Restriction map for
pF1KB7373
(Circular) (Six-base) MAPSORT of: pF1KB7373.seq Check: 3614 from: 1 to: 3857
pF1KB7373 3857 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 895 895
Size: 3857
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 2114 2861 3129 1682
Size: 432 747 268 2410
Fragments arranged by size:
2410 747 432 268
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 670 1162 2729 670
Size: 492 1567 1798
Fragments arranged by size:
1798 1567 492
ApaLI G'TGCA_C
Cuts at: 2627 2627
Size: 3857
ApoI r'AATT_y
Cuts at: 725 861 3210 3466 725
Size: 136 2349 256 1116
Fragments arranged by size:
2349 1116 256 136
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 877 877
Size: 3857
BamHI G'GATC_C
Cuts at: 882 882
Size: 3857
BanI G'GyrC_C
Cuts at: 873 1287 1535 1570 873
Size: 414 248 35 3160
Fragments arranged by size:
3160 414 248 35
BanII G_rGCy'C
Cuts at: 533 871 1901 533
Size: 338 1030 2489
Fragments arranged by size:
2489 1030 338
BbeI G_GCGC'C
Cuts at: 1291 1539 1291
Size: 248 3609
BbsI GAAGACnn'nnnn_
Cuts at: 192 192
Size: 3857
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 1377 1377
Size: 3857
BglII A'GATC_T
Cuts at: 1372 3409 1372
Size: 2037 1820
BlpI GC'TnA_GC
Cuts at: 969 969
Size: 3857
Bme1580I G_kGCm'C
Cuts at: 1482 1575 2631 1482
Size: 93 1056 2708
Fragments arranged by size:
2708 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 155 1069 1150 1485 3629 155
Size: 914 81 335 2144 383
Fragments arranged by size:
2144 914 383 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 3857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 165
Size: 3857
Bpu10I CC'TnA_GC
Cuts at: 527 527
Size: 3857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 1033 1912 2404 2702 255
Size: 778 879 492 298 1410
Fragments arranged by size:
1410 879 778 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3561 3561
Size: 3857
BsaAI yAC'GTr
Cuts at: 1210 1210
Size: 3857
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 1288 1536 2238 3734 1288
Size: 248 702 1496 1411
Fragments arranged by size:
1496 1411 702 248
BsaWI w'CCGG_w
Cuts at: 1046 1567 2245 2519 2666 3077 1046
Size: 521 678 274 147 411 1826
Fragments arranged by size:
1826 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 627 627
Size: 3857
BseYI C'CCAG_C
Cuts at: 807 1282 2040 2617 3113 807
Size: 475 758 577 496 1551
Fragments arranged by size:
1551 758 577 496 475
BsiEI CG_ry'CG
Cuts at: 104 1399 1445 2653 104
Size: 1295 46 1208 1308
Fragments arranged by size:
1308 1295 1208 46
BsiHKAI G_wGCw'C
Cuts at: 871 1649 1839 2631 871
Size: 778 190 792 2097
Fragments arranged by size:
2097 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 533 871 1482 1575 1649 1839 1901 2631 533
Size: 338 611 93 74 190 62 730 1759
Fragments arranged by size:
1759 730 611 338 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3077 3077
Size: 3857
BspHI T'CATG_A
Cuts at: 3033 3033
Size: 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 907 1121 1423 1804 907
Size: 214 302 381 2960
Fragments arranged by size:
2960 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1769 1769
Size: 3857
BsrFI r'CCGG_y
Cuts at: 1855 2036 2245 1855
Size: 181 209 3467
Fragments arranged by size:
3467 209 181
BssSI C'ACGA_G
Cuts at: 2128 2486 2128
Size: 358 3499
BstAPI GCAn_nnn'nTGC
Cuts at: 1234 1234
Size: 3857
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstYI r'GATC_y
Cuts at: 1 882 1349 1372 1707 1953 2965 3051 3063
Size: 881 467 23 335 246 1012 86 12
Cuts at: 3063 3409 1
Size: 346 449
Fragments arranged by size:
1012 881 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1807 1988 2151 1807
Size: 181 163 3513
Fragments arranged by size:
3513 181 163
BtsI GCAGTG_nn'
Cuts at: 584 1100 584
Size: 516 3341
DraI TTT'AAA
Cuts at: 816 856 816
Size: 40 3817
DraIII CAC_nnn'GTG
Cuts at: 163 163
Size: 3857
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 201 1442 1616 2007 2034 3379 201
Size: 1241 174 391 27 1345 679
Fragments arranged by size:
1345 1241 679 391 174 27
EagI C'GGCC_G
Cuts at: 1442 1442
Size: 3857
EarI CTCTTCn'nnn_
Cuts at: 299 660 1880 2090 299
Size: 361 1220 210 2066
Fragments arranged by size:
2066 1220 361 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2065 2375 2521 3667 2065
Size: 310 146 1146 2255
Fragments arranged by size:
2255 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 1682 2114 2861 3129 165
Size: 1517 432 747 268 893
Fragments arranged by size:
1517 893 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 451 996 451
Size: 545 3312
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 1291 1539 2561 3540 1291
Size: 248 1022 979 1608
Fragments arranged by size:
1608 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1699 1729 1731 1761 1699
Size: 30 2 30 3795
Fragments arranged by size:
3795 30 30 2
HincII GTy'rAC
Cuts at: 896 896
Size: 3857
KasI G'GCGC_C
Cuts at: 1287 1535 1287
Size: 248 3609
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 809 2528 2712 809
Size: 1719 184 1954
Fragments arranged by size:
1954 1719 184
MscI TGG'CCA
Cuts at: 203 1618 203
Size: 1415 2442
MslI CAynn'nnrTG
Cuts at: 1846 1973 1846
Size: 127 3730
MspA1I CmG'CkG
Cuts at: 207 968 1282 1642 2655 2900 207
Size: 761 314 360 1013 245 1164
Fragments arranged by size:
1164 1013 761 360 314 245
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 3857
NarI GG'CG_CC
Cuts at: 1288 1536 1288
Size: 248 3609
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 3857
NspI r_CATG'y
Cuts at: 910 1410 2317 910
Size: 500 907 2450
Fragments arranged by size:
2450 907 500
PasI CC'CwG_GG
Cuts at: 307 307
Size: 3857
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PpuMI rG'GwC_Cy
Cuts at: 451 451
Size: 3857
PstI C_TGCA'G
Cuts at: 904 1589 904
Size: 685 3172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 207 1282 1642 207
Size: 1075 360 2422
Fragments arranged by size:
2422 1075 360
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 1880 2090 1880
Size: 210 3647
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SfcI C'TryA_G
Cuts at: 33 225 900 1585 2578 2769 33
Size: 192 675 685 993 191 1121
Fragments arranged by size:
1121 993 685 675 192 191
SfoI GGC'GCC
Cuts at: 1289 1537 1289
Size: 248 3609
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SmaI CCC'GGG
Cuts at: 879 879
Size: 3857
SmlI C'TyrA_G
Cuts at: 270 1012 1927 2419 2681 270
Size: 742 915 492 262 1446
Fragments arranged by size:
1446 915 742 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 910 1410 910
Size: 500 3357
StyI C'CwwG_G
Cuts at: 991 1345 991
Size: 354 3503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 174 1440 1573 2032 2241 174
Size: 1266 133 459 209 1790
Fragments arranged by size:
1790 1266 459 209 133
TatI w'GTAC_w
Cuts at: 3372 3372
Size: 3857
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 558 590 3374 3406 558
Size: 32 2784 32 1009
Fragments arranged by size:
2784 1009 32 32
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1352 1352
Size: 3857
XmaI C'CCGG_G
Cuts at: 877 877
Size: 3857
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI