Restriction map for
pF1KB7348
(Circular) (Six-base) MAPSORT of: pF1KB7348.seq Check: 3125 from: 1 to: 6470
pF1KB7348 6470 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3033 3033
Size: 6470
AccI GT'mk_AC
Cuts at: 789 1211 1371 2725 3508 789
Size: 422 160 1354 783 3751
Fragments arranged by size:
3751 1354 783 422 160
Acc65I G'GTAC_C
Cuts at: 3486 3486
Size: 6470
AclI AA'CG_TT
Cuts at: 6310 6310
Size: 6470
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 2784 2817 3280 4295 4727 5474 5742 1148
Size: 1636 33 463 1015 432 747 268 1876
Fragments arranged by size:
1876 1636 1015 747 463 432 268 33
AflII C'TTAA_G
Cuts at: 214 1933 214
Size: 1719 4751
AflIII A'CryG_T
Cuts at: 3822 4926 5903 3822
Size: 1104 977 4389
Fragments arranged by size:
4389 1104 977
AgeI A'CCGG_T
Cuts at: 4858 4858
Size: 6470
AleI CACnn'nnGTG
Cuts at: 610 3118 610
Size: 2508 3962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 332 332
Size: 6470
AlwNI CAG_nnn'CTG
Cuts at: 3775 5342 3775
Size: 1567 4903
ApaI G_GGCC'C
Cuts at: 1384 1384
Size: 6470
ApaLI G'TGCA_C
Cuts at: 624 893 924 1725 5240 624
Size: 269 31 801 3515 1854
Fragments arranged by size:
3515 1854 801 269 31
ApoI r'AATT_y
Cuts at: 1182 1291 2110 2271 2403 2480 2640 2678 2784
Size: 109 819 161 132 77 160 38 106
Cuts at: 2784 2967 3474 5823 6079 1182
Size: 183 507 2349 256 1573
Fragments arranged by size:
2349 1573 819 507 256 183 161 160
132 109 106 77 38
AseI AT'TA_AT
Cuts at: 20 1112 20
Size: 1092 5378
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6470
AvaI C'yCGr_G
Cuts at: 3490 3490
Size: 6470
BamHI G'GATC_C
Cuts at: 2793 3495 2793
Size: 702 5768
BanI G'GyrC_C
Cuts at: 134 1201 3486 3900 4148 4183 134
Size: 1067 2285 414 248 35 2421
Fragments arranged by size:
2421 2285 1067 414 248 35
BanII G_rGCy'C
Cuts at: 158 1384 2262 3299 3484 4514 158
Size: 1226 878 1037 185 1030 2114
Fragments arranged by size:
2114 1226 1037 1030 878 185
BbeI G_GCGC'C
Cuts at: 138 3904 4152 138
Size: 3766 248 2456
Fragments arranged by size:
3766 2456 248
BbsI GAAGACnn'nnnn_
Cuts at: 1340 1751 2054 1340
Size: 411 303 5756
Fragments arranged by size:
5756 411 303
BbvCI CC'TCA_GC
Cuts at: 3002 3002
Size: 6470
BciVI GTATCCnnnnn_n'
Cuts at: 3134 4368 5129 6470 3134
Size: 1234 761 1341 3134
Fragments arranged by size:
3134 1341 1234 761
BclI T'GATC_A
Cuts at: 614 869 1065 2376 3308 3990 614
Size: 255 196 1311 932 682 3094
Fragments arranged by size:
3094 1311 932 682 255 196
BfrBI ATG'CAT
Cuts at: 746 1366 2006 3019 746
Size: 620 640 1013 4197
Fragments arranged by size:
4197 1013 640 620
BglII A'GATC_T
Cuts at: 3139 3985 6022 3139
Size: 846 2037 3587
Fragments arranged by size:
3587 2037 846
BlpI GC'TnA_GC
Cuts at: 674 3582 674
Size: 2908 3562
Bme1580I G_kGCm'C
Cuts at: 628 897 928 1206 1384 1729 4095 4188 5244
Size: 269 31 278 178 345 2366 93 1056
Cuts at: 5244 628
Size: 1854
Fragments arranged by size:
2366 1854 1056 345 278 269 178 93
31
BmrI ACTGGGnnnn_n'
Cuts at: 901 3194 3682 3763 4098 6242 901
Size: 2293 488 81 335 2144 1129
Fragments arranged by size:
2293 2144 1129 488 335 81
BmtI G_CTAG'C
Cuts at: 1081 1081
Size: 6470
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3348 4837 3348
Size: 1489 4981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 1940 161
Size: 1779 4691
Bpu10I CC'TnA_GC
Cuts at: 3002 3002
Size: 6470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 3478 3646 4525 5017 5315 580
Size: 2898 168 879 492 298 1735
Fragments arranged by size:
2898 1735 879 492 298 168
BsaI GGTCTCn'nnnn_
Cuts at: 1030 2299 6174 1030
Size: 1269 3875 1326
Fragments arranged by size:
3875 1326 1269
BsaAI yAC'GTr
Cuts at: 3823 3823
Size: 6470
BsaBI GATnn'nnATC
Cuts at: 6 3325 3984 6021 6
Size: 3319 659 2037 455
Fragments arranged by size:
3319 2037 659 455
BsaHI Gr'CG_yC
Cuts at: 135 3901 4149 4851 6347 135
Size: 3766 248 702 1496 258
Fragments arranged by size:
3766 1496 702 258 248
BsaWI w'CCGG_w
Cuts at: 3659 4180 4858 5132 5279 5690 3659
Size: 521 678 274 147 411 4439
Fragments arranged by size:
4439 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 482 497 1343 1550 482
Size: 15 846 207 5402
Fragments arranged by size:
5402 846 207 15
BseYI C'CCAG_C
Cuts at: 3895 4653 5230 5726 3895
Size: 758 577 496 4639
Fragments arranged by size:
4639 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1742 544
Size: 1198 5272
BsiEI CG_ry'CG
Cuts at: 104 4012 4058 5266 104
Size: 3908 46 1208 1308
Fragments arranged by size:
3908 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 628 897 928 1729 2262 3299 3484 4262 4452
Size: 269 31 801 533 1037 185 778 190
Cuts at: 4452 5244 628
Size: 792 1854
Fragments arranged by size:
1854 1037 801 792 778 533 269 190
185 31
BsmI GAATG_Cn'
Cuts at: 748 2008 2037 748
Size: 1260 29 5181
Fragments arranged by size:
5181 1260 29
Bsp1286I G_dGCh'C
Cuts at: 158 628 897 928 1206 1384 1729 2262 3299
Size: 470 269 31 278 178 345 533 1037
Cuts at: 3299 3484 4095 4188 4262 4452 4514 5244 158
Size: 185 611 93 74 190 62 730 1384
Fragments arranged by size:
1384 1037 730 611 533 470 345 278
269 190 185 178 93 74 62 31
BspEI T'CCGG_A
Cuts at: 5690 5690
Size: 6470
BspHI T'CATG_A
Cuts at: 1032 2231 2963 5646 1032
Size: 1199 732 2683 1856
Fragments arranged by size:
2683 1856 1199 732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3033 3520 3734 4036 4417 3033
Size: 487 214 302 381 5086
Fragments arranged by size:
5086 487 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4762 4816 6300 6465 50
Size: 4712 54 1484 165 55
Fragments arranged by size:
4712 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 617 646 2177 2526 2611 2809 2996 4382 617
Size: 29 1531 349 85 198 187 1386 2705
Fragments arranged by size:
2705 1531 1386 349 198 187 85 29
BsrFI r'CCGG_y
Cuts at: 3254 4468 4649 4858 3254
Size: 1214 181 209 4866
Fragments arranged by size:
4866 1214 209 181
BsrGI T'GTAC_A
Cuts at: 1254 1254
Size: 6470
BssSI C'ACGA_G
Cuts at: 4741 5099 4741
Size: 358 6112
BstAPI GCAn_nnn'nTGC
Cuts at: 657 3847 657
Size: 3190 3280
BstBI TT'CG_AA
Cuts at: 4831 4831
Size: 6470
BstEII G'GTnAC_C
Cuts at: 1540 1540
Size: 6470
BstYI r'GATC_y
Cuts at: 1 2278 2793 3139 3264 3495 3962 3985 4320
Size: 2277 515 346 125 231 467 23 335
Cuts at: 4320 4566 5578 5664 5676 6022 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2277 1012 515 467 449 346 346 335
246 231 125 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 121 121
Size: 6470
BtgI C'CryG_G
Cuts at: 1673 1673
Size: 6470
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4420 4601 4764 4420
Size: 181 163 6126
Fragments arranged by size:
6126 181 163
BtsI GCAGTG_nn'
Cuts at: 1754 2605 3713 1754
Size: 851 1108 4511
Fragments arranged by size:
4511 1108 851
ClaI AT'CG_AT
Cuts at: 1121 1121
Size: 6470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 948 983 948
Size: 35 6435
DraI TTT'AAA
Cuts at: 3469 3469
Size: 6470
DrdI GACnn_nn'nnGTC
Cuts at: 4176 5034 4176
Size: 858 5612
EaeI y'GGCC_r
Cuts at: 4055 4229 4620 4647 5992 4055
Size: 174 391 27 1345 4533
Fragments arranged by size:
4533 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4055 4055
Size: 6470
EarI CTCTTCn'nnn_
Cuts at: 767 3022 4493 4703 767
Size: 2255 1471 210 2534
Fragments arranged by size:
2534 2255 1471 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4678 4988 5134 6280 4678
Size: 310 146 1146 4868
Fragments arranged by size:
4868 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2260 3297 3482 2260
Size: 1037 185 5248
Fragments arranged by size:
5248 1037 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 1148 1940 2784 2817 3280 4295 4727 5474
Size: 987 792 844 33 463 1015 432 747
Cuts at: 5474 5742 161
Size: 268 889
Fragments arranged by size:
1015 987 889 844 792 747 463 432
268 33
EcoNI CCTnn'n_nnAGG
Cuts at: 1494 1494
Size: 6470
EcoO109I rG'GnC_Cy
Cuts at: 1380 1381 3609 1380
Size: 1 2228 4241
Fragments arranged by size:
4241 2228 1
EcoRI G'AATT_C
Cuts at: 2110 2403 3474 2110
Size: 293 1071 5106
Fragments arranged by size:
5106 1071 293
EcoRV GAT'ATC
Cuts at: 983 2853 983
Size: 1870 4600
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1561 4489 1561
Size: 2928 3542
FspI TGC'GCA
Cuts at: 4251 4251
Size: 6470
HaeII r_GCGC'y
Cuts at: 138 3904 4152 5174 6153 138
Size: 3766 248 1022 979 455
Fragments arranged by size:
3766 1022 979 455 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 492 524 1432 1464 1929 1961 3094 3126 4312
Size: 32 908 32 465 32 1133 32 1186
Cuts at: 4312 4342 4344 4374 492
Size: 30 2 30 2588
Fragments arranged by size:
2588 1186 1133 908 465 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1014 3509 1014
Size: 2495 3975
HpaI GTT'AAC
Cuts at: 1014 1014
Size: 6470
KasI G'GCGC_C
Cuts at: 134 3900 4148 134
Size: 3766 248 2456
Fragments arranged by size:
3766 2456 248
KpnI G_GTAC'C
Cuts at: 3490 3490
Size: 6470
MfeI C'AATT_G
Cuts at: 2615 2954 2615
Size: 339 6131
MluI A'CGCG_T
Cuts at: 5903 5903
Size: 6470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2511 2675 5141 5325 2511
Size: 164 2466 184 3656
Fragments arranged by size:
3656 2466 184 164
MscI TGG'CCA
Cuts at: 4231 4231
Size: 6470
MslI CAynn'nnrTG
Cuts at: 520 610 2302 2560 3118 4459 4586 520
Size: 90 1692 258 558 1341 127 2404
Fragments arranged by size:
2404 1692 1341 558 258 127 90
MspA1I CmG'CkG
Cuts at: 610 3581 3895 4255 5268 5513 610
Size: 2971 314 360 1013 245 1567
Fragments arranged by size:
2971 1567 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4651 4651
Size: 6470
NarI GG'CG_CC
Cuts at: 135 3901 4149 135
Size: 3766 248 2456
Fragments arranged by size:
3766 2456 248
NcoI C'CATG_G
Cuts at: 1673 1673
Size: 6470
NdeI CA'TA_TG
Cuts at: 240 2264 240
Size: 2024 4446
NgoMIV G'CCGG_C
Cuts at: 4649 4649
Size: 6470
NheI G'CTAG_C
Cuts at: 1077 1077
Size: 6470
NsiI A_TGCA'T
Cuts at: 748 1368 2008 3021 748
Size: 620 640 1013 4197
Fragments arranged by size:
4197 1013 640 620
NspI r_CATG'y
Cuts at: 1370 2034 3019 3523 4023 4930 1370
Size: 664 985 504 500 907 2910
Fragments arranged by size:
2910 985 907 664 504 500
PasI CC'CwG_GG
Cuts at: 159 951 159
Size: 792 5678
PciI A'CATG_T
Cuts at: 4926 4926
Size: 6470
PfoI T'CCnGG_A
Cuts at: 3278 3278
Size: 6470
PmeI GTTT'AAAC
Cuts at: 3469 3469
Size: 6470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5609 5641 5609
Size: 32 6438
PspOMI G'GGCC_C
Cuts at: 1380 1380
Size: 6470
PstI C_TGCA'G
Cuts at: 321 2600 3517 4202 321
Size: 2279 917 685 2589
Fragments arranged by size:
2589 2279 917 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6470
PvuII CAG'CTG
Cuts at: 610 3895 4255 610
Size: 3285 360 2825
Fragments arranged by size:
3285 2825 360
RsrII CG'GwC_CG
Cuts at: 4665 4665
Size: 6470
SacI G_AGCT'C
Cuts at: 2262 3299 3484 2262
Size: 1037 185 5248
Fragments arranged by size:
5248 1037 185
SalI G'TCGA_C
Cuts at: 3507 3507
Size: 6470
SapI GCTCTTCn'nnn_
Cuts at: 767 4493 4703 767
Size: 3726 210 2534
Fragments arranged by size:
3726 2534 210
SbfI CC_TGCA'GG
Cuts at: 3517 3517
Size: 6470
ScaI AGT'ACT
Cuts at: 5987 5987
Size: 6470
SfcI C'TryA_G
Cuts at: 33 317 422 2596 2726 3513 4198 5191 5382
Size: 284 105 2174 130 787 685 993 191
Cuts at: 5382 33
Size: 1121
Fragments arranged by size:
2174 1121 993 787 685 284 191 130
105
SfoI GGC'GCC
Cuts at: 136 3902 4150 136
Size: 3766 248 2456
Fragments arranged by size:
3766 2456 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6470
SmaI CCC'GGG
Cuts at: 3492 3492
Size: 6470
SmlI C'TyrA_G
Cuts at: 214 559 1933 3457 3625 4540 5032 5294 214
Size: 345 1374 1524 168 915 492 262 1390
Fragments arranged by size:
1524 1390 1374 915 492 345 262 168
SpeI A'CTAG_T
Cuts at: 67 2371 67
Size: 2304 4166
SphI G_CATG'C
Cuts at: 3523 4023 3523
Size: 500 5970
SspI AAT'ATT
Cuts at: 1176 2224 2945 3239 1176
Size: 1048 721 294 4407
Fragments arranged by size:
4407 1048 721 294
StuI AGG'CCT
Cuts at: 1849 1849
Size: 6470
StyI C'CwwG_G
Cuts at: 1673 3604 3958 1673
Size: 1931 354 4185
Fragments arranged by size:
4185 1931 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 964 1334 4053 4186 4645 4854 964
Size: 370 2719 133 459 209 2580
Fragments arranged by size:
2719 2580 459 370 209 133
TatI w'GTAC_w
Cuts at: 1254 1348 5985 1254
Size: 94 4637 1739
Fragments arranged by size:
4637 1739 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5987 6019 5987
Size: 32 6438
Tth111I GACn'n_nGTC
Cuts at: 1828 4267 1828
Size: 2439 4031
XbaI T'CTAG_A
Cuts at: 3501 3501
Size: 6470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 744 3965 744
Size: 3221 3249
XmaI C'CCGG_G
Cuts at: 3490 3490
Size: 6470
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PasI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AloI AscI AvrII BaeI BcgI
BglI BmgBI BsaXI BsiWI BsmBI BssHII BstXI BstZ17I
DraIII FseI FspAI HindIII NotI NruI PacI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI