5684 384
Size: 256 356 890 86 2293 1419 3345
Fragments arranged by size:
3345 2293 1419 890 356 256 86
HindIII A'AGCT_T
Cuts at: 762 1825 2240 762
Size: 1063 415 7167
Fragments arranged by size:
7167 1063 415
HpaI GTT'AAC
Cuts at: 4265 4265
Size: 8645
KasI G'GCGC_C
Cuts at: 6075 6323 6075
Size: 248 8397
KpnI G_GTAC'C
Cuts at: 5665 5665
Size: 8645
MfeI C'AATT_G
Cuts at: 506 2221 506
Size: 1715 6930
MluI A'CGCG_T
Cuts at: 8078 8078
Size: 8645
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3951 5122 5353 7316 7500 3951
Size: 1171 231 1963 184 5096
Fragments arranged by size:
5096 1963 1171 231 184
MscI TGG'CCA
Cuts at: 124 6406 124
Size: 6282 2363
MslI CAynn'nnrTG
Cuts at: 6634 6761 6634
Size: 127 8518
MspA1I CmG'CkG
Cuts at: 139 2527 5239 5629 5756 6070 6430 7443 7688
Size: 2388 2712 390 127 314 360 1013 245
Cuts at: 7688 139
Size: 1096
Fragments arranged by size:
2712 2388 1096 1013 390 360 314 245
127
NaeI GCC'GGC
Cuts at: 6826 6826
Size: 8645
NarI GG'CG_CC
Cuts at: 6076 6324 6076
Size: 248 8397
NcoI C'CATG_G
Cuts at: 107 125 107
Size: 18 8627
NdeI CA'TA_TG
Cuts at: 906 906
Size: 8645
NgoMIV G'CCGG_C
Cuts at: 6824 6824
Size: 8645
NspI r_CATG'y
Cuts at: 2725 3264 5076 5698 6198 7105 2725
Size: 539 1812 622 500 907 4265
Fragments arranged by size:
4265 1812 907 622 539 500
PciI A'CATG_T
Cuts at: 2721 5072 7101 2721
Size: 2351 2029 4265
Fragments arranged by size:
4265 2351 2029
PmeI GTTT'AAAC
Cuts at: 5644 5644
Size: 8645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7784 7816 7784
Size: 32 8613
PpuMI rG'GwC_Cy
Cuts at: 117 117
Size: 8645
PsiI TTA'TAA
Cuts at: 2359 3496 2359
Size: 1137 7508
PstI C_TGCA'G
Cuts at: 138 3531 5010 5244 5692 6377 138
Size: 3393 1479 234 448 685 2406
Fragments arranged by size:
3393 2406 1479 685 448 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8645
PvuII CAG'CTG
Cuts at: 139 2527 5239 5629 6070 6430 139
Size: 2388 2712 390 441 360 2354
Fragments arranged by size:
2712 2388 2354 441 390 360
RsrII CG'GwC_CG
Cuts at: 6840 6840
Size: 8645
SacI G_AGCT'C
Cuts at: 5659 5659
Size: 8645
SalI G'TCGA_C
Cuts at: 994 5682 994
Size: 4688 3957
SapI GCTCTTCn'nnn_
Cuts at: 3357 6668 6878 3357
Size: 3311 210 5124
Fragments arranged by size:
5124 3311 210
SbfI CC_TGCA'GG
Cuts at: 5692 5692
Size: 8645
ScaI AGT'ACT
Cuts at: 573 2097 3035 4173 8162 573
Size: 1524 938 1138 3989 1056
Fragments arranged by size:
3989 1524 1138 1056 938
SfcI C'TryA_G
Cuts at: 33 134 540 1922 3527 5006 5240 5688 6373
Size: 101 406 1382 1605 1479 234 448 685
Cuts at: 6373 7366 7557 33
Size: 993 191 1121
Fragments arranged by size:
1605 1479 1382 1121 993 685 448 406
234 191 101
SfoI GGC'GCC
Cuts at: 6077 6325 6077
Size: 248 8397
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8645
SmaI CCC'GGG
Cuts at: 5667 5667
Size: 8645
SmlI C'TyrA_G
Cuts at: 2289 2950 4501 4639 5188 5800 6715 7207 7469
Size: 661 1551 138 549 612 915 492 262
Cuts at: 7469 2289
Size: 3465
Fragments arranged by size:
3465 1551 915 661 612 549 492 262
138
SpeI A'CTAG_T
Cuts at: 67 4389 67
Size: 4322 4323
SphI G_CATG'C
Cuts at: 5698 6198 5698
Size: 500 8145
SspI AAT'ATT
Cuts at: 232 291 483 2995 4823 232
Size: 59 192 2512 1828 4054
Fragments arranged by size:
4054 2512 1828 192 59
StyI C'CwwG_G
Cuts at: 107 125 770 3815 5779 6133 107
Size: 18 645 3045 1964 354 2619
Fragments arranged by size:
3045 2619 1964 645 354 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 333 2732 6228 6361 6820 7029 333
Size: 2399 3496 133 459 209 1949
Fragments arranged by size:
3496 2399 1949 459 209 133
TatI w'GTAC_w
Cuts at: 571 1956 2095 2684 3033 4171 4383 8160 571
Size: 1385 139 589 349 1138 212 3777 1056
Fragments arranged by size:
3777 1385 1138 1056 589 349 212 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2728 2760 2947 2979 4308 4340 8162 8194 2728
Size: 32 187 32 1329 32 3822 32 3179
Fragments arranged by size:
3822 3179 1329 187 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6442 6442
Size: 8645
XbaI T'CTAG_A
Cuts at: 850 1296 2302 5676 850
Size: 446 1006 3374 3819
Fragments arranged by size:
3819 3374 1006 446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6140 6140
Size: 8645
XmaI C'CCGG_G
Cuts at: 5665 5665
Size: 8645
XmnI GAAnn'nnTTC
Cuts at: 312 620 5585 312
Size: 308 4965 3372
Fragments arranged by size:
4965 3372 308
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BssHII BstEII Bsu36I ClaI DraIII FseI FspAI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI