Restriction map for
pF1KB7384
(Circular) (Six-base) MAPSORT of: pF1KB7384.seq Check: 3417 from: 1 to: 4505
pF1KB7384 4505 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1543 1543
Size: 4505
Acc65I G'GTAC_C
Cuts at: 754 1270 1521 754
Size: 516 251 3738
Fragments arranged by size:
3738 516 251
AclI AA'CG_TT
Cuts at: 4345 4345
Size: 4505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2762 3509 3777 2330
Size: 432 747 268 3058
Fragments arranged by size:
3058 747 432 268
AflIII A'CryG_T
Cuts at: 311 1857 2961 3938 311
Size: 1546 1104 977 878
Fragments arranged by size:
1546 1104 977 878
AgeI A'CCGG_T
Cuts at: 2893 2893
Size: 4505
AleI CACnn'nnGTG
Cuts at: 1343 1343
Size: 4505
AlwNI CAG_nnn'CTG
Cuts at: 1810 3377 1810
Size: 1567 2938
ApaLI G'TGCA_C
Cuts at: 3275 3275
Size: 4505
ApoI r'AATT_y
Cuts at: 575 1490 1509 3858 4114 575
Size: 915 19 2349 256 966
Fragments arranged by size:
2349 966 915 256 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
AvaI C'yCGr_G
Cuts at: 1525 1525
Size: 4505
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 738 746 771 779 738
Size: 8 25 8 4464
Fragments arranged by size:
4464 25 8 8
BamHI G'GATC_C
Cuts at: 1530 1530
Size: 4505
BanI G'GyrC_C
Cuts at: 234 754 1270 1521 1935 2183 2218 234
Size: 520 516 251 414 248 35 2521
Fragments arranged by size:
2521 520 516 414 251 248 35
BanII G_rGCy'C
Cuts at: 347 725 1519 2549 347
Size: 378 794 1030 2303
Fragments arranged by size:
2303 1030 794 378
BbeI G_GCGC'C
Cuts at: 1939 2187 1939
Size: 248 4257
BbsI GAAGACnn'nnnn_
Cuts at: 998 1120 998
Size: 122 4383
BciVI GTATCCnnnnn_n'
Cuts at: 2403 3164 4505 2403
Size: 761 1341 2403
Fragments arranged by size:
2403 1341 761
BclI T'GATC_A
Cuts at: 2025 2025
Size: 4505
BfrBI ATG'CAT
Cuts at: 690 690
Size: 4505
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4505
BglII A'GATC_T
Cuts at: 1427 2020 4057 1427
Size: 593 2037 1875
Fragments arranged by size:
2037 1875 593
BlpI GC'TnA_GC
Cuts at: 1617 1617
Size: 4505
Bme1580I G_kGCm'C
Cuts at: 239 2130 2223 3279 239
Size: 1891 93 1056 1465
Fragments arranged by size:
1891 1465 1056 93
BmgBI CAC'GTC
Cuts at: 252 252
Size: 4505
BmrI ACTGGGnnnn_n'
Cuts at: 1717 1798 2133 4277 1717
Size: 81 335 2144 1945
Fragments arranged by size:
2144 1945 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 418 420 438 2872 418
Size: 2 18 2434 2051
Fragments arranged by size:
2434 2051 18 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1304 1304
Size: 4505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 2560 3052 3350 1681
Size: 879 492 298 2836
Fragments arranged by size:
2836 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4209 4209
Size: 4505
BsaAI yAC'GTr
Cuts at: 314 1858 314
Size: 1544 2961
BsaBI GATnn'nnATC
Cuts at: 6 2019 4056 6
Size: 2013 2037 455
Fragments arranged by size:
2037 2013 455
BsaHI Gr'CG_yC
Cuts at: 1936 2184 2886 4382 1936
Size: 248 702 1496 2059
Fragments arranged by size:
2059 1496 702 248
BsaWI w'CCGG_w
Cuts at: 197 1694 2215 2893 3167 3314 3725 197
Size: 1497 521 678 274 147 411 977
Fragments arranged by size:
1497 977 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 792 1136 1275 792
Size: 344 139 4022
Fragments arranged by size:
4022 344 139
BseYI C'CCAG_C
Cuts at: 238 1930 2688 3265 3761 238
Size: 1692 758 577 496 982
Fragments arranged by size:
1692 982 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 167 270 2047 2093 3301 104
Size: 63 103 1777 46 1208 1308
Fragments arranged by size:
1777 1308 1208 103 63 46
BsiHKAI G_wGCw'C
Cuts at: 251 419 672 725 1519 2297 2487 3279 251
Size: 168 253 53 794 778 190 792 1477
Fragments arranged by size:
1477 794 792 778 253 190 168 53
Bsp1286I G_dGCh'C
Cuts at: 239 251 347 419 672 725 1519 2130 2223
Size: 12 96 72 253 53 794 611 93
Cuts at: 2223 2297 2487 2549 3279 239
Size: 74 190 62 730 1465
Fragments arranged by size:
1465 794 730 611 253 190 96 93
74 72 62 53 12
BspEI T'CCGG_A
Cuts at: 197 3725 197
Size: 3528 977
BspHI T'CATG_A
Cuts at: 3681 3681
Size: 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1555 1769 2071 2452 1555
Size: 214 302 381 3608
Fragments arranged by size:
3608 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2797 2851 4335 4500 50
Size: 2747 54 1484 165 55
Fragments arranged by size:
2747 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2417 2417
Size: 4505
BsrFI r'CCGG_y
Cuts at: 2503 2684 2893 2503
Size: 181 209 4115
Fragments arranged by size:
4115 209 181
BssSI C'ACGA_G
Cuts at: 2776 3134 2776
Size: 358 4147
BstAPI GCAn_nnn'nTGC
Cuts at: 1882 1882
Size: 4505
BstBI TT'CG_AA
Cuts at: 2866 2866
Size: 4505
BstXI CCAn_nnnn'nTGG
Cuts at: 751 751
Size: 4505
BstYI r'GATC_y
Cuts at: 1 398 1427 1530 1997 2020 2355 2601 3613
Size: 397 1029 103 467 23 335 246 1012
Cuts at: 3613 3699 3711 4057 1
Size: 86 12 346 449
Fragments arranged by size:
1029 1012 467 449 397 346 335 246
103 86 23 12
BtgI C'CryG_G
Cuts at: 383 383
Size: 4505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 133 2455 2636 2799 133
Size: 2322 181 163 1839
Fragments arranged by size:
2322 1839 181 163
BtsI GCAGTG_nn'
Cuts at: 1748 1748
Size: 4505
DraI TTT'AAA
Cuts at: 1504 1504
Size: 4505
DraIII CAC_nnn'GTG
Cuts at: 224 224
Size: 4505
DrdI GACnn_nn'nnGTC
Cuts at: 2211 3069 2211
Size: 858 3647
EaeI y'GGCC_r
Cuts at: 164 267 386 1392 2090 2264 2655 2682 4027
Size: 103 119 1006 698 174 391 27 1345
Cuts at: 4027 164
Size: 642
Fragments arranged by size:
1345 1006 698 642 391 174 119 103
27
EagI C'GGCC_G
Cuts at: 164 267 2090 164
Size: 103 1823 2579
Fragments arranged by size:
2579 1823 103
EarI CTCTTCn'nnn_
Cuts at: 335 725 1293 1408 2528 2738 335
Size: 390 568 115 1120 210 2102
Fragments arranged by size:
2102 1120 568 390 210 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2713 3023 3169 4315 2713
Size: 310 146 1146 2903
Fragments arranged by size:
2903 1146 310 146
EcoICRI GAG'CTC
Cuts at: 723 1517 723
Size: 794 3711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1304 2330 2762 3509 3777 1304
Size: 1026 432 747 268 2032
Fragments arranged by size:
2032 1026 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1220 1220
Size: 4505
EcoO109I rG'GnC_Cy
Cuts at: 519 1644 519
Size: 1125 3380
EcoRI G'AATT_C
Cuts at: 575 1509 575
Size: 934 3571
EcoRV GAT'ATC
Cuts at: 839 839
Size: 4505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4505
FspI TGC'GCA
Cuts at: 2286 2286
Size: 4505
HaeII r_GCGC'y
Cuts at: 1939 2187 3209 4188 1939
Size: 248 1022 979 2256
Fragments arranged by size:
2256 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2347 2377 2379 2409 2347
Size: 30 2 30 4443
Fragments arranged by size:
4443 30 30 2
HincII GTy'rAC
Cuts at: 213 663 1005 1071 1544 213
Size: 450 342 66 473 3174
Fragments arranged by size:
3174 473 450 342 66
HindIII A'AGCT_T
Cuts at: 765 765
Size: 4505
HpaI GTT'AAC
Cuts at: 1071 1071
Size: 4505
KasI G'GCGC_C
Cuts at: 1935 2183 1935
Size: 248 4257
KpnI G_GTAC'C
Cuts at: 758 1274 1525 758
Size: 516 251 3738
Fragments arranged by size:
3738 516 251
MluI A'CGCG_T
Cuts at: 3938 3938
Size: 4505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 528 952 3176 3360 528
Size: 424 2224 184 1673
Fragments arranged by size:
2224 1673 424 184
MscI TGG'CCA
Cuts at: 388 1394 2266 388
Size: 1006 872 2627
Fragments arranged by size:
2627 1006 872
MslI CAynn'nnrTG
Cuts at: 749 1343 2494 2621 749
Size: 594 1151 127 2633
Fragments arranged by size:
2633 1151 594 127
MspA1I CmG'CkG
Cuts at: 409 1471 1616 1930 2290 3303 3548 409
Size: 1062 145 314 360 1013 245 1366
Fragments arranged by size:
1366 1062 1013 360 314 245 145
NaeI GCC'GGC
Cuts at: 2686 2686
Size: 4505
NarI GG'CG_CC
Cuts at: 1936 2184 1936
Size: 248 4257
NcoI C'CATG_G
Cuts at: 383 383
Size: 4505
NgoMIV G'CCGG_C
Cuts at: 2684 2684
Size: 4505
NotI GC'GGCC_GC
Cuts at: 164 164
Size: 4505
NsiI A_TGCA'T
Cuts at: 692 692
Size: 4505
NspI r_CATG'y
Cuts at: 1558 2058 2965 1558
Size: 500 907 3098
Fragments arranged by size:
3098 907 500
PasI CC'CwG_GG
Cuts at: 462 462
Size: 4505
PciI A'CATG_T
Cuts at: 2961 2961
Size: 4505
PflMI CCAn_nnn'nTGG
Cuts at: 1081 1081
Size: 4505
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 4473
PpuMI rG'GwC_Cy
Cuts at: 519 519
Size: 4505
PshAI GACnn'nnGTC
Cuts at: 923 923
Size: 4505
PstI C_TGCA'G
Cuts at: 414 1552 2237 414
Size: 1138 685 2682
Fragments arranged by size:
2682 1138 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4505
PvuII CAG'CTG
Cuts at: 409 1471 1930 2290 409
Size: 1062 459 360 2624
Fragments arranged by size:
2624 1062 459 360
RsrII CG'GwC_CG
Cuts at: 2700 2700
Size: 4505
SacI G_AGCT'C
Cuts at: 725 1519 725
Size: 794 3711
SalI G'TCGA_C
Cuts at: 1542 1542
Size: 4505
SanDI GG'GwC_CC
Cuts at: 519 519
Size: 4505
SapI GCTCTTCn'nnn_
Cuts at: 335 2528 2738 335
Size: 2193 210 2102
Fragments arranged by size:
2193 2102 210
SbfI CC_TGCA'GG
Cuts at: 1552 1552
Size: 4505
ScaI AGT'ACT
Cuts at: 4022 4022
Size: 4505
SfcI C'TryA_G
Cuts at: 33 410 884 980 1548 2233 3226 3417 33
Size: 377 474 96 568 685 993 191 1121
Fragments arranged by size:
1121 993 685 568 474 377 191 96
SfoI GGC'GCC
Cuts at: 1937 2185 1937
Size: 248 4257
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
SmaI CCC'GGG
Cuts at: 1527 1527
Size: 4505
SmlI C'TyrA_G
Cuts at: 1660 2575 3067 3329 1660
Size: 915 492 262 2836
Fragments arranged by size:
2836 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4505
SphI G_CATG'C
Cuts at: 1558 2058 1558
Size: 500 4005
StyI C'CwwG_G
Cuts at: 383 1639 1993 383
Size: 1256 354 2895
Fragments arranged by size:
2895 1256 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 680 2088 2221 2680 2889 680
Size: 1408 133 459 209 2296
Fragments arranged by size:
2296 1408 459 209 133
TatI w'GTAC_w
Cuts at: 1219 4020 1219
Size: 2801 1704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4022 4054 4022
Size: 32 4473
Tth111I GACn'n_nGTC
Cuts at: 2302 2302
Size: 4505
XbaI T'CTAG_A
Cuts at: 1536 1536
Size: 4505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2000 2000
Size: 4505
XmaI C'CCGG_G
Cuts at: 1525 1525
Size: 4505
XmnI GAAnn'nnTTC
Cuts at: 739 739
Size: 4505
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI FseI FspAI MfeI NdeI NheI NruI
PacI PfoI PmlI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI