Restriction map for
pF1KB7345
(Circular) (Six-base) MAPSORT of: pF1KB7345.seq Check: 3601 from: 1 to: 9782
pF1KB7345 9782 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4557 4557
Size: 9782
AccI GT'mk_AC
Cuts at: 1379 6820 1379
Size: 5441 4341
Acc65I G'GTAC_C
Cuts at: 4471 6798 4471
Size: 2327 7455
AclI AA'CG_TT
Cuts at: 2595 9622 2595
Size: 7027 2755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 845 910 1259 1343 1394 1499 1649 1948
Size: 560 65 349 84 51 105 150 299
Cuts at: 1948 2165 2473 3566 4322 4820 5645 6128 7607
Size: 217 308 1093 756 498 825 483 1479
Cuts at: 7607 8039 8786 9054 285
Size: 432 747 268 1013
Fragments arranged by size:
1479 1093 1013 825 756 747 560 498
483 432 349 308 299 268 217 150
105 84 65 51
AfeI AGC'GCT
Cuts at: 2962 2962
Size: 9782
AflII C'TTAA_G
Cuts at: 2964 5464 6370 2964
Size: 2500 906 6376
Fragments arranged by size:
6376 2500 906
AflIII A'CryG_T
Cuts at: 5019 6774 7134 8238 9215 5019
Size: 1755 360 1104 977 5586
Fragments arranged by size:
5586 1755 1104 977 360
AgeI A'CCGG_T
Cuts at: 8170 8170
Size: 9782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1669 6182 1669
Size: 4513 5269
AlwNI CAG_nnn'CTG
Cuts at: 1306 1993 2251 3739 4381 4634 7087 8654 1306
Size: 687 258 1488 642 253 2453 1567 2434
Fragments arranged by size:
2453 2434 1567 1488 687 642 258 253
ApaLI G'TGCA_C
Cuts at: 8552 8552
Size: 9782
ApoI r'AATT_y
Cuts at: 496 517 1423 1728 1783 2136 2804 6090 6137
Size: 21 906 305 55 353 668 3286 47
Cuts at: 6137 6786 9135 9391 496
Size: 649 2349 256 887
Fragments arranged by size:
3286 2349 906 887 668 649 353 305
256 55 47 21
AseI AT'TA_AT
Cuts at: 20 338 1223 6305 20
Size: 318 885 5082 3497
Fragments arranged by size:
5082 3497 885 318
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9782
AvaI C'yCGr_G
Cuts at: 4406 5364 6802 4406
Size: 958 1438 7386
Fragments arranged by size:
7386 1438 958
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3413 3446 5219 5252 3413
Size: 33 1773 33 7943
Fragments arranged by size:
7943 1773 33 33
BamHI G'GATC_C
Cuts at: 3549 4006 4860 6807 3549
Size: 457 854 1947 6524
Fragments arranged by size:
6524 1947 854 457
BanI G'GyrC_C
Cuts at: 111 760 3534 4279 4318 4471 5161 5368 6798
Size: 649 2774 745 39 153 690 207 1430
Cuts at: 6798 7212 7460 7495 111
Size: 414 248 35 2398
Fragments arranged by size:
2774 2398 1430 745 690 649 414 248
207 153 39 35
BanII G_rGCy'C
Cuts at: 3806 5386 6131 6796 7826 3806
Size: 1580 745 665 1030 5762
Fragments arranged by size:
5762 1580 1030 745 665
BbeI G_GCGC'C
Cuts at: 7216 7464 7216
Size: 248 9534
BbsI GAAGACnn'nnnn_
Cuts at: 569 722 4418 6022 6741 569
Size: 153 3696 1604 719 3610
Fragments arranged by size:
3696 3610 1604 719 153
BbvCI CC'TCA_GC
Cuts at: 287 800 287
Size: 513 9269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2466 2500 2466
Size: 34 9748
BciVI GTATCCnnnnn_n'
Cuts at: 3163 3233 7680 8441 9782 3163
Size: 70 4447 761 1341 3163
Fragments arranged by size:
4447 3163 1341 761 70
BclI T'GATC_A
Cuts at: 4527 7302 4527
Size: 2775 7007
BglII A'GATC_T
Cuts at: 936 1485 7297 9334 936
Size: 549 5812 2037 1384
Fragments arranged by size:
5812 2037 1384 549
BlpI GC'TnA_GC
Cuts at: 821 2294 2753 6894 821
Size: 1473 459 4141 3709
Fragments arranged by size:
4141 3709 1473 459
Bme1580I G_kGCm'C
Cuts at: 765 1132 1439 1658 5364 7407 7500 8556 765
Size: 367 307 219 3706 2043 93 1056 1991
Fragments arranged by size:
3706 2043 1991 1056 367 307 219 93
BmrI ACTGGGnnnn_n'
Cuts at: 690 1493 3791 5381 6141 6994 7075 7410 9554
Size: 803 2298 1590 760 853 81 335 2144
Cuts at: 9554 690
Size: 918
Fragments arranged by size:
2298 2144 1590 918 853 803 760 335
81
BmtI G_CTAG'C
Cuts at: 2801 2801
Size: 9782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8149 8149
Size: 9782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 493 2335 2472 4220 5279 313
Size: 180 1842 137 1748 1059 4816
Fragments arranged by size:
4816 1842 1748 1059 180 137
Bpu10I CC'TnA_GC
Cuts at: 287 800 287
Size: 513 9269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1896 2060 2902 5362 5554 6000 6391 6958
Size: 815 164 842 2460 192 446 391 567
Cuts at: 6958 7837 8329 8627 1081
Size: 879 492 298 2236
Fragments arranged by size:
2460 2236 879 842 815 567 492 446
391 298 192 164
BsaI GGTCTCn'nnnn_
Cuts at: 9486 9486
Size: 9782
BsaAI yAC'GTr
Cuts at: 7135 7135
Size: 9782
BsaBI GATnn'nnATC
Cuts at: 6 3548 5972 7296 9333 6
Size: 3542 2424 1324 2037 455
Fragments arranged by size:
3542 2424 2037 1324 455
BsaHI Gr'CG_yC
Cuts at: 7213 7461 8163 9659 7213
Size: 248 702 1496 7336
Fragments arranged by size:
7336 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5249 6971 7492 8170 8444 8591 9002 5249
Size: 1722 521 678 274 147 411 6029
Fragments arranged by size:
6029 1722 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 122 152 1747 1777 4242 4272 6087 6117 6257
Size: 30 1595 30 2465 30 1815 30 140
Cuts at: 6257 6287 122
Size: 30 3617
Fragments arranged by size:
3617 2465 1815 1595 140 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 725 2971 4223 5554 5557 725
Size: 2246 1252 1331 3 4950
Fragments arranged by size:
4950 2246 1331 1252 3
BseYI C'CCAG_C
Cuts at: 6457 7207 7965 8542 9038 6457
Size: 750 758 577 496 7201
Fragments arranged by size:
7201 758 750 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 5167 164
Size: 5003 4779
BsiEI CG_ry'CG
Cuts at: 104 7324 7370 8578 104
Size: 7220 46 1208 1308
Fragments arranged by size:
7220 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 6796 7574 7764 8556 6796
Size: 778 190 792 8022
Fragments arranged by size:
8022 792 778 190
BsmI GAATG_Cn'
Cuts at: 830 2217 830
Size: 1387 8395
Bsp1286I G_dGCh'C
Cuts at: 765 1132 1439 1658 3806 5364 5386 6131 6796
Size: 367 307 219 2148 1558 22 745 665
Cuts at: 6796 7407 7500 7574 7764 7826 8556 765
Size: 611 93 74 190 62 730 1991
Fragments arranged by size:
2148 1991 1558 745 730 665 611 367
307 219 190 93 74 62 22
BspEI T'CCGG_A
Cuts at: 9002 9002
Size: 9782
BspHI T'CATG_A
Cuts at: 1611 1683 1842 2060 2285 2370 4823 8958 1611
Size: 72 159 218 225 85 2453 4135 2435
Fragments arranged by size:
4135 2453 2435 225 218 159 85 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1299 4557 4805 5445 6422 6832 7046 7348 7729
Size: 3258 248 640 977 410 214 302 381
Cuts at: 7729 1299
Size: 3352
Fragments arranged by size:
3352 3258 977 640 410 381 302 248
214
BsrBI CCG'CTC
Cuts at: 50 8074 8128 9612 9777 50
Size: 8024 54 1484 165 55
Fragments arranged by size:
8024 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 212 2618 5559 7694 212
Size: 2406 2941 2135 2300
Fragments arranged by size:
2941 2406 2300 2135
BsrFI r'CCGG_y
Cuts at: 7780 7961 8170 7780
Size: 181 209 9392
Fragments arranged by size:
9392 209 181
BssSI C'ACGA_G
Cuts at: 8053 8411 8053
Size: 358 9424
BstAPI GCAn_nnn'nTGC
Cuts at: 1455 1758 4033 6298 7159 1455
Size: 303 2275 2265 861 4078
Fragments arranged by size:
4078 2275 2265 861 303
BstBI TT'CG_AA
Cuts at: 8143 8143
Size: 9782
BstYI r'GATC_y
Cuts at: 1 361 936 1485 2184 3104 3466 3549 4006
Size: 360 575 549 699 920 362 83 457
Cuts at: 4006 4377 4860 6807 7274 7297 7632 7878 8890
Size: 371 483 1947 467 23 335 246 1012
Cuts at: 8890 8976 8988 9334 1
Size: 86 12 346 449
Fragments arranged by size:
1947 1012 920 699 575 549 483 467
457 449 371 362 360 346 335 246
86 83 23 12
Bsu36I CC'TnA_GG
Cuts at: 665 3341 4865 665
Size: 2676 1524 5582
Fragments arranged by size:
5582 2676 1524
BtgI C'CryG_G
Cuts at: 107 269 1810 2011 5206 107
Size: 162 1541 201 3195 4683
Fragments arranged by size:
4683 3195 1541 201 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7732 7913 8076 7732
Size: 181 163 9438
Fragments arranged by size:
9438 181 163
BtsI GCAGTG_nn'
Cuts at: 1658 6068 7025 1658
Size: 4410 957 4415
Fragments arranged by size:
4415 4410 957
ClaI AT'CG_AT
Cuts at: 2943 2943
Size: 9782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 6528 6563 6528
Size: 35 9747
DraI TTT'AAA
Cuts at: 2472 3332 4943 5844 6438 6781 2472
Size: 860 1611 901 594 343 5473
Fragments arranged by size:
5473 1611 901 860 594 343
DraIII CAC_nnn'GTG
Cuts at: 1560 1560
Size: 9782
DrdI GACnn_nn'nnGTC
Cuts at: 3877 4420 7488 8346 3877
Size: 543 3068 858 5313
Fragments arranged by size:
5313 3068 858 543
EaeI y'GGCC_r
Cuts at: 7367 7541 7932 7959 9304 7367
Size: 174 391 27 1345 7845
Fragments arranged by size:
7845 1345 391 174 27
EagI C'GGCC_G
Cuts at: 7367 7367
Size: 9782
EarI CTCTTCn'nnn_
Cuts at: 304 2927 2948 3116 3356 3380 3701 7805 8015
Size: 2623 21 168 240 24 321 4104 210
Cuts at: 8015 304
Size: 2071
Fragments arranged by size:
4104 2623 2071 321 240 210 168 24
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7990 8300 8446 9592 7990
Size: 310 146 1146 8180
Fragments arranged by size:
8180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 6794 6794
Size: 9782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 313 493 845 910 1259 1343 1394 1499
Size: 28 180 352 65 349 84 51 105
Cuts at: 1499 1649 1948 2165 2335 2472 2473 3566 4220
Size: 150 299 217 170 137 1 1093 654
Cuts at: 4220 4322 4820 5279 5645 6128 7607 8039 8786
Size: 102 498 459 366 483 1479 432 747
Cuts at: 8786 9054 285
Size: 268 1013
Fragments arranged by size:
1479 1093 1013 747 654 498 483 459
432 366 352 349 299 268 217 180
170 150 137 105 102 84 65 51
28 1
EcoNI CCTnn'n_nnAGG
Cuts at: 641 641
Size: 9782
EcoO109I rG'GnC_Cy
Cuts at: 282 1663 2493 4546 5299 6921 282
Size: 1381 830 2053 753 1622 3143
Fragments arranged by size:
3143 2053 1622 1381 830 753
EcoRI G'AATT_C
Cuts at: 1423 6786 1423
Size: 5363 4419
EcoRV GAT'ATC
Cuts at: 2858 2858
Size: 9782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 265 940 2503 3694 7801 265
Size: 675 1563 1191 4107 2246
Fragments arranged by size:
4107 2246 1563 1191 675
FspI TGC'GCA
Cuts at: 7563 7563
Size: 9782
HaeII r_GCGC'y
Cuts at: 2964 7216 7464 8486 9465 2964
Size: 4252 248 1022 979 3281
Fragments arranged by size:
4252 3281 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1747 1779 2847 2879 3034 3066 3609 3641 4032
Size: 32 1068 32 155 32 543 32 391
Cuts at: 4032 4064 4443 4475 4957 4989 7624 7654 7656
Size: 32 379 32 482 32 2635 30 2
Cuts at: 7656 7686 1747
Size: 30 3843
Fragments arranged by size:
3843 2635 1068 543 482 391 379 155
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 428 903 3812 6821 428
Size: 475 2909 3009 3389
Fragments arranged by size:
3389 3009 2909 475
HindIII A'AGCT_T
Cuts at: 3449 3449
Size: 9782
HpaI GTT'AAC
Cuts at: 428 903 428
Size: 475 9307
KasI G'GCGC_C
Cuts at: 7212 7460 7212
Size: 248 9534
KpnI G_GTAC'C
Cuts at: 4475 6802 4475
Size: 2327 7455
MfeI C'AATT_G
Cuts at: 5231 5231
Size: 9782
MluI A'CGCG_T
Cuts at: 9215 9215
Size: 9782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 339 2619 3619 3980 6166 6243 6396 6528 8453
Size: 2280 1000 361 2186 77 153 132 1925
Cuts at: 8453 8637 339
Size: 184 1484
Fragments arranged by size:
2280 2186 1925 1484 1000 361 184 153
132 77
MscI TGG'CCA
Cuts at: 7543 7543
Size: 9782
MslI CAynn'nnrTG
Cuts at: 1432 2065 2633 3332 4214 4466 5205 6386 7771
Size: 633 568 699 882 252 739 1181 1385
Cuts at: 7771 7898 1432
Size: 127 3316
Fragments arranged by size:
3316 1385 1181 882 739 699 633 568
252 127
MspA1I CmG'CkG
Cuts at: 1081 2265 4744 4803 5950 6502 6544 6893 7207
Size: 1184 2479 59 1147 552 42 349 314
Cuts at: 7207 7567 8580 8825 1081
Size: 360 1013 245 2038
Fragments arranged by size:
2479 2038 1184 1147 1013 552 360 349
314 245 59 42
NaeI GCC'GGC
Cuts at: 7963 7963
Size: 9782
NarI GG'CG_CC
Cuts at: 7213 7461 7213
Size: 248 9534
NcoI C'CATG_G
Cuts at: 107 1810 5206 107
Size: 1703 3396 4683
Fragments arranged by size:
4683 3396 1703
NgoMIV G'CCGG_C
Cuts at: 7961 7961
Size: 9782
NheI G'CTAG_C
Cuts at: 2797 2797
Size: 9782
NspI r_CATG'y
Cuts at: 1215 5023 6778 6835 7335 8242 1215
Size: 3808 1755 57 500 907 2755
Fragments arranged by size:
3808 2755 1755 907 500 57
PasI CC'CwG_GG
Cuts at: 6687 6687
Size: 9782
PciI A'CATG_T
Cuts at: 5019 6774 8238 5019
Size: 1755 1464 6563
Fragments arranged by size:
6563 1755 1464
PflMI CCAn_nnn'nTGG
Cuts at: 757 1306 757
Size: 549 9233
PfoI T'CCnGG_A
Cuts at: 1172 1305 5295 1172
Size: 133 3990 5659
Fragments arranged by size:
5659 3990 133
PmeI GTTT'AAAC
Cuts at: 6781 6781
Size: 9782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 155 187 6268 6300 6604 6636 8921 8953 155
Size: 32 6081 32 304 32 2285 32 984
Fragments arranged by size:
6081 2285 984 304 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 282 2493 4546 5299 282
Size: 2211 2053 753 4765
Fragments arranged by size:
4765 2211 2053 753
PshAI GACnn'nnGTC
Cuts at: 1360 1360
Size: 9782
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3728 3760 3728
Size: 32 9750
PstI C_TGCA'G
Cuts at: 884 5456 5510 6054 6161 6433 6829 7514 884
Size: 4572 54 544 107 272 396 685 3152
Fragments arranged by size:
4572 3152 685 544 396 272 107 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9782
PvuII CAG'CTG
Cuts at: 1081 2265 4744 4803 5950 6502 6544 7207 7567
Size: 1184 2479 59 1147 552 42 663 360
Cuts at: 7567 1081
Size: 3296
Fragments arranged by size:
3296 2479 1184 1147 663 552 360 59
42
RsrII CG'GwC_CG
Cuts at: 7977 7977
Size: 9782
SacI G_AGCT'C
Cuts at: 6796 6796
Size: 9782
SalI G'TCGA_C
Cuts at: 6819 6819
Size: 9782
SapI GCTCTTCn'nnn_
Cuts at: 2927 7805 8015 2927
Size: 4878 210 4694
Fragments arranged by size:
4878 4694 210
SbfI CC_TGCA'GG
Cuts at: 6829 6829
Size: 9782
ScaI AGT'ACT
Cuts at: 1000 2483 9299 1000
Size: 1483 6816 1483
Fragments arranged by size:
6816 1483 1483
SexAI A'CCwGG_T
Cuts at: 4282 4691 4282
Size: 409 9373
SfcI C'TryA_G
Cuts at: 33 392 880 1714 3833 5395 5452 5506 6050
Size: 359 488 834 2119 1562 57 54 544
Cuts at: 6050 6157 6429 6825 7510 8503 8694 33
Size: 107 272 396 685 993 191 1121
Fragments arranged by size:
2119 1562 1121 993 834 685 544 488
396 359 272 191 107 57 54
SfoI GGC'GCC
Cuts at: 7214 7462 7214
Size: 248 9534
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9782
SmaI CCC'GGG
Cuts at: 5366 6804 5366
Size: 1438 8344
SmlI C'TyrA_G
Cuts at: 1060 1875 2075 2917 2964 4406 5377 5464 5569
Size: 815 200 842 47 1442 971 87 105
Cuts at: 5569 6015 6370 6406 6937 7852 8344 8606 1060
Size: 446 355 36 531 915 492 262 2236
Fragments arranged by size:
2236 1442 971 915 842 815 531 492
446 355 262 200 105 87 47 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9782
SphI G_CATG'C
Cuts at: 1215 6835 7335 1215
Size: 5620 500 3662
Fragments arranged by size:
5620 3662 500
SspI AAT'ATT
Cuts at: 1149 2611 3569 1149
Size: 1462 958 7362
Fragments arranged by size:
7362 1462 958
StuI AGG'CCT
Cuts at: 208 5330 208
Size: 5122 4660
StyI C'CwwG_G
Cuts at: 107 608 1810 5206 6563 6916 7270 107
Size: 501 1202 3396 1357 353 354 2619
Fragments arranged by size:
3396 2619 1357 1202 501 354 353
SwaI ATTT'AAAT
Cuts at: 3332 4943 3332
Size: 1611 8171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 709 1512 2560 3710 6471 7365 7498 7957
Size: 580 803 1048 1150 2761 894 133 459
Cuts at: 7957 8166 129
Size: 209 1745
Fragments arranged by size:
2761 1745 1150 1048 894 803 580 459
209 133
TatI w'GTAC_w
Cuts at: 998 1064 2481 4748 9297 998
Size: 66 1417 2267 4549 1483
Fragments arranged by size:
4549 2267 1483 1417 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 154 1546 1578 1965 1997 5722 5754 6257
Size: 32 1392 32 387 32 3725 32 503
Cuts at: 6257 6289 6458 6490 9299 9331 122
Size: 32 169 32 2809 32 573
Fragments arranged by size:
3725 2809 1392 573 503 387 169 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7579 7579
Size: 9782
XbaI T'CTAG_A
Cuts at: 939 5976 6813 939
Size: 5037 837 3908
Fragments arranged by size:
5037 3908 837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 219 2391 2635 2754 7277 219
Size: 2172 244 119 4523 2724
Fragments arranged by size:
4523 2724 2172 244 119
XhoI C'TCGA_G
Cuts at: 4406 4406
Size: 9782
XmaI C'CCGG_G
Cuts at: 5364 6802 5364
Size: 1438 8344
XmnI GAAnn'nnTTC
Cuts at: 920 1787 2891 5069 5270 6064 920
Size: 867 1104 2178 201 794 4638
Fragments arranged by size:
4638 2178 1104 867 794 201
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AhdI AleI AloI ApaI AscI AvrII BfrBI
BglI BmgBI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I FseI FspAI NdeI NotI NruI NsiI PacI
PmlI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI
SnaBI SrfI ZraI