Restriction map for
pF1KB7350
(Circular) (Six-base) MAPSORT of: pF1KB7350.seq Check: 7834 from: 1 to: 6350
pF1KB7350 6350 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 805 1018 1405 2173 2791 805
Size: 213 387 768 618 4364
Fragments arranged by size:
4364 768 618 387 213
AccI GT'mk_AC
Cuts at: 3388 3388
Size: 6350
Acc65I G'GTAC_C
Cuts at: 3366 3366
Size: 6350
AclI AA'CG_TT
Cuts at: 6190 6190
Size: 6350
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 1777 1899 2181 2974 3247 4175 4607 5354
Size: 1051 122 282 793 273 928 432 747
Cuts at: 5354 5622 726
Size: 268 1454
Fragments arranged by size:
1454 1051 928 793 747 432 282 273
268 122
AfeI AGC'GCT
Cuts at: 473 1291 473
Size: 818 5532
AflIII A'CryG_T
Cuts at: 1010 3702 4806 5783 1010
Size: 2692 1104 977 1577
Fragments arranged by size:
2692 1577 1104 977
AgeI A'CCGG_T
Cuts at: 4738 4738
Size: 6350
AhdI GACnn_n'nnGTC
Cuts at: 1803 1803
Size: 6350
AleI CACnn'nnGTG
Cuts at: 2893 2893
Size: 6350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2381 2595 2381
Size: 214 6136
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2503 2535 2503
Size: 32 6318
AlwNI CAG_nnn'CTG
Cuts at: 441 680 774 1335 1538 1629 2697 3655 5222
Size: 239 94 561 203 91 1068 958 1567
Cuts at: 5222 441
Size: 1569
Fragments arranged by size:
1569 1567 1068 958 561 239 203 94
91
ApaI G_GGCC'C
Cuts at: 363 2615 363
Size: 2252 4098
ApaLI G'TGCA_C
Cuts at: 5120 5120
Size: 6350
ApoI r'AATT_y
Cuts at: 633 3354 5703 5959 633
Size: 2721 2349 256 1024
Fragments arranged by size:
2721 2349 1024 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6350
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6350
AvaI C'yCGr_G
Cuts at: 757 814 3370 757
Size: 57 2556 3737
Fragments arranged by size:
3737 2556 57
BamHI G'GATC_C
Cuts at: 818 3375 818
Size: 2557 3793
BanI G'GyrC_C
Cuts at: 917 1092 1617 2306 2590 3366 3780 4028 4063
Size: 175 525 689 284 776 414 248 35
Cuts at: 4063 917
Size: 3204
Fragments arranged by size:
3204 776 689 525 414 284 248 175
35
BanII G_rGCy'C
Cuts at: 363 931 1222 1755 2615 2819 3364 4394 363
Size: 568 291 533 860 204 545 1030 2319
Fragments arranged by size:
2319 1030 860 568 545 533 291 204
BbeI G_GCGC'C
Cuts at: 2594 3784 4032 2594
Size: 1190 248 4912
Fragments arranged by size:
4912 1190 248
BbsI GAAGACnn'nnnn_
Cuts at: 732 2247 3141 732
Size: 1515 894 3941
Fragments arranged by size:
3941 1515 894
BbvCI CC'TCA_GC
Cuts at: 558 997 1610 3271 558
Size: 439 613 1661 3637
Fragments arranged by size:
3637 1661 613 439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1839 1873 1839
Size: 34 6316
BciVI GTATCCnnnnn_n'
Cuts at: 4248 5009 6350 4248
Size: 761 1341 4248
Fragments arranged by size:
4248 1341 761
BclI T'GATC_A
Cuts at: 3020 3870 3020
Size: 850 5500
BglI GCCn_nnn'nGGC
Cuts at: 656 656
Size: 6350
BglII A'GATC_T
Cuts at: 2516 3865 5902 2516
Size: 1349 2037 2964
Fragments arranged by size:
2964 2037 1349
BlpI GC'TnA_GC
Cuts at: 862 1171 1690 3462 862
Size: 309 519 1772 3750
Fragments arranged by size:
3750 1772 519 309
Bme1580I G_kGCm'C
Cuts at: 363 1097 1894 2615 2788 3334 3975 4068 5124
Size: 734 797 721 173 546 641 93 1056
Cuts at: 5124 363
Size: 1589
Fragments arranged by size:
1589 1056 797 734 721 641 546 173
93
BmrI ACTGGGnnnn_n'
Cuts at: 212 926 1032 2800 3562 3643 3978 6122 212
Size: 714 106 1768 762 81 335 2144 440
Fragments arranged by size:
2144 1768 762 714 440 335 106 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 887 1278 1428 3114 4717 887
Size: 391 150 1686 1603 2520
Fragments arranged by size:
2520 1686 1603 391 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 516 1236 1244 2591 3124 434
Size: 82 720 8 1347 533 3660
Fragments arranged by size:
3660 1347 720 533 82 8
Bpu10I CC'TnA_GC
Cuts at: 558 901 997 1610 3271 558
Size: 343 96 613 1661 3637
Fragments arranged by size:
3637 1661 613 343 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2813 3526 4405 4897 5195 2414
Size: 399 713 879 492 298 3569
Fragments arranged by size:
3569 879 713 492 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 204 483 1811 1864 2958 6054 204
Size: 279 1328 53 1094 3096 500
Fragments arranged by size:
3096 1328 1094 500 279 53
BsaAI yAC'GTr
Cuts at: 1313 1449 1707 3703 1313
Size: 136 258 1996 3960
Fragments arranged by size:
3960 1996 258 136
BsaBI GATnn'nnATC
Cuts at: 6 3864 5901 6
Size: 3858 2037 455
Fragments arranged by size:
3858 2037 455
BsaHI Gr'CG_yC
Cuts at: 1409 2591 3146 3781 4029 4731 6227 1409
Size: 1182 555 635 248 702 1496 1532
Fragments arranged by size:
1532 1496 1182 702 635 555 248
BsaWI w'CCGG_w
Cuts at: 2400 3539 4060 4738 5012 5159 5570 2400
Size: 1139 521 678 274 147 411 3180
Fragments arranged by size:
3180 1139 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 119 149 530 560 817 847 918 948 1485
Size: 30 381 30 257 30 71 30 537
Cuts at: 1485 1515 1966 1996 2503 2533 119
Size: 30 451 30 507 30 3936
Fragments arranged by size:
3936 537 507 451 381 257 71 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 406 1506 1627 3306 403
Size: 3 1100 121 1679 3447
Fragments arranged by size:
3447 1679 1100 121 3
BseYI C'CCAG_C
Cuts at: 159 836 1186 2127 2988 3012 3069 3229 3775
Size: 677 350 941 861 24 57 160 546
Cuts at: 3775 4533 5110 5606 159
Size: 758 577 496 903
Fragments arranged by size:
941 903 861 758 677 577 546 496
350 160 57 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1524 1524
Size: 6350
BsiEI CG_ry'CG
Cuts at: 104 3892 3938 5146 104
Size: 3788 46 1208 1308
Fragments arranged by size:
3788 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1421 1696 2771 3364 4142 4332 5124 1421
Size: 275 1075 593 778 190 792 2647
Fragments arranged by size:
2647 1075 792 778 593 275 190
BsiWI C'GTAC_G
Cuts at: 1313 1313
Size: 6350
BsmI GAATG_Cn'
Cuts at: 2860 3302 2860
Size: 442 5908
BsmBI CGTCTCn'nnnn_
Cuts at: 1008 1008
Size: 6350
Bsp1286I G_dGCh'C
Cuts at: 363 931 1097 1222 1421 1696 1755 1894 2615
Size: 568 166 125 199 275 59 139 721
Cuts at: 2615 2771 2788 2819 3334 3364 3975 4068 4142
Size: 156 17 31 515 30 611 93 74
Cuts at: 4142 4332 4394 5124 363
Size: 190 62 730 1589
Fragments arranged by size:
1589 730 721 611 568 515 275 199
190 166 156 139 125 93 74 62
59 31 30 17
BspEI T'CCGG_A
Cuts at: 2400 5570 2400
Size: 3170 3180
BspHI T'CATG_A
Cuts at: 5526 5526
Size: 6350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1018 1334 1405 2173 2791 3400 3614 3916
Size: 213 316 71 768 618 609 214 302
Cuts at: 3916 4297 805
Size: 381 2858
Fragments arranged by size:
2858 768 618 609 381 316 302 214
213 71
BsrBI CCG'CTC
Cuts at: 50 2413 2625 4642 4696 6180 6345 50
Size: 2363 212 2017 54 1484 165 55
Fragments arranged by size:
2363 2017 1484 212 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4262 4262
Size: 6350
BsrFI r'CCGG_y
Cuts at: 1524 4348 4529 4738 1524
Size: 2824 181 209 3136
Fragments arranged by size:
3136 2824 209 181
BsrGI T'GTAC_A
Cuts at: 489 489
Size: 6350
BssSI C'ACGA_G
Cuts at: 4621 4979 4621
Size: 358 5992
BstAPI GCAn_nnn'nTGC
Cuts at: 1889 3727 1889
Size: 1838 4512
BstBI TT'CG_AA
Cuts at: 4711 4711
Size: 6350
BstEII G'GTnAC_C
Cuts at: 164 2185 164
Size: 2021 4329
BstXI CCAn_nnnn'nTGG
Cuts at: 454 727 1719 1951 3246 454
Size: 273 992 232 1295 3558
Fragments arranged by size:
3558 1295 992 273 232
BstYI r'GATC_y
Cuts at: 1 427 818 1625 2516 3266 3375 3842 3865
Size: 426 391 807 891 750 109 467 23
Cuts at: 3865 4200 4446 5458 5544 5556 5902 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 891 807 750 467 449 426 391
346 335 246 109 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 913 1403 2842 913
Size: 490 1439 4421
Fragments arranged by size:
4421 1439 490
BtgI C'CryG_G
Cuts at: 107 981 1208 2942 3101 3307 107
Size: 874 227 1734 159 206 3150
Fragments arranged by size:
3150 1734 874 227 206 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4300 4481 4644 4300
Size: 181 163 6006
Fragments arranged by size:
6006 181 163
BtsI GCAGTG_nn'
Cuts at: 127 2002 2336 2363 3593 127
Size: 1875 334 27 1230 2884
Fragments arranged by size:
2884 1875 1230 334 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 706 741 2777 2812 706
Size: 35 2036 35 4244
Fragments arranged by size:
4244 2036 35 35
DraI TTT'AAA
Cuts at: 3349 3349
Size: 6350
DrdI GACnn_nn'nnGTC
Cuts at: 963 4056 4914 963
Size: 3093 858 2399
Fragments arranged by size:
3093 2399 858
EaeI y'GGCC_r
Cuts at: 140 330 984 1443 1709 2321 2567 2666 2909
Size: 190 654 459 266 612 246 99 243
Cuts at: 2909 2945 3077 3935 4109 4500 4527 5872 140
Size: 36 132 858 174 391 27 1345 618
Fragments arranged by size:
1345 858 654 618 612 459 391 266
246 243 190 174 132 99 36 27
EagI C'GGCC_G
Cuts at: 3935 3935
Size: 6350
EarI CTCTTCn'nnn_
Cuts at: 155 573 587 2278 4373 4583 155
Size: 418 14 1691 2095 210 1922
Fragments arranged by size:
2095 1922 1691 418 210 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2518 4558 4868 5014 6160 2518
Size: 2040 310 146 1146 2708
Fragments arranged by size:
2708 2040 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3362 3362
Size: 6350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 516 726 1236 1244 1777 1899 2181 2591
Size: 82 210 510 8 533 122 282 410
Cuts at: 2591 2974 3124 3247 4175 4607 5354 5622 434
Size: 383 150 123 928 432 747 268 1162
Fragments arranged by size:
1162 928 747 533 510 432 410 383
282 268 210 150 123 122 82 8
EcoNI CCTnn'n_nnAGG
Cuts at: 385 1078 2845 385
Size: 693 1767 3890
Fragments arranged by size:
3890 1767 693
EcoO109I rG'GnC_Cy
Cuts at: 244 785 1356 1458 1785 2611 2612 2694 2983
Size: 541 571 102 327 826 1 82 289
Cuts at: 2983 3489 244
Size: 506 3105
Fragments arranged by size:
3105 826 571 541 506 327 289 102
82 1
EcoRI G'AATT_C
Cuts at: 3354 3354
Size: 6350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4369 4369
Size: 6350
FspI TGC'GCA
Cuts at: 3202 4131 3202
Size: 929 5421
HaeII r_GCGC'y
Cuts at: 475 1293 2422 2461 2594 3003 3784 4032 5054
Size: 818 1129 39 133 409 781 248 1022
Cuts at: 5054 6033 475
Size: 979 792
Fragments arranged by size:
1129 1022 979 818 792 781 409 248
133 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 528 560 861 893 918 950 1789 1821 2198
Size: 32 301 32 25 32 839 32 377
Cuts at: 2198 2230 4192 4222 4224 4254 528
Size: 32 1962 30 2 30 2624
Fragments arranged by size:
2624 1962 839 377 301 32 32 32
32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 621 3389 621
Size: 2768 3582
HindIII A'AGCT_T
Cuts at: 1238 3194 1238
Size: 1956 4394
KasI G'GCGC_C
Cuts at: 2590 3780 4028 2590
Size: 1190 248 4912
Fragments arranged by size:
4912 1190 248
KpnI G_GTAC'C
Cuts at: 3370 3370
Size: 6350
MluI A'CGCG_T
Cuts at: 5783 5783
Size: 6350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1697 2843 5021 5205 1031
Size: 666 1146 2178 184 2176
Fragments arranged by size:
2178 2176 1146 666 184
MscI TGG'CCA
Cuts at: 142 1445 1711 2323 2668 2911 2947 3079 4111
Size: 1303 266 612 345 243 36 132 1032
Cuts at: 4111 142
Size: 2381
Fragments arranged by size:
2381 1303 1032 612 345 266 243 132
36
MslI CAynn'nnrTG
Cuts at: 2893 2920 2947 4339 4466 2893
Size: 27 27 1392 127 4777
Fragments arranged by size:
4777 1392 127 27 27
MspA1I CmG'CkG
Cuts at: 840 1134 1170 1210 1538 1638 1947 2715 2736
Size: 294 36 40 328 100 309 768 21
Cuts at: 2736 3275 3461 3775 4135 5148 5393 840
Size: 539 186 314 360 1013 245 1797
Fragments arranged by size:
1797 1013 768 539 360 328 314 309
294 245 186 100 40 36 21
NaeI GCC'GGC
Cuts at: 4531 4531
Size: 6350
NarI GG'CG_CC
Cuts at: 2591 3781 4029 2591
Size: 1190 248 4912
Fragments arranged by size:
4912 1190 248
NcoI C'CATG_G
Cuts at: 107 2942 3307 107
Size: 2835 365 3150
Fragments arranged by size:
3150 2835 365
NgoMIV G'CCGG_C
Cuts at: 4529 4529
Size: 6350
NspI r_CATG'y
Cuts at: 1014 1883 3294 3403 3903 4810 1014
Size: 869 1411 109 500 907 2554
Fragments arranged by size:
2554 1411 907 869 500 109
PasI CC'CwG_GG
Cuts at: 215 922 1056 1838 2811 215
Size: 707 134 782 973 3754
Fragments arranged by size:
3754 973 782 707 134
PciI A'CATG_T
Cuts at: 1010 4806 1010
Size: 3796 2554
PfoI T'CCnGG_A
Cuts at: 440 532 1379 440
Size: 92 847 5411
Fragments arranged by size:
5411 847 92
PmeI GTTT'AAAC
Cuts at: 3349 3349
Size: 6350
PmlI CAC'GTG
Cuts at: 1449 1449
Size: 6350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2535 5489 5521 2503
Size: 32 2954 32 3332
Fragments arranged by size:
3332 2954 32 32
PpuMI rG'GwC_Cy
Cuts at: 244 785 1356 1785 2983 244
Size: 541 571 429 1198 3611
Fragments arranged by size:
3611 1198 571 541 429
PspOMI G'GGCC_C
Cuts at: 359 2611 359
Size: 2252 4098
PstI C_TGCA'G
Cuts at: 230 810 845 950 1331 1589 1940 2009 3397
Size: 580 35 105 381 258 351 69 1388
Cuts at: 3397 4082 230
Size: 685 2498
Fragments arranged by size:
2498 1388 685 580 381 351 258 105
69 35
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6350
PvuII CAG'CTG
Cuts at: 840 1134 1170 1538 1638 1947 2715 2736 3275
Size: 294 36 368 100 309 768 21 539
Cuts at: 3275 3775 4135 840
Size: 500 360 3055
Fragments arranged by size:
3055 768 539 500 368 360 309 294
100 36 21
RsrII CG'GwC_CG
Cuts at: 4545 4545
Size: 6350
SacI G_AGCT'C
Cuts at: 3364 3364
Size: 6350
SacII CC_GC'GG
Cuts at: 1211 1211
Size: 6350
SalI G'TCGA_C
Cuts at: 3387 3387
Size: 6350
SanDI GG'GwC_CC
Cuts at: 244 785 1356 2983 244
Size: 541 571 1627 3611
Fragments arranged by size:
3611 1627 571 541
SapI GCTCTTCn'nnn_
Cuts at: 4373 4583 4373
Size: 210 6140
SbfI CC_TGCA'GG
Cuts at: 3397 3397
Size: 6350
ScaI AGT'ACT
Cuts at: 5867 5867
Size: 6350
SexAI A'CCwGG_T
Cuts at: 1801 1801
Size: 6350
SfcI C'TryA_G
Cuts at: 33 226 806 841 946 1327 1585 1936 1974
Size: 193 580 35 105 381 258 351 38
Cuts at: 1974 2005 3393 4078 5071 5262 33
Size: 31 1388 685 993 191 1121
Fragments arranged by size:
1388 1121 993 685 580 381 351 258
193 191 105 38 35 31
SfoI GGC'GCC
Cuts at: 2592 3782 4030 2592
Size: 1190 248 4912
Fragments arranged by size:
4912 1190 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6350
SmaI CCC'GGG
Cuts at: 759 816 3372 759
Size: 57 2556 3737
Fragments arranged by size:
3737 2556 57
SmlI C'TyrA_G
Cuts at: 2429 2828 3505 4420 4912 5174 2429
Size: 399 677 915 492 262 3605
Fragments arranged by size:
3605 915 677 492 399 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6350
SphI G_CATG'C
Cuts at: 1883 3403 3903 1883
Size: 1520 500 4330
Fragments arranged by size:
4330 1520 500
StuI AGG'CCT
Cuts at: 579 579
Size: 6350
StyI C'CwwG_G
Cuts at: 107 341 574 1436 1712 2942 3307 3484 3838
Size: 234 233 862 276 1230 365 177 354
Cuts at: 3838 107
Size: 2619
Fragments arranged by size:
2619 1230 862 365 354 276 234 233
177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 935 2464 3060 3933 4066 4525 4734 935
Size: 1529 596 873 133 459 209 2551
Fragments arranged by size:
2551 1529 873 596 459 209 133
TatI w'GTAC_w
Cuts at: 489 1034 2480 5865 489
Size: 545 1446 3385 974
Fragments arranged by size:
3385 1446 974 545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 119 135 151 167 530 562 1340 1372 1485
Size: 16 16 16 363 32 778 32 113
Cuts at: 1485 1517 5867 5899 119
Size: 32 4350 32 570
Fragments arranged by size:
4350 778 570 363 113 32 32 32
32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 404 1513 1783 4147 404
Size: 1109 270 2364 2607
Fragments arranged by size:
2607 2364 1109 270
XbaI T'CTAG_A
Cuts at: 3381 3381
Size: 6350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 512 724 1194 1704 2028 2154 3243 3845 512
Size: 212 470 510 324 126 1089 602 3017
Fragments arranged by size:
3017 1089 602 510 470 324 212 126
XmaI C'CCGG_G
Cuts at: 757 814 3370 757
Size: 57 2556 3737
Fragments arranged by size:
3737 2556 57
XmnI GAAnn'nnTTC
Cuts at: 3302 3302
Size: 6350
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BfrBI BmgBI BmtI
BssHII BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI