Restriction map for
pF1KB7360
(Circular) (Six-base) MAPSORT of: pF1KB7360.seq Check: 678 from: 1 to: 4034
pF1KB7360 4034 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1072 1072
Size: 4034
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 3874 3874
Size: 4034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 876 1859 2291 3038 3306 876
Size: 983 432 747 268 1604
Fragments arranged by size:
1604 983 747 432 268
AflIII A'CryG_T
Cuts at: 1386 2490 3467 1386
Size: 1104 977 1953
Fragments arranged by size:
1953 1104 977
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AleI CACnn'nnGTG
Cuts at: 685 732 685
Size: 47 3987
AlwNI CAG_nnn'CTG
Cuts at: 183 265 395 1339 2906 183
Size: 82 130 944 1567 1311
Fragments arranged by size:
1567 1311 944 130 82
ApaLI G'TGCA_C
Cuts at: 2804 2804
Size: 4034
ApoI r'AATT_y
Cuts at: 139 231 1038 3387 3643 139
Size: 92 807 2349 256 530
Fragments arranged by size:
2349 807 530 256 92
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 1054 1054
Size: 4034
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 1050 1464 1712 1747 1050
Size: 414 248 35 3337
Fragments arranged by size:
3337 414 248 35
BanII G_rGCy'C
Cuts at: 272 1048 2078 272
Size: 776 1030 2228
Fragments arranged by size:
2228 1030 776
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BbsI GAAGACnn'nnnn_
Cuts at: 699 782 699
Size: 83 3951
BciVI GTATCCnnnnn_n'
Cuts at: 1932 2693 4034 1932
Size: 761 1341 1932
Fragments arranged by size:
1932 1341 761
BclI T'GATC_A
Cuts at: 1554 1554
Size: 4034
BfrBI ATG'CAT
Cuts at: 319 319
Size: 4034
BglII A'GATC_T
Cuts at: 1549 3586 1549
Size: 2037 1997
BlpI GC'TnA_GC
Cuts at: 1146 1146
Size: 4034
Bme1580I G_kGCm'C
Cuts at: 1659 1752 2808 1659
Size: 93 1056 2885
Fragments arranged by size:
2885 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 156 1246 1327 1662 3806 156
Size: 1090 81 335 2144 384
Fragments arranged by size:
2144 1090 384 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 1210 2089 2581 2879 152
Size: 1058 879 492 298 1307
Fragments arranged by size:
1307 1058 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3738 3738
Size: 4034
BsaAI yAC'GTr
Cuts at: 824 1387 824
Size: 563 3471
BsaBI GATnn'nnATC
Cuts at: 6 1548 3585 6
Size: 1542 2037 455
Fragments arranged by size:
2037 1542 455
BsaHI Gr'CG_yC
Cuts at: 1465 1713 2415 3911 1465
Size: 248 702 1496 1588
Fragments arranged by size:
1588 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1223 1744 2422 2696 2843 3254 1223
Size: 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 446 446
Size: 4034
BseYI C'CCAG_C
Cuts at: 903 1459 2217 2794 3290 903
Size: 556 758 577 496 1647
Fragments arranged by size:
1647 758 577 556 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 657 481
Size: 176 3858
BsiEI CG_ry'CG
Cuts at: 104 1576 1622 2830 104
Size: 1472 46 1208 1308
Fragments arranged by size:
1472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1048 1826 2016 2808 1048
Size: 778 190 792 2274
Fragments arranged by size:
2274 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 272 1048 1659 1752 1826 2016 2078 2808 272
Size: 776 611 93 74 190 62 730 1498
Fragments arranged by size:
1498 776 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 512 3210 512
Size: 2698 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 535 1084 1298 1600 1981 535
Size: 549 214 302 381 2588
Fragments arranged by size:
2588 549 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2326 2380 3864 4029 50
Size: 2276 54 1484 165 55
Fragments arranged by size:
2276 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 542 1946 542
Size: 1404 2630
BsrFI r'CCGG_y
Cuts at: 488 2032 2213 2422 488
Size: 1544 181 209 2100
Fragments arranged by size:
2100 1544 209 181
BssSI C'ACGA_G
Cuts at: 2305 2663 2305
Size: 358 3676
BstAPI GCAn_nnn'nTGC
Cuts at: 1411 1411
Size: 4034
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstYI r'GATC_y
Cuts at: 1 1059 1526 1549 1884 2130 3142 3228 3240
Size: 1058 467 23 335 246 1012 86 12
Cuts at: 3240 3586 1
Size: 346 449
Fragments arranged by size:
1058 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4034
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 462 1984 2165 2328 462
Size: 1522 181 163 2168
Fragments arranged by size:
2168 1522 181 163
BtsI GCAGTG_nn'
Cuts at: 1277 1277
Size: 4034
DraI TTT'AAA
Cuts at: 1033 1033
Size: 4034
DraIII CAC_nnn'GTG
Cuts at: 936 936
Size: 4034
DrdI GACnn_nn'nnGTC
Cuts at: 1740 2598 1740
Size: 858 3176
EaeI y'GGCC_r
Cuts at: 1619 1793 2184 2211 3556 1619
Size: 174 391 27 1345 2097
Fragments arranged by size:
2097 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1619 1619
Size: 4034
EarI CTCTTCn'nnn_
Cuts at: 931 2057 2267 931
Size: 1126 210 2698
Fragments arranged by size:
2698 1126 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2242 2552 2698 3844 2242
Size: 310 146 1146 2432
Fragments arranged by size:
2432 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1046 1046
Size: 4034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 876 1859 2291 3038 3306 876
Size: 983 432 747 268 1604
Fragments arranged by size:
1604 983 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1173 1173
Size: 4034
EcoRI G'AATT_C
Cuts at: 139 1038 139
Size: 899 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 4034
FspI TGC'GCA
Cuts at: 1815 1815
Size: 4034
HaeII r_GCGC'y
Cuts at: 1468 1716 2738 3717 1468
Size: 248 1022 979 1785
Fragments arranged by size:
1785 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 150 160 182 349 381 383 415 531
Size: 22 10 22 167 32 2 32 116
Cuts at: 531 563 854 886 1876 1906 1908 1938 128
Size: 32 291 32 990 30 2 30 2224
Fragments arranged by size:
2224 990 291 167 116 32 32 32
32 30 30 22 22 10 2 2
HincII GTy'rAC
Cuts at: 1073 1073
Size: 4034
HindIII A'AGCT_T
Cuts at: 992 992
Size: 4034
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2705 2889 2705
Size: 184 3850
MscI TGG'CCA
Cuts at: 1795 1795
Size: 4034
MslI CAynn'nnrTG
Cuts at: 685 732 2023 2150 685
Size: 47 1291 127 2569
Fragments arranged by size:
2569 1291 127 47
MspA1I CmG'CkG
Cuts at: 627 907 1145 1459 1819 2832 3077 627
Size: 280 238 314 360 1013 245 1584
Fragments arranged by size:
1584 1013 360 314 280 245 238
NaeI GCC'GGC
Cuts at: 490 2215 490
Size: 1725 2309
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NcoI C'CATG_G
Cuts at: 107 107
Size: 4034
NgoMIV G'CCGG_C
Cuts at: 488 2213 488
Size: 1725 2309
NsiI A_TGCA'T
Cuts at: 321 321
Size: 4034
NspI r_CATG'y
Cuts at: 1087 1587 2494 1087
Size: 500 907 2627
Fragments arranged by size:
2627 907 500
PasI CC'CwG_GG
Cuts at: 265 265
Size: 4034
PciI A'CATG_T
Cuts at: 2490 2490
Size: 4034
PfoI T'CCnGG_A
Cuts at: 311 311
Size: 4034
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PmlI CAC'GTG
Cuts at: 824 824
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PstI C_TGCA'G
Cuts at: 1081 1766 1081
Size: 685 3349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 627 907 1459 1819 627
Size: 280 552 360 2842
Fragments arranged by size:
2842 552 360 280
RsrII CG'GwC_CG
Cuts at: 2229 2229
Size: 4034
SacI G_AGCT'C
Cuts at: 1048 1048
Size: 4034
SalI G'TCGA_C
Cuts at: 1071 1071
Size: 4034
SapI GCTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 3551 3551
Size: 4034
SfcI C'TryA_G
Cuts at: 33 387 1077 1762 2755 2946 33
Size: 354 690 685 993 191 1121
Fragments arranged by size:
1121 993 690 685 354 191
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SmaI CCC'GGG
Cuts at: 1056 1056
Size: 4034
SmlI C'TyrA_G
Cuts at: 167 1189 2104 2596 2858 167
Size: 1022 915 492 262 1343
Fragments arranged by size:
1343 1022 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
SspI AAT'ATT
Cuts at: 206 206
Size: 4034
StyI C'CwwG_G
Cuts at: 107 150 426 1168 1522 107
Size: 43 276 742 354 2619
Fragments arranged by size:
2619 742 354 276 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 847 1617 1750 2209 2418 847
Size: 770 133 459 209 2463
Fragments arranged by size:
2463 770 459 209 133
TatI w'GTAC_w
Cuts at: 3549 3549
Size: 4034
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4002
Tth111I GACn'n_nGTC
Cuts at: 162 1831 162
Size: 1669 2365
XbaI T'CTAG_A
Cuts at: 1065 1065
Size: 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 582 1529 582
Size: 947 3087
XmaI C'CCGG_G
Cuts at: 1054 1054
Size: 4034
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PfoI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PflMI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI