Restriction map for
pF1KB7393
(Circular) (Six-base) MAPSORT of: pF1KB7393.seq Check: 3227 from: 1 to: 4355
pF1KB7393 4355 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1393 1393
Size: 4355
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4355
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 476 568 1304 2180 2612 3359 3627 283
Size: 193 92 736 876 432 747 268 1011
Fragments arranged by size:
1011 876 747 736 432 268 193 92
AflII C'TTAA_G
Cuts at: 859 859
Size: 4355
AflIII A'CryG_T
Cuts at: 1707 2811 3788 1707
Size: 1104 977 2274
Fragments arranged by size:
2274 1104 977
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AleI CACnn'nnGTG
Cuts at: 481 481
Size: 4355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1259 1259
Size: 4355
AlwNI CAG_nnn'CTG
Cuts at: 1660 3227 1660
Size: 1567 2788
ApaLI G'TGCA_C
Cuts at: 3125 3125
Size: 4355
ApoI r'AATT_y
Cuts at: 322 496 558 864 976 984 1172 1359 3708
Size: 174 62 306 112 8 188 187 2349
Cuts at: 3708 3964 322
Size: 256 713
Fragments arranged by size:
2349 713 306 256 188 187 174 112
62 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 4355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4355
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 449 482 449
Size: 33 4322
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4355
BanI G'GyrC_C
Cuts at: 1371 1785 2033 2068 1371
Size: 414 248 35 3658
Fragments arranged by size:
3658 414 248 35
BanII G_rGCy'C
Cuts at: 1369 2399 1369
Size: 1030 3325
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4107
BbsI GAAGACnn'nnnn_
Cuts at: 493 493
Size: 4355
BbvCI CC'TCA_GC
Cuts at: 805 805
Size: 4355
BciVI GTATCCnnnnn_n'
Cuts at: 1155 2253 3014 4355 1155
Size: 1098 761 1341 1155
Fragments arranged by size:
1341 1155 1098 761
BclI T'GATC_A
Cuts at: 134 1875 134
Size: 1741 2614
BglII A'GATC_T
Cuts at: 1870 3907 1870
Size: 2037 2318
BlpI GC'TnA_GC
Cuts at: 1467 1467
Size: 4355
Bme1580I G_kGCm'C
Cuts at: 1980 2073 3129 1980
Size: 93 1056 3206
Fragments arranged by size:
3206 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1567 1648 1983 4127 1567
Size: 81 335 2144 1795
Fragments arranged by size:
2144 1795 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 452 2722 452
Size: 2270 2085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 835 763
Size: 72 4283
Bpu10I CC'TnA_GC
Cuts at: 163 292 805 163
Size: 129 513 3713
Fragments arranged by size:
3713 513 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 678 1531 2410 2902 3200 678
Size: 853 879 492 298 1833
Fragments arranged by size:
1833 879 853 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 692 4059 692
Size: 3367 988
BsaAI yAC'GTr
Cuts at: 1708 1708
Size: 4355
BsaBI GATnn'nnATC
Cuts at: 6 1869 3906 6
Size: 1863 2037 455
Fragments arranged by size:
2037 1863 455
BsaHI Gr'CG_yC
Cuts at: 182 1786 2034 2736 4232 182
Size: 1604 248 702 1496 305
Fragments arranged by size:
1604 1496 702 305 248
BsaWI w'CCGG_w
Cuts at: 1544 2065 2743 3017 3164 3575 1544
Size: 521 678 274 147 411 2324
Fragments arranged by size:
2324 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1780 2538 3115 3611 1780
Size: 758 577 496 2524
Fragments arranged by size:
2524 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1073 856
Size: 217 4138
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3151 104
Size: 1793 46 1208 1308
Fragments arranged by size:
1793 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1369 2147 2337 3129 1369
Size: 778 190 792 2595
Fragments arranged by size:
2595 792 778 190
BsmI GAATG_Cn'
Cuts at: 1236 1236
Size: 4355
Bsp1286I G_dGCh'C
Cuts at: 1369 1980 2073 2147 2337 2399 3129 1369
Size: 611 93 74 190 62 730 2595
Fragments arranged by size:
2595 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3575 3575
Size: 4355
BspHI T'CATG_A
Cuts at: 3531 3531
Size: 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1405 1619 1921 2302 1405
Size: 214 302 381 3458
Fragments arranged by size:
3458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2647 2701 4185 4350 50
Size: 2597 54 1484 165 55
Fragments arranged by size:
2597 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2267 2267
Size: 4355
BsrFI r'CCGG_y
Cuts at: 140 231 2353 2534 2743 140
Size: 91 2122 181 209 1752
Fragments arranged by size:
2122 1752 209 181 91
BssSI C'ACGA_G
Cuts at: 2626 2984 2626
Size: 358 3997
BstAPI GCAn_nnn'nTGC
Cuts at: 1732 1732
Size: 4355
BstBI TT'CG_AA
Cuts at: 2716 2716
Size: 4355
BstYI r'GATC_y
Cuts at: 1 1005 1380 1847 1870 2205 2451 3463 3549
Size: 1004 375 467 23 335 246 1012 86
Cuts at: 3549 3561 3907 1
Size: 12 346 449
Fragments arranged by size:
1012 1004 467 449 375 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 643 643
Size: 4355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2305 2486 2649 2305
Size: 181 163 4011
Fragments arranged by size:
4011 181 163
BtsI GCAGTG_nn'
Cuts at: 1598 1598
Size: 4355
DraI TTT'AAA
Cuts at: 407 510 869 1354 407
Size: 103 359 485 3408
Fragments arranged by size:
3408 485 359 103
DraIII CAC_nnn'GTG
Cuts at: 1254 1254
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2919 2061
Size: 858 3497
EaeI y'GGCC_r
Cuts at: 1940 2114 2505 2532 3877 1940
Size: 174 391 27 1345 2418
Fragments arranged by size:
2418 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4355
EarI CTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 2873 3019 4165 2563
Size: 310 146 1146 2753
Fragments arranged by size:
2753 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1367 1367
Size: 4355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 476 568 763 835 1304 2180 2612 3359
Size: 193 92 195 72 469 876 432 747
Cuts at: 3359 3627 283
Size: 268 1011
Fragments arranged by size:
1011 876 747 469 432 268 195 193
92 72
EcoO109I rG'GnC_Cy
Cuts at: 278 1494 278
Size: 1216 3139
EcoRI G'AATT_C
Cuts at: 1359 1359
Size: 4355
EcoRV GAT'ATC
Cuts at: 375 375
Size: 4355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 433 2374 433
Size: 1941 2414
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4355
HaeII r_GCGC'y
Cuts at: 1789 2037 3059 4038 1789
Size: 248 1022 979 2106
Fragments arranged by size:
2106 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 2197 2227 2229 2259 189
Size: 32 1976 30 2 30 2285
Fragments arranged by size:
2285 1976 32 30 30 2
HincII GTy'rAC
Cuts at: 1394 1394
Size: 4355
HindIII A'AGCT_T
Cuts at: 679 955 679
Size: 276 4079
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4107
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4355
MfeI C'AATT_G
Cuts at: 692 692
Size: 4355
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3026 3210 3026
Size: 184 4171
MscI TGG'CCA
Cuts at: 2116 2116
Size: 4355
MslI CAynn'nnrTG
Cuts at: 481 2344 2471 481
Size: 1863 127 2365
Fragments arranged by size:
2365 1863 127
MspA1I CmG'CkG
Cuts at: 1466 1780 2140 3153 3398 1466
Size: 314 360 1013 245 2423
Fragments arranged by size:
2423 1013 360 314 245
NaeI GCC'GGC
Cuts at: 142 2536 142
Size: 2394 1961
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4107
NdeI CA'TA_TG
Cuts at: 1119 1119
Size: 4355
NgoMIV G'CCGG_C
Cuts at: 140 2534 140
Size: 2394 1961
NspI r_CATG'y
Cuts at: 1408 1908 2815 1408
Size: 500 907 2948
Fragments arranged by size:
2948 907 500
PciI A'CATG_T
Cuts at: 2811 2811
Size: 4355
PflMI CCAn_nnn'nTGG
Cuts at: 1158 1254 1158
Size: 96 4259
PfoI T'CCnGG_A
Cuts at: 217 851 217
Size: 634 3721
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 4323
PpuMI rG'GwC_Cy
Cuts at: 278 278
Size: 4355
PshAI GACnn'nnGTC
Cuts at: 659 659
Size: 4355
PstI C_TGCA'G
Cuts at: 1402 2087 1402
Size: 685 3670
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 1780 2140 1780
Size: 360 3995
RsrII CG'GwC_CG
Cuts at: 234 2550 234
Size: 2316 2039
SacI G_AGCT'C
Cuts at: 1369 1369
Size: 4355
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4355
SanDI GG'GwC_CC
Cuts at: 278 278
Size: 4355
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SfcI C'TryA_G
Cuts at: 33 1398 2083 3076 3267 33
Size: 1365 685 993 191 1121
Fragments arranged by size:
1365 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4355
SmlI C'TyrA_G
Cuts at: 657 859 1510 2425 2917 3179 657
Size: 202 651 915 492 262 1833
Fragments arranged by size:
1833 915 651 492 262 202
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 1408 1908 1408
Size: 500 3855
StyI C'CwwG_G
Cuts at: 155 572 1489 1843 155
Size: 417 917 354 2667
Fragments arranged by size:
2667 917 417 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1264 1938 2071 2530 2739 1264
Size: 674 133 459 209 2880
Fragments arranged by size:
2880 674 459 209 133
TatI w'GTAC_w
Cuts at: 3870 3870
Size: 4355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4323
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 1386 1386
Size: 4355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1850 1850
Size: 4355
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4355
XmnI GAAnn'nnTTC
Cuts at: 581 890 1204 581
Size: 309 314 3732
Fragments arranged by size:
3732 314 309
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BcgI BfrBI BglI BmgBI BmtI BsaXI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI EcoNI FseI FspAI HpaI NcoI NheI NotI
NruI NsiI PacI PasI PmlI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI