Restriction map for
pF1KB7355
(Circular) (Six-base) MAPSORT of: pF1KB7355.seq Check: 7832 from: 1 to: 6377
pF1KB7355 6377 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 1581 1581
Size: 6377
AccI GT'mk_AC
Cuts at: 1595 3415 1595
Size: 1820 4557
Acc65I G'GTAC_C
Cuts at: 3393 3393
Size: 6377
AclI AA'CG_TT
Cuts at: 6217 6217
Size: 6377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 599 709 1184 1930 2073 2347 2481 3207
Size: 122 110 475 746 143 274 134 726
Cuts at: 3207 4202 4634 5381 5649 477
Size: 995 432 747 268 1205
Fragments arranged by size:
1205 995 747 746 726 475 432 274
268 143 134 122 110
AfeI AGC'GCT
Cuts at: 2117 2117
Size: 6377
AflIII A'CryG_T
Cuts at: 301 1474 2398 3729 4833 5810 301
Size: 1173 924 1331 1104 977 868
Fragments arranged by size:
1331 1173 1104 977 924 868
AgeI A'CCGG_T
Cuts at: 4765 4765
Size: 6377
AleI CACnn'nnGTG
Cuts at: 2036 2036
Size: 6377
AlwNI CAG_nnn'CTG
Cuts at: 2541 2906 3255 3682 5249 2541
Size: 365 349 427 1567 3669
Fragments arranged by size:
3669 1567 427 365 349
ApaLI G'TGCA_C
Cuts at: 1443 5147 1443
Size: 3704 2673
ApoI r'AATT_y
Cuts at: 1090 1216 2014 2161 2169 2460 2486 3381 5730
Size: 126 798 147 8 291 26 895 2349
Cuts at: 5730 5986 1090
Size: 256 1481
Fragments arranged by size:
2349 1481 895 798 291 256 147 126
26 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 6377
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6377
AvaI C'yCGr_G
Cuts at: 3397 3397
Size: 6377
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2876 2909 2876
Size: 33 6344
BamHI G'GATC_C
Cuts at: 3402 3402
Size: 6377
BanI G'GyrC_C
Cuts at: 3393 3807 4055 4090 3393
Size: 414 248 35 5680
Fragments arranged by size:
5680 414 248 35
BanII G_rGCy'C
Cuts at: 3131 3391 4421 3131
Size: 260 1030 5087
Fragments arranged by size:
5087 1030 260
BbeI G_GCGC'C
Cuts at: 3811 4059 3811
Size: 248 6129
BbsI GAAGACnn'nnnn_
Cuts at: 483 2649 3092 483
Size: 2166 443 3768
Fragments arranged by size:
3768 2166 443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1489 1523 1489
Size: 34 6343
BciVI GTATCCnnnnn_n'
Cuts at: 1037 1701 3120 4275 5036 6377 1037
Size: 664 1419 1155 761 1341 1037
Fragments arranged by size:
1419 1341 1155 1037 761 664
BclI T'GATC_A
Cuts at: 1794 3897 1794
Size: 2103 4274
BglII A'GATC_T
Cuts at: 2139 2274 3892 5929 2139
Size: 135 1618 2037 2587
Fragments arranged by size:
2587 2037 1618 135
BlpI GC'TnA_GC
Cuts at: 284 3489 284
Size: 3205 3172
Bme1580I G_kGCm'C
Cuts at: 1447 4002 4095 5151 1447
Size: 2555 93 1056 2673
Fragments arranged by size:
2673 2555 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1221 1920 3210 3589 3670 4005 6149 1221
Size: 699 1290 379 81 335 2144 1449
Fragments arranged by size:
2144 1449 1290 699 379 335 81
BmtI G_CTAG'C
Cuts at: 682 682
Size: 6377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4744 4744
Size: 6377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 2680 2883 511
Size: 2169 203 4005
Fragments arranged by size:
4005 2169 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2572 2602 3553 4432 4924 5222 2572
Size: 30 951 879 492 298 3727
Fragments arranged by size:
3727 951 879 492 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 6081 6081
Size: 6377
BsaAI yAC'GTr
Cuts at: 384 3730 384
Size: 3346 3031
BsaBI GATnn'nnATC
Cuts at: 6 358 3891 5928 6
Size: 352 3533 2037 455
Fragments arranged by size:
3533 2037 455 352
BsaHI Gr'CG_yC
Cuts at: 1578 2654 3808 4056 4758 6254 1578
Size: 1076 1154 248 702 1496 1701
Fragments arranged by size:
1701 1496 1154 1076 702 248
BsaWI w'CCGG_w
Cuts at: 1711 2750 3566 4087 4765 5039 5186 5597 1711
Size: 1039 816 521 678 274 147 411 2491
Fragments arranged by size:
2491 1039 816 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1127 1157 1426 1456 1127
Size: 30 269 30 6048
Fragments arranged by size:
6048 269 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2886 2886
Size: 6377
BseYI C'CCAG_C
Cuts at: 1131 2450 3802 4560 5137 5633 1131
Size: 1319 1352 758 577 496 1875
Fragments arranged by size:
1875 1352 1319 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 862 1510 100
Size: 762 648 4967
Fragments arranged by size:
4967 762 648
BsiEI CG_ry'CG
Cuts at: 104 3919 3965 5173 104
Size: 3815 46 1208 1308
Fragments arranged by size:
3815 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1447 1482 3391 4169 4359 5151 1447
Size: 35 1909 778 190 792 2673
Fragments arranged by size:
2673 1909 792 778 190 35
BsmI GAATG_Cn'
Cuts at: 2373 2373
Size: 6377
BsmBI CGTCTCn'nnnn_
Cuts at: 1867 1867
Size: 6377
Bsp1286I G_dGCh'C
Cuts at: 1447 1482 3131 3391 4002 4095 4169 4359 4421
Size: 35 1649 260 611 93 74 190 62
Cuts at: 4421 5151 1447
Size: 730 2673
Fragments arranged by size:
2673 1649 730 611 260 190 93 74
62 35
BspEI T'CCGG_A
Cuts at: 5597 5597
Size: 6377
BspHI T'CATG_A
Cuts at: 1086 1880 3065 5553 1086
Size: 794 1185 2488 1910
Fragments arranged by size:
2488 1910 1185 794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 747 3427 3641 3943 4324 747
Size: 2680 214 302 381 2800
Fragments arranged by size:
2800 2680 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4669 4723 6207 6372 50
Size: 4619 54 1484 165 55
Fragments arranged by size:
4619 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 437 4289 437
Size: 3852 2525
BsrFI r'CCGG_y
Cuts at: 4375 4556 4765 4375
Size: 181 209 5987
Fragments arranged by size:
5987 209 181
BsrGI T'GTAC_A
Cuts at: 2401 2401
Size: 6377
BssSI C'ACGA_G
Cuts at: 2034 4648 5006 2034
Size: 2614 358 3405
Fragments arranged by size:
3405 2614 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3754 3754
Size: 6377
BstBI TT'CG_AA
Cuts at: 4738 4738
Size: 6377
BstXI CCAn_nnnn'nTGG
Cuts at: 857 857
Size: 6377
BstYI r'GATC_y
Cuts at: 1 891 2139 2274 2446 2537 3402 3869 3892
Size: 890 1248 135 172 91 865 467 23
Cuts at: 3892 4227 4473 5485 5571 5583 5929 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1248 1012 890 865 467 449 346 335
246 172 135 91 86 23 12
BstZ17I GTA'TAC
Cuts at: 1596 1596
Size: 6377
Bsu36I CC'TnA_GG
Cuts at: 1058 1058
Size: 6377
BtgI C'CryG_G
Cuts at: 107 107
Size: 6377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4327 4508 4671 4327
Size: 181 163 6033
Fragments arranged by size:
6033 181 163
BtsI GCAGTG_nn'
Cuts at: 543 3336 3620 543
Size: 2793 284 3300
Fragments arranged by size:
3300 2793 284
DraI TTT'AAA
Cuts at: 695 719 1115 1985 2232 3376 695
Size: 24 396 870 247 1144 3696
Fragments arranged by size:
3696 1144 870 396 247 24
DrdI GACnn_nn'nnGTC
Cuts at: 487 978 2926 4083 4941 487
Size: 491 1948 1157 858 1923
Fragments arranged by size:
1948 1923 1157 858 491
EaeI y'GGCC_r
Cuts at: 3962 4136 4527 4554 5899 3962
Size: 174 391 27 1345 4440
Fragments arranged by size:
4440 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3962 3962
Size: 6377
EarI CTCTTCn'nnn_
Cuts at: 1487 1949 2500 2944 3300 4400 4610 1487
Size: 462 551 444 356 1100 210 3254
Fragments arranged by size:
3254 1100 551 462 444 356 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4585 4895 5041 6187 4585
Size: 310 146 1146 4775
Fragments arranged by size:
4775 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3389 3389
Size: 6377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 511 599 709 1184 1930 2073 2347 2481
Size: 34 88 110 475 746 143 274 134
Cuts at: 2481 2680 2883 3207 4202 4634 5381 5649 477
Size: 199 203 324 995 432 747 268 1205
Fragments arranged by size:
1205 995 747 746 475 432 324 274
268 203 199 143 134 110 88 34
EcoO109I rG'GnC_Cy
Cuts at: 980 3207 3516 980
Size: 2227 309 3841
Fragments arranged by size:
3841 2227 309
EcoRI G'AATT_C
Cuts at: 2486 3381 2486
Size: 895 5482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1597 2643 4396 1597
Size: 1046 1753 3578
Fragments arranged by size:
3578 1753 1046
FspI TGC'GCA
Cuts at: 3328 4158 3328
Size: 830 5547
HaeII r_GCGC'y
Cuts at: 115 2119 3811 4059 5081 6060 115
Size: 2004 1692 248 1022 979 432
Fragments arranged by size:
2004 1692 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 548 580 1110 1142 1464 1496 2124 2156 2458
Size: 32 530 32 322 32 628 32 302
Cuts at: 2458 2490 4219 4249 4251 4281 548
Size: 32 1729 30 2 30 2644
Fragments arranged by size:
2644 1729 628 530 322 302 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 250 3416 250
Size: 3166 3211
KasI G'GCGC_C
Cuts at: 3807 4055 3807
Size: 248 6129
KpnI G_GTAC'C
Cuts at: 3397 3397
Size: 6377
MluI A'CGCG_T
Cuts at: 5810 5810
Size: 6377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 874 1337 1412 3073 5048 5232 874
Size: 463 75 1661 1975 184 2019
Fragments arranged by size:
2019 1975 1661 463 184 75
MscI TGG'CCA
Cuts at: 4138 4138
Size: 6377
MslI CAynn'nnrTG
Cuts at: 948 1375 1391 2036 2976 4366 4493 948
Size: 427 16 645 940 1390 127 2832
Fragments arranged by size:
2832 1390 940 645 427 127 16
MspA1I CmG'CkG
Cuts at: 1131 2903 3488 3802 4162 5175 5420 1131
Size: 1772 585 314 360 1013 245 2088
Fragments arranged by size:
2088 1772 1013 585 360 314 245
NaeI GCC'GGC
Cuts at: 4558 4558
Size: 6377
NarI GG'CG_CC
Cuts at: 3808 4056 3808
Size: 248 6129
NcoI C'CATG_G
Cuts at: 107 107
Size: 6377
NdeI CA'TA_TG
Cuts at: 1785 2804 1785
Size: 1019 5358
NgoMIV G'CCGG_C
Cuts at: 4556 4556
Size: 6377
NheI G'CTAG_C
Cuts at: 678 678
Size: 6377
NspI r_CATG'y
Cuts at: 305 686 1478 2402 3430 3930 4837 305
Size: 381 792 924 1028 500 907 1845
Fragments arranged by size:
1845 1028 924 907 792 500 381
PacI TTA_AT'TAA
Cuts at: 1028 1028
Size: 6377
PciI A'CATG_T
Cuts at: 301 1474 2398 4833 301
Size: 1173 924 2435 1845
Fragments arranged by size:
2435 1845 1173 924
PflMI CCAn_nnn'nTGG
Cuts at: 129 1543 129
Size: 1414 4963
PfoI T'CCnGG_A
Cuts at: 872 2780 872
Size: 1908 4469
PmeI GTTT'AAAC
Cuts at: 3376 3376
Size: 6377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5516 5548 5516
Size: 32 6345
PpuMI rG'GwC_Cy
Cuts at: 980 3207 980
Size: 2227 4150
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1259 1291 1259
Size: 32 6345
PstI C_TGCA'G
Cuts at: 825 3047 3424 4109 825
Size: 2222 377 685 3093
Fragments arranged by size:
3093 2222 685 377
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6377
PvuII CAG'CTG
Cuts at: 1131 2903 3802 4162 1131
Size: 1772 899 360 3346
Fragments arranged by size:
3346 1772 899 360
RsrII CG'GwC_CG
Cuts at: 4572 4572
Size: 6377
SacI G_AGCT'C
Cuts at: 3391 3391
Size: 6377
SalI G'TCGA_C
Cuts at: 3414 3414
Size: 6377
SapI GCTCTTCn'nnn_
Cuts at: 1487 4400 4610 1487
Size: 2913 210 3254
Fragments arranged by size:
3254 2913 210
SbfI CC_TGCA'GG
Cuts at: 3424 3424
Size: 6377
ScaI AGT'ACT
Cuts at: 5894 5894
Size: 6377
SexAI A'CCwGG_T
Cuts at: 593 593
Size: 6377
SfcI C'TryA_G
Cuts at: 33 821 1204 3043 3420 4105 5098 5289 33
Size: 788 383 1839 377 685 993 191 1121
Fragments arranged by size:
1839 1121 993 788 685 383 377 191
SfoI GGC'GCC
Cuts at: 3809 4057 3809
Size: 248 6129
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6377
SmaI CCC'GGG
Cuts at: 3399 3399
Size: 6377
SmlI C'TyrA_G
Cuts at: 2587 2617 3532 4447 4939 5201 2587
Size: 30 915 915 492 262 3763
Fragments arranged by size:
3763 915 915 492 262 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6377
SphI G_CATG'C
Cuts at: 686 3430 3930 686
Size: 2744 500 3133
Fragments arranged by size:
3133 2744 500
SspI AAT'ATT
Cuts at: 2216 2216
Size: 6377
StuI AGG'CCT
Cuts at: 1339 3117 1339
Size: 1778 4599
StyI C'CwwG_G
Cuts at: 107 1014 2547 3511 3865 107
Size: 907 1533 964 354 2619
Fragments arranged by size:
2619 1533 964 907 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2134 3298 3960 4093 4552 4761 2134
Size: 1164 662 133 459 209 3750
Fragments arranged by size:
3750 1164 662 459 209 133
TatI w'GTAC_w
Cuts at: 151 1639 2401 2691 2835 5892 151
Size: 1488 762 290 144 3057 636
Fragments arranged by size:
3057 1488 762 636 290 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1426 1458 5894 5926 1426
Size: 32 4436 32 1877
Fragments arranged by size:
4436 1877 32 32
Tth111I GACn'n_nGTC
Cuts at: 4174 4174
Size: 6377
XbaI T'CTAG_A
Cuts at: 607 1869 3408 607
Size: 1262 1539 3576
Fragments arranged by size:
3576 1539 1262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3120 3872 3120
Size: 752 5625
XmaI C'CCGG_G
Cuts at: 3397 3397
Size: 6377
XmnI GAAnn'nnTTC
Cuts at: 3151 3151
Size: 6377
ZraI GAC'GTC
Cuts at: 1579 1579
Size: 6377
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PacI PciI PflMI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI ApaI AscI AvrII
BbvCI BfrBI BglI BmgBI Bpu10I BsiWI BssHII BstEII
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NotI NruI NsiI PasI PmlI PshAI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI