Restriction map for
pF1KB7359
(Circular) (Six-base) MAPSORT of: pF1KB7359.seq Check: 8292 from: 1 to: 4130
pF1KB7359 4130 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1168 1168
Size: 4130
Acc65I G'GTAC_C
Cuts at: 1146 1146
Size: 4130
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1955 2387 3134 3402 1955
Size: 432 747 268 2683
Fragments arranged by size:
2683 747 432 268
AflIII A'CryG_T
Cuts at: 416 1482 2586 3563 416
Size: 1066 1104 977 983
Fragments arranged by size:
1104 1066 983 977
AgeI A'CCGG_T
Cuts at: 2518 2518
Size: 4130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 527 884 319
Size: 208 357 3565
Fragments arranged by size:
3565 357 208
AlwNI CAG_nnn'CTG
Cuts at: 1435 3002 1435
Size: 1567 2563
ApaLI G'TGCA_C
Cuts at: 168 2900 168
Size: 2732 1398
ApoI r'AATT_y
Cuts at: 520 833 1107 1134 3483 3739 520
Size: 313 274 27 2349 256 911
Fragments arranged by size:
2349 911 313 274 256 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 1150 1150
Size: 4130
BamHI G'GATC_C
Cuts at: 1155 1155
Size: 4130
BanI G'GyrC_C
Cuts at: 1146 1560 1808 1843 1146
Size: 414 248 35 3433
Fragments arranged by size:
3433 414 248 35
BanII G_rGCy'C
Cuts at: 1144 2174 1144
Size: 1030 3100
BbeI G_GCGC'C
Cuts at: 1564 1812 1564
Size: 248 3882
BbsI GAAGACnn'nnnn_
Cuts at: 723 732 723
Size: 9 4121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 483 517 483
Size: 34 4096
BciVI GTATCCnnnnn_n'
Cuts at: 2028 2789 4130 2028
Size: 761 1341 2028
Fragments arranged by size:
2028 1341 761
BclI T'GATC_A
Cuts at: 1650 1650
Size: 4130
BglII A'GATC_T
Cuts at: 1645 3682 1645
Size: 2037 2093
BlpI GC'TnA_GC
Cuts at: 1242 1242
Size: 4130
Bme1580I G_kGCm'C
Cuts at: 172 1755 1848 2904 172
Size: 1583 93 1056 1398
Fragments arranged by size:
1583 1398 1056 93
BmgBI CAC'GTC
Cuts at: 156 156
Size: 4130
BmrI ACTGGGnnnn_n'
Cuts at: 1342 1423 1758 3902 1342
Size: 81 335 2144 1570
Fragments arranged by size:
2144 1570 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
Bpu10I CC'TnA_GC
Cuts at: 515 515
Size: 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 2185 2677 2975 1306
Size: 879 492 298 2461
Fragments arranged by size:
2461 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3834 3834
Size: 4130
BsaAI yAC'GTr
Cuts at: 1483 1483
Size: 4130
BsaBI GATnn'nnATC
Cuts at: 6 1644 3681 6
Size: 1638 2037 455
Fragments arranged by size:
2037 1638 455
BsaHI Gr'CG_yC
Cuts at: 1561 1809 2511 4007 1561
Size: 248 702 1496 1684
Fragments arranged by size:
1684 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1022 1319 1840 2518 2792 2939 3350 1022
Size: 297 521 678 274 147 411 1802
Fragments arranged by size:
1802 678 521 411 297 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 559 589 559
Size: 30 4100
BseYI C'CCAG_C
Cuts at: 423 483 656 1555 2313 2890 3386 423
Size: 60 173 899 758 577 496 1167
Fragments arranged by size:
1167 899 758 577 496 173 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1070
Size: 4130
BsiEI CG_ry'CG
Cuts at: 104 1672 1718 2926 104
Size: 1568 46 1208 1308
Fragments arranged by size:
1568 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 172 1144 1922 2112 2904 172
Size: 972 778 190 792 1398
Fragments arranged by size:
1398 972 792 778 190
BsmI GAATG_Cn'
Cuts at: 279 775 279
Size: 496 3634
BsmBI CGTCTCn'nnnn_
Cuts at: 128 128
Size: 4130
Bsp1286I G_dGCh'C
Cuts at: 172 1144 1755 1848 1922 2112 2174 2904 172
Size: 972 611 93 74 190 62 730 1398
Fragments arranged by size:
1398 972 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1022 3350 1022
Size: 2328 1802
BspHI T'CATG_A
Cuts at: 549 3306 549
Size: 2757 1373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1180 1394 1696 2077 1180
Size: 214 302 381 3233
Fragments arranged by size:
3233 381 302 214
BsrBI CCG'CTC
Cuts at: 50 346 2422 2476 3960 4125 50
Size: 296 2076 54 1484 165 55
Fragments arranged by size:
2076 1484 296 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2042 2042
Size: 4130
BsrFI r'CCGG_y
Cuts at: 2128 2309 2518 2128
Size: 181 209 3740
Fragments arranged by size:
3740 209 181
BssSI C'ACGA_G
Cuts at: 2401 2759 2401
Size: 358 3772
BstAPI GCAn_nnn'nTGC
Cuts at: 263 1507 263
Size: 1244 2886
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstYI r'GATC_y
Cuts at: 1 601 1155 1622 1645 1980 2226 3238 3324
Size: 600 554 467 23 335 246 1012 86
Cuts at: 3324 3336 3682 1
Size: 12 346 449
Fragments arranged by size:
1012 600 554 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2080 2261 2424 2080
Size: 181 163 3786
Fragments arranged by size:
3786 181 163
BtsI GCAGTG_nn'
Cuts at: 384 389 837 1058 1091 1373 384
Size: 5 448 221 33 282 3141
Fragments arranged by size:
3141 448 282 221 33 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 378 413 378
Size: 35 4095
DraI TTT'AAA
Cuts at: 698 1129 698
Size: 431 3699
DrdI GACnn_nn'nnGTC
Cuts at: 1836 2694 1836
Size: 858 3272
EaeI y'GGCC_r
Cuts at: 1083 1715 1889 2280 2307 3652 1083
Size: 632 174 391 27 1345 1561
Fragments arranged by size:
1561 1345 632 391 174 27
EagI C'GGCC_G
Cuts at: 1715 1715
Size: 4130
EarI CTCTTCn'nnn_
Cuts at: 662 2153 2363 662
Size: 1491 210 2429
Fragments arranged by size:
2429 1491 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2338 2648 2794 3940 2338
Size: 310 146 1146 2528
Fragments arranged by size:
2528 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1142 1142
Size: 4130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1955 2387 3134 3402 1955
Size: 432 747 268 2683
Fragments arranged by size:
2683 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1269 1269
Size: 4130
EcoRI G'AATT_C
Cuts at: 520 1107 1134 520
Size: 587 27 3516
Fragments arranged by size:
3516 587 27
EcoRV GAT'ATC
Cuts at: 147 147
Size: 4130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 4130
FspI TGC'GCA
Cuts at: 1911 1911
Size: 4130
HaeII r_GCGC'y
Cuts at: 1564 1812 2834 3813 1564
Size: 248 1022 979 1881
Fragments arranged by size:
1881 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 427 459 586 618 1972 2002 2004 2034 427
Size: 32 127 32 1354 30 2 30 2523
Fragments arranged by size:
2523 1354 127 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1169 1169
Size: 4130
KasI G'GCGC_C
Cuts at: 1560 1808 1560
Size: 248 3882
KpnI G_GTAC'C
Cuts at: 1150 1150
Size: 4130
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2801 2985 2801
Size: 184 3946
MscI TGG'CCA
Cuts at: 1085 1891 1085
Size: 806 3324
MslI CAynn'nnrTG
Cuts at: 1067 2119 2246 1067
Size: 1052 127 2951
Fragments arranged by size:
2951 1052 127
MspA1I CmG'CkG
Cuts at: 309 660 1241 1555 1915 2928 3173 309
Size: 351 581 314 360 1013 245 1266
Fragments arranged by size:
1266 1013 581 360 351 314 245
NaeI GCC'GGC
Cuts at: 2311 2311
Size: 4130
NarI GG'CG_CC
Cuts at: 1561 1809 1561
Size: 248 3882
NdeI CA'TA_TG
Cuts at: 591 591
Size: 4130
NgoMIV G'CCGG_C
Cuts at: 2309 2309
Size: 4130
NspI r_CATG'y
Cuts at: 266 420 1183 1683 2590 266
Size: 154 763 500 907 1806
Fragments arranged by size:
1806 907 763 500 154
PciI A'CATG_T
Cuts at: 416 2586 416
Size: 2170 1960
PfoI T'CCnGG_A
Cuts at: 577 577
Size: 4130
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 4098
PstI C_TGCA'G
Cuts at: 135 298 622 1177 1862 135
Size: 163 324 555 685 2403
Fragments arranged by size:
2403 685 555 324 163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 660 1555 1915 660
Size: 895 360 2875
Fragments arranged by size:
2875 895 360
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 1144 1144
Size: 4130
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 662 2153 2363 662
Size: 1491 210 2429
Fragments arranged by size:
2429 1491 210
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SfcI C'TryA_G
Cuts at: 33 131 294 618 1173 1858 2851 3042 33
Size: 98 163 324 555 685 993 191 1121
Fragments arranged by size:
1121 993 685 555 324 191 163 98
SfoI GGC'GCC
Cuts at: 1562 1810 1562
Size: 248 3882
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SmaI CCC'GGG
Cuts at: 1152 1152
Size: 4130
SmlI C'TyrA_G
Cuts at: 1285 2200 2692 2954 1285
Size: 915 492 262 2461
Fragments arranged by size:
2461 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 1183 1683 1183
Size: 500 3630
SspI AAT'ATT
Cuts at: 455 455
Size: 4130
StyI C'CwwG_G
Cuts at: 1264 1618 1264
Size: 354 3776
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 670 1132 1713 1846 2305 2514 670
Size: 462 581 133 459 209 2286
Fragments arranged by size:
2286 581 462 459 209 133
TatI w'GTAC_w
Cuts at: 3645 3645
Size: 4130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4098
Tth111I GACn'n_nGTC
Cuts at: 439 1927 439
Size: 1488 2642
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 455 1625 455
Size: 1170 2960
XmaI C'CCGG_G
Cuts at: 1150 1150
Size: 4130
XmnI GAAnn'nnTTC
Cuts at: 320 320
Size: 4130
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BpmI
BseRI BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI