Restriction map for
pF1KB7374
(Circular) (Six-base) MAPSORT of: pF1KB7374.seq Check: 6210 from: 1 to: 3791
pF1KB7374 3791 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 730 3631 730
Size: 2901 890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 311 357 488 1616 2048 2795 3063 188
Size: 123 46 131 1128 432 747 268 916
Fragments arranged by size:
1128 916 747 432 268 131 123 46
AflII C'TTAA_G
Cuts at: 610 610
Size: 3791
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AlwNI CAG_nnn'CTG
Cuts at: 139 1096 2663 139
Size: 957 1567 1267
Fragments arranged by size:
1567 1267 957
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 183 285 592 795 3144 3400 183
Size: 102 307 203 2349 256 574
Fragments arranged by size:
2349 574 307 256 203 102
AseI AT'TA_AT
Cuts at: 20 505 20
Size: 485 3306
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 811 811
Size: 3791
AvrII C'CTAG_G
Cuts at: 118 118
Size: 3791
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 807 1221 1469 1504 807
Size: 414 248 35 3094
Fragments arranged by size:
3094 414 248 35
BanII G_rGCy'C
Cuts at: 805 1835 805
Size: 1030 2761
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BbsI GAAGACnn'nnnn_
Cuts at: 104 182 104
Size: 78 3713
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 903 903
Size: 3791
Bme1580I G_kGCm'C
Cuts at: 1416 1509 2565 1416
Size: 93 1056 2642
Fragments arranged by size:
2642 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1003 1084 1419 3563 1003
Size: 81 335 2144 1231
Fragments arranged by size:
2144 1231 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 651 2158 651
Size: 1507 2284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 967 1846 2338 2636 577
Size: 390 879 492 298 1732
Fragments arranged by size:
1732 879 492 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 418
Size: 3791
BseYI C'CCAG_C
Cuts at: 346 1216 1974 2551 3047 346
Size: 870 758 577 496 1090
Fragments arranged by size:
1090 870 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 805 1583 1773 2565 805
Size: 778 190 792 2031
Fragments arranged by size:
2031 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 805 1416 1509 1583 1773 1835 2565 805
Size: 611 93 74 190 62 730 2031
Fragments arranged by size:
2031 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 519 2967 519
Size: 2448 1343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1055 1357 1738 841
Size: 214 302 381 2894
Fragments arranged by size:
2894 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 1703
Size: 3791
BsrFI r'CCGG_y
Cuts at: 1789 1970 2179 1789
Size: 181 209 3401
Fragments arranged by size:
3401 209 181
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstYI r'GATC_y
Cuts at: 1 816 1283 1306 1641 1887 2899 2985 2997
Size: 815 467 23 335 246 1012 86 12
Cuts at: 2997 3343 1
Size: 346 449
Fragments arranged by size:
1012 815 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 345 399 1034 345
Size: 54 635 3102
Fragments arranged by size:
3102 635 54
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 499 1376 1550 1941 1968 3313 499
Size: 877 174 391 27 1345 977
Fragments arranged by size:
1345 977 877 391 174 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 284 314 372 1814 2024 284
Size: 30 58 1442 210 2051
Fragments arranged by size:
2051 1442 210 58 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 311 357 488 1616 2048 2795 3063 188
Size: 123 46 131 1128 432 747 268 916
Fragments arranged by size:
1128 916 747 432 268 131 123 46
EcoO109I rG'GnC_Cy
Cuts at: 367 930 367
Size: 563 3228
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
EcoRV GAT'ATC
Cuts at: 582 582
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1810 1810
Size: 3791
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 1225 1473 2495 3474 1225
Size: 248 1022 979 1542
Fragments arranged by size:
1542 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1633 1663 1665 1695 1633
Size: 30 2 30 3729
Fragments arranged by size:
3729 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2462 2646 2462
Size: 184 3607
MscI TGG'CCA
Cuts at: 1552 1552
Size: 3791
MslI CAynn'nnrTG
Cuts at: 1780 1907 1780
Size: 127 3664
MspA1I CmG'CkG
Cuts at: 902 1216 1576 2589 2834 902
Size: 314 360 1013 245 1859
Fragments arranged by size:
1859 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1972 1972
Size: 3791
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NgoMIV G'CCGG_C
Cuts at: 1970 1970
Size: 3791
NspI r_CATG'y
Cuts at: 354 844 1344 2251 354
Size: 490 500 907 1894
Fragments arranged by size:
1894 907 500 490
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PflMI CCAn_nnn'nTGG
Cuts at: 353 353
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PstI C_TGCA'G
Cuts at: 414 838 1523 414
Size: 424 685 2682
Fragments arranged by size:
2682 685 424
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 1216 1576 1216
Size: 360 3431
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 314 1814 2024 314
Size: 1500 210 2081
Fragments arranged by size:
2081 1500 210
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 410 834 1519 2512 2703 33
Size: 377 424 685 993 191 1121
Fragments arranged by size:
1121 993 685 424 377 191
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 556 610 946 1861 2353 2615 556
Size: 54 336 915 492 262 1732
Fragments arranged by size:
1732 915 492 336 262 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
SspI AAT'ATT
Cuts at: 509 509
Size: 3791
StyI C'CwwG_G
Cuts at: 118 925 1279 118
Size: 807 354 2630
Fragments arranged by size:
2630 807 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 3306 3306
Size: 3791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1286 1286
Size: 3791
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI