Restriction map for
pF1KB7347
(Circular) (Six-base) MAPSORT of: pF1KB7347.seq Check: 1818 from: 1 to: 6422
pF1KB7347 6422 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1201 1201
Size: 6422
AatII G_ACGT'C
Cuts at: 2294 2294
Size: 6422
AccI GT'mk_AC
Cuts at: 2870 3460 2870
Size: 590 5832
Acc65I G'GTAC_C
Cuts at: 1399 3438 1399
Size: 2039 4383
AclI AA'CG_TT
Cuts at: 6262 6262
Size: 6422
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1079 1813 1972 2128 3262 4247 4679 5426 5694
Size: 734 159 156 1134 985 432 747 268
Cuts at: 5694 1079
Size: 1807
Fragments arranged by size:
1807 1134 985 747 734 432 268 159
156
AfeI AGC'GCT
Cuts at: 404 877 404
Size: 473 5949
AflIII A'CryG_T
Cuts at: 2960 3774 4878 5855 2960
Size: 814 1104 977 3527
Fragments arranged by size:
3527 1104 977 814
AgeI A'CCGG_T
Cuts at: 4810 4810
Size: 6422
AleI CACnn'nnGTG
Cuts at: 829 829
Size: 6422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3058 3370 3058
Size: 312 6110
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2014 2046 2014
Size: 32 6390
AlwNI CAG_nnn'CTG
Cuts at: 684 2676 3727 5294 684
Size: 1992 1051 1567 1812
Fragments arranged by size:
1992 1812 1567 1051
ApaI G_GGCC'C
Cuts at: 204 289 729 818 1020 204
Size: 85 440 89 202 5606
Fragments arranged by size:
5606 440 202 89 85
ApaLI G'TGCA_C
Cuts at: 5192 5192
Size: 6422
ApoI r'AATT_y
Cuts at: 1798 3426 5775 6031 1798
Size: 1628 2349 256 2189
Fragments arranged by size:
2349 2189 1628 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6422
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6422
AvaI C'yCGr_G
Cuts at: 148 197 314 412 728 785 1376 1910 2365
Size: 49 117 98 316 57 591 534 455
Cuts at: 2365 2725 2910 3048 3204 3370 3442 148
Size: 360 185 138 156 166 72 3128
Fragments arranged by size:
3128 591 534 455 360 316 185 166
156 138 117 98 72 57 49
AvrII C'CTAG_G
Cuts at: 1287 1287
Size: 6422
BamHI G'GATC_C
Cuts at: 1368 3447 1368
Size: 2079 4343
BanI G'GyrC_C
Cuts at: 549 583 1150 1399 1571 1660 1702 2433 3253
Size: 34 567 249 172 89 42 731 820
Cuts at: 3253 3438 3852 4100 4135 549
Size: 185 414 248 35 2836
Fragments arranged by size:
2836 820 731 567 414 249 248 185
172 89 42 35 34
BanII G_rGCy'C
Cuts at: 155 177 204 289 321 436 729 767 786
Size: 22 27 85 32 115 293 38 19
Cuts at: 786 818 1020 1092 1307 1384 1868 1984 2360
Size: 32 202 72 215 77 484 116 376
Cuts at: 2360 2395 2726 2801 3436 4466 155
Size: 35 331 75 635 1030 2111
Fragments arranged by size:
2111 1030 635 484 376 331 293 215
202 116 115 85 77 75 72 38
35 32 32 27 22 19
BbeI G_GCGC'C
Cuts at: 3257 3856 4104 3257
Size: 599 248 5575
Fragments arranged by size:
5575 599 248
BbsI GAAGACnn'nnnn_
Cuts at: 921 1161 2551 2656 2710 921
Size: 240 1390 105 54 4633
Fragments arranged by size:
4633 1390 240 105 54
BbvCI CC'TCA_GC
Cuts at: 2693 2693
Size: 6422
BciVI GTATCCnnnnn_n'
Cuts at: 2458 4320 5081 6422 2458
Size: 1862 761 1341 2458
Fragments arranged by size:
2458 1862 1341 761
BclI T'GATC_A
Cuts at: 833 3942 833
Size: 3109 3313
BglI GCCn_nnn'nGGC
Cuts at: 343 380 790 1555 343
Size: 37 410 765 5210
Fragments arranged by size:
5210 765 410 37
BglII A'GATC_T
Cuts at: 3937 5974 3937
Size: 2037 4385
BlpI GC'TnA_GC
Cuts at: 3534 3534
Size: 6422
Bme1580I G_kGCm'C
Cuts at: 204 289 554 586 729 818 1020 1663 1707
Size: 85 265 32 143 89 202 643 44
Cuts at: 1707 2340 2992 4047 4140 5196 204
Size: 633 652 1055 93 1056 1430
Fragments arranged by size:
1430 1056 1055 652 643 633 265 202
143 93 89 85 44 32
BmrI ACTGGGnnnn_n'
Cuts at: 3634 3715 4050 6194 3634
Size: 81 335 2144 3862
Fragments arranged by size:
3862 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1128 2414 2604 4789 1128
Size: 1286 190 2185 2761
Fragments arranged by size:
2761 2185 1286 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 907 1510 1843 2284 2374 2599 2620 2653
Size: 107 603 333 441 90 225 21 33
Cuts at: 2653 3409 800
Size: 756 3813
Fragments arranged by size:
3813 756 603 441 333 225 107 90
33 21
Bpu10I CC'TnA_GC
Cuts at: 2248 2693 2248
Size: 445 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1076 2131 3192 3598 4477 4969 5267 1076
Size: 1055 1061 406 879 492 298 2231
Fragments arranged by size:
2231 1061 1055 879 492 406 298
BsaI GGTCTCn'nnnn_
Cuts at: 1140 1334 1602 1965 6126 1140
Size: 194 268 363 4161 1436
Fragments arranged by size:
4161 1436 363 268 194
BsaAI yAC'GTr
Cuts at: 3165 3775 3165
Size: 610 5812
BsaBI GATnn'nnATC
Cuts at: 6 3936 5973 6
Size: 3930 2037 455
Fragments arranged by size:
3930 2037 455
BsaHI Gr'CG_yC
Cuts at: 427 2291 2894 3254 3853 4101 4803 6299 427
Size: 1864 603 360 599 248 702 1496 550
Fragments arranged by size:
1864 1496 702 603 599 550 360 248
BsaWI w'CCGG_w
Cuts at: 141 486 1228 2199 3611 4132 4810 5084 5231
Size: 345 742 971 1412 521 678 274 147
Cuts at: 5231 5642 141
Size: 411 921
Fragments arranged by size:
1412 971 921 742 678 521 411 345
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1223 1253 1769 1799 2713 2743 1223
Size: 30 516 30 914 30 4902
Fragments arranged by size:
4902 914 516 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 883 913 1307 1315 1987 2641 883
Size: 30 394 8 672 654 4664
Fragments arranged by size:
4664 672 654 394 30 8
BseYI C'CCAG_C
Cuts at: 438 647 858 903 1004 1543 1735 1830 2256
Size: 209 211 45 101 539 192 95 426
Cuts at: 2256 2468 2921 3847 4605 5182 5678 438
Size: 212 453 926 758 577 496 1182
Fragments arranged by size:
1182 926 758 577 539 496 453 426
212 211 209 192 101 95 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1956 2062 2398 3028 1956
Size: 106 336 630 5350
Fragments arranged by size:
5350 630 336 106
BsiEI CG_ry'CG
Cuts at: 104 3403 3964 4010 5218 104
Size: 3299 561 46 1208 1308
Fragments arranged by size:
3299 1308 1208 561 46
BsiHKAI G_wGCw'C
Cuts at: 1034 1868 1970 2360 3178 3198 3436 4214 4404
Size: 834 102 390 818 20 238 778 190
Cuts at: 4404 5196 1034
Size: 792 2260
Fragments arranged by size:
2260 834 818 792 778 390 238 190
102 20
BsmI GAATG_Cn'
Cuts at: 640 640
Size: 6422
BsmBI CGTCTCn'nnnn_
Cuts at: 1289 1289
Size: 6422
Bsp1286I G_dGCh'C
Cuts at: 155 177 204 289 321 436 554 586 729
Size: 22 27 85 32 115 118 32 143
Cuts at: 729 767 786 818 1020 1034 1092 1307 1384
Size: 38 19 32 202 14 58 215 77
Cuts at: 1384 1663 1707 1868 1970 1984 2340 2360 2395
Size: 279 44 161 102 14 356 20 35
Cuts at: 2395 2726 2801 2992 3178 3198 3436 4047 4140
Size: 331 75 191 186 20 238 611 93
Cuts at: 4140 4214 4404 4466 5196 155
Size: 74 190 62 730 1381
Fragments arranged by size:
1381 730 611 356 331 279 238 215
202 191 190 186 161 143 118 115
102 93 85 77 75 74 62 58
44 38 35 32 32 32 27 22
20 20 19 14 14
BspEI T'CCGG_A
Cuts at: 141 1228 5642 141
Size: 1087 4414 921
Fragments arranged by size:
4414 1087 921
BspHI T'CATG_A
Cuts at: 5598 5598
Size: 6422
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1122 1201 1254 2825 3329 3472 3686 3988 4369
Size: 79 53 1571 504 143 214 302 381
Cuts at: 4369 1122
Size: 3175
Fragments arranged by size:
3175 1571 504 381 302 214 143 79
53
BsrBI CCG'CTC
Cuts at: 50 130 219 2215 3375 4714 4768 6252 6417
Size: 80 89 1996 1160 1339 54 1484 165
Cuts at: 6417 50
Size: 55
Fragments arranged by size:
1996 1484 1339 1160 165 89 80 55
54
BsrDI GCAATG_nn'
Cuts at: 4334 4334
Size: 6422
BsrFI r'CCGG_y
Cuts at: 506 1343 1396 1574 2284 2657 4420 4601 4810
Size: 837 53 178 710 373 1763 181 209
Cuts at: 4810 506
Size: 2118
Fragments arranged by size:
2118 1763 837 710 373 209 181 178
53
BssHII G'CGCG_C
Cuts at: 652 652
Size: 6422
BssSI C'ACGA_G
Cuts at: 478 1449 4693 5051 478
Size: 971 3244 358 1849
Fragments arranged by size:
3244 1849 971 358
BstAPI GCAn_nnn'nTGC
Cuts at: 852 2385 3799 852
Size: 1533 1414 3475
Fragments arranged by size:
3475 1533 1414
BstBI TT'CG_AA
Cuts at: 4783 4783
Size: 6422
BstEII G'GTnAC_C
Cuts at: 666 666
Size: 6422
BstXI CCAn_nnnn'nTGG
Cuts at: 1654 2929 1654
Size: 1275 5147
BstYI r'GATC_y
Cuts at: 1 1368 2604 3362 3447 3914 3937 4272 4518
Size: 1367 1236 758 85 467 23 335 246
Cuts at: 4518 5530 5616 5628 5974 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1367 1236 1012 758 467 449 346 335
246 86 85 23 12
Bsu36I CC'TnA_GG
Cuts at: 796 3014 796
Size: 2218 4204
BtgI C'CryG_G
Cuts at: 587 611 713 758 1625 2160 2166 2423 2498
Size: 24 102 45 867 535 6 257 75
Cuts at: 2498 2984 3088 3342 587
Size: 486 104 254 3667
Fragments arranged by size:
3667 867 535 486 257 254 104 102
75 45 24 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4372 4553 4716 4372
Size: 181 163 6078
Fragments arranged by size:
6078 181 163
BtsI GCAGTG_nn'
Cuts at: 836 1968 2340 2470 3665 836
Size: 1132 372 130 1195 3593
Fragments arranged by size:
3593 1195 1132 372 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1169 1204 1169
Size: 35 6387
DraI TTT'AAA
Cuts at: 3421 3421
Size: 6422
DraIII CAC_nnn'GTG
Cuts at: 852 852
Size: 6422
DrdI GACnn_nn'nnGTC
Cuts at: 4128 4986 4128
Size: 858 5564
EaeI y'GGCC_r
Cuts at: 332 344 508 983 2163 2282 2303 3400 4007
Size: 12 164 475 1180 119 21 1097 607
Cuts at: 4007 4181 4572 4599 5944 332
Size: 174 391 27 1345 810
Fragments arranged by size:
1345 1180 1097 810 607 475 391 174
164 119 27 21 12
EagI C'GGCC_G
Cuts at: 3400 4007 3400
Size: 607 5815
EarI CTCTTCn'nnn_
Cuts at: 1991 4445 4655 1991
Size: 2454 210 3758
Fragments arranged by size:
3758 2454 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 249 592 1383 1816 4630 4940 5086 6232 249
Size: 343 791 433 2814 310 146 1146 439
Fragments arranged by size:
2814 1146 791 439 433 343 310 146
EcoICRI GAG'CTC
Cuts at: 1866 2358 3434 1866
Size: 492 1076 4854
Fragments arranged by size:
4854 1076 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 907 1079 1510 1813 1843 1972 2128 2284
Size: 107 172 431 303 30 129 156 156
Cuts at: 2284 2374 2599 2620 2653 3262 3409 4247 4679
Size: 90 225 21 33 609 147 838 432
Cuts at: 4679 5426 5694 800
Size: 747 268 1528
Fragments arranged by size:
1528 838 747 609 432 431 303 268
225 172 156 156 147 129 107 90
33 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 799 886 1939 2059 799
Size: 87 1053 120 5162
Fragments arranged by size:
5162 1053 120 87
EcoO109I rG'GnC_Cy
Cuts at: 201 279 286 681 725 792 1016 1266 1284
Size: 78 7 395 44 67 224 250 18
Cuts at: 1284 1547 1924 1992 2253 3561 201
Size: 263 377 68 261 1308 3062
Fragments arranged by size:
3062 1308 395 377 263 261 250 224
78 68 67 44 18 7
EcoRI G'AATT_C
Cuts at: 3426 3426
Size: 6422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1863 4441 1863
Size: 2578 3844
FspI TGC'GCA
Cuts at: 1084 1262 3028 4203 1084
Size: 178 1766 1175 3303
Fragments arranged by size:
3303 1766 1175 178
FspAI rTGC'GCAy
Cuts at: 3028 3028
Size: 6422
HaeII r_GCGC'y
Cuts at: 406 879 1101 2271 3257 3856 4104 5126 6105
Size: 473 222 1170 986 599 248 1022 979
Cuts at: 6105 406
Size: 723
Fragments arranged by size:
1170 1022 986 979 723 599 473 248
222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2129 2161 2664 2696 4264 4294 4296 4326 2129
Size: 32 503 32 1568 30 2 30 4225
Fragments arranged by size:
4225 1568 503 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3117 3461 3117
Size: 344 6078
KasI G'GCGC_C
Cuts at: 3253 3852 4100 3253
Size: 599 248 5575
Fragments arranged by size:
5575 599 248
KpnI G_GTAC'C
Cuts at: 1403 3442 1403
Size: 2039 4383
MluI A'CGCG_T
Cuts at: 5855 5855
Size: 6422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5093 5277 5093
Size: 184 6238
MscI TGG'CCA
Cuts at: 2305 4183 2305
Size: 1878 4544
MslI CAynn'nnrTG
Cuts at: 829 841 1360 1591 1652 2311 3163 4411 4538
Size: 12 519 231 61 659 852 1248 127
Cuts at: 4538 829
Size: 2713
Fragments arranged by size:
2713 1248 852 659 519 231 127 61
12
MspA1I CmG'CkG
Cuts at: 613 1237 1730 2226 2925 3090 3533 3847 4207
Size: 624 493 496 699 165 443 314 360
Cuts at: 4207 5220 5465 613
Size: 1013 245 1570
Fragments arranged by size:
1570 1013 699 624 496 493 443 360
314 245 165
NaeI GCC'GGC
Cuts at: 508 2286 4603 508
Size: 1778 2317 2327
Fragments arranged by size:
2327 2317 1778
NarI GG'CG_CC
Cuts at: 3254 3853 4101 3254
Size: 599 248 5575
Fragments arranged by size:
5575 599 248
NcoI C'CATG_G
Cuts at: 758 2423 2498 758
Size: 1665 75 4682
Fragments arranged by size:
4682 1665 75
NdeI CA'TA_TG
Cuts at: 3008 3008
Size: 6422
NgoMIV G'CCGG_C
Cuts at: 506 2284 4601 506
Size: 1778 2317 2327
Fragments arranged by size:
2327 2317 1778
NspI r_CATG'y
Cuts at: 1365 1590 1716 2964 3003 3475 3975 4882 1365
Size: 225 126 1248 39 472 500 907 2905
Fragments arranged by size:
2905 1248 907 500 472 225 126 39
PasI CC'CwG_GG
Cuts at: 290 554 818 1680 1804 290
Size: 264 264 862 124 4908
Fragments arranged by size:
4908 862 264 264 124
PciI A'CATG_T
Cuts at: 2960 4878 2960
Size: 1918 4504
PflMI CCAn_nnn'nTGG
Cuts at: 1147 1746 1147
Size: 599 5823
PfoI T'CCnGG_A
Cuts at: 884 1253 1859 2612 884
Size: 369 606 753 4694
Fragments arranged by size:
4694 753 606 369
PmeI GTTT'AAAC
Cuts at: 3421 3421
Size: 6422
PmlI CAC'GTG
Cuts at: 3165 3165
Size: 6422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5561 5593 5561
Size: 32 6390
PpuMI rG'GwC_Cy
Cuts at: 279 681 1266 1284 1992 2253 279
Size: 402 585 18 708 261 4448
Fragments arranged by size:
4448 708 585 402 261 18
PspOMI G'GGCC_C
Cuts at: 200 285 725 814 1016 200
Size: 85 440 89 202 5606
Fragments arranged by size:
5606 440 202 89 85
PstI C_TGCA'G
Cuts at: 245 1130 3359 3469 4154 245
Size: 885 2229 110 685 2513
Fragments arranged by size:
2513 2229 885 685 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6422
PvuII CAG'CTG
Cuts at: 1237 2226 3847 4207 1237
Size: 989 1621 360 3452
Fragments arranged by size:
3452 1621 989 360
RsrII CG'GwC_CG
Cuts at: 4617 4617
Size: 6422
SacI G_AGCT'C
Cuts at: 1868 2360 3436 1868
Size: 492 1076 4854
Fragments arranged by size:
4854 1076 492
SacII CC_GC'GG
Cuts at: 614 3091 614
Size: 2477 3945
SalI G'TCGA_C
Cuts at: 3459 3459
Size: 6422
SanDI GG'GwC_CC
Cuts at: 2253 2253
Size: 6422
SapI GCTCTTCn'nnn_
Cuts at: 4445 4655 4445
Size: 210 6212
SbfI CC_TGCA'GG
Cuts at: 3359 3469 3359
Size: 110 6312
ScaI AGT'ACT
Cuts at: 2587 2731 5939 2587
Size: 144 3208 3070
Fragments arranged by size:
3208 3070 144
SexAI A'CCwGG_T
Cuts at: 3212 3212
Size: 6422
SfcI C'TryA_G
Cuts at: 33 241 1126 3355 3465 4150 5143 5334 33
Size: 208 885 2229 110 685 993 191 1121
Fragments arranged by size:
2229 1121 993 885 685 208 191 110
SfiI GGCCn_nnn'nGGCC
Cuts at: 1555 1555
Size: 6422
SfoI GGC'GCC
Cuts at: 3255 3854 4102 3255
Size: 599 248 5575
Fragments arranged by size:
5575 599 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6422
SmaI CCC'GGG
Cuts at: 150 199 730 787 1378 3444 150
Size: 49 531 57 591 2066 3128
Fragments arranged by size:
3128 2066 591 531 57 49
SmlI C'TyrA_G
Cuts at: 1091 2146 3171 3577 4492 4984 5246 1091
Size: 1055 1025 406 915 492 262 2267
Fragments arranged by size:
2267 1055 1025 915 492 406 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6422
SphI G_CATG'C
Cuts at: 3003 3475 3975 3003
Size: 472 500 5450
Fragments arranged by size:
5450 500 472
StuI AGG'CCT
Cuts at: 979 2698 3041 979
Size: 1719 343 4360
Fragments arranged by size:
4360 1719 343
StyI C'CwwG_G
Cuts at: 758 767 980 1287 2414 2423 2498 3556 3910
Size: 9 213 307 1127 9 75 1058 354
Cuts at: 3910 758
Size: 3270
Fragments arranged by size:
3270 1127 1058 354 307 213 75 9
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 678 850 928 1212 1664 1690 4005 4138 4597
Size: 172 78 284 452 26 2315 133 459
Cuts at: 4597 4806 678
Size: 209 2294
Fragments arranged by size:
2315 2294 459 452 284 209 172 133
78 26
TatI w'GTAC_w
Cuts at: 2585 2729 2750 5937 2585
Size: 144 21 3187 3070
Fragments arranged by size:
3187 3070 144 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1223 1255 2212 2244 2957 2989 3177 3209 5939
Size: 32 957 32 713 32 188 32 2730
Cuts at: 5939 5971 1223
Size: 32 1674
Fragments arranged by size:
2730 1674 957 713 188 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1165 3112 4219 1165
Size: 1947 1107 3368
Fragments arranged by size:
3368 1947 1107
XbaI T'CTAG_A
Cuts at: 3453 3453
Size: 6422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 765 1362 1743 2808 2964 3917 765
Size: 597 381 1065 156 953 3270
Fragments arranged by size:
3270 1065 953 597 381 156
XmaI C'CCGG_G
Cuts at: 148 197 728 785 1376 3442 148
Size: 49 531 57 591 2066 3128
Fragments arranged by size:
3128 2066 591 531 57 49
XmnI GAAnn'nnTTC
Cuts at: 1798 1798
Size: 6422
ZraI GAC'GTC
Cuts at: 2292 2292
Size: 6422
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI BaeI BcgI BfrBI BmgBI BmtI
BsiWI BsrGI BstZ17I ClaI EcoRV FseI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SgrAI SnaBI SrfI SspI SwaI XhoI